(Circular) (Six-base) MAPSORT of: pFN21AE0288.seq Check: 4374 from: 1 to: 5644 pFN21AE0288 5644 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2610 2987 1937 Size: 673 377 4594 Fragments arranged by size: 4594 673 377 Acc65I G'GTAC_C Cuts at: 2373 2965 2373 Size: 592 5052 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3707 5091 5359 1632 Size: 2075 1384 268 1917 Fragments arranged by size: 2075 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 4543 5520 1203 Size: 3340 977 1327 Fragments arranged by size: 3340 1327 977 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5644 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5644 AlwNI CAG_nnn'CTG Cuts at: 2395 3387 4959 2395 Size: 992 1572 3080 Fragments arranged by size: 3080 1572 992 ApaI G_GGCC'C Cuts at: 2520 2520 Size: 5644 ApaLI G'TGCA_C Cuts at: 3701 4857 3701 Size: 1156 4488 ApoI r'AATT_y Cuts at: 1493 2052 2953 3179 3331 5440 1493 Size: 559 901 226 152 2109 1697 Fragments arranged by size: 2109 1697 901 559 226 152 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2969 1870 Size: 73 1026 4545 Fragments arranged by size: 4545 1026 73 BamHI G'GATC_C Cuts at: 2462 2974 3341 2462 Size: 512 367 4765 Fragments arranged by size: 4765 512 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2373 2709 2965 4420 618 Size: 325 217 627 586 336 256 1455 1842 Fragments arranged by size: 1842 1455 627 586 336 325 256 217 BanII G_rGCy'C Cuts at: 1391 2479 2520 2737 2963 1391 Size: 1088 41 217 226 4072 Fragments arranged by size: 4072 1088 226 217 41 BbeI G_GCGC'C Cuts at: 2713 2713 Size: 5644 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4673 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3835 3869 1903 Size: 34 1898 34 3678 Fragments arranged by size: 3678 1898 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2070 3536 4746 2070 Size: 1466 1210 2968 Fragments arranged by size: 2968 1466 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2456 4255 136 Size: 107 122 71 631 605 784 1799 1525 Fragments arranged by size: 1799 1525 784 631 605 122 107 71 BglII A'GATC_T Cuts at: 5639 5639 Size: 5644 BlpI GC'TnA_GC Cuts at: 2421 2426 3061 2421 Size: 5 635 5004 Fragments arranged by size: 5004 635 5 Bme1580I G_kGCm'C Cuts at: 1790 2520 2769 3705 4861 1790 Size: 730 249 936 1156 2573 Fragments arranged by size: 2573 1156 936 730 249 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2332 3375 4333 Size: 416 53 83 399 24 915 1043 958 Cuts at: 4333 442 Size: 1753 Fragments arranged by size: 1753 1043 958 915 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2465 2466 4304 1239 Size: 1226 1 1838 2579 Fragments arranged by size: 2579 1838 1226 1 Bpu10I CC'TnA_GC Cuts at: 2072 2072 Size: 5644 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3769 4634 4932 3769 Size: 865 298 4481 Fragments arranged by size: 4481 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4307 882 Size: 617 2808 2219 Fragments arranged by size: 2808 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3435 493 Size: 711 2231 2702 Fragments arranged by size: 2702 2231 711 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2710 3451 3833 4468 Size: 53 83 186 936 1177 741 382 635 Cuts at: 4468 275 Size: 1451 Fragments arranged by size: 1451 1177 936 741 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4077 4475 4749 4896 5307 4077 Size: 398 274 147 411 4414 Fragments arranged by size: 4414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2724 2754 1178 Size: 30 165 30 1321 30 4068 Fragments arranged by size: 4068 1321 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2218 2642 1186 Size: 1032 424 4188 Fragments arranged by size: 4188 1032 424 BseYI C'CCAG_C Cuts at: 2122 4847 5343 2122 Size: 2725 496 2423 Fragments arranged by size: 2725 2423 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5071 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3094 3855 4883 1540 Size: 275 186 1093 761 1028 2301 Fragments arranged by size: 2301 1093 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2412 3705 3790 4861 2412 Size: 1293 85 1071 3195 Fragments arranged by size: 3195 1293 1071 85 BsmI GAATG_Cn' Cuts at: 2916 3155 3248 2916 Size: 239 93 5312 Fragments arranged by size: 5312 239 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2412 2479 2520 2737 2769 2963 3705 Size: 399 622 67 41 217 32 194 742 Cuts at: 3705 3790 4861 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 622 399 217 194 85 67 41 32 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 2250 2412 3533 5263 2250 Size: 162 1121 1730 2631 Fragments arranged by size: 2631 1730 1121 162 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2139 2999 844 Size: 1295 860 3489 Fragments arranged by size: 3489 1295 860 BsrBI CCG'CTC Cuts at: 1394 1823 3531 1394 Size: 429 1708 3507 Fragments arranged by size: 3507 1708 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4139 4313 65 Size: 1183 2891 174 1396 Fragments arranged by size: 2891 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4288 4475 1700 Size: 110 2478 187 2869 Fragments arranged by size: 2869 2478 187 110 BsrGI T'GTAC_A Cuts at: 96 2184 96 Size: 2088 3556 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5644 BssSI C'ACGA_G Cuts at: 3704 4716 3704 Size: 1012 4632 BstAPI GCAn_nnn'nTGC Cuts at: 794 2340 794 Size: 1546 4098 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 2749 1184 Size: 1565 4079 BstXI CCAn_nnnn'nTGG Cuts at: 2527 2527 Size: 5644 BstYI r'GATC_y Cuts at: 1972 2462 2974 3341 3726 3743 5195 5281 5293 Size: 490 512 367 385 17 1452 86 12 Cuts at: 5293 5639 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 512 490 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2786 2786 Size: 5644 BtgI C'CryG_G Cuts at: 513 1065 2004 2470 2745 2835 513 Size: 552 939 466 275 90 3322 Fragments arranged by size: 3322 939 552 466 275 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4905 Fragments arranged by size: 4905 683 56 BtsI GCAGTG_nn' Cuts at: 2562 3160 3954 3974 2562 Size: 598 794 20 4232 Fragments arranged by size: 4232 794 598 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DraIII CAC_nnn'GTG Cuts at: 2340 2340 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2585 4651 809 Size: 507 1269 2066 1802 Fragments arranged by size: 2066 1802 1269 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2838 3091 3980 Size: 54 1425 50 275 18 1008 253 889 Cuts at: 3980 5609 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1008 889 275 253 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3091 1537 Size: 275 1279 4090 Fragments arranged by size: 4090 1279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3574 1353 Size: 2221 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2489 4231 4605 4751 387 Size: 1064 138 900 1742 374 146 1280 Fragments arranged by size: 1742 1280 1064 900 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2465 2466 3707 4304 5091 5359 1239 Size: 393 833 1 1241 597 787 268 1524 Fragments arranged by size: 1524 1241 833 787 597 393 268 1 EcoNI CCTnn'n_nnAGG Cuts at: 2368 2368 Size: 5644 EcoRI G'AATT_C Cuts at: 2052 2953 3331 2052 Size: 901 378 4365 Fragments arranged by size: 4365 901 378 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 2433 2713 4791 1678 Size: 755 280 2078 2531 Fragments arranged by size: 2531 2078 755 280 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4285 4317 4359 4391 1373 Size: 32 135 32 2713 32 42 32 2626 Fragments arranged by size: 2713 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2031 2590 2988 3234 669 Size: 988 281 93 559 398 246 3079 Fragments arranged by size: 3079 988 559 398 281 246 93 HindIII A'AGCT_T Cuts at: 748 748 Size: 5644 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KasI G'GCGC_C Cuts at: 2709 2709 Size: 5644 KpnI G_GTAC'C Cuts at: 2377 2969 2377 Size: 592 5052 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4758 4942 1305 Size: 3453 184 2007 Fragments arranged by size: 3453 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3822 Fragments arranged by size: 3822 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2216 2357 2618 3602 3961 4120 518 Size: 648 1050 141 261 984 359 159 2042 Fragments arranged by size: 2042 1050 984 648 359 261 159 141 MspA1I CmG'CkG Cuts at: 1606 2122 2699 2822 3060 3737 4885 5130 1606 Size: 516 577 123 238 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 577 516 245 238 123 NarI GG'CG_CC Cuts at: 2710 2710 Size: 5644 NcoI C'CATG_G Cuts at: 513 1065 2004 2470 513 Size: 552 939 466 3687 Fragments arranged by size: 3687 939 552 466 NdeI CA'TA_TG Cuts at: 387 387 Size: 5644 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 3002 4547 1132 Size: 97 1773 1545 2229 Fragments arranged by size: 2229 1773 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5644 PciI A'CATG_T Cuts at: 4543 4543 Size: 5644 PflMI CCAn_nnn'nTGG Cuts at: 1108 2291 1108 Size: 1183 4461 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PspOMI G'GGCC_C Cuts at: 2516 2516 Size: 5644 PstI C_TGCA'G Cuts at: 830 2557 2996 830 Size: 1727 439 3478 Fragments arranged by size: 3478 1727 439 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 PvuII CAG'CTG Cuts at: 2122 2699 2822 2122 Size: 577 123 4944 Fragments arranged by size: 4944 577 123 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 3892 5604 3892 Size: 1712 3932 SfcI C'TryA_G Cuts at: 826 1045 2553 2992 4127 4808 4999 826 Size: 219 1508 439 1135 681 191 1471 Fragments arranged by size: 1508 1471 1135 681 439 219 191 SfoI GGC'GCC Cuts at: 2711 2711 Size: 5644 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2971 1872 Size: 1099 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3748 4649 4911 820 Size: 197 926 1805 901 262 1553 Fragments arranged by size: 1805 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 3002 1132 Size: 1870 3774 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 2791 1517 Size: 1274 4370 StyI C'CwwG_G Cuts at: 513 1065 2004 2162 2470 3083 513 Size: 552 939 158 308 613 3074 Fragments arranged by size: 3074 939 613 552 308 158 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2351 3671 3688 3841 4026 4471 325 Size: 1255 771 1320 17 153 185 445 1498 Fragments arranged by size: 1498 1320 1255 771 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 2757 3890 Size: 274 80 33 51 1443 207 573 1133 Cuts at: 3890 5602 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1133 573 274 207 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5604 5636 5604 Size: 32 5612 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5644 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2699 2806 2699 Size: 107 5537 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2969 1870 Size: 1099 4545 XmnI GAAnn'nnTTC Cuts at: 3773 3773 Size: 5644 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI