################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HpaI KpnI KspI MaeI MamI MluI MslI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tsp45I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccIII 535 TCCGGA T^CCGGA AflIII 338 ACACGT A^C[GA][CT]GT AspHI 777 GTGCTC G[AT]GC[AT]^C AvaI 195 CCCGGG C^[CT]CG[GA]G BalI 174 TGGCCA TGG^CCA BamHI 507 GGATCC G^GATCC BpmI 744 CTGGAG CTGGAG(16/14) BsaI 979 GGTCTC GGTCTC(1/5) BseAI 535 TCCGGA T^CCGGA BsiHKAI 777 GTGCTC G[AT]GC[AT]^C BsmFI 362 GTCCC GGGAC(10/14) BsmI 765 GAATGC GAATGC(1/-1) BsoBI 195 CCCGGG C^[CT]CG[GA]G BspEI 535 TCCGGA T^CCGGA BspMI 704 ACCTGC ACCTGC(4/8) BsrDI 4 GCAATG GCAATG(2/0) BssHII 558 GCGCGC G^CGCGC DraII 137 GGGGCCC [GA]G^G[ACGT]CC[CT] DrdI 847 GACCGGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 480 CCACGG C^C[GA][CT]GG EagI 167 CGGCCG C^GGCCG EarI 658 CTCTTC CTCTTC(1/4) EclXI 167 CGGCCG C^GGCCG Eco52I 167 CGGCCG C^GGCCG EcoO109I 137 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoT14I 132 CCAAGG C^C[AT][AT]GG EcoT22I 597 ATGCAT ATGCA^T HgaI 123 GACGC GACGC(5/10) HincII 919 GTTGAC GT[CT]^[GA]AC HindII 919 GTTGAC GT[CT]^[GA]AC Ksp632I 658 CTCTTC CTCTTC(1/4) MaeIII 1197 GTTAC ^GT[ACGT]AC MfeI 1138 CAATTG C^AATTG MluNI 174 TGGCCA TGG^CCA MroI 535 TCCGGA T^CCGGA MscI 174 TGGCCA TGG^CCA MseI 1217 TTAA T^TAA MspA1I 936 CAGCTG C[AC]G^C[GT]G MunI 1138 CAATTG C^AATTG NaeI 817 GCCGGC GCC^GGC NgoMIV 817 GCCGGC G^CCGGC NsiI 597 ATGCAT ATGCA^T PleI 922 GACTC GAGTC(4/5) PstI 706 CTGCAG CTGCA^G PvuII 936 CAGCTG CAG^CTG SapI 657 GCTCTTC GCTCTTC(1/4) ScaI 395 AGTACT AGT^ACT SexAI 719 ACCAGGT A^CC[AT]GGT SfcI 706 CTGCAG C^T[GA][CT]AG SmaI 195 CCCGGG CCC^GGG StuI 65 AGGCCT AGG^CCT StyI 132 CCAAGG C^C[AT][AT]GG TfiI 1076 GATTC G^A[AT]TC Tru9I 1217 TTAA T^TAA XmaCI 195 CCCGGG C^CCGGG XmaI 195 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### ApaI 138 GGGCCC GGGCC^C 677 GGGCCC AvaII 717 GGACC G^G[AT]CC 867 GGACC BbeI 75 GGCGCC GGCGC^C 103 GGCGCC BsaWI 353 ACCGGA [AT]^CCGG[AT] 535 TCCGGA BseRI 1119 CTCCTC GAGGAG(10/8) 1122 CTCCTC BsgI 913 CTGCAC GTGCAG(16/14) 445 GTGCAG BsiEI 167 CGGCCG CG[GA][CT]^CG 846 CGACCG BsmBI 1116 CGTCTC CGTCTC(1/5) 432 GAGACG BsrBI 802 CCGCTC CCGCTC(-3/-3) 258 GAGCGG BsrFI 169 GCCGGT [GA]^CCGG[CT] 817 GCCGGC BstAPI 383 GCAACCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 590 GCAGCCAATGC BstYI 507 GGATCC [GA]^GATC[CT] 878 GGATCT Cfr10I 169 GCCGGT [GA]^CCGG[CT] 817 GCCGGC HindIII 1005 AAGCTT A^AGCTT 1084 AAGCTT HinfI 922 GACTC G^A[ACGT]TC 1076 GATTC HphI 740 TCACC GGTGA(8/7) 962 TCACC KasI 75 GGCGCC G^GCGCC 103 GGCGCC MflI 507 GGATCC [GA]^GATC[CT] 878 GGATCT NarI 75 GGCGCC GG^CGCC 103 GGCGCC NspI 599 GCATGC [GA]CATG^[CT] 825 GCATGC SfoI 75 GGCGCC GGC^GCC 103 GGCGCC SmlI 904 CTCAAG C^T[CT][GA]AG 943 CTCAAG SphI 599 GCATGC GCATG^C 825 GCATGC Tsp509I 1139 AATT ^AATT 1236 AATT XcmI 177 CCAGGGCGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 608 CCAACAACATCATGG XhoII 507 GGATCC [GA]^GATC[CT] 878 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI BanI BanII BbrPI BbvI BcnI BglI BmyI BsaAI BsaHI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MaeII MboI MboII MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PmaCI PmlI RsaI Sau3AI Sau96I ScrFI SfaNI SfiI TaqI TseI TspRI TthHB8I