################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## Acc65I AccI AclI AfaI AflII AflIII AgeI AhdI AlwNI AocI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MluNI MscI MseI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI ScaI SexAI SfiI SfuI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AatII 494 GACGTC GACGT^C AccIII 635 TCCGGA T^CCGGA AcsI 520 GAATTC [GA]^AATT[CT] Alw44I 148 GTGCAC G^TGCAC AlwI 388 GATCC GGATC(4/5) Aor51HI 327 AGCGCT AGC^GCT ApaI 414 GGGCCC GGGCC^C ApaLI 148 GTGCAC G^TGCAC ApoI 520 GAATTC [GA]^AATT[CT] AvaI 298 CCCGAG C^[CT]CG[GA]G AvaII 105 GGACC G^G[AT]CC BbeI 21 GGCGCC GGCGC^C BglI 358 GCCAACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 656 GAGACC GGTCTC(1/5) BsaWI 635 TCCGGA [AT]^CCGG[AT] BseAI 635 TCCGGA T^CCGGA BseRI 448 CTCCTC GAGGAG(10/8) BsiEI 402 CGGTCG CG[GA][CT]^CG BsmFI 492 GGGAC GGGAC(10/14) BsoBI 298 CCCGAG C^[CT]CG[GA]G BspEI 635 TCCGGA T^CCGGA BsrI 723 CCAGT ACTGG(1/-1) BssHII 244 GCGCGC G^CGCGC BstAPI 141 GCACGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 240 GGATG GGATG(2/0) BstXI 230 CCAGCTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 387 AGATCC [GA]^GATC[CT] DraII 413 AGGGCCC [GA]G^G[ACGT]CC[CT] DraIII 142 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 459 GACCAAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 198 CTCTTC CTCTTC(1/4) Eco47III 327 AGCGCT AGC^GCT EcoNI 632 CCTTCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 413 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoRI 520 GAATTC G^AATTC EcoT14I 368 CCTTGG C^C[AT][AT]GG FokI 240 GGATG GGATG(9/13) KasI 21 GGCGCC G^GCGCC Ksp632I 198 CTCTTC CTCTTC(1/4) MflI 387 AGATCC [GA]^GATC[CT] MroI 635 TCCGGA T^CCGGA NaeI 18 GCCGGC GCC^GGC NarI 21 GGCGCC GG^CGCC NgoMIV 18 GCCGGC G^CCGGC PleI 732 GACTC GAGTC(4/5) PvuII 253 CAGCTG CAG^CTG SacI 772 GAGCTC GAGCT^C SapI 197 GCTCTTC GCTCTTC(1/4) SbfI 427 CCTGCAGG CCTGCA^GG SfoI 21 GGCGCC GGC^GCC SgrAI 23 CGCCGGTG C[GA]^CCGG[CT]G SmlI 331 CTCAAG C^T[CT][GA]AG Sse8387I 427 CCTGCAGG CCTGCA^GG StyI 368 CCTTGG C^C[AT][AT]GG TaqI 452 TCGA T^CGA TfiI 716 GATTC G^A[AT]TC Tsp509I 521 AATT ^AATT TthHB8I 452 TCGA T^CGA XcmI 359 CCAACCTGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 387 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 21 GGCGCC G[GA]^CG[CT]C 494 GACGTC AspHI 148 GTGCAC G[AT]GC[AT]^C 772 GAGCTC BanII 414 GGGCCC G[GA]GC[CT]^C 772 GAGCTC BcnI 177 CCCGG CC^[GC]GG 217 CCGGG BpmI 470 CTCCAG CTGGAG(16/14) 311 CTGGAG BsaHI 21 GGCGCC G[GA]^CG[CT]C 494 GACGTC BsiHKAI 148 GTGCAC G[AT]GC[AT]^C 772 GAGCTC BsmAI 656 GAGAC GTCTC(1/5) 468 GTCTC BsrBI 580 GAGCGG CCGCTC(-3/-3) 598 GAGCGG BsrFI 18 GCCGGC [GA]^CCGG[CT] 24 GCCGGT Cfr10I 18 GCCGGC [GA]^CCGG[CT] 24 GCCGGT FauI 259 CCCGC CCCGC(4/6) 407 GCGGG HaeII 21 GGCGCC [GA]GCGC^[CT] 327 AGCGCT Hin1I 21 GGCGCC G[GA]^CG[CT]C 494 GACGTC HinfI 716 GATTC G^A[ACGT]TC 732 GACTC MaeII 455 ACGT A^CGT 495 ACGT MaeIII 457 GTGAC ^GT[ACGT]AC 614 GTGAC MboII 385 GAAGA GAAGA(8/7) 199 TCTTC MspA1I 253 CAGCTG C[AC]G^C[GT]G 308 CCGCTG NciI 177 CCCGG CC^[GC]GG 217 CCGGG PstI 46 CTGCAG CTGCA^G 428 CTGCAG SfaNI 241 GATGC GCATC(5/9) 156 GCATC SfcI 46 CTGCAG C^T[GA][CT]AG 428 CTGCAG Tsp45I 457 GTGAC ^GT[GC]AC 614 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AccII AciI AluI Asp700I BanI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BspMI BssKI BstNI BstUI Cac8I CfoI Cfr13I DpnII EaeI EcoRII Fnu4HI HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MboI MnlI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI TspRI XmnI