################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AccI AclI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaI BsaJI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI Bsp1286I Bsp1407I BspDI BsrBI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HincII HindII HindIII HpaI HphI KasI Ksp632I KspI MaeI MamI MfeI MflI MluI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 72 enzymes) # ################################### AatII 578 GACGTC GACGT^C Acc65I 480 GGTACC G^GTACC AccII 51 CGCG CG^CG AccIII 445 TCCGGA T^CCGGA AciI 47 GCGG CCGC(-3/-1) AcsI 200 GAATTC [GA]^AATT[CT] AcyI 578 GACGTC G[GA]^CG[CT]C AflIII 215 ACATGT A^C[GA][CT]GT AocI 502 CCTGAGG CC^T[ACGT]AGG ApoI 200 GAATTC [GA]^AATT[CT] Asp700I 196 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 480 GGTACC G^GTACC AvaI 17 CTCGGG C^[CT]CG[GA]G AvaII 253 GGACC G^G[AT]CC BalI 368 TGGCCA TGG^CCA BanI 480 GGTACC G^G[CT][GA]CC BbsI 211 GAAGAC GAAGAC(2/6) BbvI 358 GCTGC GCAGC(8/12) BcnI 38 CCCGG CC^[GC]GG BpmI 131 CTGGAG CTGGAG(16/14) BpuAI 211 GAAGAC GAAGAC(2/6) BsaHI 578 GACGTC G[GA]^CG[CT]C BsaWI 445 TCCGGA [AT]^CCGG[AT] BseAI 445 TCCGGA T^CCGGA BsmAI 26 GAGAC GTCTC(1/5) BsmFI 127 GGGAC GGGAC(10/14) BsmI 306 GAATGC GAATGC(1/-1) BsoBI 17 CTCGGG C^[CT]CG[GA]G BspEI 445 TCCGGA T^CCGGA BspHI 302 TCATGA T^CATGA BspLU11I 215 ACATGT A^CATGT BspMI 328 GCAGGT ACCTGC(4/8) BssHII 50 GCGCGC G^CGCGC BstF5I 67 CATCC GGATG(2/0) BstNI 385 CCAGG CC^[AT]GG BstUI 51 CGCG CG^CG Bsu36I 502 CCTGAGG CC^T[ACGT]AGG Cac8I 50 GCGCGC GC[ACGT]^[ACGT]GC Cfr13I 253 GGACC G^G[ACGT]CC DdeI 503 CTGAG C^T[ACGT]AG DraI 397 TTTAAA TTT^AAA EaeI 368 TGGCCA [CT]^GGCC[GA] Eco81I 502 CCTGAGG CC^T[ACGT]AGG EcoRI 200 GAATTC G^AATTC EcoRII 385 CCAGG ^CC[AT]GG FokI 67 CATCC GGATG(9/13) Hin1I 578 GACGTC G[GA]^CG[CT]C KpnI 480 GGTACC GGTAC^C MaeII 579 ACGT A^CGT MaeIII 121 GTCAC ^GT[ACGT]AC MluNI 368 TGGCCA TGG^CCA MroI 445 TCCGGA T^CCGGA MscI 368 TGGCCA TGG^CCA MseI 398 TTAA T^TAA MslI 154 CATGGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 385 CCAGG CC^[AT]GG MvnI 51 CGCG CG^CG MwoI 361 GCTGTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 38 CCCGG CC^[GC]GG NlaIV 480 GGTACC GG[ACGT]^[ACGT]CC NspI 215 ACATGT [GA]CATG^[CT] PleI 556 GACTC GAGTC(4/5) RcaI 302 TCATGA T^CATGA Sau96I 253 GGACC G^G[ACGT]CC ScaI 567 AGTACT AGT^ACT TaqI 265 TCGA T^CGA TfiI 339 GATTC G^A[AT]TC Tru9I 398 TTAA T^TAA TseI 358 GCTGC G^C[AT]GC Tsp45I 121 GTCAC ^GT[GC]AC TthHB8I 265 TCGA T^CGA XmnI 196 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwI 81 GATCC GGATC(4/5) 688 GATCC BsgI 332 GTGCAG GTGCAG(16/14) 563 GTGCAG BsrDI 171 CATTGC GCAATG(2/0) 538 GCAATG BssKI 38 CCCGG ^CC[ACGT]GG 385 CCAGG CfoI 50 GCGC GCG^C 52 GCGC DpnII 81 GATC ^GATC 688 GATC Fnu4HI 47 GCGGC GC^[ACGT]GC 358 GCTGC HaeIII 369 GGCC GG^CC 618 GGCC HapII 39 CCGG C^CGG 446 CCGG HhaI 50 GCGC GCG^C 52 GCGC HinP1I 50 GCGC G^CGC 52 GCGC HinfI 339 GATTC G^A[ACGT]TC 556 GACTC HpaII 39 CCGG C^CGG 446 CCGG ItaI 47 GCGGC GC^[ACGT]GC 358 GCTGC MboI 81 GATC ^GATC 688 GATC MspI 39 CCGG C^CGG 446 CCGG NdeII 81 GATC ^GATC 688 GATC Sau3AI 81 GATC ^GATC 688 GATC ScrFI 38 CCCGG CC^[ACGT]GG 385 CCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AfaI AluI BseRI BsrI MboII MnlI NlaIII RsaI SfaNI Tsp509I TspRI