(Circular) (Six-base) MAPSORT of: pFN21AE0302.seq Check: 4893 from: 1 to: 6133 pFN21AE0302 6133 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3943 278 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3476 1937 Size: 1539 4594 Acc65I G'GTAC_C Cuts at: 2934 3454 2934 Size: 520 5613 AclI AA'CG_TT Cuts at: 1563 4260 4633 1563 Size: 2697 373 3063 Fragments arranged by size: 3063 2697 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4196 5580 5848 1632 Size: 2564 1384 268 1917 Fragments arranged by size: 2564 1917 1384 268 AfeI AGC'GCT Cuts at: 2123 2123 Size: 6133 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5936 AflIII A'CryG_T Cuts at: 1203 2425 5032 6009 1203 Size: 1222 2607 977 1327 Fragments arranged by size: 2607 1327 1222 977 AgeI A'CCGG_T Cuts at: 4964 4964 Size: 6133 AhdI GACnn_n'nnGTC Cuts at: 4862 4862 Size: 6133 AleI CACnn'nnGTG Cuts at: 1166 2057 1166 Size: 891 5242 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6133 AlwNI CAG_nnn'CTG Cuts at: 2388 3876 5448 2388 Size: 1488 1572 3073 Fragments arranged by size: 3073 1572 1488 ApaLI G'TGCA_C Cuts at: 2279 4190 5346 2279 Size: 1911 1156 3066 Fragments arranged by size: 3066 1911 1156 ApoI r'AATT_y Cuts at: 1493 3442 3668 3820 5929 1493 Size: 1949 226 152 2109 1697 Fragments arranged by size: 2109 1949 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4687 160 Size: 872 3655 1606 Fragments arranged by size: 3655 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 AvaI C'yCGr_G Cuts at: 1870 1943 2107 3096 3104 3458 1870 Size: 73 164 989 8 354 4545 Fragments arranged by size: 4545 989 354 164 73 8 BamHI G'GATC_C Cuts at: 2730 2854 3463 3830 2730 Size: 124 609 367 5033 Fragments arranged by size: 5033 609 367 124 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2934 3454 4909 618 Size: 325 217 627 1147 520 1455 1842 Fragments arranged by size: 1842 1455 1147 627 520 325 217 BanII G_rGCy'C Cuts at: 1391 2066 2078 2114 2653 3218 3296 3452 1391 Size: 675 12 36 539 565 78 156 4072 Fragments arranged by size: 4072 675 565 539 156 78 36 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2037 2428 2777 928 Size: 971 138 391 349 4284 Fragments arranged by size: 4284 971 391 349 138 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2003 2037 2878 2912 4324 4358 1903 Size: 34 66 34 841 34 1412 34 3678 Fragments arranged by size: 3678 1412 841 66 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2303 4025 5235 2303 Size: 1722 1210 3201 Fragments arranged by size: 3201 1722 1210 BclI T'GATC_A Cuts at: 1548 2259 3321 1548 Size: 711 1062 4360 Fragments arranged by size: 4360 1062 711 BfrBI ATG'CAT Cuts at: 3165 3165 Size: 6133 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4744 136 Size: 107 122 71 631 605 3072 1525 Fragments arranged by size: 3072 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6128 6128 Size: 6133 BlpI GC'TnA_GC Cuts at: 3550 3550 Size: 6133 Bme1580I G_kGCm'C Cuts at: 1790 2283 4194 5350 1790 Size: 493 1911 1156 2573 Fragments arranged by size: 2573 1911 1156 493 BmgBI CAC'GTC Cuts at: 1318 2059 2426 1318 Size: 741 367 5025 Fragments arranged by size: 5025 741 367 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2425 3864 4822 Size: 416 53 83 399 24 1008 1439 958 Cuts at: 4822 442 Size: 1753 Fragments arranged by size: 1753 1439 1008 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3035 3281 4793 1239 Size: 1796 246 1512 2579 Fragments arranged by size: 2579 1796 1512 246 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4258 5123 5421 4258 Size: 865 298 4970 Fragments arranged by size: 4970 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4796 882 Size: 617 3297 2219 Fragments arranged by size: 3297 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3924 493 Size: 711 2720 2702 Fragments arranged by size: 2720 2702 711 BsaBI GATnn'nnATC Cuts at: 3077 3326 6127 3077 Size: 249 2801 3083 Fragments arranged by size: 3083 2801 249 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2570 3315 3940 4322 Size: 53 83 186 936 1037 745 625 382 Cuts at: 4322 4957 275 Size: 635 1451 Fragments arranged by size: 1451 1037 936 745 635 625 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2400 4566 4964 5238 5385 5796 2400 Size: 2166 398 274 147 411 2737 Fragments arranged by size: 2737 2166 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2033 2063 2084 2087 2093 Size: 30 165 30 630 30 21 3 6 Cuts at: 2093 2114 2117 2585 2615 1178 Size: 21 3 468 30 4696 Fragments arranged by size: 4696 630 468 165 30 30 30 30 21 21 6 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2084 2092 1186 Size: 898 8 5227 Fragments arranged by size: 5227 898 8 BseYI C'CCAG_C Cuts at: 2454 2458 2741 5336 5832 2454 Size: 4 283 2595 496 2755 Fragments arranged by size: 2755 2595 496 283 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5560 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3583 4344 5372 1540 Size: 275 186 1582 761 1028 2301 Fragments arranged by size: 2301 1582 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2283 2311 3111 4194 4279 5350 2283 Size: 28 800 1083 85 1071 3066 Fragments arranged by size: 3066 1083 1071 800 85 28 BsmI GAATG_Cn' Cuts at: 3644 3737 3644 Size: 93 6040 BsmBI CGTCTCn'nnnn_ Cuts at: 2549 2549 Size: 6133 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2078 2114 2283 2311 2653 3111 Size: 399 276 12 36 169 28 342 458 Cuts at: 3111 3218 3296 3452 4194 4279 5350 1391 Size: 107 78 156 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 458 399 342 276 169 156 107 85 78 36 28 12 BspEI T'CCGG_A Cuts at: 2400 5796 2400 Size: 3396 2737 BspHI T'CATG_A Cuts at: 2703 4022 5752 2703 Size: 1319 1730 3084 Fragments arranged by size: 3084 1730 1319 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2946 2988 3488 844 Size: 2102 42 500 3489 Fragments arranged by size: 3489 2102 500 42 BsrBI CCG'CTC Cuts at: 1394 1823 3256 4020 1394 Size: 429 1433 764 3507 Fragments arranged by size: 3507 1433 764 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3400 4628 4802 65 Size: 1183 2152 1228 174 1396 Fragments arranged by size: 2152 1396 1228 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4777 4964 1700 Size: 110 2967 187 2869 Fragments arranged by size: 2967 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6133 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6133 BssSI C'ACGA_G Cuts at: 4193 5205 4193 Size: 1012 5121 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6133 BstBI TT'CG_AA Cuts at: 4937 4937 Size: 6133 BstEII G'GTnAC_C Cuts at: 1184 3265 1184 Size: 2081 4052 BstXI CCAn_nnnn'nTGG Cuts at: 2126 2126 Size: 6133 BstYI r'GATC_y Cuts at: 1972 2730 2854 3030 3463 3830 4215 4232 5684 Size: 758 124 176 433 367 385 17 1452 Cuts at: 5684 5770 5782 6128 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 758 433 385 367 346 176 124 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2067 2067 Size: 6133 BtgI C'CryG_G Cuts at: 513 1065 2052 2577 513 Size: 552 987 525 4069 Fragments arranged by size: 4069 987 552 525 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5394 Fragments arranged by size: 5394 683 56 BtsI GCAGTG_nn' Cuts at: 2281 3649 4443 4463 2281 Size: 1368 794 20 3951 Fragments arranged by size: 3951 1368 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6133 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6098 DraI TTT'AAA Cuts at: 2503 3437 2503 Size: 934 5199 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5140 809 Size: 507 3824 1802 Fragments arranged by size: 3824 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2274 2520 2673 Size: 54 1425 50 275 18 444 246 153 Cuts at: 2673 2691 3171 3580 4469 6098 8 Size: 18 480 409 889 1629 43 Fragments arranged by size: 1629 1425 889 480 444 409 275 246 153 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3580 1537 Size: 275 1768 4090 Fragments arranged by size: 4090 1768 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2789 4063 1353 Size: 1436 1274 3423 Fragments arranged by size: 3423 1436 1274 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4720 5094 5240 387 Size: 1064 138 3131 374 146 1280 Fragments arranged by size: 3131 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3035 3281 4196 4793 5580 5848 1239 Size: 393 1403 246 915 597 787 268 1524 Fragments arranged by size: 1524 1403 915 787 597 393 268 246 EcoO109I rG'GnC_Cy Cuts at: 2243 3357 2243 Size: 1114 5019 EcoRI G'AATT_C Cuts at: 3442 3820 3442 Size: 378 5755 FspI TGC'GCA Cuts at: 3140 4639 3140 Size: 1499 4634 FspAI rTGC'GCAy Cuts at: 3140 3140 Size: 6133 HaeII r_GCGC'y Cuts at: 1678 2125 3104 3234 5280 1678 Size: 447 979 130 2046 2531 Fragments arranged by size: 2531 2046 979 447 130 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2063 2065 2095 2764 Size: 32 135 32 461 30 2 30 669 Cuts at: 2764 2796 2940 2972 4774 4806 4848 4880 1373 Size: 32 144 32 1802 32 42 32 2626 Fragments arranged by size: 2626 1802 669 461 144 135 42 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 3477 3723 669 Size: 988 281 1539 246 3079 Fragments arranged by size: 3079 1539 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6133 HpaI GTT'AAC Cuts at: 3723 3723 Size: 6133 KpnI G_GTAC'C Cuts at: 2938 3458 2938 Size: 520 5613 MfeI C'AATT_G Cuts at: 3732 3732 Size: 6133 MluI A'CGCG_T Cuts at: 6009 6009 Size: 6133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2061 5247 5431 1305 Size: 756 3186 184 2007 Fragments arranged by size: 3186 2007 756 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2675 2693 3173 10 Size: 54 1425 343 843 18 480 2970 Fragments arranged by size: 2970 1425 843 480 343 54 18 MslI CAynn'nnrTG Cuts at: 518 1166 2057 2237 2550 2684 2822 4091 4450 Size: 648 891 180 313 134 138 1269 359 Cuts at: 4450 4609 518 Size: 159 2042 Fragments arranged by size: 2042 1269 891 648 359 313 180 159 138 134 MspA1I CmG'CkG Cuts at: 1606 2458 3549 4226 5374 5619 1606 Size: 852 1091 677 1148 245 2120 Fragments arranged by size: 2120 1148 1091 852 677 245 NcoI C'CATG_G Cuts at: 513 1065 2577 513 Size: 552 1512 4069 Fragments arranged by size: 4069 1512 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6133 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6133 NotI GC'GGCC_GC Cuts at: 3580 3580 Size: 6133 NruI TCG'CGA Cuts at: 2587 2587 Size: 6133 NsiI A_TGCA'T Cuts at: 3167 3167 Size: 6133 NspI r_CATG'y Cuts at: 1132 1229 2599 3165 3491 5036 1132 Size: 97 1370 566 326 1545 2229 Fragments arranged by size: 2229 1545 1370 566 326 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6133 PciI A'CATG_T Cuts at: 5032 5032 Size: 6133 PflMI CCAn_nnn'nTGG Cuts at: 1108 2991 1108 Size: 1883 4250 PfoI T'CCnGG_A Cuts at: 3258 3258 Size: 6133 PmeI GTTT'AAAC Cuts at: 3437 3437 Size: 6133 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4202 4234 5715 5747 4202 Size: 32 1481 32 4588 Fragments arranged by size: 4588 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2243 3357 2243 Size: 1114 5019 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6133 PsiI TTA'TAA Cuts at: 3703 3703 Size: 6133 PstI C_TGCA'G Cuts at: 830 3485 830 Size: 2655 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6133 PvuII CAG'CTG Cuts at: 2458 2458 Size: 6133 SalI G'TCGA_C Cuts at: 1936 3475 1936 Size: 1539 4594 SanDI GG'GwC_CC Cuts at: 2243 2243 Size: 6133 SbfI CC_TGCA'GG Cuts at: 3485 3485 Size: 6133 ScaI AGT'ACT Cuts at: 4381 6093 4381 Size: 1712 4421 SexAI A'CCwGG_T Cuts at: 3195 3195 Size: 6133 SfcI C'TryA_G Cuts at: 826 1045 2044 3481 4616 5297 5488 826 Size: 219 999 1437 1135 681 191 1471 Fragments arranged by size: 1471 1437 1135 999 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6133 SmaI CCC'GGG Cuts at: 1872 2109 3460 1872 Size: 237 1351 4545 Fragments arranged by size: 4545 1351 237 SmlI C'TyrA_G Cuts at: 820 1017 1943 4237 5138 5400 820 Size: 197 926 2294 901 262 1553 Fragments arranged by size: 2294 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3924 493 Size: 3431 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6133 SphI G_CATG'C Cuts at: 1132 3491 1132 Size: 2359 3774 SspI AAT'ATT Cuts at: 5 52 4057 5 Size: 47 4005 2081 Fragments arranged by size: 4005 2081 47 StuI AGG'CCT Cuts at: 1517 2088 1517 Size: 571 5562 StyI C'CwwG_G Cuts at: 513 1065 2577 2754 2765 2959 3572 513 Size: 552 1512 177 11 194 613 3074 Fragments arranged by size: 3074 1512 613 552 194 177 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2468 4160 4177 4330 4515 4960 325 Size: 1255 888 1692 17 153 185 445 1498 Fragments arranged by size: 1692 1498 1255 888 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3018 4379 6091 Size: 274 80 33 51 1443 1041 1361 1712 Cuts at: 6091 96 Size: 138 Fragments arranged by size: 1712 1443 1361 1041 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6093 6125 6093 Size: 32 6101 Tth111I GACn'n_nGTC Cuts at: 1316 3197 1316 Size: 1881 4252 XbaI T'CTAG_A Cuts at: 3469 3469 Size: 6133 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6133 XmaI C'CCGG_G Cuts at: 1870 2107 3458 1870 Size: 237 1351 4545 Fragments arranged by size: 4545 1351 237 XmnI GAAnn'nnTTC Cuts at: 4262 4262 Size: 6133 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3941 276 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI