(Circular) (Six-base) MAPSORT of: pFN21AE1736.seq Check: 4225 from: 1 to: 5431 pFN21AE1736 5431 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2324 2324 Size: 5431 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2587 3241 278 Size: 53 83 186 936 1051 654 2468 Fragments arranged by size: 2468 1051 936 654 186 83 53 AccI GT'mk_AC Cuts at: 1937 2774 1937 Size: 837 4594 Acc65I G'GTAC_C Cuts at: 2485 2752 2485 Size: 267 5164 AclI AA'CG_TT Cuts at: 1563 3558 3931 1563 Size: 1995 373 3063 Fragments arranged by size: 3063 1995 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2169 2235 3494 4878 5146 1632 Size: 537 66 1259 1384 268 1917 Fragments arranged by size: 1917 1384 1259 537 268 66 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5234 AflIII A'CryG_T Cuts at: 1203 2220 4330 5307 1203 Size: 1017 2110 977 1327 Fragments arranged by size: 2110 1327 1017 977 AgeI A'CCGG_T Cuts at: 4262 4262 Size: 5431 AhdI GACnn_n'nnGTC Cuts at: 4160 4160 Size: 5431 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5431 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5431 AlwNI CAG_nnn'CTG Cuts at: 3174 4746 3174 Size: 1572 3859 ApaLI G'TGCA_C Cuts at: 3488 4644 3488 Size: 1156 4275 ApoI r'AATT_y Cuts at: 1493 2205 2740 2966 3118 5227 1493 Size: 712 535 226 152 2109 1697 Fragments arranged by size: 2109 1697 712 535 226 152 AseI AT'TA_AT Cuts at: 160 1032 3985 160 Size: 872 2953 1606 Fragments arranged by size: 2953 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 AvaI C'yCGr_G Cuts at: 1870 1943 2022 2756 1870 Size: 73 79 734 4545 Fragments arranged by size: 4545 734 79 73 BamHI G'GATC_C Cuts at: 2761 3128 2761 Size: 367 5064 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2485 2752 4207 618 Size: 325 217 627 698 267 1455 1842 Fragments arranged by size: 1842 1455 698 627 325 267 217 BanII G_rGCy'C Cuts at: 1391 2750 1391 Size: 1359 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2223 928 Size: 971 324 4136 Fragments arranged by size: 4136 971 324 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3622 3656 1903 Size: 34 1685 34 3678 Fragments arranged by size: 3678 1685 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3323 4533 3323 Size: 1210 4221 BclI T'GATC_A Cuts at: 1548 1548 Size: 5431 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4042 136 Size: 107 122 71 631 605 2370 1525 Fragments arranged by size: 2370 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5426 5426 Size: 5431 BlpI GC'TnA_GC Cuts at: 2848 2848 Size: 5431 Bme1580I G_kGCm'C Cuts at: 1790 3492 4648 1790 Size: 1702 1156 2573 Fragments arranged by size: 2573 1702 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5431 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2139 2702 3162 Size: 416 53 83 399 24 722 563 460 Cuts at: 3162 4120 442 Size: 958 1753 Fragments arranged by size: 1753 958 722 563 460 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5431 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2157 4091 1239 Size: 918 1934 2579 Fragments arranged by size: 2579 1934 918 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3556 4421 4719 3556 Size: 865 298 4268 Fragments arranged by size: 4268 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4094 882 Size: 617 2595 2219 Fragments arranged by size: 2595 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3222 493 Size: 711 2018 2702 Fragments arranged by size: 2702 2018 711 BsaBI GATnn'nnATC Cuts at: 5425 5425 Size: 5431 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2584 3238 3620 4255 Size: 53 83 186 936 1051 654 382 635 Cuts at: 4255 275 Size: 1451 Fragments arranged by size: 1451 1051 936 654 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2450 3864 4262 4536 4683 5094 2450 Size: 1414 398 274 147 411 2787 Fragments arranged by size: 2787 1414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2546 2576 2583 2613 1178 Size: 30 165 30 1143 30 7 30 3996 Fragments arranged