################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII ClaI CpoI DraI DraII DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoT22I FbaI FseI FspI HincII HindII HpaI KpnI Ksp632I KspI MaeII MamI MboII MluI MluNI MroI MscI MseI MslI MspA1I MvnI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp45I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 268 GTCTAC GT^[AC][GT]AC AciI 717 GCGG CCGC(-3/-1) Alw44I 488 GTGCAC G^TGCAC AlwI 339 GATCC GGATC(4/5) AlwNI 185 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 488 GTGCAC G^TGCAC AspI 96 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 287 CTCGAG C^[CT]CG[GA]G AvaII 646 GGACC G^G[AT]CC BanII 401 GAGCTC G[GA]GC[CT]^C BbeI 809 GGCGCC GGCGC^C BfaI 406 CTAG C^TAG BglII 591 AGATCT A^GATCT BpmI 371 CTGGAG CTGGAG(16/14) BsmAI 676 GTCTC GTCTC(1/5) BsmI 435 GAATGC GAATGC(1/-1) BsoBI 287 CTCGAG C^[CT]CG[GA]G BspMI 547 ACCTGC ACCTGC(4/8) Cac8I 352 GCAGGC GC[ACGT]^[ACGT]GC CfoI 810 GCGC GCG^C DrdI 647 GACCTGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 651 TGGCCG [CT]^GGCC[GA] EcoRI 530 GAATTC G^AATTC EcoRV 557 GATATC GAT^ATC EcoT14I 208 CCTTGG C^C[AT][AT]GG FauI 717 GCGGG CCCGC(4/6) HaeII 809 GGCGCC [GA]GCGC^[CT] HgaI 569 GCGTC GACGC(5/10) HhaI 810 GCGC GCG^C HinP1I 810 GCGC G^CGC HindIII 387 AAGCTT A^AGCTT KasI 809 GGCGCC G^GCGCC MaeI 406 CTAG C^TAG MfeI 743 CAATTG C^AATTG MunI 743 CAATTG C^AATTG NarI 809 GGCGCC GG^CGCC NspI 57 ACATGC [GA]CATG^[CT] PaeR7I 287 CTCGAG C^TCGAG PflFI 96 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 457 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 658 CTGCAG CTGCA^G SacI 401 GAGCTC GAGCT^C SfaNI 714 GATGC GCATC(5/9) SfcI 658 CTGCAG C^T[GA][CT]AG SfoI 809 GGCGCC GGC^GCC StuI 617 AGGCCT AGG^CCT StyI 208 CCTTGG C^C[AT][AT]GG TaqI 288 TCGA T^CGA Tth111I 96 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 288 TCGA T^CGA Van91I 457 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 691 CCAAAGCCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 287 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcsI 481 AAATTC [GA]^AATT[CT] 530 GAATTC AcyI 568 GGCGTC G[GA]^CG[CT]C 809 GGCGCC AfaI 116 GTAC GT^AC 295 GTAC AocI 393 CCTGAGG CC^T[ACGT]AGG 613 CCTGAGG ApoI 481 AAATTC [GA]^AATT[CT] 530 GAATTC AspHI 401 GAGCTC G[AT]GC[AT]^C 488 GTGCAC BanI 376 GGTGCC G^G[CT][GA]CC 809 GGCGCC BsaHI 568 GGCGTC G[GA]^CG[CT]C 809 GGCGCC BseRI 535 CTCCTC GAGGAG(10/8) 253 GAGGAG BsiHKAI 401 GAGCTC G[AT]GC[AT]^C 488 GTGCAC BsrFI 307 GCCGGT [GA]^CCGG[CT] 812 GCCGGT BsrI 629 CCAGT ACTGG(1/-1) 638 CCAGT BstF5I 150 GGATG GGATG(2/0) 713 GGATG BstYI 338 AGATCC [GA]^GATC[CT] 591 AGATCT Bsu36I 393 CCTGAGG CC^T[ACGT]AGG 613 CCTGAGG Cfr10I 307 GCCGGT [GA]^CCGG[CT] 812 GCCGGT DpnII 339 GATC ^GATC 592 GATC Eco81I 393 CCTGAGG CC^T[ACGT]AGG 613 CCTGAGG FokI 150 GGATG GGATG(9/13) 713 GGATG HapII 308 CCGG C^CGG 813 CCGG Hin1I 568 GGCGTC G[GA]^CG[CT]C 809 GGCGCC HpaII 308 CCGG C^CGG 813 CCGG HphI 310 GGTGA GGTGA(8/7) 705 GGTGA MaeIII 47 GTAAC ^GT[ACGT]AC 221 GTAAC MboI 339 GATC ^GATC 592 GATC MflI 338 AGATCC [GA]^GATC[CT] 591 AGATCT MspI 308 CCGG C^CGG 813 CCGG NdeII 339 GATC ^GATC 592 GATC PleI 181 GACTC GAGTC(4/5) 266 GAGTC RsaI 116 GTAC GT^AC 295 GTAC Sau3AI 339 GATC ^GATC 592 GATC SmlI 131 CTCAAG C^T[CT][GA]AG 287 CTCGAG XhoII 338 AGATCC [GA]^GATC[CT] 591 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AluI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cfr13I DdeI EcoRII Fnu4HI HaeIII HinfI ItaI MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI TfiI TseI Tsp509I TspRI