Restriction map for
pFN21AE0323
(Linear) (Six-base) MAPSORT of: KIEE0323.seq Check: 6367 from: 1 to: 2532
KIEE0323.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2338 2532
Size: 2338 194
AccI GT'mk_AC
Cuts at: 0 1106 2532
Size: 1106 1426
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 322 592 1320 2327 2532
Size: 151 171 270 728 1007 205
Fragments arranged by size:
1007 728 270 205 171 151
AfeI AGC'GCT
Cuts at: 0 697 2532
Size: 697 1835
AhdI GACnn_n'nnGTC
Cuts at: 0 1113 1899 2532
Size: 1113 786 633
Fragments arranged by size:
1113 786 633
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 633 1975 2532
Size: 633 1342 557
Fragments arranged by size:
1342 633 557
AlwNI CAG_nnn'CTG
Cuts at: 0 628 2350 2532
Size: 628 1722 182
Fragments arranged by size:
1722 628 182
ApaI G_GGCC'C
Cuts at: 0 1311 2532
Size: 1311 1221
AvaI C'yCGr_G
Cuts at: 0 1419 1481 1619 2532
Size: 1419 62 138 913
Fragments arranged by size:
1419 913 138 62
AvrII C'CTAG_G
Cuts at: 0 2245 2370 2532
Size: 2245 125 162
Fragments arranged by size:
2245 162 125
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 136 169 2532
Size: 136 33 2363
Fragments arranged by size:
2363 136 33
BamHI G'GATC_C
Cuts at: 0 1982 2532
Size: 1982 550
BanI G'GyrC_C
Cuts at: 0 464 554 1678 2532
Size: 464 90 1124 854
Fragments arranged by size:
1124 854 464 90
BanII G_rGCy'C
Cuts at: 0 503 1049 1199 1243 1311 1357 1627 2532
Size: 503 546 150 44 68 46 270 905
Fragments arranged by size:
905 546 503 270 150 68 46 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 37 723 947 1415 2532
Size: 37 686 224 468 1117
Fragments arranged by size:
1117 686 468 224 37
BbvCI CC'TCA_GC
Cuts at: 0 629 1255 1723 2532
Size: 629 626 468 809
Fragments arranged by size:
809 629 626 468
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1600 1634 2532
Size: 1600 34 898
Fragments arranged by size:
1600 898 34
BfrBI ATG'CAT
Cuts at: 0 1917 2532
Size: 1917 615
BglII A'GATC_T
Cuts at: 0 1432 2532
Size: 1432 1100
BlpI GC'TnA_GC
Cuts at: 0 1135 1759 1870 2532
Size: 1135 624 111 662
Fragments arranged by size:
1135 662 624 111
Bme1580I G_kGCm'C
Cuts at: 0 777 1311 1774 2245 2532
Size: 777 534 463 471 287
Fragments arranged by size:
777 534 471 463 287
BmrI ACTGGGnnnn_n'
Cuts at: 0 358 2532
Size: 358 2174
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2139 2532
Size: 2139 393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 221 1200 2532
Size: 40 181 979 1332
Fragments arranged by size:
1332 979 181 40
Bpu10I CC'TnA_GC
Cuts at: 0 629 1255 1723 2532
Size: 629 626 468 809
Fragments arranged by size:
809 629 626 468
BsaI GGTCTCn'nnnn_
Cuts at: 0 832 1471 1886 2532
Size: 832 639 415 646
Fragments arranged by size:
832 646 639 415
BsaHI Gr'CG_yC
Cuts at: 0 1600 2532
Size: 1600 932
BsaWI w'CCGG_w
Cuts at: 0 1796 2532
Size: 1796 736
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 951 1057 1197 1243 1525 2325 2328 2532
Size: 951 106 140 46 282 800 3 204
Fragments arranged by size:
951 800 282 204 140 106 46 3
BseYI C'CCAG_C
Cuts at: 0 684 934 1156 1303 1310 1832 2291 2387
Size: 684 250 222 147 7 522 459 96
Cuts at: 2387 2532
Size: 145
Fragments arranged by size:
684 522 459 250 222 147 145 96
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2413 2532
Size: 2413 119
BsiHKAI G_wGCw'C
Cuts at: 0 503 858 1357 1697 2532
Size: 503 355 499 340 835
Fragments arranged by size:
835 503 499 355 340
BsiWI C'GTAC_G
Cuts at: 0 480 2532
Size: 480 2052
BsmI GAATG_Cn'
