Restriction map for
pFN21AE0308
(Linear) (Six-base) MAPSORT of: KIEE0308.seq Check: 9448 from: 1 to: 1557
KIEE0308.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 0 1455 1557
Size: 1455 102
Acc65I G'GTAC_C
Cuts at: 0 262 1557
Size: 262 1295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 938 1393 1557
Size: 433 505 455 164
Fragments arranged by size:
505 455 433 164
AlwNI CAG_nnn'CTG
Cuts at: 0 1014 1363 1372 1416 1557
Size: 1014 349 9 44 141
Fragments arranged by size:
1014 349 141 44 9
ApaI G_GGCC'C
Cuts at: 0 577 1557
Size: 577 980
ApoI r'AATT_y
Cuts at: 0 1502 1557
Size: 1502 55
BamHI G'GATC_C
Cuts at: 0 851 1557
Size: 851 706
BanI G'GyrC_C
Cuts at: 0 262 1557
Size: 262 1295
BanII G_rGCy'C
Cuts at: 0 577 704 732 863 1557
Size: 577 127 28 131 694
Fragments arranged by size:
694 577 131 127 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 389 423 1557
Size: 389 34 1134
Fragments arranged by size:
1134 389 34
BfrBI ATG'CAT
Cuts at: 0 943 1557
Size: 943 614
BlpI GC'TnA_GC
Cuts at: 0 646 1557
Size: 646 911
Bme1580I G_kGCm'C
Cuts at: 0 124 577 1557
Size: 124 453 980
Fragments arranged by size:
980 453 124
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 191 1557
Size: 191 1366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 163 260 558 582 766 1557
Size: 99 64 97 298 24 184 791
Fragments arranged by size:
791 298 184 99 97 64 24
Bpu10I CC'TnA_GC
Cuts at: 0 500 1557
Size: 500 1057
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 1557
Size: 787 770
BsaHI Gr'CG_yC
Cuts at: 0 90 1557
Size: 90 1467
BseYI C'CCAG_C
Cuts at: 0 117 592 1557
Size: 117 475 965
Fragments arranged by size:
965 475 117
BsiHKAI G_wGCw'C
Cuts at: 0 773 1557
Size: 773 784
BsmI GAATG_Cn'
Cuts at: 0 674 1557
Size: 674 883
Bsp1286I G_dGCh'C
Cuts at: 0 124 577 704 732 773 863 1557
Size: 124 453 127 28 41 90 694
Fragments arranged by size:
694 453 127 124 90 41 28
BspHI T'CATG_A
Cuts at: 0 1083 1557
Size: 1083 474
BsrBI CCG'CTC
Cuts at: 0 725 1557
Size: 725 832
BsrDI GCAATG_nn'
Cuts at: 0 297 1557
Size: 297 1260
BsrFI r'CCGG_y
Cuts at: 0 86 351 1557
Size: 86 265 1206
Fragments arranged by size:
1206 265 86
BsrGI T'GTAC_A
Cuts at: 0 320 1185 1557
Size: 320 865 372
Fragments arranged by size:
865 372 320
BstAPI GCAn_nnn'nTGC
Cuts at: 0 412 1557
Size: 412 1145
BstXI CCAn_nnnn'nTGG
Cuts at: 0 141 292 1557
Size: 141 151 1265
Fragments arranged by size:
1265 151 141
BstYI r'GATC_y
Cuts at: 0 617 851 1557
Size: 617 234 706
Fragments arranged by size:
706 617 234
BtgI C'CryG_G
Cuts at: 0 61 363 1557
Size: 61 302 1194
Fragments arranged by size:
1194 302 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 196 1557
Size: 196 1361
BtsI GCAGTG_nn'
Cuts at: 0 122 407 698 1557
Size: 122 285 291 859
Fragments arranged by size:
859 291 285 122
ClaI AT'CG_AT
Cuts at: 0 1478 1557
Size: 1478 79
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 345 380 1557
Size: 345 35 1177
Fragments arranged by size:
1177 345 35
DraIII CAC_nnn'GTG
Cuts at: 0 128 1557
Size: 128 1429
DrdI GACnn_nn'nnGTC
Cuts at: 0 876 1557
Size: 876 681
EaeI y'GGCC_r
Cuts at: 0 353 912 1038 1557
Size: 353 559 126 519
Fragments arranged by size:
559 519 353 126
EarI CTCTTCn'nnn_
Cuts at: 0 1375 1557
Size: 1375 182
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 697 1557
Size: 697 860
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 163 260 433 558 582 766 938
Size: 99 64 97 173 125 24 184 172
Cuts at: 938 1393 1557
Size: 455 164
Fragments arranged by size:
455 184 173 172 164 125 99 97
64 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1058 1557
Size: 1058 499
EcoO109I rG'GnC_Cy
Cuts at: 0 98 170 573 869 1557
Size: 98 72 403 296 688
Fragments arranged by size:
688 403 296 98 72
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 828 1314 1557
Size: 828 486 243
Fragments arranged by size:
828 486 243
HaeII r_GCGC'y
Cuts at: 0 635 767 1557
Size: 635 132 790
Fragments arranged by size:
790 635 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1116 1148 1557
Size: 1116 32 409
Fragments arranged by size:
1116 409 32
HindIII A'AGCT_T
Cuts at: 0 1471 1557
Size: 1471 86
KpnI G_GTAC'C
Cuts at: 0 266 1557
Size: 266 1291
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 417 1557
Size: 417 1140
MslI CAynn'nnrTG
Cuts at: 0 1082 1557
Size: 1082 475
MspA1I CmG'CkG
Cuts at: 0 63 776 1557
Size: 63 713 781
Fragments arranged by size:
781 713 63
NaeI GCC'GGC
Cuts at: 0 88 353 1557
Size: 88 265 1204
Fragments arranged by size:
1204 265 88
NgoMIV G'CCGG_C
Cuts at: 0 86 351 1557
Size: 86 265 1206
Fragments arranged by size:
1206 265 86
NsiI A_TGCA'T
Cuts at: 0 945 1557
Size: 945 612
NspI r_CATG'y
Cuts at: 0 400 1557
Size: 400 1157
PasI CC'CwG_GG
Cuts at: 0 285 418 1557
Size: 285 133 1139
Fragments arranged by size:
1139 285 133
PfoI T'CCnGG_A
Cuts at: 0 555 1557
Size: 555 1002
PpuMI rG'GwC_Cy
Cuts at: 0 98 869 1557
Size: 98 771 688
Fragments arranged by size:
771 688 98
PsiI TTA'TAA
Cuts at: 0 1130 1557
Size: 1130 427
PspOMI G'GGCC_C
Cuts at: 0 573 1557
Size: 573 984
PstI C_TGCA'G
Cuts at: 0 308 590 1368 1557
Size: 308 282 778 189
Fragments arranged by size:
778 308 282 189
SacII CC_GC'GG
Cuts at: 0 64 1557
Size: 64 1493
SapI GCTCTTCn'nnn_
Cuts at: 0 1375 1557
Size: 1375 182
SexAI A'CCwGG_T
Cuts at: 0 512 1557
Size: 512 1045
SfcI C'TryA_G
Cuts at: 0 304 482 586 621 1364 1557
Size: 304 178 104 35 743 193
Fragments arranged by size:
743 304 193 178 104 35
SmlI C'TyrA_G
Cuts at: 0 802 1557
Size: 802 755
SphI G_CATG'C
Cuts at: 0 400 1557
Size: 400 1157
StyI C'CwwG_G
Cuts at: 0 94 812 1218 1264 1557
Size: 94 718 406 46 293
Fragments arranged by size:
718 406 293 94 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 985 1557
Size: 985 572
TatI w'GTAC_w
Cuts at: 0 320 1066 1185 1557
Size: 320 746 119 372
Fragments arranged by size:
746 372 320 119
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1229 1261 1557
Size: 1229 32 296
Fragments arranged by size:
1229 296 32
Enzymes that do cut:
AccI Acc65I AcuI AlwNI ApaI ApoI BamHI BanI
BanII BcgI BfrBI BlpI Bme1580I BplI BpmI Bpu10I
BpuEI BsaHI BseYI BsiHKAI BsmI Bsp1286I BspHI BsrBI
BsrDI BsrFI BsrGI BstAPI BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EarI EciI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII KpnI
MmeI MslI MspA1I NaeI NgoMIV NsiI NspI PasI
PfoI PpuMI PsiI PspOMI PstI SacII SapI SexAI
SfcI SmlI SphI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbsI BbvCI BciVI BclI BglI
BglII BmgBI BmrI BmtI BsaI BsaAI BsaBI BsaWI
BsaXI BseRI BsgI BsiEI BsiWI BsmBI BspEI BspMI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I DraI EagI
EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HpaI
KasI MfeI MluI MscI NarI NcoI NdeI NheI
NotI NruI PacI PciI PflMI PmeI PmlI PpiI
PshAI PspXI PsrI PvuI PvuII RsrII SacI SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI