Restriction map for
pFN21AE0314
(Linear) (Six-base) MAPSORT of: KIEE0314.seq Check: 4981 from: 1 to: 2148
KIEE0314.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1495 2148
Size: 1495 653
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 949 2012 2148
Size: 542 407 1063 136
Fragments arranged by size:
1063 542 407 136
AfeI AGC'GCT
Cuts at: 0 803 2148
Size: 803 1345
AflII C'TTAA_G
Cuts at: 0 1036 2148
Size: 1036 1112
AflIII A'CryG_T
Cuts at: 0 1456 2148
Size: 1456 692
AlwNI CAG_nnn'CTG
Cuts at: 0 481 862 2148
Size: 481 381 1286
Fragments arranged by size:
1286 481 381
ApoI r'AATT_y
Cuts at: 0 841 976 1412 1443 2148
Size: 841 135 436 31 705
Fragments arranged by size:
841 705 436 135 31
AseI AT'TA_AT
Cuts at: 0 460 2148
Size: 460 1688
AvaI C'yCGr_G
Cuts at: 0 1010 1682 2148
Size: 1010 672 466
Fragments arranged by size:
1010 672 466
AvrII C'CTAG_G
Cuts at: 0 556 2148
Size: 556 1592
BamHI G'GATC_C
Cuts at: 0 822 1628 2148
Size: 822 806 520
Fragments arranged by size:
822 806 520
BanI G'GyrC_C
Cuts at: 0 696 1274 2148
Size: 696 578 874
Fragments arranged by size:
874 696 578
BanII G_rGCy'C
Cuts at: 0 593 1133 1164 2148
Size: 593 540 31 984
Fragments arranged by size:
984 593 540 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 404 488 2000 2148
Size: 404 84 1512 148
Fragments arranged by size:
1512 404 148 84
BciVI GTATCCnnnnn_n'
Cuts at: 0 563 2148
Size: 563 1585
BfrBI ATG'CAT
Cuts at: 0 1381 1393 2148
Size: 1381 12 755
Fragments arranged by size:
1381 755 12
BglI GCCn_nnn'nGGC
Cuts at: 0 118 2148
Size: 118 2030
BglII A'GATC_T
Cuts at: 0 421 2148
Size: 421 1727
Bme1580I G_kGCm'C
Cuts at: 0 701 1279 1547 2148
Size: 701 578 268 601
Fragments arranged by size:
701 601 578 268
BmrI ACTGGGnnnn_n'
Cuts at: 0 78 2148
Size: 78 2070
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 941 2148
Size: 941 1207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 28 89 2148
Size: 21 7 61 2059
Fragments arranged by size:
2059 61 21 7
Bpu10I CC'TnA_GC
Cuts at: 0 584 1688 2148
Size: 584 1104 460
Fragments arranged by size:
1104 584 460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1612 1975 2148
Size: 1612 363 173
Fragments arranged by size:
1612 363 173
BsaI GGTCTCn'nnnn_
Cuts at: 0 929 2148
Size: 929 1219
BsaBI GATnn'nnATC
Cuts at: 0 426 2148
Size: 426 1722
BsaWI w'CCGG_w
Cuts at: 0 714 2148
Size: 714 1434
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 62 92 2148
Size: 62 30 2056
Fragments arranged by size:
2056 62 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 166 292 507 541 772 1178 2148
Size: 163 3 126 215 34 231 406 970
Fragments arranged by size:
970 406 231 215 163 126 34 3
BseYI C'CCAG_C
Cuts at: 0 172 2148
Size: 172 1976
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1523 2148
Size: 1523 625
BsiHKAI G_wGCw'C
Cuts at: 0 593 1133 2148
Size: 593 540 1015
Fragments arranged by size:
1015 593 540
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1969 2148
Size: 1969 179
Bsp1286I G_dGCh'C
Cuts at: 0 593 701 1133 1164 1279 1547 2148
Size: 593 108 432 31 115 268 601
Fragments arranged by size:
601 593 432 268 115 108 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1495 2148
Size: 1495 653
BstYI r'GATC_y
Cuts at: 0 421 822 1628 2148
Size: 421 401 806 520
Fragments arranged by size:
806 520 421 401
Bsu36I CC'TnA_GG
Cuts at: 0 1144 2148
Size: 1144 1004
BtgI C'CryG_G
Cuts at: 0 1547 2148
Size: 1547 601
BtsI GCAGTG_nn'
Cuts at: 0 1825 2148
Size: 1825 323
ClaI AT'CG_AT
Cuts at: 0 1059 2148
Size: 1059 1089
DraI TTT'AAA
Cuts at: 0 368 975 1065 1448 2148
Size: 368 607 90 383 700
Fragments arranged by size:
700 607 383 368 90
DraIII CAC_nnn'GTG
Cuts at: 0 18 2148
Size: 18 2130
EaeI y'GGCC_r
Cuts at: 0 2 2148
Size: 2 2146
EarI CTCTTCn'nnn_
Cuts at: 0 140 293 420 518 812 1121 1610 2117
Size: 140 153 127 98 294 309 489 507
Cuts at: 2117 2148
Size: 31
Fragments arranged by size:
507 489 309 294 153 140 127 98
31
EcoICRI GAG'CTC
Cuts at: 0 591 1131 2148
Size: 591 540 1017
Fragments arranged by size:
1017 591 540
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 28 89 542 949 2012 2148
Size: 21 7 61 453 407 1063 136
Fragments arranged by size:
1063 453 407 136 61 21 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 485 2148
Size: 485 1663
EcoO109I rG'GnC_Cy
Cuts at: 0 99 111 1296 2148
Size: 99 12 1185 852
Fragments arranged by size:
1185 852 99 12
EcoRV GAT'ATC
Cuts at: 0 783 2148
Size: 783 1365
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 421 967 2148
Size: 421 546 1181
Fragments arranged by size:
1181 546 421
HaeII r_GCGC'y
Cuts at: 0 805 2148
Size: 805 1343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 62 94 2148
Size: 62 32 2054
Fragments arranged by size:
2054 62 32
HindIII A'AGCT_T
Cuts at: 0 2004 2148
Size: 2004 144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1589 2148
Size: 1589 559
MscI TGG'CCA
Cuts at: 0 4 2148
Size: 4 2144
MslI CAynn'nnrTG
Cuts at: 0 272 2148
Size: 272 1876
MspA1I CmG'CkG
Cuts at: 0 124 172 664 1023 2148
Size: 124 48 492 359 1125
Fragments arranged by size:
1125 492 359 124 48
NsiI A_TGCA'T
Cuts at: 0 1383 1395 2148
Size: 1383 12 753
Fragments arranged by size:
1383 753 12
NspI r_CATG'y
Cuts at: 0 223 1393 2148
Size: 223 1170 755
Fragments arranged by size:
1170 755 223
PflMI CCAn_nnn'nTGG
Cuts at: 0 18 2148
Size: 18 2130
PpuMI rG'GwC_Cy
Cuts at: 0 99 1296 2148
Size: 99 1197 852
Fragments arranged by size:
1197 852 99
PvuII CAG'CTG
Cuts at: 0 664 1023 2148
Size: 664 359 1125
Fragments arranged by size:
1125 664 359
SacI G_AGCT'C
Cuts at: 0 593 1133 2148
Size: 593 540 1015
Fragments arranged by size:
1015 593 540
SapI GCTCTTCn'nnn_
Cuts at: 0 1121 2148
Size: 1121 1027
ScaI AGT'ACT
Cuts at: 0 1952 1960 2148
Size: 1952 8 188
Fragments arranged by size:
1952 188 8
SmaI CCC'GGG
Cuts at: 0 1012 1684 2148
Size: 1012 672 464
Fragments arranged by size:
1012 672 464
SmlI C'TyrA_G
Cuts at: 0 1036 1591 1954 2148
Size: 1036 555 363 194
Fragments arranged by size:
1036 555 363 194
StyI C'CwwG_G
Cuts at: 0 556 1477 2148
Size: 556 921 671
Fragments arranged by size:
921 671 556
SwaI ATTT'AAAT
Cuts at: 0 975 2148
Size: 975 1173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 684 2148
Size: 684 1464
TatI w'GTAC_w
Cuts at: 0 1950 1958 2148
Size: 1950 8 190
Fragments arranged by size:
1950 190 8
XmaI C'CCGG_G
Cuts at: 0 1010 1682 2148
Size: 1010 672 466
Fragments arranged by size:
1010 672 466
XmnI GAAnn'nnTTC
Cuts at: 0 841 2148
Size: 841 1307
Enzymes that do cut:
AarI AcuI AfeI AflII AflIII AlwNI ApoI AseI
AvaI AvrII BamHI BanI BanII BbsI BciVI BfrBI
BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspMI BstYI Bsu36I BtgI BtsI ClaI
DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI HaeII Hin4I HindIII MmeI MscI MslI
MspA1I NsiI NspI PflMI PpuMI PvuII SacI SapI
ScaI SmaI SmlI StyI SwaI TaqII TatI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AgeI AhdI AleI AlfI
AloI ApaI ApaLI AscI AsiSI BaeI BbeI BbvCI
BcgI BclI BlpI BmgBI BmtI BsaAI BsaHI BsiEI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI
CspCI DrdI EagI EciI EcoRI FseI FspI FspAI
HincII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI TstI Tth111I XbaI
XcmI XhoI ZraI