(Circular) (Six-base) MAPSORT of: pFN21AB7696.seq Check: 1068 from: 1 to: 5947 pFN21AB7696 5947 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3757 278 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3290 1937 Size: 1353 4594 Acc65I G'GTAC_C Cuts at: 3268 3268 Size: 5947 AclI AA'CG_TT Cuts at: 1563 4074 4447 1563 Size: 2511 373 3063 Fragments arranged by size: 3063 2511 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2650 4010 5394 5662 1632 Size: 1018 1360 1384 268 1917 Fragments arranged by size: 1917 1384 1360 1018 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5750 AflIII A'CryG_T Cuts at: 1203 2343 4846 5823 1203 Size: 1140 2503 977 1327 Fragments arranged by size: 2503 1327 1140 977 AgeI A'CCGG_T Cuts at: 4778 4778 Size: 5947 AhdI GACnn_n'nnGTC Cuts at: 4676 4676 Size: 5947 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5947 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5947 AlwNI CAG_nnn'CTG Cuts at: 3690 5262 3690 Size: 1572 4375 ApaI G_GGCC'C Cuts at: 2147 2686 2147 Size: 539 5408 ApaLI G'TGCA_C Cuts at: 4004 5160 4004 Size: 1156 4791 ApoI r'AATT_y Cuts at: 1493 3256 3482 3634 5743 1493 Size: 1763 226 152 2109 1697 Fragments arranged by size: 2109 1763 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4501 160 Size: 872 3469 1606 Fragments arranged by size: 3469 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 AvaI C'yCGr_G Cuts at: 1870 1943 2200 3272 1870 Size: 73 257 1072 4545 Fragments arranged by size: 4545 1072 257 73 BamHI G'GATC_C Cuts at: 2512 3277 3644 2512 Size: 765 367 4815 Fragments arranged by size: 4815 765 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2047 2080 2108 3268 4723 Size: 325 217 627 260 33 28 1160 1455 Cuts at: 4723 618 Size: 1842 Fragments arranged by size: 1842 1455 1160 627 325 260 217 33 28 BanII G_rGCy'C Cuts at: 1391 2147 2574 2686 3266 1391 Size: 756 427 112 580 4072 Fragments arranged by size: 4072 756 580 427 112 BbeI G_GCGC'C Cuts at: 2084 2112 2084 Size: 28 5919 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4976 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4138 4172 1903 Size: 34 2201 34 3678 Fragments arranged by size: 3678 2201 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3839 5049 3839 Size: 1210 4737 BclI T'GATC_A Cuts at: 1548 1548 Size: 5947 BfrBI ATG'CAT Cuts at: 2604 2604 Size: 5947 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 2142 2168 Size: 107 122 71 631 605 406 64 26 Cuts at: 2168 2187 2196 4558 136 Size: 19 9 2362 1525 Fragments arranged by size: 2362 1525 631 605 406 122 107 71 64 26 19 9 BglII A'GATC_T Cuts at: 5942 5942 Size: 5947 BlpI GC'TnA_GC Cuts at: 3364 3364 Size: 5947 Bme1580I G_kGCm'C Cuts at: 1790 2052 2094 2147 2686 4008 5164 1790 Size: 262 42 53 539 1322 1156 2573 Fragments arranged by size: 2573 1322 1156 539 262 53 42 BmgBI CAC'GTC Cuts at: 1318 2583 2800 1318 Size: 1265 217 4465 Fragments arranged by size: 4465 1265 217 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3678 4636 442 Size: 416 53 83 399 24 2261 958 1753 Fragments arranged by size: 2261 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5947 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2770 4607 1239 Size: 1531 1837 2579 Fragments arranged by size: 2579 1837 1531 Bpu10I CC'TnA_GC Cuts at: 2336 2336 Size: 5947 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2894 2933 4072 4937 5235 2894 Size: 39 1139 865 298 3606 Fragments arranged by size: 3606 1139 865 298 39 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2990 4610 882 Size: 617 1491 1620 2219 Fragments arranged by size: 2219 1620 1491 617 BsaAI yAC'GTr Cuts at: 493 1204 2025 2289 2346 3738 493 Size: 711 821 264 57 1392 2702 Fragments arranged by size: 2702 1392 821 711 264 57 BsaBI GATnn'nnATC Cuts at: 5941 5941 Size: 5947 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2081 2109 2129 3754 Size: 53 83 186 936 548 28 20 1625 Cuts at: 3754 4136 4771 275 Size: 382 635 1451 Fragments arranged by size: 1625 1451 936 635 548 382 186 83 53 28 20 BsaWI w'CCGG_w Cuts at: 2358 2540 4380 4778 5052 5199 5610 2358 Size: 182 1840 398 274 147 411 2695 Fragments arranged by size: 2695 1840 411 398 274 182 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5722 Fragments arranged by size: 5722 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3115 3118 1186 Size: 1929 3 4015 Fragments arranged by size: 4015 1929 3 BseYI C'CCAG_C Cuts at: 2146 2475 5150 5646 2146 Size: 329 2675 496 2447 Fragments arranged by size: 2675 2447 496 329 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2471 2903 1163 Size: 573 735 432 4207 Fragments arranged by size: 4207 735 573 432 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2175 2854 3397 4158 5186 1540 Size: 275 186 174 679 543 761 1028 2301 Fragments arranged by size: 2301 1028 761 679 543 275 186 174 BsiHKAI G_wGCw'C Cuts at: 2786 4008 4093 5164 2786 Size: 1222 85 1071 3569 Fragments arranged by size: 3569 1222 1071 85 BsmI GAATG_Cn' Cuts at: 2776 3458 3551 2776 Size: 682 93 5172 Fragments arranged by size: 5172 682 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2431 3127 2431 Size: 696 5251 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2094 2147 2574 2686 2786 3266 Size: 399 262 42 53 427 112 100 480 Cuts at: 3266 4008 4093 5164 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 480 427 399 262 112 100 85 53 42 BspEI T'CCGG_A Cuts at: 2540 5610 2540 Size: 3070 2877 BspHI T'CATG_A Cuts at: 3836 5566 3836 Size: 1730 4217 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2718 3302 844 Size: 1874 584 3489 Fragments arranged by size: 3489 1874 584 BsrBI CCG'CTC Cuts at: 1394 1823 2265 2809 3834 1394 Size: 429 442 544 1025 3507 Fragments arranged by size: 3507 1025 544 442 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2016 4442 4616 65 Size: 1183 768 2426 174 1396 Fragments arranged by size: 2426 1396 1183 768 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2174 2822 4591 4778 1700 Size: 110 364 648 1769 187 2869 Fragments arranged by size: 2869 1769 648 364 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5947 BssHII G'CGCG_C Cuts at: 1925 2563 1925 Size: 638 5309 BssSI C'ACGA_G Cuts at: 4007 5019 4007 Size: 1012 4935 BstAPI GCAn_nnn'nTGC Cuts at: 794 2394 2601 794 Size: 1600 207 4140 Fragments arranged by size: 4140 1600 207 BstBI TT'CG_AA Cuts at: 4751 4751 Size: 5947 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5947 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5947 BstYI r'GATC_y Cuts at: 1972 2512 2883 3277 3644 4029 4046 5498 5584 Size: 540 371 394 367 385 17 1452 86 Cuts at: 5584 5596 5942 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 540 394 385 371 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2485 513 Size: 552 939 481 3975 Fragments arranged by size: 3975 939 552 481 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2369 487 Size: 683 56 1143 4065 Fragments arranged by size: 4065 1143 683 56 BtsI GCAGTG_nn' Cuts at: 2786 3463 4257 4277 2786 Size: 677 794 20 4456 Fragments arranged by size: 4456 794 677 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5947 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5912 DraI TTT'AAA Cuts at: 3251 3251 Size: 5947 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2858 4954 809 Size: 507 1542 2096 1802 Fragments arranged by size: 2096 1802 1542 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2134 2172 2179 Size: 54 1425 50 275 18 304 38 7 Cuts at: 2179 2278 2649 2814 3227 3394 4283 5912 8 Size: 99 371 165 413 167 889 1629 43 Fragments arranged by size: 1629 1425 889 413 371 304 275 167 165 99 54 50 43 38 18 7 EagI C'GGCC_G Cuts at: 1537 1812 2172 3394 1537 Size: 275 360 1222 4090 Fragments arranged by size: 4090 1222 360 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2669 3877 1353 Size: 1316 1208 3423 Fragments arranged by size: 3423 1316 1208 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2836 4534 4908 5054 387 Size: 1064 138 1247 1698 374 146 1280 Fragments arranged by size: 1698 1280 1247 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2650 2770 4010 4607 5394 5662 1239 Size: 393 1018 120 1240 597 787 268 1524 Fragments arranged by size: 1524 1240 1018 787 597 393 268 120 EcoO109I rG'GnC_Cy Cuts at: 2143 2143 Size: 5947 EcoRI G'AATT_C Cuts at: 3256 3634 3256 Size: 378 5569 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2326 2326 Size: 5947 FspI TGC'GCA Cuts at: 4453 4453 Size: 5947 HaeII r_GCGC'y Cuts at: 1678 2084 2112 2673 2918 5094 1678 Size: 406 28 561 245 2176 2531 Fragments arranged by size: 2531 2176 561 406 245 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4588 4620 4662 4694 1373 Size: 32 135 32 3016 32 42 32 2626 Fragments arranged by size: 3016 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2926 3291 3537 669 Size: 988 281 988 365 246 3079 Fragments arranged by size: 3079 988 988 365 281 246 HindIII A'AGCT_T Cuts at: 748 3010 3089 748 Size: 2262 79 3606 Fragments arranged by size: 3606 2262 79 HpaI GTT'AAC Cuts at: 3537 3537 Size: 5947 KasI G'GCGC_C Cuts at: 2080 2108 2080 Size: 28 5919 KpnI G_GTAC'C Cuts at: 3272 3272 Size: 5947 MfeI C'AATT_G Cuts at: 3143 3546 3143 Size: 403 5544 MluI A'CGCG_T Cuts at: 5823 5823 Size: 5947 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5061 5245 1305 Size: 3756 184 2007 Fragments arranged by size: 3756 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2181 10 Size: 54 1425 343 349 3776 Fragments arranged by size: 3776 1425 349 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3905 4264 4423 518 Size: 648 843 1896 359 159 2042 Fragments arranged by size: 2042 1896 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2943 3363 4040 5188 5433 1606 Size: 1337 420 677 1148 245 2120 Fragments arranged by size: 2120 1337 1148 677 420 245 NaeI GCC'GGC Cuts at: 2824 2824 Size: 5947 NarI GG'CG_CC Cuts at: 2081 2109 2081 Size: 28 5919 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4456 Fragments arranged by size: 4456 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5947 NgoMIV G'CCGG_C Cuts at: 2822 2822 Size: 5947 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5947 NotI GC'GGCC_GC Cuts at: 3394 3394 Size: 5947 NsiI A_TGCA'T Cuts at: 2606 2606 Size: 5947 NspI r_CATG'y Cuts at: 1132 1229 2608 2834 3305 4850 1132 Size: 97 1379 226 471 1545 2229 Fragments arranged by size: 2229 1545 1379 471 226 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5947 PciI A'CATG_T Cuts at: 4846 4846 Size: 5947 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5947 PfoI T'CCnGG_A Cuts at: 3192 3192 Size: 5947 PmeI GTTT'AAAC Cuts at: 3251 3251 Size: 5947 PmlI CAC'GTG Cuts at: 2025 2289 2346 2025 Size: 264 57 5626 Fragments arranged by size: 5626 264 57 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4016 4048 5529 5561 4016 Size: 32 1481 32 4402 Fragments arranged by size: 4402 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5947 PsiI TTA'TAA Cuts at: 3517 3517 Size: 5947 PspOMI G'GGCC_C Cuts at: 2143 2682 2143 Size: 539 5408 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2324 2356 2324 Size: 32 5915 PstI C_TGCA'G Cuts at: 830 2715 3299 830 Size: 1885 584 3478 Fragments arranged by size: 3478 1885 584 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5947 PvuII CAG'CTG Cuts at: 2943 2943 Size: 5947 SalI G'TCGA_C Cuts at: 1936 3289 1936 Size: 1353 4594 SapI GCTCTTCn'nnn_ Cuts at: 2669 2669 Size: 5947 SbfI CC_TGCA'GG Cuts at: 3299 3299 Size: 5947 ScaI AGT'ACT Cuts at: 2402 4195 5907 2402 Size: 1793 1712 2442 Fragments arranged by size: 2442 1793 1712 SexAI A'CCwGG_T Cuts at: 2724 2724 Size: 5947 SfcI C'TryA_G Cuts at: 826 1045 2711 3295 4430 5111 5302 826 Size: 219 1666 584 1135 681 191 1471 Fragments arranged by size: 1666 1471 1135 681 584 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2142 2187 2196 2142 Size: 45 9 5893 Fragments arranged by size: 5893 45 9 SfoI GGC'GCC Cuts at: 2082 2110 2082 Size: 28 5919 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5947 SmaI CCC'GGG Cuts at: 1872 2202 3274 1872 Size: 330 1072 4545 Fragments arranged by size: 4545 1072 330 SmlI C'TyrA_G Cuts at: 820 1017 1943 2909 2948 4051 4952 5214 820 Size: 197 926 966 39 1103 901 262 1553 Fragments arranged by size: 1553 1103 966 926 901 262 197 39 SnaBI TAC'GTA Cuts at: 493 3738 493 Size: 3245 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5947 SphI G_CATG'C Cuts at: 1132 2608 2834 3305 1132 Size: 1476 226 471 3774 Fragments arranged by size: 3774 1476 471 226 SspI AAT'ATT Cuts at: 5 52 3871 5 Size: 47 3819 2081 Fragments arranged by size: 3819 2081 47 StuI AGG'CCT Cuts at: 1517 2072 1517 Size: 555 5392 StyI C'CwwG_G Cuts at: 513 1065 2004 2137 3386 513 Size: 552 939 133 1249 3074 Fragments arranged by size: 3074 1249 939 552 133 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3974 3991 4144 4329 4774 325 Size: 1255 2394 17 153 185 445 1498 Fragments arranged by size: 2394 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2400 2937 4193 Size: 274 80 33 51 1443 423 537 1256 Cuts at: 4193 5905 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1256 537 423 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5907 5939 5907 Size: 32 5915 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5947 XbaI T'CTAG_A Cuts at: 3283 3283 Size: 5947 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2189 2620 2189 Size: 431 5516 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5947 XmaI C'CCGG_G Cuts at: 1870 2200 3272 1870 Size: 330 1072 4545 Fragments arranged by size: 4545 1072 330 XmnI GAAnn'nnTTC Cuts at: 4076 4076 Size: 5947 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3755 276 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI PacI PpuMI PspXI RsrII SacI SacII SanDI SrfI SwaI