Restriction map for
pFN21AE0323
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# NO CUT ENZYMES ( 93 enzymes) #
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AatII Acc65I AclI AcsI AflII
AflIII AgeI Alw44I AocI ApaLI
ApoI AscI AseI AsnI Asp718I
AviII BbeI BbrPI BciVI BclI
BfrI BglI BsaAI BsaBI BsiEI
BsmBI Bsp1407I BspDI BspLU11I BsrBI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
FbaI FseI FspI HpaI KasI
KpnI MamI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1105 GTAGAC GT^[AC][GT]AC
AccIII 1796 TCCGGA T^CCGGA
AcyI 1599 GACGCC G[GA]^CG[CT]C
Aor51HI 695 AGCGCT AGC^GCT
ApaI 1307 GGGCCC GGGCC^C
Asp700I 303 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2250 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1982 GGATCC G^GATCC
BcgI 1611 GCAGACCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1432 AGATCT A^GATCT
BsaHI 1599 GACGCC G[GA]^CG[CT]C
BsaWI 1796 TCCGGA [AT]^CCGG[AT]
BseAI 1796 TCCGGA T^CCGGA
BsgI 2428 CTGCAC GTGCAG(16/14)
BsiWI 480 CGTACG C^GTACG
BspEI 1796 TCCGGA T^CCGGA
BspHI 704 TCATGA T^CATGA
BsrDI 494 GCAATG GCAATG(2/0)
BsrFI 1088 ACCGGC [GA]^CCGG[CT]
BssHII 2395 GCGCGC G^CGCGC
BstAPI 485 GCACTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 405 CCAGATCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1088 ACCGGC [GA]^CCGG[CT]
Eco47III 695 AGCGCT AGC^GCT
EcoNI 276 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1915 ATGCAT ATGCA^T
Hin1I 1599 GACGCC G[GA]^CG[CT]C
HincII 190 GTCAAC GT[CT]^[GA]AC
HindII 190 GTCAAC GT[CT]^[GA]AC
HindIII 304 AAGCTT A^AGCTT
KspI 342 CCGCGG CCGC^GG
MroI 1796 TCCGGA T^CCGGA
NcoI 1628 CCATGG C^CATGG
NsiI 1915 ATGCAT ATGCA^T
PflFI 2250 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1842 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 793 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 704 TCATGA T^CATGA
SacII 342 CCGCGG CCGC^GG
ScaI 152 AGTACT AGT^ACT
SmaI 1419 CCCGGG CCC^GGG
SphI 828 GCATGC GCATG^C
SspI 758 AATATT AAT^ATT
Tth111I 2250 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1842 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1419 CCCGGG C^CCGGG
XmaI 1419 CCCGGG C^CCGGG
XmnI 303 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AhdI 1108 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1894 GACCTGCAGTC
AlwNI 623 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2345 CAGAGTCTG
AspEI 1108 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1894 GACCTGCAGTC
AvrII 2245 CCTAGG C^CTAGG
2370 CCTAGG
BlnI 2245 CCTAGG C^CTAGG
2370 CCTAGG
BspMI 1895 ACCTGC ACCTGC(4/8)
2329 ACCTGC
Eam1105I 1108 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1894 GACCTGCAGTC
HaeII 695 AGCGCT [GA]GCGC^[CT]
1685 AGCGCC
HphI 3 GGTGA GGTGA(8/7)
368 TCACC
MaeII 1103 ACGT A^CGT
2408 ACGT
MseI 1778 TTAA T^TAA
2131 TTAA
PvuII 235 CAGCTG CAG^CTG
1069 CAGCTG
SacI 499 GAGCTC GAGCT^C
1353 GAGCTC
StuI 355 AGGCCT AGG^CCT
1659 AGGCCT
Tru9I 1778 TTAA T^TAA
2131 TTAA
Tsp509I 1017 AATT ^AATT
2174 AATT
XcmI 405 CCAGATCAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1628 CCATGGAATCCCTGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XhoII