(Circular) (Six-base) MAPSORT of: pFN21AE0314.seq Check: 298 from: 1 to: 6853 pFN21AE0314 6853 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3500 3500 Size: 6853 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4663 278 Size: 53 83 186 936 3127 2468 Fragments arranged by size: 3127 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4196 1937 Size: 2259 4594 Acc65I G'GTAC_C Cuts at: 4174 4174 Size: 6853 AclI AA'CG_TT Cuts at: 1563 4980 5353 1563 Size: 3417 373 3063 Fragments arranged by size: 3417 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2547 2954 4017 4916 6300 6568 1632 Size: 915 407 1063 899 1384 268 1917 Fragments arranged by size: 1917 1384 1063 915 899 407 268 AfeI AGC'GCT Cuts at: 2808 2808 Size: 6853 AflII C'TTAA_G Cuts at: 820 1017 3041 820 Size: 197 2024 4632 Fragments arranged by size: 4632 2024 197 AflIII A'CryG_T Cuts at: 1203 3461 4150 5752 6729 1203 Size: 2258 689 1602 977 1327 Fragments arranged by size: 2258 1602 1327 977 689 AgeI A'CCGG_T Cuts at: 5684 5684 Size: 6853 AhdI GACnn_n'nnGTC Cuts at: 5582 5582 Size: 6853 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6853 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6853 AlwNI CAG_nnn'CTG Cuts at: 2486 2867 4596 6168 2486 Size: 381 1729 1572 3171 Fragments arranged by size: 3171 1729 1572 381 ApaLI G'TGCA_C Cuts at: 4910 6066 4910 Size: 1156 5697 ApoI r'AATT_y Cuts at: 1493 2846 2981 3417 3448 4162 4388 4540 6649 Size: 1353 135 436 31 714 226 152 2109 Cuts at: 6649 1493 Size: 1697 Fragments arranged by size: 2109 1697 1353 714 436 226 152 135 31 AseI AT'TA_AT Cuts at: 160 1032 2465 5407 160 Size: 872 1433 2942 1606 Fragments arranged by size: 2942 1606 1433 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6853 AvaI C'yCGr_G Cuts at: 1870 1943 3015 3687 4178 1870 Size: 73 1072 672 491 4545 Fragments arranged by size: 4545 1072 672 491 73 AvrII C'CTAG_G Cuts at: 2561 2561 Size: 6853 BamHI G'GATC_C Cuts at: 2827 3633 4183 4550 2827 Size: 806 550 367 5130 Fragments arranged by size: 5130 806 550 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2701 3279 4174 5629 618 Size: 325 217 627 914 578 895 1455 1842 Fragments arranged by size: 1842 1455 914 895 627 578 325 217 BanII G_rGCy'C Cuts at: 1391 2598 3138 3169 4172 1391 Size: 1207 540 31 1003 4072 Fragments arranged by size: 4072 1207 1003 540 31 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2409 2493 4005 928 Size: 971 510 84 1512 3776 Fragments arranged by size: 3776 1512 971 510 84 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5044 5078 1903 Size: 34 3107 34 3678 Fragments arranged by size: 3678 3107 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2568 4745 5955 2568 Size: 2177 1210 3466 Fragments arranged by size: 3466 2177 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6853 BfrBI ATG'CAT Cuts at: 3386 3398 3386 Size: 12 6841 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2123 5464 136 Size: 107 122 71 631 605 451 3341 1525 Fragments arranged by size: 3341 1525 631 605 451 122 107 71 BglII A'GATC_T Cuts at: 2426 6848 2426 Size: 4422 2431 BlpI GC'TnA_GC Cuts at: 4270 4270 Size: 6853 Bme1580I G_kGCm'C Cuts at: 1790 2706 3284 3552 4914 6070 1790 Size: 916 578 268 1362 1156 2573 Fragments arranged by size: 2573 1362 1156 916 578 268 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6853 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2083 4584 5542 Size: 416 53 83 399 24 666 2501 958 Cuts at: 5542 442 Size: 1753 Fragments arranged by size: 2501 1753 958 666 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6853 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2946 2946 Size: 6853 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2026 2033 2094 5513 1239 Size: 787 7 61 3419 2579 Fragments arranged by size: 3419 2579 787 61 7 Bpu10I CC'TnA_GC Cuts at: 2589 3693 2589 Size: 1104 5749 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3617 3980 4978 5843 6141 3617 Size: 363 998 865 298 4329 Fragments arranged by size: 4329 998 865 363 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2934 5516 882 Size: 617 1435 2582 2219 Fragments arranged by size: 2582 2219 1435 617 BsaAI yAC'GTr Cuts at: 493 1204 4644 493 Size: 711 3440 2702 Fragments arranged by size: 3440 2702 711 BsaBI GATnn'nnATC Cuts at: 2431 6847 2431 Size: 4416 2437 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4660 5042 5677 275 Size: 53 83 186 936 3127 382 635 1451 Fragments arranged by size: 3127 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2719 5286 5684 5958 6105 6516 2719 Size: 2567 398 274 147 411 3056 Fragments arranged by size: 3056 2567 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2067 2097 1178 Size: 30 165 30 664 30 5934 Fragments arranged by size: 5934 664 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2168 2171 2297 2512 2546 2777 3183 1186 Size: 982 3 126 215 34 231 406 4856 Fragments arranged by size: 4856 982 406 231 215 126 34 3 BseYI C'CCAG_C Cuts at: 2177 6056 6552 2177 Size: 3879 496 2478 Fragments arranged by size: 3879 2478 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3528 1163 Size: 573 1792 4488 Fragments arranged by size: 4488 1792 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4303 5064 6092 1540 Size: 275 186 2302 761 1028 2301 Fragments arranged by size: 2302 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2598 3138 4914 4999 6070 2598 Size: 540 1776 85 1071 3381 Fragments arranged by size: 3381 1776 1071 540 85 BsmI GAATG_Cn' Cuts at: 4364 4457 4364 Size: 93 6760 BsmBI CGTCTCn'nnnn_ Cuts at: 3974 3974 Size: 6853 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2598 2706 3138 3169 3284 3552 4172 Size: 399 808 108 432 31 115 268 620 Cuts at: 4172 4914 4999 6070 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 808 742 620 432 399 268 115 108 85 31 BspEI T'CCGG_A Cuts at: 6516 6516 Size: 6853 BspHI T'CATG_A Cuts at: 4742 6472 4742 Size: 1730 5123 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3500 4208 844 Size: 2656 708 3489 Fragments arranged by size: 3489 2656 708 BsrBI CCG'CTC Cuts at: 1394 1823 4740 1394 Size: 429 2917 3507 Fragments arranged by size: 3507 2917 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5348 5522 65 Size: 1183 4100 174 1396 Fragments arranged by size: 4100 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5497 5684 1700 Size: 110 3687 187 2869 Fragments arranged by size: 3687 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6853 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6853 BssSI C'ACGA_G Cuts at: 4913 5925 4913 Size: 1012 5841 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6853 BstBI TT'CG_AA Cuts at: 5657 5657 Size: 6853 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6853 BstYI r'GATC_y Cuts at: 1972 2426 2827 3633 4183 4550 4935 4952 6404 Size: 454 401 806 550 367 385 17 1452 Cuts at: 6404 6490 6502 6848 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 806 550 454 401 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3149 3149 Size: 6853 BtgI C'CryG_G Cuts at: 513 1065 2004 3552 513 Size: 552 939 1548 3814 Fragments arranged by size: 3814 1548 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6114 Fragments arranged by size: 6114 683 56 BtsI GCAGTG_nn' Cuts at: 3830 4369 5163 5183 3830 Size: 539 794 20 5500 Fragments arranged by size: 5500 794 539 20 ClaI AT'CG_AT Cuts at: 1554 3064 1554 Size: 1510 5343 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6818 DraI TTT'AAA Cuts at: 2373 2980 3070 3453 4157 2373 Size: 607 90 383 704 5069 Fragments arranged by size: 5069 704 607 383 90 DraIII CAC_nnn'GTG Cuts at: 2023 2023 Size: 6853 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5860 809 Size: 507 4544 1802 Fragments arranged by size: 4544 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 4300 5189 Size: 54 1425 50 275 18 177 2293 889 Cuts at: 5189 6818 8 Size: 1629 43 Fragments arranged by size: 2293 1629 1425 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4300 1537 Size: 275 2488 4090 Fragments arranged by size: 4090 2488 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2145 2298 2425 2523 2817 3126 3615 4122 Size: 792 153 127 98 294 309 489 507 Cuts at: 4122 4783 1353 Size: 661 3423 Fragments arranged by size: 3423 792 661 507 489 309 294 153 127 98 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5440 5814 5960 387 Size: 1064 138 3851 374 146 1280 Fragments arranged by size: 3851 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2596 3136 2596 Size: 540 6313 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2026 2033 2094 2547 2954 4017 4916 Size: 393 394 7 61 453 407 1063 899 Cuts at: 4916 5513 6300 6568 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1063 899 787 597 453 407 394 393 268 61 7 EcoNI CCTnn'n_nnAGG Cuts at: 2490 2490 Size: 6853 EcoO109I rG'GnC_Cy Cuts at: 2104 2116 3301 2104 Size: 12 1185 5656 Fragments arranged by size: 5656 1185 12 EcoRI G'AATT_C Cuts at: 4162 4540 4162 Size: 378 6475 EcoRV GAT'ATC Cuts at: 2788 2788 Size: 6853 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2426 2972 2426 Size: 546 6307 FspI TGC'GCA Cuts at: 5359 5359 Size: 6853 HaeII r_GCGC'y Cuts at: 1678 2810 6000 1678 Size: 1132 3190 2531 Fragments arranged by size: 3190 2531 1132 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2067 2099 5494 5526 5568 Size: 32 135 32 495 32 3395 32 42 Cuts at: 5568 5600 1373 Size: 32 2626 Fragments arranged by size: 3395 2626 495 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4197 4443 669 Size: 988 281 2259 246 3079 Fragments arranged by size: 3079 2259 988 281 246 HindIII A'AGCT_T Cuts at: 748 4009 748 Size: 3261 3592 HpaI GTT'AAC Cuts at: 4443 4443 Size: 6853 KpnI G_GTAC'C Cuts at: 4178 4178 Size: 6853 MfeI C'AATT_G Cuts at: 4452 4452 Size: 6853 MluI A'CGCG_T Cuts at: 6729 6729 Size: 6853 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3594 5967 6151 1305 Size: 2289 2373 184 2007 Fragments arranged by size: 2373 2289 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 4854 Fragments arranged by size: 4854 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2277 4811 5170 5329 518 Size: 648 1111 2534 359 159 2042 Fragments arranged by size: 2534 2042 1111 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2129 2177 2669 3028 4269 4946 6094 6339 Size: 523 48 492 359 1241 677 1148 245 Cuts at: 6339 1606 Size: 2120 Fragments arranged by size: 2120 1241 1148 677 523 492 359 245 48 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5362 Fragments arranged by size: 5362 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6853 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6853 NotI GC'GGCC_GC Cuts at: 4300 4300 Size: 6853 NsiI A_TGCA'T Cuts at: 3388 3400 3388 Size: 12 6841 NspI r_CATG'y Cuts at: 1132 1229 2228 3398 4211 5756 1132 Size: 97 999 1170 813 1545 2229 Fragments arranged by size: 2229 1545 1170 999 813 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6853 PciI A'CATG_T Cuts at: 5752 5752 Size: 6853 PflMI CCAn_nnn'nTGG Cuts at: 1108 2023 1108 Size: 915 5938 PmeI GTTT'AAAC Cuts at: 4157 4157 Size: 6853 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4922 4954 6435 6467 4922 Size: 32 1481 32 5308 Fragments arranged by size: 5308 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2104 3301 2104 Size: 1197 5656 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6853 PsiI TTA'TAA Cuts at: 4423 4423 Size: 6853 PstI C_TGCA'G Cuts at: 830 4205 830 Size: 3375 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6853 PvuII CAG'CTG Cuts at: 2669 3028 2669 Size: 359 6494 SacI G_AGCT'C Cuts at: 2598 3138 2598 Size: 540 6313 SalI G'TCGA_C Cuts at: 1936 4195 1936 Size: 2259 4594 SapI GCTCTTCn'nnn_ Cuts at: 3126 3126 Size: 6853 SbfI CC_TGCA'GG Cuts at: 4205 4205 Size: 6853 ScaI AGT'ACT Cuts at: 3957 3965 5101 6813 3957 Size: 8 1136 1712 3997 Fragments arranged by size: 3997 1712 1136 8 SfcI C'TryA_G Cuts at: 826 1045 4201 5336 6017 6208 826 Size: 219 3156 1135 681 191 1471 Fragments arranged by size: 3156 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6853 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6853 SmaI CCC'GGG Cuts at: 1872 3017 3689 4180 1872 Size: 1145 672 491 4545 Fragments arranged by size: 4545 1145 672 491 SmlI C'TyrA_G Cuts at: 820 1017 1943 3041 3596 3959 4957 5858 6120 Size: 197 926 1098 555 363 998 901 262 Cuts at: 6120 820 Size: 1553 Fragments arranged by size: 1553 1098 998 926 901 555 363 262 197 SnaBI TAC'GTA Cuts at: 493 4644 493 Size: 4151 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6853 SphI G_CATG'C Cuts at: 1132 4211 1132 Size: 3079 3774 SspI AAT'ATT Cuts at: 5 52 4777 5 Size: 47 4725 2081 Fragments arranged by size: 4725 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6853 StyI C'CwwG_G Cuts at: 513 1065 2004 2561 3482 4292 513 Size: 552 939 557 921 810 3074 Fragments arranged by size: 3074 939 921 810 557 552 SwaI ATTT'AAAT Cuts at: 2980 2980 Size: 6853 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2689 4880 4897 5050 5235 5680 325 Size: 1255 1109 2191 17 153 185 445 1498 Fragments arranged by size: 2191 1498 1255 1109 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3955 3963 5099 Size: 274 80 33 51 1443 1978 8 1136 Cuts at: 5099 6811 96 Size: 1712 138 Fragments arranged by size: 1978 1712 1443 1136 274 138 80 51 33 8 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6813 6845 6813 Size: 32 6821 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6853 XbaI T'CTAG_A Cuts at: 4189 4189 Size: 6853 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6853 XmaI C'CCGG_G Cuts at: 1870 3015 3687 4178 1870 Size: 1145 672 491 4545 Fragments arranged by size: 4545 1145 672 491 XmnI GAAnn'nnTTC Cuts at: 2846 4982 2846 Size: 2136 4717 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4661 276 Size: 53 83 186 936 3127 2468 Fragments arranged by size: 3127 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BaeI BbeI BbvCI BsiWI BstXI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI XcmI