Cuts at: 0 622 1405 2280 2532
Size: 622 783 875 252
Fragments arranged by size:
875 783 622 252
Bsp1286I G_dGCh'C
Cuts at: 0 503 777 858 1049 1199 1243 1311 1357
Size: 503 274 81 191 150 44 68 46
Cuts at: 1357 1627 1697 1774 2245 2532
Size: 270 70 77 471 287
Fragments arranged by size:
503 471 287 274 270 191 150 81
77 70 68 46 44
BspEI T'CCGG_A
Cuts at: 0 1796 2532
Size: 1796 736
BspHI T'CATG_A
Cuts at: 0 704 2532
Size: 704 1828
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1904 2338 2532
Size: 1904 434 194
Fragments arranged by size:
1904 434 194
BsrDI GCAATG_nn'
Cuts at: 0 501 2532
Size: 501 2031
BsrFI r'CCGG_y
Cuts at: 0 1088 2532
Size: 1088 1444
BssHII G'CGCG_C
Cuts at: 0 2395 2532
Size: 2395 137
BstAPI GCAn_nnn'nTGC
Cuts at: 0 491 2532
Size: 491 2041
BstXI CCAn_nnnn'nTGG
Cuts at: 0 412 2532
Size: 412 2120
BstYI r'GATC_y
Cuts at: 0 510 1432 1982 2532
Size: 510 922 550 550
Fragments arranged by size:
922 550 550 510
BtgI C'CryG_G
Cuts at: 0 342 450 1628 1905 2532
Size: 342 108 1178 277 627
Fragments arranged by size:
1178 627 342 277 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 408 608 2432 2532
Size: 408 200 1824 100
Fragments arranged by size:
1824 408 200 100
BtsI GCAGTG_nn'
Cuts at: 0 732 858 936 1014 1117 1555 2532
Size: 732 126 78 78 103 438 977
Fragments arranged by size:
977 732 438 126 103 78 78
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 439 474 2532
Size: 439 35 2058
Fragments arranged by size:
2058 439 35
EaeI y'GGCC_r
Cuts at: 0 447 690 816 883 1364 2102 2235 2532
Size: 447 243 126 67 481 738 133 297
Fragments arranged by size:
738 481 447 297 243 133 126 67
EarI CTCTTCn'nnn_
Cuts at: 0 98 167 281 286 1187 1511 1517 2007
Size: 98 69 114 5 901 324 6 490
Cuts at: 2007 2346 2503 2532
Size: 339 157 29
Fragments arranged by size:
901 490 339 324 157 114 98 69
29 6 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 960 2532
Size: 960 1572
EcoICRI GAG'CTC
Cuts at: 0 501 1355 2532
Size: 501 854 1177
Fragments arranged by size:
1177 854 501
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 151 221 322 592 1200 1320 2327
Size: 40 111 70 101 270 608 120 1007
Cuts at: 2327 2532
Size: 205
Fragments arranged by size:
1007 608 270 205 120 111 101 70
40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 280 2532
Size: 280 2252
EcoO109I rG'GnC_Cy
Cuts at: 0 117 1170 1307 2141 2216 2226 2296 2532
Size: 117 1053 137 834 75 10 70 236
Fragments arranged by size:
1053 834 236 137 117 75 70 10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2036 2532
Size: 2036 496
HaeII r_GCGC'y
Cuts at: 0 699 1689 2532
Size: 699 990 843
Fragments arranged by size:
990 843 699
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 392 424 1099 1131 1475 1507
Size: 70 32 290 32 675 32 344 32
Cuts at: 1507 1548 1580 1684 1716 1885 1917 2366 2398
Size: 41 32 104 32 169 32 449 32
Cuts at: 2398 2532
Size: 134
Fragments arranged by size:
675 449 344 290 169 134 104 70
41 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 192 2532
Size: 192 2340
HindIII A'AGCT_T
Cuts at: 0 304 2532
Size: 304 2228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1534 2070 2108 2237 2532
Size: 1534 536 38 129 295
Fragments arranged by size:
1534 536 295 129 38
MscI TGG'CCA
Cuts at: 0 449 1366 2104 2532
Size: 449 917 738 428
Fragments arranged by size:
917 738 449 428
MslI CAynn'nnrTG
Cuts at: 0 251 427 827 2532
Size: 251 176 400 1705
Fragments arranged by size:
1705 400 251 176
MspA1I CmG'CkG
Cuts at: 0 18 237 344 1071 2187 2291 2532
Size: 18 219 107 727 1116 104 241
Fragments arranged by size:
1116 727 241 219 107 104 18
NcoI C'CATG_G
Cuts at: 0 1628 2532
Size: 1628 904
NsiI A_TGCA'T
Cuts at: 0 1919 2532
Size: 1919 613
NspI r_CATG'y
Cuts at: 0 832 1134 1381 2532
Size: 832 302 247 1151
Fragments arranged by size:
1151 832 302 247
PflMI CCAn_nnn'nTGG
Cuts at: 0 1848 2532
Size: 1848 684
PfoI T'CCnGG_A
Cuts at: 0 198 1287 2195 2218 2532
Size: 198 1089 908 23 314
Fragments arranged by size:
1089 908 314 198 23
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 2532
Size: 58 32 2442
Fragments arranged by size:
2442 58 32
PpuMI rG'GwC_Cy
Cuts at: 0 117 1170 2216 2226 2296 2532
Size: 117 1053 1046 10 70 236
Fragments arranged by size:
1053 1046 236 117 70 10
PshAI GACnn'nnGTC
Cuts at: 0 797 2532
Size: 797 1735
PspOMI G'GGCC_C
Cuts at: 0 1307 2532
Size: 1307 1225
PstI C_TGCA'G
Cuts at: 0 931 1124 1250 1901 2532
Size: 931 193 126 651 631
Fragments arranged by size:
931 651 631 193 126
PvuII CAG'CTG
Cuts at: 0 237 1071 2532
Size: 237 834 1461
Fragments arranged by size:
1461 834 237
SacI G_AGCT'C
Cuts at: 0 503 1357 2532
Size: 503 854 1175
Fragments arranged by size:
1175 854 503
SacII CC_GC'GG
Cuts at: 0 345 2532
Size: 345 2187
SanDI GG'GwC_CC
Cuts at: 0 117 1170 2216 2296 2532
Size: 117 1053 1046 80 236
Fragments arranged by size:
1053 1046 236 117 80
SapI GCTCTTCn'nnn_
Cuts at: 0 1187 2007 2503 2532
Size: 1187 820 496 29
Fragments arranged by size:
1187 820 496 29
ScaI AGT'ACT
Cuts at: 0 154 2532
Size: 154 2378
SfcI C'TryA_G
Cuts at: 0 156 927 1120 1246 1281 1446 1887 1897
Size: 156 771 193 126 35 165 441 10
Cuts at: 1897 2532
Size: 635
Fragments arranged by size:
771 635 441 193 165 156 126 35
10
SmaI CCC'GGG
Cuts at: 0 1421 2532
Size: 1421 1111
SphI G_CATG'C
Cuts at: 0 832 2532
Size: 832 1700
SspI AAT'ATT
Cuts at: 0 760 2532
Size: 760 1772
StuI AGG'CCT
Cuts at: 0 357 1661 2532
Size: 357 1304 871
Fragments arranged by size:
1304 871 357
StyI C'CwwG_G
Cuts at: 0 795 992 1628 2245 2370 2532
Size: 795 197 636 617 125 162
Fragments arranged by size:
795 636 617 197 162 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1170 2532
Size: 1170 1362
TatI w'GTAC_w
Cuts at: 0 152 2532
Size: 152 2380
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1730 1762 2532
Size: 1730 32 770
Fragments arranged by size:
1730 770 32
Tth111I GACn'n_nGTC
Cuts at: 0 2253 2532
Size: 2253 279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 412 1635 2532
Size: 412 1223 897
Fragments arranged by size:
1223 897 412
XmaI C'CCGG_G
Cuts at: 0 1419 2532
Size: 1419 1113
XmnI GAAnn'nnTTC
Cuts at: 0 307 2532
Size: 307 2225
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AlfI AlwNI ApaI
AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI
BcgI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BsaI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI
BssHII BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I
NcoI NsiI NspI PflMI PfoI PpiI PpuMI PshAI
PspOMI PstI PvuII SacI SacII SanDI SapI ScaI
SfcI SmaI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AleI AloI
ApaLI ApoI AscI AseI AsiSI BbeI BciVI BclI
BglI BmgBI BmtI BpuEI BsaAI BsaBI BsaXI BsiEI
BsmBI BsrBI BsrGI BssSI BstBI BstEII BstZ17I Bsu36I
ClaI DraI DraIII DrdI EagI EcoRI EcoRV FseI
FspI FspAI HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PmeI PmlI PsiI PspXI PsrI PvuI RsrII
SalI SbfI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI SwaI XbaI XhoI ZraI