Restriction map for
pFN21AE1818
(Linear) (Six-base) MAPSORT of: KIEE1818.seq Check: 5694 from: 1 to: 834
KIEE1818.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AccI GT'mk_AC
Cuts at: 0 269 834
Size: 269 565
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 834
Size: 223 611
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 47 79 834
Size: 47 32 755
Fragments arranged by size:
755 47 32
AlwNI CAG_nnn'CTG
Cuts at: 0 190 834
Size: 190 644
ApaLI G'TGCA_C
Cuts at: 0 488 834
Size: 488 346
ApoI r'AATT_y
Cuts at: 0 481 530 834
Size: 481 49 304
Fragments arranged by size:
481 304 49
AvaI C'yCGr_G
Cuts at: 0 287 834
Size: 287 547
BanI G'GyrC_C
Cuts at: 0 376 809 834
Size: 376 433 25
Fragments arranged by size:
433 376 25
BanII G_rGCy'C
Cuts at: 0 405 834
Size: 405 429
BbeI G_GCGC'C
Cuts at: 0 813 834
Size: 813 21
BglII A'GATC_T
Cuts at: 0 591 834
Size: 591 243
Bme1580I G_kGCm'C
Cuts at: 0 126 477 492 834
Size: 126 351 15 342
Fragments arranged by size:
351 342 126 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 632 834
Size: 632 202
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 419 834
Size: 419 415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 834
Size: 392 442
Bpu10I CC'TnA_GC
Cuts at: 0 138 834
Size: 138 696
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 834
Size: 116 718
BsaHI Gr'CG_yC
Cuts at: 0 569 810 834
Size: 569 241 24
Fragments arranged by size:
569 241 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 715 745 834
Size: 715 30 89
Fragments arranged by size:
715 89 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 268 526 834
Size: 268 258 308
Fragments arranged by size:
308 268 258
BseYI C'CCAG_C
Cuts at: 0 170 834
Size: 170 664
BsiHKAI G_wGCw'C
Cuts at: 0 405 492 834
Size: 405 87 342
Fragments arranged by size:
405 342 87
BsmI GAATG_Cn'
Cuts at: 0 441 834
Size: 441 393
Bsp1286I G_dGCh'C
Cuts at: 0 126 405 477 492 834
Size: 126 279 72 15 342
Fragments arranged by size:
342 279 126 72 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 556 834
Size: 556 278
BsrFI r'CCGG_y
Cuts at: 0 307 812 834
Size: 307 505 22
Fragments arranged by size:
505 307 22
BstYI r'GATC_y
Cuts at: 0 338 591 834
Size: 338 253 243
Fragments arranged by size:
338 253 243
Bsu36I CC'TnA_GG
Cuts at: 0 394 614 834
Size: 394 220 220
Fragments arranged by size:
394 220 220
BtsI GCAGTG_nn'
Cuts at: 0 188 834
Size: 188 646
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 717 752 834
Size: 717 35 82
Fragments arranged by size:
717 82 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 653 834
Size: 653 181
EaeI y'GGCC_r
Cuts at: 0 651 834
Size: 651 183
EcoICRI GAG'CTC
Cuts at: 0 403 834
Size: 403 431
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 392 834
Size: 223 169 442
Fragments arranged by size:
442 223 169
EcoRI G'AATT_C
Cuts at: 0 530 834
Size: 530 304
EcoRV GAT'ATC
Cuts at: 0 559 834
Size: 559 275
HaeII r_GCGC'y
Cuts at: 0 813 834
Size: 813 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 257 289 834
Size: 166 32 59 32 545
Fragments arranged by size:
545 166 59 32 32
HindIII A'AGCT_T
Cuts at: 0 387 834
Size: 387 447
KasI G'GCGC_C
Cuts at: 0 809 834
Size: 809 25
MfeI C'AATT_G
Cuts at: 0 743 834
Size: 743 91
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 834
Size: 128 706
NarI GG'CG_CC
Cuts at: 0 810 834
Size: 810 24
NspI r_CATG'y
Cuts at: 0 61 834
Size: 61 773
PflMI CCAn_nnn'nTGG
Cuts at: 0 463 834
Size: 463 371
PfoI T'CCnGG_A
Cuts at: 0 642 834
Size: 642 192
PstI C_TGCA'G
Cuts at: 0 662 834
Size: 662 172
SacI G_AGCT'C
Cuts at: 0 405 834
Size: 405 429
SfcI C'TryA_G
Cuts at: 0 658 834
Size: 658 176
SfoI GGC'GCC
Cuts at: 0 811 834
Size: 811 23
SmlI C'TyrA_G
Cuts at: 0 131 287 834
Size: 131 156 547
Fragments arranged by size:
547 156 131
StuI AGG'CCT
Cuts at: 0 619 834
Size: 619 215
StyI C'CwwG_G
Cuts at: 0 208 834
Size: 208 626
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 693 834
Size: 693 141
TatI w'GTAC_w
Cuts at: 0 294 834
Size: 294 540
Tth111I GACn'n_nGTC
Cuts at: 0 99 834
Size: 99 735
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 698 834
Size: 698 136
XhoI C'TCGA_G
Cuts at: 0 287 834
Size: 287 547
Enzymes that do cut:
AccI AcuI AloI AlwNI ApaLI ApoI AvaI BanI
BanII BbeI BglII Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaHI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspMI BsrFI BstYI Bsu36I BtsI CspCI DrdI EaeI
EcoICRI Eco57MI EcoRI EcoRV HaeII Hin4I HindIII KasI
MfeI MmeI NarI NspI PflMI PfoI PstI SacI
SfcI SfoI SmlI StuI StyI TaqII TatI Tth111I
XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BmtI BsaI BsaAI BsaBI BsaWI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgI BtgZI ClaI DraI DraIII EagI EarI EciI
EcoNI EcoO109I FalI FseI FspI FspAI HincII HpaI
KpnI MluI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TstI XbaI XmaI
XmnI ZraI