(Circular) (Six-base) MAPSORT of: pFN21AE0323.seq Check: 8031 from: 1 to: 7237 pFN21AE0323 7237 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4343 4343 Size: 7237 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5047 278 Size: 53 83 186 936 3511 2468 Fragments arranged by size: 3511 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3111 4580 1937 Size: 1174 1469 4594 Fragments arranged by size: 4594 1469 1174 Acc65I G'GTAC_C Cuts at: 4558 4558 Size: 7237 AclI AA'CG_TT Cuts at: 1563 5364 5737 1563 Size: 3801 373 3063 Fragments arranged by size: 3801 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2156 2327 2597 3325 4332 5300 6684 6952 Size: 524 171 270 728 1007 968 1384 268 Cuts at: 6952 1632 Size: 1917 Fragments arranged by size: 1917 1384 1007 968 728 524 270 268 171 AfeI AGC'GCT Cuts at: 2702 2702 Size: 7237 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7040 AflIII A'CryG_T Cuts at: 1203 6136 7113 1203 Size: 4933 977 1327 Fragments arranged by size: 4933 1327 977 AgeI A'CCGG_T Cuts at: 6068 6068 Size: 7237 AhdI GACnn_n'nnGTC Cuts at: 3118 3904 5966 3118 Size: 786 2062 4389 Fragments arranged by size: 4389 2062 786 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7237 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2638 3980 1184 Size: 1454 1342 4441 Fragments arranged by size: 4441 1454 1342 AlwNI CAG_nnn'CTG Cuts at: 2633 4355 4980 6552 2633 Size: 1722 625 1572 3318 Fragments arranged by size: 3318 1722 1572 625 ApaI G_GGCC'C Cuts at: 3316 3316 Size: 7237 ApaLI G'TGCA_C Cuts at: 5294 6450 5294 Size: 1156 6081 ApoI r'AATT_y Cuts at: 1493 4546 4772 4924 7033 1493 Size: 3053 226 152 2109 1697 Fragments arranged by size: 3053 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5791 160 Size: 872 4759 1606 Fragments arranged by size: 4759 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7237 AvaI C'yCGr_G Cuts at: 1870 1943 3424 3486 3624 4562 1870 Size: 73 1481 62 138 938 4545 Fragments arranged by size: 4545 1481 938 138 73 62 AvrII C'CTAG_G Cuts at: 4250 4375 4250 Size: 125 7112 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2141 2174 2141 Size: 33 7204 BamHI G'GATC_C Cuts at: 3987 4567 4934 3987 Size: 580 367 6290 Fragments arranged by size: 6290 580 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2469 2559 3683 4558 6013 Size: 325 217 627 682 90 1124 875 1455 Cuts at: 6013 618 Size: 1842 Fragments arranged by size: 1842 1455 1124 875 682 627 325 217 90 BanII G_rGCy'C Cuts at: 1391 2508 3054 3204 3248 3316 3362 3632 4556 Size: 1117 546 150 44 68 46 270 924 Cuts at: 4556 1391 Size: 4072 Fragments arranged by size: 4072 1117 924 546 270 150 68 46 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2042 2728 2952 3420 928 Size: 971 143 686 224 468 4745 Fragments arranged by size: 4745 971 686 468 224 143 BbvCI CC'TCA_GC Cuts at: 2634 3260 3728 2634 Size: 626 468 6143 Fragments arranged by size: 6143 626 468 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3605 3639 5428 5462 1903 Size: 34 1668 34 1789 34 3678 Fragments arranged by size: 3678 1789 1668 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5129 6339 5129 Size: 1210 6027 BclI T'GATC_A Cuts at: 1548 1548 Size: 7237 BfrBI ATG'CAT Cuts at: 3922 3922 Size: 7237 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5848 136 Size: 107 122 71 631 605 4176 1525 Fragments arranged by size: 4176 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3437 7232 3437 Size: 3795 3442 BlpI GC'TnA_GC Cuts at: 3140 3764 3875 4654 3140 Size: 624 111 779 5723 Fragments arranged by size: 5723 779 624 111 Bme1580I G_kGCm'C Cuts at: 1790 2782 3316 3779 4250 5298 6454 1790 Size: 992 534 463 471 1048 1156 2573 Fragments arranged by size: 2573 1156 1048 992 534 471 463 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7237 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2363 4968 5926 Size: 416 53 83 399 24 946 2605 958 Cuts at: 5926 442 Size: 1753 Fragments arranged by size: 2605 1753 958 946 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7237 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4144 4144 Size: 7237 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 2226 3205 5897 1239 Size: 806 181 979 2692 2579 Fragments arranged by size: 2692 2579 979 806 181 Bpu10I CC'TnA_GC Cuts at: 2634 3260 3728 2634 Size: 626 468 6143 Fragments arranged by size: 6143 626 468 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5362 6227 6525 5362 Size: 865 298 6074 Fragments arranged by size: 6074 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2837 3476 3891 5900 882 Size: 617 1338 639 415 2009 2219 Fragments arranged by size: 2219 2009 1338 639 617 415 BsaAI yAC'GTr Cuts at: 493 1204 5028 493 Size: 711 3824 2702 Fragments arranged by size: 3824 2702 711 BsaBI GATnn'nnATC Cuts at: 7231 7231 Size: 7237 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3605 5044 5426 6061 Size: 53 83 186 936 2072 1439 382 635 Cuts at: 6061 275 Size: 1451 Fragments arranged by size: 2072 1451 1439 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3801 5670 6068 6342 6489 6900 3801 Size: 1869 398 274 147 411 4138 Fragments arranged by size: 4138 1869 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7012 Fragments arranged by size: 7012 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2956 3062 3202 3248 3530 4330 4333 1186 Size: 1770 106 140 46 282 800 3 4090 Fragments arranged by size: 4090 1770 800 282 140 106 46 3 BseYI C'CCAG_C Cuts at: 2689 2939 3161 3308 3315 3837 4296 4392 6440 Size: 250 222 147 7 522 459 96 2048 Cuts at: 6440 6936 2689 Size: 496 2990 Fragments arranged by size: 2990 2048 522 496 459 250 222 147 96 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4418 1163 Size: 573 2682 3982 Fragments arranged by size: 3982 2682 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4687 5448 6476 1540 Size: 275 186 2686 761 1028 2301 Fragments arranged by size: 2686 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2508 2863 3362 3702 5298 5383 6454 2508 Size: 355 499 340 1596 85 1071 3291 Fragments arranged by size: 3291 1596 1071 499 355 340 85 BsiWI C'GTAC_G Cuts at: 2485 2485 Size: 7237 BsmI GAATG_Cn' Cuts at: 2627 3410 4285 4748 4841 2627 Size: 783 875 463 93 5023 Fragments arranged by size: 5023 875 783 463 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2508 2782 2863 3054 3204 3248 3316 Size: 399 718 274 81 191 150 44 68 Cuts at: 3316 3362 3632 3702 3779 4250 4556 5298 5383 Size: 46 270 70 77 471 306 742 85 Cuts at: 5383 6454 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 718 471 399 306 274 270 191 150 85 81 77 70 68 46 44 BspEI T'CCGG_A Cuts at: 3801 6900 3801 Size: 3099 4138 BspHI T'CATG_A Cuts at: 2709 5126 6856 2709 Size: 2417 1730 3090 Fragments arranged by size: 3090 2417 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3909 4343 4592 844 Size: 3065 434 249 3489 Fragments arranged by size: 3489 3065 434 249 BsrBI CCG'CTC Cuts at: 1394 1823 5124 1394 Size: 429 3301 3507 Fragments arranged by size: 3507 3301 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2506 5732 5906 65 Size: 1183 1258 3226 174 1396 Fragments arranged by size: 3226 1396 1258 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3093 5881 6068 1700 Size: 110 1283 2788 187 2869 Fragments arranged by size: 2869 2788 1283 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7237 BssHII G'CGCG_C Cuts at: 1925 4400 1925 Size: 2475 4762 BssSI C'ACGA_G Cuts at: 5297 6309 5297 Size: 1012 6225 BstAPI GCAn_nnn'nTGC Cuts at: 794 2496 794 Size: 1702 5535 BstBI TT'CG_AA Cuts at: 6041 6041 Size: 7237 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7237 BstXI CCAn_nnnn'nTGG Cuts at: 2417 2417 Size: 7237 BstYI r'GATC_y Cuts at: 1972 2515 3437 3987 4567 4934 5319 5336 6788 Size: 543 922 550 580 367 385 17 1452 Cuts at: 6788 6874 6886 7232 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 922 580 550 543 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2347 2455 3633 3910 513 Size: 552 939 343 108 1178 277 3840 Fragments arranged by size: 3840 1178 939 552 343 277 108 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2413 2613 4437 487 Size: 683 56 1187 200 1824 3287 Fragments arranged by size: 3287 1824 1187 683 200 56 BtsI GCAGTG_nn' Cuts at: 2737 2863 2941 3019 3122 3560 4753 5547 5567 Size: 126 78 78 103 438 1193 794 20 Cuts at: 5567 2737 Size: 4407 Fragments arranged by size: 4407 1193 794 438 126 103 78 78 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7237 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2444 2479 529 Size: 35 1880 35 5287 Fragments arranged by size: 5287 1880 35 35 DraI TTT'AAA Cuts at: 4541 4541 Size: 7237 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6244 809 Size: 507 4928 1802 Fragments arranged by size: 4928 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2452 2695 2821 Size: 54 1425 50 275 18 622 243 126 Cuts at: 2821 2888 3369 4107 4240 4684 5573 7202 8 Size: 67 481 738 133 444 889 1629 43 Fragments arranged by size: 1629 1425 889 738 622 481 444 275 243 133 126 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4684 1537 Size: 275 2872 4090 Fragments arranged by size: 4090 2872 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2103 2172 2286 2291 3192 3516 3522 4012 Size: 750 69 114 5 901 324 6 490 Cuts at: 4012 4351 4508 5167 1353 Size: 339 157 659 3423 Fragments arranged by size: 3423 901 750 659 490 339 324 157 114 69 6 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2965 5824 6198 6344 387 Size: 1064 138 1376 2859 374 146 1280 Fragments arranged by size: 2859 1376 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2506 3360 2506 Size: 854 6383 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2156 2226 2327 2597 3205 3325 Size: 393 413 111 70 101 270 608 120 Cuts at: 3325 4332 5300 5897 6684 6952 1239 Size: 1007 968 597 787 268 1524 Fragments arranged by size: 1524 1007 968 787 608 597 413 393 270 268 120 111 101 70 EcoNI CCTnn'n_nnAGG Cuts at: 2285 2285 Size: 7237 EcoO109I rG'GnC_Cy Cuts at: 2122 3175 3312 4146 4221 4231 4301 2122 Size: 1053 137 834 75 10 70 5058 Fragments arranged by size: 5058 1053 834 137 75 70 10 EcoRI G'AATT_C Cuts at: 4546 4924 4546 Size: 378 6859 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4041 4041 Size: 7237 FspI TGC'GCA Cuts at: 5743 5743 Size: 7237 HaeII r_GCGC'y Cuts at: 1678 2704 3694 6384 1678 Size: 1026 990 2690 2531 Fragments arranged by size: 2690 2531 1026 990 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2075 2107 2397 2429 3104 Size: 32 135 32 503 32 290 32 675 Cuts at: 3104 3136 3480 3512 3553 3585 3689 3721 3890 Size: 32 344 32 41 32 104 32 169 Cuts at: 3890 3922 4371 4403 5878 5910 5952 5984 1373 Size: 32 449 32 1475 32 42 32 2626 Fragments arranged by size: 2626 1475 675 503 449 344 290 169 135 104 42 41 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2197 4581 4827 669 Size: 988 281 259 2384 246 3079 Fragments arranged by size: 3079 2384 988 281 259 246 HindIII A'AGCT_T Cuts at: 748 2309 748 Size: 1561 5676 HpaI GTT'AAC Cuts at: 4827 4827 Size: 7237 KpnI G_GTAC'C Cuts at: 4562 4562 Size: 7237 MfeI C'AATT_G Cuts at: 4836 4836 Size: 7237 MluI A'CGCG_T Cuts at: 7113 7113 Size: 7237 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3539 4075 4113 4242 6351 6535 1305 Size: 2234 536 38 129 2109 184 2007 Fragments arranged by size: 2234 2109 2007 536 184 129 38 MscI TGG'CCA Cuts at: 10 64 1489 1832 2454 3371 4109 10 Size: 54 1425 343 622 917 738 3138 Fragments arranged by size: 3138 1425 917 738 622 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2256 2432 2832 5195 5554 5713 518 Size: 648 1090 176 400 2363 359 159 2042 Fragments arranged by size: 2363 2042 1090 648 400 359 176 159 MspA1I CmG'CkG Cuts at: 1606 2023 2242 2349 3076 4192 4296 4653 5330 Size: 417 219 107 727 1116 104 357 677 Cuts at: 5330 6478 6723 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1116 727 677 417 357 245 219 107 104 NcoI C'CATG_G Cuts at: 513 1065 2004 3633 513 Size: 552 939 1629 4117 Fragments arranged by size: 4117 1629 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7237 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7237 NotI GC'GGCC_GC Cuts at: 4684 4684 Size: 7237 NsiI A_TGCA'T Cuts at: 3924 3924 Size: 7237 NspI r_CATG'y Cuts at: 1132 1229 2837 3139 3386 4595 6140 1132 Size: 97 1608 302 247 1209 1545 2229 Fragments arranged by size: 2229 1608 1545 1209 302 247 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7237 PciI A'CATG_T Cuts at: 6136 6136 Size: 7237 PflMI CCAn_nnn'nTGG Cuts at: 1108 3853 1108 Size: 2745 4492 PfoI T'CCnGG_A Cuts at: 2203 3292 4200 4223 2203 Size: 1089 908 23 5217 Fragments arranged by size: 5217 1089 908 23 PmeI GTTT'AAAC Cuts at: 4541 4541 Size: 7237 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2063 2095 5306 5338 6819 6851 2063 Size: 32 3211 32 1481 32 2449 Fragments arranged by size: 3211 2449 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2122 3175 4221 4231 4301 2122 Size: 1053 1046 10 70 5058 Fragments arranged by size: 5058 1053 1046 70 10 PshAI GACnn'nnGTC Cuts at: 1613 2802 1613 Size: 1189 6048 PsiI TTA'TAA Cuts at: 4807 4807 Size: 7237 PspOMI G'GGCC_C Cuts at: 3312 3312 Size: 7237 PstI C_TGCA'G Cuts at: 830 2936 3129 3255 3906 4589 830 Size: 2106 193 126 651 683 3478 Fragments arranged by size: 3478 2106 683 651 193 126 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7237 PvuII CAG'CTG Cuts at: 2242 3076 2242 Size: 834 6403 SacI G_AGCT'C Cuts at: 2508 3362 2508 Size: 854 6383 SacII CC_GC'GG Cuts at: 2350 2350 Size: 7237 SalI G'TCGA_C Cuts at: 1936 4579 1936 Size: 2643 4594 SanDI GG'GwC_CC Cuts at: 2122 3175 4221 4301 2122 Size: 1053 1046 80 5058 Fragments arranged by size: 5058 1053 1046 80 SapI GCTCTTCn'nnn_ Cuts at: 3192 4012 4508 3192 Size: 820 496 5921 Fragments arranged by size: 5921 820 496 SbfI CC_TGCA'GG Cuts at: 4589 4589 Size: 7237 ScaI AGT'ACT Cuts at: 2159 5485 7197 2159 Size: 3326 1712 2199 Fragments arranged by size: 3326 2199 1712 SfcI C'TryA_G Cuts at: 826 1045 2161 2932 3125 3251 3286 3451 3892 Size: 219 1116 771 193 126 35 165 441 Cuts at: 3892 3902 4585 5720 6401 6592 826 Size: 10 683 1135 681 191 1471 Fragments arranged by size: 1471 1135 1116 771 683 681 441 219 193 191 165 126 35 10 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7237 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7237 SmaI CCC'GGG Cuts at: 1872 3426 4564 1872 Size: 1554 1138 4545 Fragments arranged by size: 4545 1554 1138 SmlI C'TyrA_G Cuts at: 820 1017 1943 5341 6242 6504 820 Size: 197 926 3398 901 262 1553 Fragments arranged by size: 3398 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5028 493 Size: 4535 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7237 SphI G_CATG'C Cuts at: 1132 2837 4595 1132 Size: 1705 1758 3774 Fragments arranged by size: 3774 1758 1705 SspI AAT'ATT Cuts at: 5 52 2765 5161 5 Size: 47 2713 2396 2081 Fragments arranged by size: 2713 2396 2081 47 StuI AGG'CCT Cuts at: 1517 2362 3666 1517 Size: 845 1304 5088 Fragments arranged by size: 5088 1304 845 StyI C'CwwG_G Cuts at: 513 1065 2004 2800 2997 3633 4250 4375 4676 Size: 552 939 796 197 636 617 125 301 Cuts at: 4676 513 Size: 3074 Fragments arranged by size: 3074 939 796 636 617 552 301 197 125 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3175 5264 5281 5434 5619 6064 325 Size: 1255 1595 2089 17 153 185 445 1498 Fragments arranged by size: 2089 1595 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2157 5483 7195 Size: 274 80 33 51 1443 180 3326 1712 Cuts at: 7195 96 Size: 138 Fragments arranged by size: 3326 1712 1443 274 180 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3735 3767 7197 7229 3735 Size: 32 3430 32 3743 Fragments arranged by size: 3743 3430 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4258 1316 Size: 2942 4295 XbaI T'CTAG_A Cuts at: 4573 4573 Size: 7237 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2417 3640 2417 Size: 1223 6014 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7237 XmaI C'CCGG_G Cuts at: 1870 3424 4562 1870 Size: 1554 1138 4545 Fragments arranged by size: 4545 1554 1138 XmnI GAAnn'nnTTC Cuts at: 2312 5366 2312 Size: 3054 4183 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5045 276 Size: 53 83 186 936 3511 2468 Fragments arranged by size: 3511 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BbeI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SexAI SfiI SfoI SrfI SwaI