(Linear) (Six-base) MAPSORT of: KIBB7696.seq Check: 5396 from: 1 to: 1242 KIBB7696.orf With 182 enzymes: SgfI * July 15, 2010 18:31 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 645 1242 Size: 645 597 AflIII A'CryG_T Cuts at: 0 338 1242 Size: 338 904 ApaI G_GGCC'C Cuts at: 0 142 681 1242 Size: 142 539 561 Fragments arranged by size: 561 539 142 AvaI C'yCGr_G Cuts at: 0 195 1242 Size: 195 1047 BamHI G'GATC_C Cuts at: 0 507 1242 Size: 507 735 BanI G'GyrC_C Cuts at: 0 42 75 103 1242 Size: 42 33 28 1139 Fragments arranged by size: 1139 42 33 28 BanII G_rGCy'C Cuts at: 0 142 569 681 1242 Size: 142 427 112 561 Fragments arranged by size: 561 427 142 112 BbeI G_GCGC'C Cuts at: 0 79 107 1242 Size: 79 28 1135 Fragments arranged by size: 1135 79 28 BfrBI ATG'CAT Cuts at: 0 599 1242 Size: 599 643 BglI GCCn_nnn'nGGC Cuts at: 0 73 137 163 182 191 1242 Size: 73 64 26 19 9 1051 Fragments arranged by size: 1051 73 64 26 19 9 Bme1580I G_kGCm'C Cuts at: 0 47 89 142 681 1242 Size: 47 42 53 539 561 Fragments arranged by size: 561 539 53 47 42 BmgBI CAC'GTC Cuts at: 0 578 795 1242 Size: 578 217 447 Fragments arranged by size: 578 447 217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 765 1242 Size: 765 477 Bpu10I CC'TnA_GC Cuts at: 0 331 1242 Size: 331 911 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 889 928 1242 Size: 889 39 314 Fragments arranged by size: 889 314 39 BsaI GGTCTCn'nnnn_ Cuts at: 0 985 1242 Size: 985 257 BsaAI yAC'GTr Cuts at: 0 20 284 341 1242 Size: 20 264 57 901 Fragments arranged by size: 901 264 57 20 BsaHI Gr'CG_yC Cuts at: 0 76 104 124 1242 Size: 76 28 20 1118 Fragments arranged by size: 1118 76 28 20 BsaWI w'CCGG_w Cuts at: 0 353 535 1242 Size: 353 182 707 Fragments arranged by size: 707 353 182 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1110 1113 1242 Size: 1110 3 129 Fragments arranged by size: 1110 129 3 BseYI C'CCAG_C Cuts at: 0 141 470 1242 Size: 141 329 772 Fragments arranged by size: 772 329 141 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 466 898 1242 Size: 466 432 344 Fragments arranged by size: 466 432 344 BsiEI CG_ry'CG Cuts at: 0 170 849 1242 Size: 170 679 393 Fragments arranged by size: 679 393 170 BsiHKAI G_wGCw'C Cuts at: 0 781 1242 Size: 781 461 BsmI GAATG_Cn' Cuts at: 0 771 1242 Size: 771 471 BsmBI CGTCTCn'nnnn_ Cuts at: 0 426 1122 1242 Size: 426 696 120 Fragments arranged by size: 696 426 120 Bsp1286I G_dGCh'C Cuts at: 0 47 89 142 569 681 781 1242 Size: 47 42 53 427 112 100 461 Fragments arranged by size: 461 427 112 100 53 47 42 BspEI T'CCGG_A Cuts at: 0 535 1242 Size: 535 707 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 713 1242 Size: 713 529 BsrBI CCG'CTC Cuts at: 0 260 804 1242 Size: 260 544 438 Fragments arranged by size: 544 438 260 BsrDI GCAATG_nn' Cuts at: 0 11 1242 Size: 11 1231 BsrFI r'CCGG_y Cuts at: 0 169 817 1242 Size: 169 648 425 Fragments arranged by size: 648 425 169 BssHII G'CGCG_C Cuts at: 0 558 1242 Size: 558 684 BstAPI GCAn_nnn'nTGC Cuts at: 0 389 596 1242 Size: 389 207 646 Fragments arranged by size: 646 389 207 BstYI r'GATC_y Cuts at: 0 507 878 1242 Size: 507 371 364 Fragments arranged by size: 507 371 364 BtgI C'CryG_G Cuts at: 0 480 1242 Size: 480 762 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 364 1242 Size: 364 878 BtsI GCAGTG_nn' Cuts at: 0 781 1242 Size: 781 461 DrdI GACnn_nn'nnGTC Cuts at: 0 853 1242 Size: 853 389 EaeI y'GGCC_r Cuts at: 0 129 167 174 273 644 809 1222 1242 Size: 129 38 7 99 371 165 413 20 Fragments arranged by size: 413 371 165 129 99 38 20 7 EagI C'GGCC_G Cuts at: 0 167 1242 Size: 167 1075 EarI CTCTTCn'nnn_ Cuts at: 0 664 1242 Size: 664 578 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 831 1242 Size: 831 411 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 645 765 1242 Size: 645 120 477 Fragments arranged by size: 645 477 120 EcoO109I rG'GnC_Cy Cuts at: 0 138 1242 Size: 138 1104 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 321 1242 Size: 321 921 HaeII r_GCGC'y Cuts at: 0 79 107 668 913 1242 Size: 79 28 561 245 329 Fragments arranged by size: 561 329 245 79 28 HincII GTy'rAC Cuts at: 0 921 1242 Size: 921 321 HindIII A'AGCT_T Cuts at: 0 1005 1084 1242 Size: 1005 79 158 Fragments arranged by size: 1005 158 79 KasI G'GCGC_C Cuts at: 0 75 103 1242 Size: 75 28 1139 Fragments arranged by size: 1139 75 28 MfeI C'AATT_G Cuts at: 0 1138 1242 Size: 1138 104 MscI TGG'CCA Cuts at: 0 176 1242 Size: 176 1066 MspA1I CmG'CkG Cuts at: 0 938 1242 Size: 938 304 NaeI GCC'GGC Cuts at: 0 819 1242 Size: 819 423 NarI GG'CG_CC Cuts at: 0 76 104 1242 Size: 76 28 1138 Fragments arranged by size: 1138 76 28 NgoMIV G'CCGG_C Cuts at: 0 817 1242 Size: 817 425 NsiI A_TGCA'T Cuts at: 0 601 1242 Size: 601 641 NspI r_CATG'y Cuts at: 0 603 829 1242 Size: 603 226 413 Fragments arranged by size: 603 413 226 PfoI T'CCnGG_A Cuts at: 0 1187 1242 Size: 1187 55 PmlI CAC'GTG Cuts at: 0 20 284 341 1242 Size: 20 264 57 901 Fragments arranged by size: 901 264 57 20 PspOMI G'GGCC_C Cuts at: 0 138 677 1242 Size: 138 539 565 Fragments arranged by size: 565 539 138 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 319 351 1242 Size: 319 32 891 Fragments arranged by size: 891 319 32 PstI C_TGCA'G Cuts at: 0 710 1242 Size: 710 532 PvuII CAG'CTG Cuts at: 0 938 1242 Size: 938 304 SapI GCTCTTCn'nnn_ Cuts at: 0 664 1242 Size: 664 578 ScaI AGT'ACT Cuts at: 0 397 1242 Size: 397 845 SexAI A'CCwGG_T Cuts at: 0 719 1242 Size: 719 523 SfcI C'TryA_G Cuts at: 0 706 1242 Size: 706 536 SfiI GGCCn_nnn'nGGCC Cuts at: 0 137 182 191 1242 Size: 137 45 9 1051 Fragments arranged by size: 1051 137 45 9 SfoI GGC'GCC Cuts at: 0 77 105 1242 Size: 77 28 1137 Fragments arranged by size: 1137 77 28 SmaI CCC'GGG Cuts at: 0 197 1242 Size: 197 1045 SmlI C'TyrA_G Cuts at: 0 904 943 1242 Size: 904 39 299 Fragments arranged by size: 904 299 39 SphI G_CATG'C Cuts at: 0 603 829 1242 Size: 603 226 413 Fragments arranged by size: 603 413 226 StuI AGG'CCT Cuts at: 0 67 1242 Size: 67 1175 StyI C'CwwG_G Cuts at: 0 132 1242 Size: 132 1110 TatI w'GTAC_w Cuts at: 0 395 932 1242 Size: 395 537 310 Fragments arranged by size: 537 395 310 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 184 615 1242 Size: 184 431 627 Fragments arranged by size: 627 431 184 XmaI C'CCGG_G Cuts at: 0 195 1242 Size: 195 1047 Enzymes that do cut: AcuI AflIII ApaI AvaI BamHI BanI BanII BbeI BfrBI BglI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstYI BtgI BtgZI BtsI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I FalI HaeII HincII HindIII KasI MfeI MscI MspA1I NaeI NarI NgoMIV NsiI NspI PfoI PmlI PspOMI PsrI PstI PvuII SapI ScaI SexAI SfcI SfiI SfoI SmaI SmlI SphI StuI StyI TatI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI BclI BglII BlpI BmrI BmtI BplI BsaBI BsaXI BsiWI BspHI BsrGI BssSI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HpaI KpnI MluI MmeI MslI NcoI NdeI NheI NotI NruI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PvuI RsrII SacI SacII SalI SanDI SbfI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TaqII TstI Tth111I XbaI XhoI XmnI ZraI