Restriction map for
pFN21AB8975
(Circular) (Six-base) MAPSORT of: pFN21AB8975.seq Check: 8929 from: 1 to: 5818
pFN21AB8975 5818 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2509 2509
Size: 5818
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3628 278
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3161 1937
Size: 1224 4594
Acc65I G'GTAC_C
Cuts at: 3139 3139
Size: 5818
AclI AA'CG_TT
Cuts at: 1563 3945 4318 1563
Size: 2382 373 3063
Fragments arranged by size:
3063 2382 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3881 5265 5533 1632
Size: 2249 1384 268 1917
Fragments arranged by size:
2249 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5621
AflIII A'CryG_T
Cuts at: 1203 2773 4717 5694 1203
Size: 1570 1944 977 1327
Fragments arranged by size:
1944 1570 1327 977
AgeI A'CCGG_T
Cuts at: 4649 4649
Size: 5818
AhdI GACnn_n'nnGTC
Cuts at: 4547 4547
Size: 5818
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5818
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5818
AlwNI CAG_nnn'CTG
Cuts at: 2045 2689 3561 5133 2045
Size: 644 872 1572 2730
Fragments arranged by size:
2730 1572 872 644
ApaI G_GGCC'C
Cuts at: 2396 2396
Size: 5818
ApaLI G'TGCA_C
Cuts at: 3875 5031 3875
Size: 1156 4662
ApoI r'AATT_y
Cuts at: 1493 3127 3353 3505 5614 1493
Size: 1634 226 152 2109 1697
Fragments arranged by size:
2109 1697 1634 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4372 160
Size: 872 3340 1606
Fragments arranged by size:
3340 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
AvaI C'yCGr_G
Cuts at: 1870 1943 2105 2953 3061 3143 1870
Size: 73 162 848 108 82 4545
Fragments arranged by size:
4545 848 162 108 82 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2540 2573 2540
Size: 33 5785
BamHI G'GATC_C
Cuts at: 3148 3515 3148
Size: 367 5451
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2143 2181 2189 2227 2348
Size: 325 217 627 356 38 8 38 121
Cuts at: 2348 2566 2726 2733 2782 2822 2837 2933 3077
Size: 218 160 7 49 40 15 96 144
Cuts at: 3077 3139 4594 618
Size: 62 1455 1842
Fragments arranged by size:
1842 1455 627 356 325 218 217 160
144 121 96 62 49 40 38 38
15 8 7
BanII G_rGCy'C
Cuts at: 1391 2113 2396 2438 3068 3137 1391
Size: 722 283 42 630 69 4072
Fragments arranged by size:
4072 722 630 283 69 42
BbeI G_GCGC'C
Cuts at: 2193 2352 2570 2841 2937 2193
Size: 159 218 271 96 5074
Fragments arranged by size:
5074 271 218 159 96
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4847
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4009 4043 1903
Size: 34 2072 34 3678
Fragments arranged by size:
3678 2072 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3046 3710 4920 3046
Size: 664 1210 3944
Fragments arranged by size:
3944 1210 664
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5818
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2853 2897 4429
Size: 107 122 71 631 605 1181 44 1532
Cuts at: 4429 136
Size: 1525
Fragments arranged by size:
1532 1525 1181 631 605 122 107 71
44
BglII A'GATC_T
Cuts at: 2622 5813 2622
Size: 3191 2627
BlpI GC'TnA_GC
Cuts at: 2754 3235 2754
Size: 481 5337
Bme1580I G_kGCm'C
Cuts at: 1790 2184 2232 2396 2729 2738 2827 3879 5035
Size: 394 48 164 333 9 89 1052 1156
Cuts at: 5035 1790
Size: 2573
Fragments arranged by size:
2573 1156 1052 394 333 164 89 48
9
BmgBI CAC'GTC
Cuts at: 1318 2449 1318
Size: 1131 4687
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3549 4507 442
Size: 416 53 83 399 24 2132 958 1753
Fragments arranged by size:
2132 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2552 4478 1239
Size: 845 468 1926 2579
Fragments arranged by size:
2579 1926 845 468
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2074 3943 4808 5106 2074
Size: 1869 865 298 2786
Fragments arranged by size:
2786 1869 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4481 882
Size: 617 2982 2219
Fragments arranged by size:
2982 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3609 493
Size: 711 2405 2702
Fragments arranged by size:
2702 2405 711
BsaBI GATnn'nnATC
Cuts at: 5812 5812
Size: 5818
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2190 2349 2567 2606
Size: 53 83 186 936 657 159 218 39
Cuts at: 2606 2838 2934 3625 4007 4642 275
Size: 232 96 691 382 635 1451
Fragments arranged by size:
1451 936 691 657 635 382 232 218
186 159 96 83 53 39
BsaWI w'CCGG_w
Cuts at: 2986 4251 4649 4923 5070 5481 2986
Size: 1265 398 274 147 411 3323
Fragments arranged by size:
3323 1265 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3041 3071 1178
Size: 30 165 30 1638 30 3925
Fragments arranged by size:
3925 1638 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3049 1186
Size: 1863 3955
BseYI C'CCAG_C
Cuts at: 2232 2440 5021 5517 2232
Size: 208 2581 496 2533
Fragments arranged by size:
2581 2533 496 208
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5245
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3268 4029 5057 1540
Size: 275 186 1108 159 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 275 186 159
BsiHKAI G_wGCw'C
Cuts at: 2505 2583 3879 3964 5035 2505
Size: 78 1296 85 1071 3288
Fragments arranged by size:
3288 1296 1071 85 78
BsmI GAATG_Cn'
Cuts at: 3329 3422 3329
Size: 93 5725
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2113 2184 2232 2396 2438 2505 2583
Size: 399 323 71 48 164 42 67 78
Cuts at: 2583 2729 2738 2827 3068 3137 3879 3964 5035
Size: 146 9 89 241 69 742 85 1071
Cuts at: 5035 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 323 241 164 146
89 85 78 71 69 67 48 42
9
BspEI T'CCGG_A
Cuts at: 2986 5481 2986
Size: 2495 3323
BspHI T'CATG_A
Cuts at: 3707 5437 3707
Size: 1730 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2509 2605 3173 844
Size: 1665 96 568 3489
Fragments arranged by size:
3489 1665 568 96
BsrBI CCG'CTC
Cuts at: 1394 1823 2403 2418 3705 1394
Size: 429 580 15 1287 3507
Fragments arranged by size:
3507 1287 580 429 15
BsrDI GCAATG_nn'
Cuts at: 65 1248 4313 4487 65
Size: 1183 3065 174 1396
Fragments arranged by size:
3065 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2785 2936 4462 4649 1700
Size: 110 975 151 1526 187 2869
Fragments arranged by size:
2869 1526 975 187 151 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5818
BssHII G'CGCG_C
Cuts at: 1925 2478 1925
Size: 553 5265
BssSI C'ACGA_G
Cuts at: 3878 4890 3878
Size: 1012 4806
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2689 2733 2811 794
Size: 1895 44 78 3801
Fragments arranged by size:
3801 1895 78 44
BstBI TT'CG_AA
Cuts at: 4622 4622
Size: 5818
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5818
BstXI CCAn_nnnn'nTGG
Cuts at: 2920 2920
Size: 5818
BstYI r'GATC_y
Cuts at: 1972 2622 3148 3515 3900 3917 5369 5455 5467
Size: 650 526 367 385 17 1452 86 12
Cuts at: 5467 5813 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 650 526 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2175 2185 2250 2919 3015 513
Size: 552 1110 10 65 669 96 3316
Fragments arranged by size:
3316 1110 669 552 96 65 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5079
Fragments arranged by size:
5079 683 56
BtsI GCAGTG_nn'
Cuts at: 3334 4128 4148 3334
Size: 794 20 5004
Fragments arranged by size:
5004 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5818
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5783
DraI TTT'AAA
Cuts at: 3122 3122
Size: 5818
DraIII CAC_nnn'GTG
Cuts at: 2176 2733 2176
Size: 557 5261
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4825 809
Size: 507 3509 1802
Fragments arranged by size:
3509 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 2586 2910
Size: 54 1425 50 275 18 327 429 324
Cuts at: 2910 3265 4154 5783 8
Size: 355 889 1629 43
Fragments arranged by size:
1629 1425 889 429 355 327 324 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3265 1537
Size: 275 1453 4090
Fragments arranged by size:
4090 1453 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2510 3748 1353
Size: 1157 1238 3423
Fragments arranged by size:
3423 1238 1157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2236 4405 4779 4925 387
Size: 1064 138 647 2169 374 146 1280
Fragments arranged by size:
2169 1280 1064 647 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2552 3881 4478 5265 5533 1239
Size: 393 452 468 1329 597 787 268 1524
Fragments arranged by size:
1524 1329 787 597 468 452 393 268
EcoRI G'AATT_C
Cuts at: 3127 3505 3127
Size: 378 5440
FspI TGC'GCA
Cuts at: 4324 4324
Size: 5818
HaeII r_GCGC'y
Cuts at: 1678 2193 2328 2352 2497 2570 2659 2841 2937
Size: 515 135 24 145 73 89 182 96
Cuts at: 2937 3023 4965 1678
Size: 86 1942 2531
Fragments arranged by size:
2531 1942 515 182 145 135 96 89
86 73 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4459 4491 4533 4565 1373
Size: 32 135 32 2887 32 42 32 2626
Fragments arranged by size:
2887 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3162 3408 669
Size: 988 281 1224 246 3079
Fragments arranged by size:
3079 1224 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5818
HpaI GTT'AAC
Cuts at: 3408 3408
Size: 5818
KasI G'GCGC_C
Cuts at: 2189 2348 2566 2837 2933 2189
Size: 159 218 271 96 5074
Fragments arranged by size:
5074 271 218 159 96
KpnI G_GTAC'C
Cuts at: 3143 3143
Size: 5818
MfeI C'AATT_G
Cuts at: 3417 3417
Size: 5818
MluI A'CGCG_T
Cuts at: 2773 5694 2773
Size: 2921 2897
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3089 4932 5116 1305
Size: 1784 1843 184 2007
Fragments arranged by size:
2007 1843 1784 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2588 10
Size: 54 1425 343 756 3240
Fragments arranged by size:
3240 1425 756 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2411 2918 3776 4135 4294 518
Size: 648 843 402 507 858 359 159 2042
Fragments arranged by size:
2042 858 843 648 507 402 359 159
MspA1I CmG'CkG
Cuts at: 1606 2116 2156 2470 2703 2909 2928 3017 3234
Size: 510 40 314 233 206 19 89 217
Cuts at: 3234 3911 5059 5304 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 510 314 245 233 217
206 89 40 19
NaeI GCC'GGC
Cuts at: 2938 2938
Size: 5818
NarI GG'CG_CC
Cuts at: 2190 2349 2567 2838 2934 2190
Size: 159 218 271 96 5074
Fragments arranged by size:
5074 271 218 159 96
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5266
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5818
NgoMIV G'CCGG_C
Cuts at: 2936 2936
Size: 5818
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5818
NotI GC'GGCC_GC
Cuts at: 3265 3265
Size: 5818
NspI r_CATG'y
Cuts at: 1132 1229 3176 4721 1132
Size: 97 1947 1545 2229
Fragments arranged by size:
2229 1947 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5818
PciI A'CATG_T
Cuts at: 4717 4717
Size: 5818
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5818
PmeI GTTT'AAAC
Cuts at: 3122 3122
Size: 5818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2312 2344 3887 3919 5400 5432 2312
Size: 32 1543 32 1481 32 2698
Fragments arranged by size:
2698 1543 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5818
PsiI TTA'TAA
Cuts at: 3388 3388
Size: 5818
PspOMI G'GGCC_C
Cuts at: 2392 2392
Size: 5818
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2627 2659 2627
Size: 32 5786
PstI C_TGCA'G
Cuts at: 830 2694 3027 3170 830
Size: 1864 333 143 3478
Fragments arranged by size:
3478 1864 333 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5818
SacII CC_GC'GG
Cuts at: 3018 3018
Size: 5818
SalI G'TCGA_C
Cuts at: 1936 3160 1936
Size: 1224 4594
SapI GCTCTTCn'nnn_
Cuts at: 2510 2510
Size: 5818
SbfI CC_TGCA'GG
Cuts at: 3170 3170
Size: 5818
ScaI AGT'ACT
Cuts at: 4066 5778 4066
Size: 1712 4106
SfcI C'TryA_G
Cuts at: 826 1045 2690 3023 3166 4301 4982 5173 826
Size: 219 1645 333 143 1135 681 191 1471
Fragments arranged by size:
1645 1471 1135 681 333 219 191 143
SfoI GGC'GCC
Cuts at: 2191 2350 2568 2839 2935 2191
Size: 159 218 271 96 5074
Fragments arranged by size:
5074 271 218 159 96
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5818
SmaI CCC'GGG
Cuts at: 1872 2955 3145 1872
Size: 1083 190 4545
Fragments arranged by size:
4545 1083 190
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2053 3922 4823 5085 820
Size: 197 926 110 1869 901 262 1553
Fragments arranged by size:
1869 1553 926 901 262 197 110
SnaBI TAC'GTA
Cuts at: 493 3609 493
Size: 3116 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5818
SphI G_CATG'C
Cuts at: 1132 3176 1132
Size: 2044 3774
SspI AAT'ATT
Cuts at: 5 52 3742 5
Size: 47 3690 2081
Fragments arranged by size:
3690 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5818
StyI C'CwwG_G
Cuts at: 513 1065 2664 2941 3257 513
Size: 552 1599 277 316 3074
Fragments arranged by size:
3074 1599 552 316 277
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3845 3862 4015 4200 4645 325
Size: 1255 2265 17 153 185 445 1498
Fragments arranged by size:
2265 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4064 5776 96
Size: 274 80 33 51 1443 2087 1712 138
Fragments arranged by size:
2087 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3041 3073 5778 5810 3041
Size: 32 2705 32 3049
Fragments arranged by size:
3049 2705 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5818
XbaI T'CTAG_A
Cuts at: 3154 3154
Size: 5818
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5818
XmaI C'CCGG_G
Cuts at: 1870 2953 3143 1870
Size: 1083 190 4545
Fragments arranged by size:
4545 1083 190
XmnI GAAnn'nnTTC
Cuts at: 3947 3947
Size: 5818
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3626 276
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspXI PvuII RsrII SacI SanDI SexAI SfiI
SrfI SwaI XcmI