################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BclI BfaI BfrI BlnI BpuAI BsaAI BsaBI BsaI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 981 TCCGGA T^CCGGA AflIII 768 ACGCGT A^C[GA][CT]GT AlwI 591 GATCC GGATC(4/5) ApaI 387 GGGCCC GGGCC^C BalI 581 TGGCCA TGG^CCA BciVI 1030 GTATCC GTATCC(6/5) BglII 617 AGATCT A^GATCT BlpI 748 GCTCAGC GC^T[ACGT]AGC Bpu1102I 748 GCTCAGC GC^T[ACGT]AGC BsaWI 981 TCCGGA [AT]^CCGG[AT] BseAI 981 TCCGGA T^CCGGA BseRI 1053 CTCCTC GAGGAG(10/8) BsiEI 1101 CGGTCG CG[GA][CT]^CG BsmAI 978 GTCTC GTCTC(1/5) BspEI 981 TCCGGA T^CCGGA BsrI 975 CCAGT ACTGG(1/-1) BssHII 473 GCGCGC G^CGCGC BstF5I 334 GGATG GGATG(2/0) BstXI 908 CCATCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 617 AGATCT [GA]^GATC[CT] CelII 748 GCTCAGC GC^T[ACGT]AGC EarI 499 CTCTTC CTCTTC(1/4) FokI 334 GGATG GGATG(9/13) Ksp632I 499 CTCTTC CTCTTC(1/4) KspI 1010 CCGCGG CCGC^GG MaeII 443 ACGT A^CGT MaeIII 190 GTCAC ^GT[ACGT]AC MboII 500 TCTTC GAAGA(8/7) MflI 617 AGATCT [GA]^GATC[CT] MluI 768 ACGCGT A^CGCGT MluNI 581 TGGCCA TGG^CCA MroI 981 TCCGGA T^CCGGA MscI 581 TGGCCA TGG^CCA NaeI 931 GCCGGC GCC^GGC NgoMIV 931 GCCGGC G^CCGGC SacII 1010 CCGCGG CCGC^GG SapI 498 GCTCTTC GCTCTTC(1/4) SmaI 948 CCCGGG CCC^GGG SmlI 48 CTTGAG C^T[CT][GA]AG TaqI 4 TCGA T^CGA Tsp45I 190 GTCAC ^GT[GC]AC TspRI 35 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 4 TCGA T^CGA XhoII 617 AGATCT [GA]^GATC[CT] XmaCI 948 CCCGGG C^CCGGG XmaI 948 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwNI 35 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG 679 CAGCAACTG AspHI 496 GTGCTC G[AT]GC[AT]^C 574 GAGCAC BglI 842 GCCTACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 886 GCCGCGCAGGC BpmI 58 CTGGAG CTGGAG(16/14) 526 CTGGAG BsiHKAI 496 GTGCTC G[AT]GC[AT]^C 574 GAGCAC BspMI 513 GCAGGT ACCTGC(4/8) 609 GCAGGT BsrBI 396 GAGCGG CCGCTC(-3/-3) 411 GAGCGG BsrFI 780 GCCGGT [GA]^CCGG[CT] 931 GCCGGC Cfr10I 780 GCCGGT [GA]^CCGG[CT] 931 GCCGGC DdeI 33 CTCAG C^T[ACGT]AG 749 CTCAG DpnII 591 GATC ^GATC 618 GATC DraIII 166 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG 723 CACCGGGTG EcoT14I 659 CCAAGG C^C[AT][AT]GG 936 CCTTGG HphI 789 GGTGA GGTGA(8/7) 191 TCACC MboI 591 GATC ^GATC 618 GATC MslI 402 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 909 CATCTCCGTG NdeII 591 GATC ^GATC 618 GATC PstI 685 CTGCAG CTGCA^G 1018 CTGCAG Sau3AI 591 GATC ^GATC 618 GATC SfcI 685 CTGCAG C^T[GA][CT]AG 1018 CTGCAG StyI 659 CCAAGG C^C[AT][AT]GG 936 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaI AvaII BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BssKI BstAPI BstNI BstUI Cac8I CfoI Cfr13I DsaI EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI KasI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIII NlaIV PleI RsaI Sau96I ScrFI SfoI TseI