##################################
# NO CUT ENZYMES (  217 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AcyI        AflII       AgeI        AhdI        
AluI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmBI       BsmFI       BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MaeI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspA1I      MspI        
MunI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tru9I       Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AclI           92  AACGTT               AA^CGTT
AcsI          164  AAATTT               [GA]^AATT[CT]
AfaI           62  GTAC                 GT^AC
AflIII         58  ACATGT               A^C[GA][CT]GT
AlwI            5  GGATC                GGATC(4/5)
ApoI          164  AAATTT               [GA]^AATT[CT]
BbvI          170  GCTGC                GCAGC(8/12)
BsaJI          65  CCTTGG               C^C[ACGT][ACGT]GG
BsgI          171  CTGCAC               GTGCAG(16/14)
BsiEI          99  CGGTCG               CG[GA][CT]^CG
BsmAI         113  GAGAC                GTCTC(1/5)
BsmI           72  GAATGC               GAATGC(1/-1)
BspLU11I       58  ACATGT               A^CATGT
BstYI           5  GGATCT               [GA]^GATC[CT]
DdeI          176  CTGAG                C^T[ACGT]AG
EcoT14I        65  CCTTGG               C^C[AT][AT]GG
Fnu4HI        170  GCTGC                GC^[ACGT]GC
HphI           69  GGTGA                GGTGA(8/7)
ItaI          170  GCTGC                GC^[ACGT]GC
MaeII          93  ACGT                 A^CGT
MflI            5  GGATCT               [GA]^GATC[CT]
MnlI            3  GAGG                 CCTC(7/6)
NdeI          144  CATATG               CA^TATG
NlaIII         59  CATG                 CATG^
NspI           58  ACATGT               [GA]CATG^[CT]
Psp1406I       92  AACGTT               AA^CGTT
RsaI           62  GTAC                 GT^AC
SmlI          121  CTTGAG               C^T[CT][GA]AG
StyI           65  CCTTGG               C^C[AT][AT]GG
TseI          170  GCTGC                G^C[AT]GC
Tsp45I         37  GTGAC                ^GT[GC]AC
TspRI         174  CACTG                CA[GC]TG[ACGT][ACGT]^
XcmI           56  CCACATGTACCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII           5  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

DpnII           6  GATC                 ^GATC
               79  GATC                
MaeIII         37  GTGAC                ^GT[ACGT]AC
               95  GTTAC               
MboI            6  GATC                 ^GATC
               79  GATC                
MboII         190  GAAGA                GAAGA(8/7)
              193  GAAGA               
NdeII           6  GATC                 ^GATC
               79  GATC                
Sau3AI          6  GATC                 ^GATC
               79  GATC                
Tsp509I       165  AATT                 ^AATT
              182  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    0 enzymes) #
#########################################