Restriction map for
pFN21AB8350
(Circular) (Six-base) MAPSORT of: pFN21AB8350.seq Check: 6817 from: 1 to: 5503
pFN21AB8350 5503 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2166 2426 2664 2166
Size: 260 238 5005
Fragments arranged by size:
5005 260 238
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2503 3313 278
Size: 53 83 186 936 967 810 2468
Fragments arranged by size:
2468 967 936 810 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2846 1937
Size: 909 4594
Acc65I G'GTAC_C
Cuts at: 2824 2824
Size: 5503
AclI AA'CG_TT
Cuts at: 1563 3630 4003 1563
Size: 2067 373 3063
Fragments arranged by size:
3063 2067 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2060 3566 4950 5218 1632
Size: 428 1506 1384 268 1917
Fragments arranged by size:
1917 1506 1384 428 268
AfeI AGC'GCT
Cuts at: 2334 2334
Size: 5503
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5306
AflIII A'CryG_T
Cuts at: 1203 4402 5379 1203
Size: 3199 977 1327
Fragments arranged by size:
3199 1327 977
AgeI A'CCGG_T
Cuts at: 4334 4334
Size: 5503
AhdI GACnn_n'nnGTC
Cuts at: 4232 4232
Size: 5503
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2036 1184
Size: 852 4651
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2310 2342 2310
Size: 32 5471
AlwNI CAG_nnn'CTG
Cuts at: 3246 4818 3246
Size: 1572 3931
ApaI G_GGCC'C
Cuts at: 2423 2423
Size: 5503
ApaLI G'TGCA_C
Cuts at: 2153 3560 4716 2153
Size: 1407 1156 2940
Fragments arranged by size:
2940 1407 1156
ApoI r'AATT_y
Cuts at: 1493 2525 2812 3038 3190 5299 1493
Size: 1032 287 226 152 2109 1697
Fragments arranged by size:
2109 1697 1032 287 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4057 160
Size: 872 3025 1606
Fragments arranged by size:
3025 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
AvaI C'yCGr_G
Cuts at: 1870 1943 2303 2828 1870
Size: 73 360 525 4545
Fragments arranged by size:
4545 525 360 73
BamHI G'GATC_C
Cuts at: 2833 3200 2833
Size: 367 5136
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2032 2447 2824 4279
Size: 325 217 627 239 6 415 377 1455
Cuts at: 4279 618
Size: 1842
Fragments arranged by size:
1842 1455 627 415 377 325 239 217
6
BanII G_rGCy'C
Cuts at: 1391 2423 2781 2822 1391
Size: 1032 358 41 4072
Fragments arranged by size:
4072 1032 358 41
BbeI G_GCGC'C
Cuts at: 2030 2030
Size: 5503
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4532
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3694 3728 1903
Size: 34 1757 34 3678
Fragments arranged by size:
3678 1757 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3395 4605 3395
Size: 1210 4293
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5503
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2369 4114 136
Size: 107 122 71 631 605 697 1745 1525
Fragments arranged by size:
1745 1525 697 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5498 5498
Size: 5503
BlpI GC'TnA_GC
Cuts at: 2920 2920
Size: 5503
Bme1580I G_kGCm'C
Cuts at: 1790 2157 2228 2423 2452 3564 4720 1790
Size: 367 71 195 29 1112 1156 2573
Fragments arranged by size:
2573 1156 1112 367 195 71 29
BmgBI CAC'GTC
Cuts at: 1318 2461 1318
Size: 1143 4360
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3234 4192 442
Size: 416 53 83 399 24 1817 958 1753
Fragments arranged by size:
1817 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2337 2460 4163 1239
Size: 1098 123 1703 2579
Fragments arranged by size:
2579 1703 1098 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2321 3628 4493 4791 2321
Size: 1307 865 298 3033
Fragments arranged by size:
3033 1307 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2655 4166 882
Size: 617 1156 1511 2219
Fragments arranged by size:
2219 1511 1156 617
BsaAI yAC'GTr
Cuts at: 493 1204 3294 493
Size: 711 2090 2702
Fragments arranged by size:
2702 2090 711
BsaBI GATnn'nnATC
Cuts at: 5497 5497
Size: 5503
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 2500 3310 3692
Size: 53 83 186 936 494 473 810 382
Cuts at: 3692 4327 275
Size: 635 1451
Fragments arranged by size:
1451 936 810 635 494 473 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2640 3936 4334 4608 4755 5166 2640
Size: 1296 398 274 147 411 2977
Fragments arranged by size:
2977 1296 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2310 2340 1178
Size: 30 165 30 907 30 4341
Fragments arranged by size:
4341 907 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 1186
Size: 1258 4245
BseYI C'CCAG_C
Cuts at: 2173 2443 4706 5202 2173
Size: 270 2263 496 2474
Fragments arranged by size:
2474 2263 496 270
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4930
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2410 2953 3714 4742 1540
Size: 275 186 409 543 761 1028 2301
Fragments arranged by size:
2301 1028 761 543 409 275 186
BsiHKAI G_wGCw'C
Cuts at: 2157 2781 3564 3649 4720 2157
Size: 624 783 85 1071 2940
Fragments arranged by size:
2940 1071 783 624 85
BsmI GAATG_Cn'
Cuts at: 3014 3107 3014
Size: 93 5410
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2228 2423 2452 2781 2822 3564
Size: 399 367 71 195 29 329 41 742
Cuts at: 3564 3649 4720 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 367 329 195 85
71 41 29
BspEI T'CCGG_A
Cuts at: 2640 5166 2640
Size: 2526 2977
BspHI T'CATG_A
Cuts at: 3392 5122 3392
Size: 1730 3773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2166 2426 2664 2858 844
Size: 1322 260 238 194 3489
Fragments arranged by size:
3489 1322 260 238 194
BsrBI CCG'CTC
Cuts at: 1394 1823 2587 2605 3390 1394
Size: 429 764 18 785 3507
Fragments arranged by size:
3507 785 764 429 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 3998 4172 65
Size: 1183 2750 174 1396
Fragments arranged by size:
2750 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2023 2029 4147 4334 1700
Size: 110 213 6 2118 187 2869
Fragments arranged by size:
2869 2118 213 187 110 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5503
BssHII G'CGCG_C
Cuts at: 1925 2249 1925
Size: 324 5179
BssSI C'ACGA_G
Cuts at: 3563 4575 3563
Size: 1012 4491
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2152 794
Size: 1358 4145
BstBI TT'CG_AA
Cuts at: 4307 4307
Size: 5503
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5503
BstXI CCAn_nnnn'nTGG
Cuts at: 2242 2242
Size: 5503
BstYI r'GATC_y
Cuts at: 1972 2392 2833 3200 3585 3602 5054 5140 5152
Size: 420 441 367 385 17 1452 86 12
Cuts at: 5152 5498 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 441 420 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4012
Fragments arranged by size:
4012 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4764
Fragments arranged by size:
4764 683 56
BtsI GCAGTG_nn'
Cuts at: 2088 3019 3813 3833 2088
Size: 931 794 20 3758
Fragments arranged by size:
3758 931 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2524 2559 529
Size: 35 1960 35 3473
Fragments arranged by size:
3473 1960 35 35
DraI TTT'AAA
Cuts at: 2807 2807
Size: 5503
DraIII CAC_nnn'GTG
Cuts at: 2152 2152
Size: 5503
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2470 4510 809
Size: 507 1154 2040 1802
Fragments arranged by size:
2040 1802 1154 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2184 2397 2545
Size: 54 1425 50 275 18 354 213 148
Cuts at: 2545 2950 3839 5468 8
Size: 405 889 1629 43
Fragments arranged by size:
1629 1425 889 405 354 275 213 148
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2950 1537
Size: 275 1138 4090
Fragments arranged by size:
4090 1138 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2209 3433 1353
Size: 856 1224 3423
Fragments arranged by size:
3423 1224 856
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4090 4464 4610 387
Size: 1064 138 2501 374 146 1280
Fragments arranged by size:
2501 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2779 2779
Size: 5503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2337 2460 3566 4163 4950 5218
Size: 393 428 277 123 1106 597 787 268
Cuts at: 5218 1239
Size: 1524
Fragments arranged by size:
1524 1106 787 597 428 393 277 268
123
EcoNI CCTnn'n_nnAGG
Cuts at: 2641 2641
Size: 5503
EcoO109I rG'GnC_Cy
Cuts at: 2419 2419
Size: 5503
EcoRI G'AATT_C
Cuts at: 2525 2812 3190 2525
Size: 287 378 4838
Fragments arranged by size:
4838 378 287
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 5503
FspI TGC'GCA
Cuts at: 4009 4009
Size: 5503
HaeII r_GCGC'y
Cuts at: 1678 2030 2336 4650 1678
Size: 352 306 2314 2531
Fragments arranged by size:
2531 2314 352 306
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4144 4176 4218 4250 1373
Size: 32 135 32 2572 32 42 32 2626
Fragments arranged by size:
2626 2572 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2847 3093 669
Size: 988 281 909 246 3079
Fragments arranged by size:
3079 988 909 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5503
HpaI GTT'AAC
Cuts at: 3093 3093
Size: 5503
KasI G'GCGC_C
Cuts at: 2026 2026
Size: 5503
KpnI G_GTAC'C
Cuts at: 2828 2828
Size: 5503
MfeI C'AATT_G
Cuts at: 3102 3102
Size: 5503
MluI A'CGCG_T
Cuts at: 5379 5379
Size: 5503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4617 4801 1305
Size: 3312 184 2007
Fragments arranged by size:
3312 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3681
Fragments arranged by size:
3681 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3461 3820 3979 518
Size: 648 2295 359 159 2042
Fragments arranged by size:
2295 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2260 2315 2919 3596 4744 4989 1606
Size: 654 55 604 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 654 604 245 55
NaeI GCC'GGC
Cuts at: 2025 2025
Size: 5503
NarI GG'CG_CC
Cuts at: 2027 2027
Size: 5503
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4012
Fragments arranged by size:
4012 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5503
NgoMIV G'CCGG_C
Cuts at: 2023 2023
Size: 5503
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5503
NotI GC'GGCC_GC
Cuts at: 2950 2950
Size: 5503
NspI r_CATG'y
Cuts at: 1132 1229 2861 4406 1132
Size: 97 1632 1545 2229
Fragments arranged by size:
2229 1632 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5503
PciI A'CATG_T
Cuts at: 4402 4402
Size: 5503
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5503
PfoI T'CCnGG_A
Cuts at: 2732 2732
Size: 5503
PmeI GTTT'AAAC
Cuts at: 2807 2807
Size: 5503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2310 2342 3572 3604 5085 5117 2310
Size: 32 1230 32 1481 32 2696
Fragments arranged by size:
2696 1481 1230 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5503
PsiI TTA'TAA
Cuts at: 3073 3073
Size: 5503
PspOMI G'GGCC_C
Cuts at: 2419 2419
Size: 5503
PstI C_TGCA'G
Cuts at: 830 2055 2437 2855 830
Size: 1225 382 418 3478
Fragments arranged by size:
3478 1225 418 382
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5503
PvuII CAG'CTG
Cuts at: 2260 2260
Size: 5503
SacI G_AGCT'C
Cuts at: 2781 2781
Size: 5503
SalI G'TCGA_C
Cuts at: 1936 2845 1936
Size: 909 4594
SapI GCTCTTCn'nnn_
Cuts at: 2209 2209
Size: 5503
SbfI CC_TGCA'GG
Cuts at: 2437 2855 2437
Size: 418 5085
ScaI AGT'ACT
Cuts at: 3751 5463 3751
Size: 1712 3791
SfcI C'TryA_G
Cuts at: 826 1045 2051 2433 2851 3986 4667 4858 826
Size: 219 1006 382 418 1135 681 191 1471
Fragments arranged by size:
1471 1135 1006 681 418 382 219 191
SfoI GGC'GCC
Cuts at: 2028 2028
Size: 5503
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
SgrAI Cr'CCGG_yG
Cuts at: 1700 2029 1700
Size: 329 5174
SmaI CCC'GGG
Cuts at: 1872 2830 1872
Size: 958 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2336 3607 4508 4770 820
Size: 197 926 393 1271 901 262 1553
Fragments arranged by size:
1553 1271 926 901 393 262 197
SnaBI TAC'GTA
Cuts at: 493 3294 493
Size: 2801 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5503
SphI G_CATG'C
Cuts at: 1132 2861 1132
Size: 1729 3774
SspI AAT'ATT
Cuts at: 5 52 3427 5
Size: 47 3375 2081
Fragments arranged by size:
3375 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5503
StyI C'CwwG_G
Cuts at: 513 1065 2004 2373 2942 513
Size: 552 939 369 569 3074
Fragments arranged by size:
3074 939 569 552 369
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2396 2435 3530 3547 3700 3885 4330
Size: 1255 816 39 1095 17 153 185 445
Cuts at: 4330 325
Size: 1498
Fragments arranged by size:
1498 1255 1095 816 445 185 153 39
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3749 5461 96
Size: 274 80 33 51 1443 1772 1712 138
Fragments arranged by size:
1772 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5463 5495 5463
Size: 32 5471
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5503
XbaI T'CTAG_A
Cuts at: 2839 2839
Size: 5503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2371 2371
Size: 5503
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5503
XmaI C'CCGG_G
Cuts at: 1870 2828 1870
Size: 958 4545
XmnI GAAnn'nnTTC
Cuts at: 2525 2693 2720 3632 2525
Size: 168 27 912 4396
Fragments arranged by size:
4396 912 168 27
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2501 3311 276
Size: 53 83 186 936 967 810 2468
Fragments arranged by size:
2468 967 936 810 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SrfI SwaI