(Circular) (Six-base) MAPSORT of: pFN21AE0308.seq Check: 2714 from: 1 to: 6262 pFN21AE0308 6262 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4072 278 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3460 3605 1937 Size: 1523 145 4594 Fragments arranged by size: 4594 1523 145 Acc65I G'GTAC_C Cuts at: 2267 3583 2267 Size: 1316 4946 AclI AA'CG_TT Cuts at: 1563 4389 4762 1563 Size: 2826 373 3063 Fragments arranged by size: 3063 2826 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2438 2943 3398 4325 5709 5977 1632 Size: 806 505 455 927 1384 268 1917 Fragments arranged by size: 1917 1384 927 806 505 455 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6065 AflIII A'CryG_T Cuts at: 1203 5161 6138 1203 Size: 3958 977 1327 Fragments arranged by size: 3958 1327 977 AgeI A'CCGG_T Cuts at: 5093 5093 Size: 6262 AhdI GACnn_n'nnGTC Cuts at: 4991 4991 Size: 6262 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6262 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6262 AlwNI CAG_nnn'CTG Cuts at: 3019 3368 3377 3421 4005 5577 3019 Size: 349 9 44 584 1572 3704 Fragments arranged by size: 3704 1572 584 349 44 9 ApaI G_GGCC'C Cuts at: 2582 2582 Size: 6262 ApaLI G'TGCA_C Cuts at: 4319 5475 4319 Size: 1156 5106 ApoI r'AATT_y Cuts at: 1493 3507 3571 3797 3949 6058 1493 Size: 2014 64 226 152 2109 1697 Fragments arranged by size: 2109 2014 1697 226 152 64 AseI AT'TA_AT Cuts at: 160 1032 4816 160 Size: 872 3784 1606 Fragments arranged by size: 3784 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 AvaI C'yCGr_G Cuts at: 1870 1943 3587 1870 Size: 73 1644 4545 Fragments arranged by size: 4545 1644 73 BamHI G'GATC_C Cuts at: 2856 3592 3959 2856 Size: 736 367 5159 Fragments arranged by size: 5159 736 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2267 3583 5038 618 Size: 325 217 627 480 1316 1455 1842 Fragments arranged by size: 1842 1455 1316 627 480 325 217 BanII G_rGCy'C Cuts at: 1391 2582 2709 2737 2868 3581 1391 Size: 1191 127 28 131 713 4072 Fragments arranged by size: 4072 1191 713 131 127 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5291 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2394 2428 4453 4487 1903 Size: 34 457 34 2025 34 3678 Fragments arranged by size: 3678 2025 457 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4154 5364 4154 Size: 1210 5052 BclI T'GATC_A Cuts at: 1548 1548 Size: 6262 BfrBI ATG'CAT Cuts at: 2948 2948 Size: 6262 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4873 136 Size: 107 122 71 631 605 3201 1525 Fragments arranged by size: 3201 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6257 6257 Size: 6262 BlpI GC'TnA_GC Cuts at: 2651 3679 2651 Size: 1028 5234 Bme1580I G_kGCm'C Cuts at: 1790 2129 2582 4323 5479 1790 Size: 339 453 1741 1156 2573 Fragments arranged by size: 2573 1741 1156 453 339 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6262 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3993 4951 442 Size: 416 53 83 399 24 2576 958 1753 Fragments arranged by size: 2576 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6262 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2196 2196 Size: 6262 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2104 2168 2265 2563 2587 2771 4922 1239 Size: 865 64 97 298 24 184 2151 2579 Fragments arranged by size: 2579 2151 865 298 184 97 64 24 Bpu10I CC'TnA_GC Cuts at: 2505 2505 Size: 6262 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2792 4387 5252 5550 2792 Size: 1595 865 298 3504 Fragments arranged by size: 3504 1595 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4925 882 Size: 617 3426 2219 Fragments arranged by size: 3426 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4053 493 Size: 711 2849 2702 Fragments arranged by size: 2849 2702 711 BsaBI GATnn'nnATC Cuts at: 6256 6256 Size: 6262 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2095 4069 4451 5086 Size: 53 83 186 936 562 1974 382 635 Cuts at: 5086 275 Size: 1451 Fragments arranged by size: 1974 1451 936 635 562 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4695 5093 5367 5514 5925 4695 Size: 398 274 147 411 5032 Fragments arranged by size: 5032 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6037 Fragments arranged by size: 6037 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6262 BseYI C'CCAG_C Cuts at: 2122 2597 5465 5961 2122 Size: 475 2868 496 2423 Fragments arranged by size: 2868 2423 496 475 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5689 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3712 4473 5501 1540 Size: 275 186 1711 761 1028 2301 Fragments arranged by size: 2301 1711 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2778 4323 4408 5479 2778 Size: 1545 85 1071 3561 Fragments arranged by size: 3561 1545 1071 85 BsmI GAATG_Cn' Cuts at: 2679 3773 3866 2679 Size: 1094 93 5075 Fragments arranged by size: 5075 1094 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 2582 2709 2737 2778 2868 3581 Size: 399 339 453 127 28 41 90 713 Cuts at: 3581 4323 4408 5479 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 713 453 399 339 127 90 85 41 28 BspEI T'CCGG_A Cuts at: 5925 5925 Size: 6262 BspHI T'CATG_A Cuts at: 3088 4151 5881 3088 Size: 1063 1730 3469 Fragments arranged by size: 3469 1730 1063 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3617 844 Size: 2773 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2730 4149 1394 Size: 429 907 1419 3507 Fragments arranged by size: 3507 1419 907 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2302 4757 4931 65 Size: 1183 1054 2455 174 1396 Fragments arranged by size: 2455 1396 1183 1054 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2091 2356 4906 5093 1700 Size: 110 281 265 2550 187 2869 Fragments arranged by size: 2869 2550 281 265 187 110 BsrGI T'GTAC_A Cuts at: 96 2325 3190 96 Size: 2229 865 3168 Fragments arranged by size: 3168 2229 865 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6262 BssSI C'ACGA_G Cuts at: 4322 5334 4322 Size: 1012 5250 BstAPI GCAn_nnn'nTGC Cuts at: 794 2417 794 Size: 1623 4639 BstBI TT'CG_AA Cuts at: 5066 5066 Size: 6262 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6262 BstXI CCAn_nnnn'nTGG Cuts at: 2146 2297 2146 Size: 151 6111 BstYI r'GATC_y Cuts at: 1972 2622 2856 3592 3959 4344 4361 5813 5899 Size: 650 234 736 367 385 17 1452 86 Cuts at: 5899 5911 6257 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 736 650 385 367 346 234 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2066 2368 513 Size: 552 1001 302 4407 Fragments arranged by size: 4407 1001 552 302 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2201 487 Size: 683 56 975 4548 Fragments arranged by size: 4548 975 683 56 BtsI GCAGTG_nn' Cuts at: 2127 2412 2703 3778 4572 4592 2127 Size: 285 291 1075 794 20 3797 Fragments arranged by size: 3797 1075 794 291 285 20 ClaI AT'CG_AT Cuts at: 1554 3483 1554 Size: 1929 4333 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2350 2385 529 Size: 35 1786 35 4406 Fragments arranged by size: 4406 1786 35 35 DraI TTT'AAA Cuts at: 3566 3566 Size: 6262 DraIII CAC_nnn'GTG Cuts at: 2133 2133 Size: 6262 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2881 5269 809 Size: 507 1565 2388 1802 Fragments arranged by size: 2388 1802 1565 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2358 2917 3043 Size: 54 1425 50 275 18 528 559 126 Cuts at: 3043 3709 4598 6227 8 Size: 666 889 1629 43 Fragments arranged by size: 1629 1425 889 666 559 528 275 126 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3709 1537 Size: 275 1897 4090 Fragments arranged by size: 4090 1897 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3380 4192 1353 Size: 2027 812 3423 Fragments arranged by size: 3423 2027 812 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2702 4849 5223 5369 387 Size: 1064 138 1113 2147 374 146 1280 Fragments arranged by size: 2147 1280 1113 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2104 2168 2265 2438 2563 2587 2771 Size: 393 472 64 97 173 125 24 184 Cuts at: 2771 2943 3398 4325 4922 5709 5977 1239 Size: 172 455 927 597 787 268 1524 Fragments arranged by size: 1524 927 787 597 472 455 393 268 184 173 172 125 97 64 24 EcoNI CCTnn'n_nnAGG Cuts at: 3063 3063 Size: 6262 EcoO109I rG'GnC_Cy Cuts at: 2103 2175 2578 2874 2103 Size: 72 403 296 5491 Fragments arranged by size: 5491 403 296 72 EcoRI G'AATT_C Cuts at: 3571 3949 3571 Size: 378 5884 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2833 3319 2833 Size: 486 5776 FspI TGC'GCA Cuts at: 4768 4768 Size: 6262 HaeII r_GCGC'y Cuts at: 1678 2640 2772 5409 1678 Size: 962 132 2637 2531 Fragments arranged by size: 2637 2531 962 132 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3121 3153 4903 4935 4977 Size: 32 135 32 1549 32 1750 32 42 Cuts at: 4977 5009 1373 Size: 32 2626 Fragments arranged by size: 2626 1750 1549 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3606 3852 669 Size: 988 281 1668 246 3079 Fragments arranged by size: 3079 1668 988 281 246 HindIII A'AGCT_T Cuts at: 748 3476 748 Size: 2728 3534 HpaI GTT'AAC Cuts at: 3852 3852 Size: 6262 KpnI G_GTAC'C Cuts at: 2271 3587 2271 Size: 1316 4946 MfeI C'AATT_G Cuts at: 3861 3861 Size: 6262 MluI A'CGCG_T Cuts at: 6138 6138 Size: 6262 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2422 5376 5560 1305 Size: 1117 2954 184 2007 Fragments arranged by size: 2954 2007 1117 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4440 Fragments arranged by size: 4440 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3087 4220 4579 4738 518 Size: 648 1921 1133 359 159 2042 Fragments arranged by size: 2042 1921 1133 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2068 2781 3678 4355 5503 5748 1606 Size: 462 713 897 677 1148 245 2120 Fragments arranged by size: 2120 1148 897 713 677 462 245 NaeI GCC'GGC Cuts at: 2093 2358 2093 Size: 265 5997 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5710 NdeI CA'TA_TG Cuts at: 387 387 Size: 6262 NgoMIV G'CCGG_C Cuts at: 2091 2356 2091 Size: 265 5997 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6262 NotI GC'GGCC_GC Cuts at: 3709 3709 Size: 6262 NsiI A_TGCA'T Cuts at: 2950 2950 Size: 6262 NspI r_CATG'y Cuts at: 1132 1229 2405 3620 5165 1132 Size: 97 1176 1215 1545 2229 Fragments arranged by size: 2229 1545 1215 1176 97 PasI CC'CwG_GG Cuts at: 1348 2290 2423 1348 Size: 942 133 5187 Fragments arranged by size: 5187 942 133 PciI A'CATG_T Cuts at: 5161 5161 Size: 6262 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6262 PfoI T'CCnGG_A Cuts at: 2560 2560 Size: 6262 PmeI GTTT'AAAC Cuts at: 3566 3566 Size: 6262 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4331 4363 5844 5876 4331 Size: 32 1481 32 4717 Fragments arranged by size: 4717 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2103 2874 2103 Size: 771 5491 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6262 PsiI TTA'TAA Cuts at: 3135 3832 3135 Size: 697 5565 PspOMI G'GGCC_C Cuts at: 2578 2578 Size: 6262 PstI C_TGCA'G Cuts at: 830 2313 2595 3373 3614 830 Size: 1483 282 778 241 3478 Fragments arranged by size: 3478 1483 778 282 241 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6262 SacII CC_GC'GG Cuts at: 2069 2069 Size: 6262 SalI G'TCGA_C Cuts at: 1936 3604 1936 Size: 1668 4594 SapI GCTCTTCn'nnn_ Cuts at: 3380 3380 Size: 6262 SbfI CC_TGCA'GG Cuts at: 3614 3614 Size: 6262 ScaI AGT'ACT Cuts at: 4510 6222 4510 Size: 1712 4550 SexAI A'CCwGG_T Cuts at: 2517 2517 Size: 6262 SfcI C'TryA_G Cuts at: 826 1045 2309 2487 2591 2626 3369 3610 4745 Size: 219 1264 178 104 35 743 241 1135 Cuts at: 4745 5426 5617 826 Size: 681 191 1471 Fragments arranged by size: 1471 1264 1135 743 681 241 219 191 178 104 35 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6262 SmaI CCC'GGG Cuts at: 1872 3589 1872 Size: 1717 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2807 4366 5267 5529 820 Size: 197 926 864 1559 901 262 1553 Fragments arranged by size: 1559 1553 926 901 864 262 197 SnaBI TAC'GTA Cuts at: 493 4053 493 Size: 3560 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6262 SphI G_CATG'C Cuts at: 1132 2405 3620 1132 Size: 1273 1215 3774 Fragments arranged by size: 3774 1273 1215 SspI AAT'ATT Cuts at: 5 52 4186 5 Size: 47 4134 2081 Fragments arranged by size: 4134 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6262 StyI C'CwwG_G Cuts at: 513 1065 2099 2817 3223 3269 3701 513 Size: 552 1034 718 406 46 432 3074 Fragments arranged by size: 3074 1034 718 552 432 406 46 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2990 4289 4306 4459 4644 5089 325 Size: 1255 1410 1299 17 153 185 445 1498 Fragments arranged by size: 1498 1410 1299 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2325 3071 3190 Size: 274 80 33 51 1443 348 746 119 Cuts at: 3190 4508 6220 96 Size: 1318 1712 138 Fragments arranged by size: 1712 1443 1318 746 348 274 138 119 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3234 3266 6222 6254 3234 Size: 32 2956 32 3242 Fragments arranged by size: 3242 2956 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6262 XbaI T'CTAG_A Cuts at: 3598 3598 Size: 6262 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6262 XmaI C'CCGG_G Cuts at: 1870 3587 1870 Size: 1717 4545 XmnI GAAnn'nnTTC Cuts at: 4391 4391 Size: 6262 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4070 276 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SanDI SfiI SfoI SrfI SwaI XcmI