Restriction map for
pFN21AE0314
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I ApaI
ApaLI AscI Asp718I AspEI AspI
AviII BbeI BbrPI BbvCI BcgI
BclI BlpI Bpu1102I BsaAI BsaHI
BseAI BsiEI BsiWI BsmFI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII Cfr10I CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI FbaI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AflII 1036 CTTAAG C^TTAAG
AflIII 1456 ACACGT A^C[GA][CT]GT
AocI 1143 CCTTAGG CC^T[ACGT]AGG
Aor51HI 801 AGCGCT AGC^GCT
AseI 459 ATTAAT AT^TAAT
AsnI 459 ATTAAT AT^TAAT
Asp700I 837 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 556 CCTAGG C^CTAGG
BalI 2 TGGCCA TGG^CCA
BciVI 552 GTATCC GTATCC(6/5)
BfrI 1036 CTTAAG C^TTAAG
BglI 112 GCCCTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 421 AGATCT A^GATCT
BlnI 556 CCTAGG C^CTAGG
BsaBI 422 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 923 GGTCTC GGTCTC(1/5)
BsaWI 714 ACCGGA [AT]^CCGG[AT]
BsgI 1502 GTGCAG GTGCAG(16/14)
BsmBI 1975 GAGACG CGTCTC(1/5)
BspDI 1058 ATCGAT AT^CGAT
BspMI 1504 GCAGGT ACCTGC(4/8)
Bsu36I 1143 CCTTAGG CC^T[ACGT]AGG
ClaI 1058 ATCGAT AT^CGAT
DraIII 13 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1547 CCACGG C^C[GA][CT]GG
EaeI 2 TGGCCA [CT]^GGCC[GA]
Eco47III 801 AGCGCT AGC^GCT
Eco81I 1143 CCTTAGG CC^T[ACGT]AGG
EcoNI 481 CCTGATTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 781 GATATC GAT^ATC
HaeII 801 AGCGCT [GA]GCGC^[CT]
HindIII 2004 AAGCTT A^AGCTT
MaeII 1458 ACGT A^CGT
MamI 422 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 268 CACTCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 12 CCACCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 459 ATTAAT AT^TAAT
SapI 1126 GAAGAGC GCTCTTC(1/4)
SwaI 972 ATTTAAAT ATTT^AAAT
Van91I 12 CCACCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 837 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 74 CGCG CG^CG
94 CGCG
AlwNI 476 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
857 CAGTTCCTG
AspHI 589 GAGCTC G[AT]GC[AT]^C
1129 GAGCTC
AvaI 1010 CCCGGG C^[CT]CG[GA]G
1682 CCCGGG
AvaII 99 GGACC G^G[AT]CC
1296 GGACC
BamHI 822 GGATCC G^GATCC
1628 GGATCC
BanI 696 GGTGCC G^G[CT][GA]CC
1274 GGTGCC
BsiHKAI 589 GAGCTC G[AT]GC[AT]^C
1129 GAGCTC
BsoBI 1010 CCCGGG C^[CT]CG[GA]G
1682 CCCGGG
BstUI 74 CGCG CG^CG
94 CGCG
EcoT14I 556 CCTAGG C^C[AT][AT]GG
1477 CCAAGG
EcoT22I 1379 ATGCAT ATGCA^T
1391 ATGCAT
FauI 169 CCCGC CCCGC(4/6)
75 GCGGG
MvnI 74 CGCG CG^CG
94 CGCG
NsiI 1379 ATGCAT ATGCA^T
1391 ATGCAT
NspI 219 ACATGC [GA]CATG^[CT]
1389 ACATGC
PpuMI 98 AGGACCC [GA]G^G[AT]CC[CT]
1295 AGGACCT
PvuII 662 CAGCTG CAG^CTG
1021 CAGCTG
SacI 589 GAGCTC GAGCT^C
1129 GAGCTC
ScaI 1950 AGTACT AGT^ACT
1958 AGTACT
SmaI 1010 CCCGGG CCC^GGG
1682 CCCGGG
StyI 556 CCTAGG C^C[AT][AT]GG
1477 CCAAGG
TfiI 2080 GATTC G^A[AT]TC
2140 GAATC
XmaCI 1010 CCCGGG C^CCGGG
1682 CCCGGG
XmaI 1010 CCCGGG C^CCGGG
1682 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
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AciI AcsI AfaI AluI AlwI
ApoI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII