Restriction map for
pFN21AE1818
(Circular) (Six-base) MAPSORT of: pFN21AE1818.seq Check: 70 from: 1 to: 5539
pFN21AE1818 5539 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3349 278
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2274 2882 1937
Size: 337 608 4594
Fragments arranged by size:
4594 608 337
Acc65I G'GTAC_C
Cuts at: 2860 2860
Size: 5539
AclI AA'CG_TT
Cuts at: 1563 3666 4039 1563
Size: 2103 373 3063
Fragments arranged by size:
3063 2103 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2228 3602 4986 5254 1632
Size: 596 1374 1384 268 1917
Fragments arranged by size:
1917 1384 1374 596 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5342
AflIII A'CryG_T
Cuts at: 1203 4438 5415 1203
Size: 3235 977 1327
Fragments arranged by size:
3235 1327 977
AgeI A'CCGG_T
Cuts at: 4370 4370
Size: 5539
AhdI GACnn_n'nnGTC
Cuts at: 4268 4268
Size: 5539
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5539
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5539
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2052 2084 2052
Size: 32 5507
AlwNI CAG_nnn'CTG
Cuts at: 2195 3282 4854 2195
Size: 1087 1572 2880
Fragments arranged by size:
2880 1572 1087
ApaLI G'TGCA_C
Cuts at: 2493 3596 4752 2493
Size: 1103 1156 3280
Fragments arranged by size:
3280 1156 1103
ApoI r'AATT_y
Cuts at: 1493 2486 2535 2848 3074 3226 5335 1493
Size: 993 49 313 226 152 2109 1697
Fragments arranged by size:
2109 1697 993 313 226 152 49
AseI AT'TA_AT
Cuts at: 160 1032 4093 160
Size: 872 3061 1606
Fragments arranged by size:
3061 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
AvaI C'yCGr_G
Cuts at: 1870 1943 2292 2864 1870
Size: 73 349 572 4545
Fragments arranged by size:
4545 572 349 73
BamHI G'GATC_C
Cuts at: 2869 3236 2869
Size: 367 5172
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2381 2814 2860 4315 618
Size: 325 217 627 594 433 46 1455 1842
Fragments arranged by size:
1842 1455 627 594 433 325 217 46
BanII G_rGCy'C
Cuts at: 1391 2410 2858 1391
Size: 1019 448 4072
Fragments arranged by size:
4072 1019 448
BbeI G_GCGC'C
Cuts at: 2818 2818
Size: 5539
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4568
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3730 3764 1903
Size: 34 50 34 1709 34 3678
Fragments arranged by size:
3678 1709 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3431 4641 3431
Size: 1210 4329
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5539
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4150 136
Size: 107 122 71 631 605 2478 1525
Fragments arranged by size:
2478 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2596 5534 2596
Size: 2938 2601
BlpI GC'TnA_GC
Cuts at: 2956 2956
Size: 5539
Bme1580I G_kGCm'C
Cuts at: 1790 2131 2482 2497 3600 4756 1790
Size: 341 351 15 1103 1156 2573
Fragments arranged by size:
2573 1156 1103 351 341 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5539
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2637 2831 3270
Size: 416 53 83 399 24 1220 194 439
Cuts at: 3270 4228 442
Size: 958 1753
Fragments arranged by size:
1753 1220 958 439 416 399 194 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5539
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2424 2424
Size: 5539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2397 4199 1239
Size: 1158 1802 2579
Fragments arranged by size:
2579 1802 1158
Bpu10I CC'TnA_GC
Cuts at: 2143 2143
Size: 5539
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2121 3664 4529 4827 2121
Size: 1543 865 298 2833
Fragments arranged by size:
2833 1543 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4202 882
Size: 617 2703 2219
Fragments arranged by size:
2703 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3330 493
Size: 711 2126 2702
Fragments arranged by size:
2702 2126 711
BsaBI GATnn'nnATC
Cuts at: 5533 5533
Size: 5539
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2574 2815 3346 3728
Size: 53 83 186 936 1041 241 531 382
Cuts at: 3728 4363 275
Size: 635 1451
Fragments arranged by size:
1451 1041 936 635 531 382 241 186
83 53
BsaWI w'CCGG_w
Cuts at: 3972 4370 4644 4791 5202 3972
Size: 398 274 147 411 4309
Fragments arranged by size:
4309 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2720 2750 1178
Size: 30 165 30 1317 30 3967
Fragments arranged by size:
3967 1317 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2273 2531 1186
Size: 1087 258 4194
Fragments arranged by size:
4194 1087 258
BseYI C'CCAG_C
Cuts at: 2175 4742 5238 2175
Size: 2567 496 2476
Fragments arranged by size:
2567 2476 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4966
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2989 3750 4778 1540
Size: 275 186 988 761 1028 2301
Fragments arranged by size:
2301 1028 988 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2410 2497 3600 3685 4756 2410
Size: 87 1103 85 1071 3193
Fragments arranged by size:
3193 1103 1071 87 85
BsmI GAATG_Cn'
Cuts at: 2446 3050 3143 2446
Size: 604 93 4842
Fragments arranged by size:
4842 604 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2131 2410 2482 2497 2858 3600 3685
Size: 399 341 279 72 15 361 742 85
Cuts at: 3685 4756 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 361 341 279 85
72 15
BspEI T'CCGG_A
Cuts at: 5202 5202
Size: 5539
BspHI T'CATG_A
Cuts at: 3428 5158 3428
Size: 1730 3809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2561 2894 844
Size: 1717 333 3489
Fragments arranged by size:
3489 1717 333
BsrBI CCG'CTC
Cuts at: 1394 1823 3426 1394
Size: 429 1603 3507
Fragments arranged by size:
3507 1603 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4034 4208 65
Size: 1183 2786 174 1396
Fragments arranged by size:
2786 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2312 2817 4183 4370 1700
Size: 110 502 505 1366 187 2869
Fragments arranged by size:
2869 1366 505 502 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5539
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5539
BssSI C'ACGA_G
Cuts at: 3599 4611 3599
Size: 1012 4527
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5539
BstBI TT'CG_AA
Cuts at: 4343 4343
Size: 5539
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5539
BstYI r'GATC_y
Cuts at: 1972 2343 2596 2869 3236 3621 3638 5090 5176
Size: 371 253 273 367 385 17 1452 86
Cuts at: 5176 5188 5534 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 371 367 346 273 253
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2399 2619 2399
Size: 220 5319
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4987
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4800
Fragments arranged by size:
4800 683 56
BtsI GCAGTG_nn'
Cuts at: 2193 3055 3849 3869 2193
Size: 862 794 20 3863
Fragments arranged by size:
3863 862 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5539
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2722 2757 529
Size: 35 2158 35 3311
Fragments arranged by size:
3311 2158 35 35
DraI TTT'AAA
Cuts at: 2843 2843
Size: 5539
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2658 4546 809
Size: 507 1342 1888 1802
Fragments arranged by size:
1888 1802 1342 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2656 2986 3875
Size: 54 1425 50 275 18 826 330 889
Cuts at: 3875 5504 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 826 330 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2986 1537
Size: 275 1174 4090
Fragments arranged by size:
4090 1174 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3469 1353
Size: 2116 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4126 4500 4646 387
Size: 1064 138 2537 374 146 1280
Fragments arranged by size:
2537 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2408 2408
Size: 5539
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2397 3602 4199 4986 5254 1239
Size: 393 596 169 1205 597 787 268 1524
Fragments arranged by size:
1524 1205 787 597 596 393 268 169
EcoRI G'AATT_C
Cuts at: 2535 2848 3226 2535
Size: 313 378 4848
Fragments arranged by size:
4848 378 313
EcoRV GAT'ATC
Cuts at: 2564 2564
Size: 5539
FspI TGC'GCA
Cuts at: 4045 4045
Size: 5539
HaeII r_GCGC'y
Cuts at: 1678 2818 4686 1678
Size: 1140 1868 2531
Fragments arranged by size:
2531 1868 1140
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 2262 2294 4180
Size: 32 135 32 599 32 59 32 1886
Cuts at: 4180 4212 4254 4286 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1886 599 135 59 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2883 3129 669
Size: 988 281 945 246 3079
Fragments arranged by size:
3079 988 945 281 246
HindIII A'AGCT_T
Cuts at: 748 2392 748
Size: 1644 3895
HpaI GTT'AAC
Cuts at: 3129 3129
Size: 5539
KasI G'GCGC_C
Cuts at: 2814 2814
Size: 5539
KpnI G_GTAC'C
Cuts at: 2864 2864
Size: 5539
MfeI C'AATT_G
Cuts at: 2748 3138 2748
Size: 390 5149
MluI A'CGCG_T
Cuts at: 5415 5415
Size: 5539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2133 4653 4837 1305
Size: 828 2520 184 2007
Fragments arranged by size:
2520 2007 828 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3717
Fragments arranged by size:
3717 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3497 3856 4015 518
Size: 648 2331 359 159 2042
Fragments arranged by size:
2331 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2955 3632 4780 5025 1606
Size: 1349 677 1148 245 2120
Fragments arranged by size:
2120 1349 1148 677 245
NarI GG'CG_CC
Cuts at: 2815 2815
Size: 5539
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4987
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5539
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5539
NotI GC'GGCC_GC
Cuts at: 2986 2986
Size: 5539
NspI r_CATG'y
Cuts at: 1132 1229 2066 2897 4442 1132
Size: 97 837 831 1545 2229
Fragments arranged by size:
2229 1545 837 831 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5539
PciI A'CATG_T
Cuts at: 4438 4438
Size: 5539
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2468 1108
Size: 1360 4179
PfoI T'CCnGG_A
Cuts at: 2647 2647
Size: 5539
PmeI GTTT'AAAC
Cuts at: 2843 2843
Size: 5539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 3640 5121 5153 3608
Size: 32 1481 32 3994
Fragments arranged by size:
3994 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5539
PsiI TTA'TAA
Cuts at: 3109 3109
Size: 5539
PstI C_TGCA'G
Cuts at: 830 2667 2891 830
Size: 1837 224 3478
Fragments arranged by size:
3478 1837 224
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5539
SacI G_AGCT'C
Cuts at: 2410 2410
Size: 5539
SalI G'TCGA_C
Cuts at: 1936 2881 1936
Size: 945 4594
SbfI CC_TGCA'GG
Cuts at: 2891 2891
Size: 5539
ScaI AGT'ACT
Cuts at: 3787 5499 3787
Size: 1712 3827
SfcI C'TryA_G
Cuts at: 826 1045 2663 2887 4022 4703 4894 826
Size: 219 1618 224 1135 681 191 1471
Fragments arranged by size:
1618 1471 1135 681 224 219 191
SfoI GGC'GCC
Cuts at: 2816 2816
Size: 5539
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5539
SmaI CCC'GGG
Cuts at: 1872 2866 1872
Size: 994 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2136 2292 3643 4544 4806 820
Size: 197 926 193 156 1351 901 262 1553
Fragments arranged by size:
1553 1351 926 901 262 197 193 156
SnaBI TAC'GTA
Cuts at: 493 3330 493
Size: 2837 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5539
SphI G_CATG'C
Cuts at: 1132 2897 1132
Size: 1765 3774
SspI AAT'ATT
Cuts at: 5 52 3463 5
Size: 47 3411 2081
Fragments arranged by size:
3411 2081 47
StuI AGG'CCT
Cuts at: 1517 2624 1517
Size: 1107 4432
StyI C'CwwG_G
Cuts at: 513 1065 2213 2978 513
Size: 552 1148 765 3074
Fragments arranged by size:
3074 1148 765 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2698 3566 3583 3736 3921 4366 325
Size: 1255 1118 868 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1118 868 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2299 3785 5497
Size: 274 80 33 51 1443 322 1486 1712
Cuts at: 5497 96
Size: 138
Fragments arranged by size:
1712 1486 1443 322 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5499 5531 5499
Size: 32 5507
Tth111I GACn'n_nGTC
Cuts at: 1316 2104 1316
Size: 788 4751
XbaI T'CTAG_A
Cuts at: 2875 2875
Size: 5539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2703 2703
Size: 5539
XhoI C'TCGA_G
Cuts at: 1943 2292 1943
Size: 349 5190
XmaI C'CCGG_G
Cuts at: 1870 2864 1870
Size: 994 4545
XmnI GAAnn'nnTTC
Cuts at: 3668 3668
Size: 5539
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3347 276
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI