Restriction map for
pFN21AE1864
(Circular) (Six-base) MAPSORT of: pFN21AE1864.seq Check: 4471 from: 1 to: 5860
pFN21AE1864 5860 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2855 2855
Size: 5860
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3670 278
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3203 1937
Size: 1266 4594
Acc65I G'GTAC_C
Cuts at: 3181 3181
Size: 5860
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2897 3923 5307 5575 1632
Size: 1265 1026 1384 268 1917
Fragments arranged by size:
1917 1384 1265 1026 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 4759 5736 1203
Size: 3556 977 1327
Fragments arranged by size:
3556 1327 977
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 2721 4589 2721
Size: 1868 3992
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5860
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2825 2857 2825
Size: 32 5828
AlwNI CAG_nnn'CTG
Cuts at: 3055 3118 3603 5175 3055
Size: 63 485 1572 3740
Fragments arranged by size:
3740 1572 485 63
ApaI G_GGCC'C
Cuts at: 2018 2018
Size: 5860
ApaLI G'TGCA_C
Cuts at: 3917 5073 3917
Size: 1156 4704
ApoI r'AATT_y
Cuts at: 1493 2503 3169 3395 3547 5656 1493
Size: 1010 666 226 152 2109 1697
Fragments arranged by size:
2109 1697 1010 666 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 3185 1870
Size: 73 1242 4545
Fragments arranged by size:
4545 1242 73
BamHI G'GATC_C
Cuts at: 3190 3557 3190
Size: 367 5493
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3155 3181 4636 618
Size: 325 217 627 1368 26 1455 1842
Fragments arranged by size:
1842 1455 1368 627 325 217 26
BanII G_rGCy'C
Cuts at: 1391 2018 2079 2393 2421 2479 3039 3179 1391
Size: 627 61 314 28 58 560 140 4072
Fragments arranged by size:
4072 627 560 314 140 61 58 28
BbeI G_GCGC'C
Cuts at: 3159 3159
Size: 5860
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2014 2078 2379 2534 928
Size: 971 115 64 301 155 4254
Fragments arranged by size:
4254 971 301 155 115 64
BbvCI CC'TCA_GC
Cuts at: 2145 2145
Size: 5860
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4051 4085 1903
Size: 34 2114 34 3678
Fragments arranged by size:
3678 2114 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3752 4962 3752
Size: 1210 4650
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5860
BfrBI ATG'CAT
Cuts at: 2554 2554
Size: 5860
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4471 136
Size: 107 122 71 631 605 2799 1525
Fragments arranged by size:
2799 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5855 5855
Size: 5860
BlpI GC'TnA_GC
Cuts at: 2073 3277 2073
Size: 1204 4656
Bme1580I G_kGCm'C
Cuts at: 1790 2018 2463 2622 2892 3921 5077 1790
Size: 228 445 159 270 1029 1156 2573
Fragments arranged by size:
2573 1156 1029 445 270 228 159
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2458 3062 3591
Size: 416 53 83 399 24 1041 604 529
Cuts at: 3591 4549 442
Size: 958 1753
Fragments arranged by size:
1753 1041 958 604 529 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 2455 4520 1239
Size: 1088 128 2065 2579
Fragments arranged by size:
2579 2065 1088 128
Bpu10I CC'TnA_GC
Cuts at: 2145 2145
Size: 5860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3985 4850 5148 3985
Size: 865 298 4697
Fragments arranged by size:
4697 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2252 4523 882
Size: 617 753 2271 2219
Fragments arranged by size:
2271 2219 753 617
BsaAI yAC'GTr
Cuts at: 493 1204 3651 493
Size: 711 2447 2702
Fragments arranged by size:
2702 2447 711
BsaBI GATnn'nnATC
Cuts at: 5854 5854
Size: 5860
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2722 3156 3667 4049
Size: 53 83 186 936 1189 434 511 382
Cuts at: 4049 4684 275
Size: 635 1451
Fragments arranged by size:
1451 1189 936 635 511 434 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4293 4691 4965 5112 5523 4293
Size: 398 274 147 411 4630
Fragments arranged by size:
4630 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2825 2855 1178
Size: 30 165 30 1422 30 4183
Fragments arranged by size:
4183 1422 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2623 2846 1186
Size: 1437 223 4200
Fragments arranged by size:
4200 1437 223
BseYI C'CCAG_C
Cuts at: 2011 2394 2754 5063 5559 2011
Size: 383 360 2309 496 2312
Fragments arranged by size:
2312 2309 496 383 360
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2147 1163
Size: 573 411 4876
Fragments arranged by size:
4876 573 411
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3310 4071 5099 1540
Size: 275 186 1309 761 1028 2301
Fragments arranged by size:
2301 1309 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2393 2421 2479 3039 3921 4006 5077 2393
Size: 28 58 560 882 85 1071 3176
Fragments arranged by size:
3176 1071 882 560 85 58 28
BsmI GAATG_Cn'
Cuts at: 2335 3371 3464 2335
Size: 1036 93 4731
Fragments arranged by size:
4731 1036 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2079 2393 2421 2463 2479 2622
Size: 399 228 61 314 28 42 16 143
Cuts at: 2622 2892 3039 3179 3921 4006 5077 1391
Size: 270 147 140 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 314 270 228 147
143 140 85 61 42 28 16
BspEI T'CCGG_A
Cuts at: 5523 5523
Size: 5860
BspHI T'CATG_A
Cuts at: 3749 5479 3749
Size: 1730 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2855 3040 3215 844
Size: 2011 185 175 3489
Fragments arranged by size:
3489 2011 185 175
BsrBI CCG'CTC
Cuts at: 1394 1823 3747 1394
Size: 429 1924 3507
Fragments arranged by size:
3507 1924 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4355 4529 65
Size: 1183 3107 174 1396
Fragments arranged by size:
3107 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4504 4691 1700
Size: 110 2694 187 2869
Fragments arranged by size:
2869 2694 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5860
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5860
BssSI C'ACGA_G
Cuts at: 3038 3920 4932 3038
Size: 882 1012 3966
Fragments arranged by size:
3966 1012 882
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5860
BstBI TT'CG_AA
Cuts at: 4664 4664
Size: 5860
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5860
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5860
BstYI r'GATC_y
Cuts at: 1972 2120 2364 3190 3557 3942 3959 5411 5497
Size: 148 244 826 367 385 17 1452 86
Cuts at: 5497 5509 5855 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 826 385 367 346 244 148
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2291 2606 2291
Size: 315 5545
BtgI C'CryG_G
Cuts at: 513 1065 2004 2625 513
Size: 552 939 621 3748
Fragments arranged by size:
3748 939 621 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5121
Fragments arranged by size:
5121 683 56
BtsI GCAGTG_nn'
Cuts at: 2765 3376 4170 4190 2765
Size: 611 794 20 4435
Fragments arranged by size:
4435 794 611 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5825
DraI TTT'AAA
Cuts at: 3164 3164
Size: 5860
DraIII CAC_nnn'GTG
Cuts at: 2887 2887
Size: 5860
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4867 809
Size: 507 3551 1802
Fragments arranged by size:
3551 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2487 2808 3307
Size: 54 1425 50 275 18 657 321 499
Cuts at: 3307 4196 5825 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 657 499 321 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3307 1537
Size: 275 1495 4090
Fragments arranged by size:
4090 1495 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2426 2608 3790 1353
Size: 1073 182 1182 3423
Fragments arranged by size:
3423 1182 1073 182
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4447 4821 4967 387
Size: 1064 138 2858 374 146 1280
Fragments arranged by size:
2858 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2391 2419 2477 3037 2391
Size: 28 58 560 5214
Fragments arranged by size:
5214 560 58 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2455 2897 3923 4520 5307 5575
Size: 393 695 128 442 1026 597 787 268
Cuts at: 5575 1239
Size: 1524
Fragments arranged by size:
1524 1026 787 695 597 442 393 268
128
EcoO109I rG'GnC_Cy
Cuts at: 2287 3052 2287
Size: 765 5095
EcoRI G'AATT_C
Cuts at: 3169 3547 3169
Size: 378 5482
EcoRV GAT'ATC
Cuts at: 2585 2585
Size: 5860
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 3159 5007 1678
Size: 1481 1848 2531
Fragments arranged by size:
2531 1848 1481
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2290 2322 2527 2559 2707
Size: 32 135 32 718 32 205 32 148
Cuts at: 2707 2720 2739 2752 3112 3144 4501 4533 4575
Size: 13 19 13 360 32 1357 32 42
Cuts at: 4575 4607 1373
Size: 32 2626
Fragments arranged by size:
2626 1357 718 360 205 148 135 42
32 32 32 32 32 32 32 19
13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3204 3450 669
Size: 988 281 1266 246 3079
Fragments arranged by size:
3079 1266 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5860
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KasI G'GCGC_C
Cuts at: 3155 3155
Size: 5860
KpnI G_GTAC'C
Cuts at: 3185 3185
Size: 5860
MfeI C'AATT_G
Cuts at: 3459 3459
Size: 5860
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4974 5158 1305
Size: 3669 184 2007
Fragments arranged by size:
3669 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2489 2810 10
Size: 54 1425 343 657 321 3060
Fragments arranged by size:
3060 1425 657 343 321 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2157 2669 3090 3818 4177 4336 518
Size: 648 991 512 421 728 359 159 2042
Fragments arranged by size:
2042 991 728 648 512 421 359 159
MspA1I CmG'CkG
Cuts at: 1606 2865 3020 3276 3953 5101 5346 1606
Size: 1259 155 256 677 1148 245 2120
Fragments arranged by size:
2120 1259 1148 677 256 245 155
NarI GG'CG_CC
Cuts at: 3156 3156
Size: 5860
NcoI C'CATG_G
Cuts at: 513 1065 2004 2625 513
Size: 552 939 621 3748
Fragments arranged by size:
3748 939 621 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5860
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5860
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NsiI A_TGCA'T
Cuts at: 2556 2556
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 2558 2679 3218 4763 1132
Size: 97 1329 121 539 1545 2229
Fragments arranged by size:
2229 1545 1329 539 121 97
PasI CC'CwG_GG
Cuts at: 1348 2209 1348
Size: 861 4999
PciI A'CATG_T
Cuts at: 4759 4759
Size: 5860
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2437 1108
Size: 1329 4531
PfoI T'CCnGG_A
Cuts at: 2059 2343 3054 2059
Size: 284 711 4865
Fragments arranged by size:
4865 711 284
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2857 3929 3961 5442 5474 2825
Size: 32 1072 32 1481 32 3211
Fragments arranged by size:
3211 1481 1072 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2287 3052 2287
Size: 765 5095
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5860
PsiI TTA'TAA
Cuts at: 2094 3430 2094
Size: 1336 4524
PspOMI G'GGCC_C
Cuts at: 2014 2014
Size: 5860
PstI C_TGCA'G
Cuts at: 830 2571 3025 3051 3212 830
Size: 1741 454 26 161 3478
Fragments arranged by size:
3478 1741 454 161 26
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
PvuII CAG'CTG
Cuts at: 3020 3020
Size: 5860
SacI G_AGCT'C
Cuts at: 2393 2421 2479 3039 2393
Size: 28 58 560 5214
Fragments arranged by size:
5214 560 58 28
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SapI GCTCTTCn'nnn_
Cuts at: 2426 2426
Size: 5860
SbfI CC_TGCA'GG
Cuts at: 2571 3051 3212 2571
Size: 480 161 5219
Fragments arranged by size:
5219 480 161
ScaI AGT'ACT
Cuts at: 4108 5820 4108
Size: 1712 4148
SfcI C'TryA_G
Cuts at: 826 1045 2567 3021 3047 3208 4343 5024 5215
Size: 219 1522 454 26 161 1135 681 191
Cuts at: 5215 826
Size: 1471
Fragments arranged by size:
1522 1471 1135 681 454 219 191 161
26
SfoI GGC'GCC
Cuts at: 3157 3157
Size: 5860
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 3187 1872
Size: 1315 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3964 4865 5127 820
Size: 197 926 2021 901 262 1553
Fragments arranged by size:
2021 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5860
SphI G_CATG'C
Cuts at: 1132 2558 3218 1132
Size: 1426 660 3774
Fragments arranged by size:
3774 1426 660
SspI AAT'ATT
Cuts at: 5 52 3784 5
Size: 47 3732 2081
Fragments arranged by size:
3732 2081 47
StuI AGG'CCT
Cuts at: 1517 2651 1517
Size: 1134 4726
StyI C'CwwG_G
Cuts at: 513 1065 2004 2484 2625 2824 3299 513
Size: 552 939 480 141 199 475 3074
Fragments arranged by size:
3074 939 552 480 475 199 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2768 2794 3081 3887 3904 4057 4242
Size: 1255 1188 26 287 806 17 153 185
Cuts at: 4242 4687 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1188 806 445 287 185 153
26 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4106 5818 96
Size: 274 80 33 51 1443 2129 1712 138
Fragments arranged by size:
2129 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5820 5852 5820
Size: 32 5828
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5860
XbaI T'CTAG_A
Cuts at: 2587 3196 2587
Size: 609 5251
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 3185 1870
Size: 1315 4545
XmnI GAAnn'nnTTC
Cuts at: 3989 3989
Size: 5860
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3668 276
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I
EcoNI FalI FseI FspAI NaeI NgoMIV NruI PacI
PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SrfI SwaI XcmI