by size: 3996 1143 165 30 30 30 30 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2154 2289 2343 2525 1186 Size: 968 135 54 182 4092 Fragments arranged by size: 4092 968 182 135 54 BseYI C'CCAG_C Cuts at: 2121 4634 5130 2121 Size: 2513 496 2422 Fragments arranged by size: 2513 2422 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2358 2589 1163 Size: 573 622 231 4005 Fragments arranged by size: 4005 622 573 231 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2881 3642 4670 1540 Size: 275 186 880 761 1028 2301 Fragments arranged by size: 2301 1028 880 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3492 3577 4648 3492 Size: 85 1071 4275 Fragments arranged by size: 4275 1071 85 BsmI GAATG_Cn' Cuts at: 2317 2942 3035 2317 Size: 625 93 4713 Fragments arranged by size: 4713 625 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2750 3492 3577 4648 1391 Size: 399 960 742 85 1071 2174 Fragments arranged by size: 2174 1071 960 742 399 85 BspEI T'CCGG_A Cuts at: 2450 5094 2450 Size: 2644 2787 BspHI T'CATG_A Cuts at: 2307 3320 5050 2307 Size: 1013 1730 2688 Fragments arranged by size: 2688 1730 1013 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2324 2786 844 Size: 1480 462 3489 Fragments arranged by size: 3489 1480 462 BsrBI CCG'CTC Cuts at: 1394 1823 3318 1394 Size: 429 1495 3507 Fragments arranged by size: 3507 1495 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2175 2550 3926 4100 65 Size: 1183 927 375 1376 174 1396 Fragments arranged by size: 1396 1376 1183 927 375 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4075 4262 1700 Size: 110 2265 187 2869 Fragments arranged by size: 2869 2265 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5431 BssHII G'CGCG_C Cuts at: 1925 2055 1925 Size: 130 5301 BssSI C'ACGA_G Cuts at: 3491 4503 3491 Size: 1012 4419 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5431 BstBI TT'CG_AA Cuts at: 4235 4235 Size: 5431 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5431 BstYI r'GATC_y Cuts at: 1972 2761 3128 3513 3530 4982 5068 5080 5426 Size: 789 367 385 17 1452 86 12 346 Cuts at: 5426 1972 Size: 1977 Fragments arranged by size: 1977 1452 789 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2508 2508 Size: 5431 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4879 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2178 487 Size: 683 56 952 3740 Fragments arranged by size: 3740 952 683 56 BtsI GCAGTG_nn' Cuts at: 2346 2947 3741 3761 2346 Size: 601 794 20 4016 Fragments arranged by size: 4016 794 601 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5431 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5396 DraI TTT'AAA Cuts at: 2404 2735 2404 Size: 331 5100 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4438 809 Size: 507 3122 1802 Fragments arranged by size: 3122 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2373 2878 3767 Size: 54 1425 50 275 18 543 505 889 Cuts at: 3767 5396 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 543 505 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2878 1537 Size: 275 1066 4090 Fragments arranged by size: 4090 1066 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3361 1353 Size: 2008 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4018 4392 4538 387 Size: 1064 138 2429 374 146 1280 Fragments arranged by size: 2429 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2157 2169 2235 3494 4091 4878 5146 Size: 393 525 12 66 1259 597 787 268 Cuts at: 5146 1239 Size: 1524 Fragments arranged by size: 1524 1259 787 597 525 393 268 66 12 EcoRI G'AATT_C Cuts at: 2205 2740 3118 2205 Size: 535 378 4518 Fragments arranged by size: 4518 535 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2277 2705 2277 Size: 428 5003 FspI TGC'GCA Cuts at: 3937 3937 Size: 5431 HaeII r_GCGC'y Cuts at: 1678 4578 1678 Size: 2900 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2070 2102 4072 4104 4146 Size: 32 135 32 498 32 1970 32 42 Cuts at: 4146 4178 1373 Size: 32 2626 Fragments arranged by size: 2626 1970 498 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2775 3021 669 Size: 988 281 837 246 3079 Fragments arranged by size: 3079 988 837 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5431 HpaI GTT'AAC Cuts at: 3021 3021 Size: 5431 KpnI G_GTAC'C Cuts at: 2489 2756 2489 Size: 267 5164 MfeI C'AATT_G Cuts at: 3030 3030 Size: 5431 MluI A'CGCG_T Cuts at: 5307 5307 Size: 5431 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2692 4545 4729 1305 Size: 1387 1853 184 2007 Fragments arranged by size: 2007 1853 1387 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2375 10 Size: 54 1425 343 543 3066 Fragments arranged by size: 3066 1425 543 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2163 3389 3748 3907 518 Size: 648 997 1226 359 159 2042 Fragments arranged by size: 2042 1226 997 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2847 3524 4672 4917 1606 Size: 1241 677 1148 245 2120 Fragments arranged by size: 2120 1241 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4879 NdeI CA'TA_TG Cuts at: 387 387 Size: 5431 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5431 NotI GC'GGCC_GC Cuts at: 2878 2878 Size: 5431 NspI r_CATG'y Cuts at: 1132 1229 2224 2789 4334 1132 Size: 97 995 565 1545 2229 Fragments arranged by size: 2229 1545 995 565 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5431 PciI A'CATG_T Cuts at: 2220 4330 2220 Size: 2110 3321 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5431 PfoI T'CCnGG_A Cuts at: 2042 2389 2042 Size: 347 5084 PmeI GTTT'AAAC Cuts at: 2735 2735 Size: 5431 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3500 3532 5013 5045 3500 Size: 32 1481 32 3886 Fragments arranged by size: 3886 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5431 PsiI TTA'TAA Cuts at: 3001 3001 Size: 5431 PstI C_TGCA'G Cuts at: 830 2783 830 Size: 1953 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5431 SalI G'TCGA_C Cuts at: 1936 2773 1936 Size: 837 4594 SbfI CC_TGCA'GG Cuts at: 2783 2783 Size: 5431 ScaI AGT'ACT Cuts at: 2574 3679 5391 2574 Size: 1105 1712 2614 Fragments arranged by size: 2614 1712 1105 SfcI C'TryA_G Cuts at: 826 1045 2779 3914 4595 4786 826 Size: 219 1734 1135 681 191 1471 Fragments arranged by size: 1734 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5431 SmaI CCC'GGG Cuts at: 1872 2758 1872 Size: 886 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3535 4436 4698 820 Size: 197 926 1592 901 262 1553 Fragments arranged by size: 1592 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3222 493 Size: 2729 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5431 SphI G_CATG'C Cuts at: 1132 2789 1132 Size: 1657 3774 SspI AAT'ATT Cuts at: 5 52 3355 5 Size: 47 3303 2081 Fragments arranged by size: 3303 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5431 StyI C'CwwG_G Cuts at: 513 1065 2870 513 Size: 552 1805 3074 Fragments arranged by size: 3074 1805 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3458 3475 3628 3813 4258 325 Size: 1255 1878 17 153 185 445 1498 Fragments arranged by size: 1878 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2294 2444 2572 Size: 274 80 33 51 1443 317 150 128 Cuts at: 2572 3677 5389 96 Size: 1105 1712 138 Fragments arranged by size: 1712 1443 1105 317 274 150 138 128 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2583 2615 5391 5423 2583 Size: 32 2776 32 2591 Fragments arranged by size: 2776 2591 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5431 XbaI T'CTAG_A Cuts at: 2767 2767 Size: 5431 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5431 XmaI C'CCGG_G Cuts at: 1870 2756 1870 Size: 886 4545 XmnI GAAnn'nnTTC Cuts at: 2205 3560 2205 Size: 1355 4076 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2585 3239 276 Size: 53 83 186 936 1051 654 2468 Fragments arranged by size: 2468 1051 936 654 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI