(Circular) (Six-base) MAPSORT of: pFN21AE0286.seq Check: 4361 from: 1 to: 4912 pFN21AE0286 4912 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2722 278 Size: 53 83 186 936 1186 2468 Fragments arranged by size: 2468 1186 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2255 1937 Size: 318 4594 Acc65I G'GTAC_C Cuts at: 2233 2233 Size: 4912 AclI AA'CG_TT Cuts at: 1563 2098 3039 3412 1563 Size: 535 941 373 3063 Fragments arranged by size: 3063 941 535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2975 4359 4627 1632 Size: 1343 1384 268 1917 Fragments arranged by size: 1917 1384 1343 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4715 AflIII A'CryG_T Cuts at: 1203 2063 3811 4788 1203 Size: 860 1748 977 1327 Fragments arranged by size: 1748 1327 977 860 AgeI A'CCGG_T Cuts at: 3743 3743 Size: 4912 AhdI GACnn_n'nnGTC Cuts at: 3641 3641 Size: 4912 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4912 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4912 AlwNI CAG_nnn'CTG Cuts at: 2655 4227 2655 Size: 1572 3340 ApaLI G'TGCA_C Cuts at: 2969 4125 2969 Size: 1156 3756 ApoI r'AATT_y Cuts at: 1493 2169 2221 2447 2599 4708 1493 Size: 676 52 226 152 2109 1697 Fragments arranged by size: 2109 1697 676 226 152 52 AseI AT'TA_AT Cuts at: 160 1032 3466 160 Size: 872 2434 1606 Fragments arranged by size: 2434 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4912 AvaI C'yCGr_G Cuts at: 1870 1943 2237 1870 Size: 73 294 4545 Fragments arranged by size: 4545 294 73 BamHI G'GATC_C Cuts at: 2242 2609 2242 Size: 367 4545 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2233 3688 618 Size: 325 217 627 446 1455 1842 Fragments arranged by size: 1842 1455 627 446 325 217 BanII G_rGCy'C Cuts at: 1391 2231 1391 Size: 840 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3941 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3103 3137 1903 Size: 34 1166 34 3678 Fragments arranged by size: 3678 1166 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2804 4014 2804 Size: 1210 3702 BclI T'GATC_A Cuts at: 1548 1548 Size: 4912 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3523 136 Size: 107 122 71 631 605 1851 1525 Fragments arranged by size: 1851 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4907 4907 Size: 4912 BlpI GC'TnA_GC Cuts at: 2329 2329 Size: 4912 Bme1580I G_kGCm'C Cuts at: 1790 2973 4129 1790 Size: 1183 1156 2573 Fragments arranged by size: 2573 1183 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4912 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2643 3601 442 Size: 416 53 83 399 24 1226 958 1753 Fragments arranged by size: 1753 1226 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4912 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3572 1239 Size: 2333 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2147 3037 3902 4200 2147 Size: 890 865 298 2859 Fragments arranged by size: 2859 890 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3575 882 Size: 617 2076 2219 Fragments arranged by size: 2219 2076 617 BsaAI yAC'GTr Cuts at: 493 1204 2703 493 Size: 711 1499 2702 Fragments arranged by size: 2702 1499 711 BsaBI GATnn'nnATC Cuts at: 4906 4906 Size: 4912 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2719 3101 3736 275 Size: 53 83 186 936 1186 382 635 1451 Fragments arranged by size: 1451 1186 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3345 3743 4017 4164 4575 3345 Size: 398 274 147 411 3682 Fragments arranged by size: 3682 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4687 Fragments arranged by size: 4687 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4912 BseYI C'CCAG_C Cuts at: 4115 4611 4115 Size: 496 4416 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2161 1163 Size: 573 425 3914 Fragments arranged by size: 3914 573 425 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2107 2362 3123 4151 1540 Size: 275 186 106 255 761 1028 2301 Fragments arranged by size: 2301 1028 761 275 255 186 106 BsiHKAI G_wGCw'C Cuts at: 2973 3058 4129 2973 Size: 85 1071 3756 Fragments arranged by size: 3756 1071 85 BsmI GAATG_Cn' Cuts at: 2083 2423 2516 2083 Size: 340 93 4479 Fragments arranged by size: 4479 340 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2231 2973 3058 4129 1391 Size: 399 441 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 441 399 85 BspEI T'CCGG_A Cuts at: 4575 4575 Size: 4912 BspHI T'CATG_A Cuts at: 2801 4531 2801 Size: 1730 3182 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2267 844 Size: 1423 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2799 1394 Size: 429 976 3507 Fragments arranged by size: 3507 976 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3407 3581 65 Size: 1183 2159 174 1396 Fragments arranged by size: 2159 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3556 3743 1700 Size: 110 1746 187 2869 Fragments arranged by size: 2869 1746 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4912 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4912 BssSI C'ACGA_G Cuts at: 2972 3984 2972 Size: 1012 3900 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4912 BstBI TT'CG_AA Cuts at: 3716 3716 Size: 4912 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4912 BstYI r'GATC_y Cuts at: 1972 2010 2242 2609 2994 3011 4463 4549 4561 Size: 38 232 367 385 17 1452 86 12 Cuts at: 4561 4907 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 232 86 38 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4360 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4173 Fragments arranged by size: 4173 683 56 BtsI GCAGTG_nn' Cuts at: 2428 3222 3242 2428 Size: 794 20 4098 Fragments arranged by size: 4098 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4912 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4877 DraI TTT'AAA Cuts at: 2216 2216 Size: 4912 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3919 809 Size: 507 2603 1802 Fragments arranged by size: 2603 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2359 3248 4877 Size: 54 1425 50 275 18 529 889 1629 Cuts at: 4877 8 Size: 43 Fragments arranged by size: 1629 1425 889 529 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2359 1537 Size: 275 547 4090 Fragments arranged by size: 4090 547 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2842 1353 Size: 1489 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3499 3873 4019 387 Size: 1064 138 1910 374 146 1280 Fragments arranged by size: 1910 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2975 3572 4359 4627 1239 Size: 393 1343 597 787 268 1524 Fragments arranged by size: 1524 1343 787 597 393 268 EcoRI G'AATT_C Cuts at: 2221 2599 2221 Size: 378 4534 FspI TGC'GCA Cuts at: 3418 3418 Size: 4912 HaeII r_GCGC'y Cuts at: 1678 4059 1678 Size: 2381 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3553 3585 3627 3659 1373 Size: 32 135 32 1981 32 42 32 2626 Fragments arranged by size: 2626 1981 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2256 2502 669 Size: 988 281 318 246 3079 Fragments arranged by size: 3079 988 318 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4912 HpaI GTT'AAC Cuts at: 2502 2502 Size: 4912 KpnI G_GTAC'C Cuts at: 2237 2237 Size: 4912 MfeI C'AATT_G Cuts at: 2511 2511 Size: 4912 MluI A'CGCG_T Cuts at: 4788 4788 Size: 4912 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4026 4210 1305 Size: 2721 184 2007 Fragments arranged by size: 2721 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3090 Fragments arranged by size: 3090 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2870 3229 3388 518 Size: 648 1704 359 159 2042 Fragments arranged by size: 2042 1704 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2328 3005 4153 4398 1606 Size: 722 677 1148 245 2120 Fragments arranged by size: 2120 1148 722 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4360 NdeI CA'TA_TG Cuts at: 387 2150 387 Size: 1763 3149 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4912 NotI GC'GGCC_GC Cuts at: 2359 2359 Size: 4912 NspI r_CATG'y Cuts at: 1132 1229 2067 2270 3815 1132 Size: 97 838 203 1545 2229 Fragments arranged by size: 2229 1545 838 203 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4912 PciI A'CATG_T Cuts at: 2063 3811 2063 Size: 1748 3164 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4912 PmeI GTTT'AAAC Cuts at: 2216 2216 Size: 4912 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2981 3013 4494 4526 2981 Size: 32 1481 32 3367 Fragments arranged by size: 3367 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4912 PsiI TTA'TAA Cuts at: 2482 2482 Size: 4912 PstI C_TGCA'G Cuts at: 830 2264 830 Size: 1434 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4912 SalI G'TCGA_C Cuts at: 1936 2254 1936 Size: 318 4594 SbfI CC_TGCA'GG Cuts at: 2264 2264 Size: 4912 ScaI AGT'ACT Cuts at: 3160 4872 3160 Size: 1712 3200 SfcI C'TryA_G Cuts at: 826 1045 2260 3395 4076 4267 826 Size: 219 1215 1135 681 191 1471 Fragments arranged by size: 1471 1215 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4912 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4912 SmaI CCC'GGG Cuts at: 1872 2239 1872 Size: 367 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2126 3016 3917 4179 820 Size: 197 926 183 890 901 262 1553 Fragments arranged by size: 1553 926 901 890 262 197 183 SnaBI TAC'GTA Cuts at: 493 2703 493 Size: 2210 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4912 SphI G_CATG'C Cuts at: 1132 2270 1132 Size: 1138 3774 SspI AAT'ATT Cuts at: 5 52 2836 5 Size: 47 2784 2081 Fragments arranged by size: 2784 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4912 StyI C'CwwG_G Cuts at: 513 1065 2070 2351 513 Size: 552 1005 281 3074 Fragments arranged by size: 3074 1005 552 281 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2939 2956 3109 3294 3739 325 Size: 1255 1359 17 153 185 445 1498 Fragments arranged by size: 1498 1359 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3158 4870 96 Size: 274 80 33 51 1443 1181 1712 138 Fragments arranged by size: 1712 1443 1181 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4872 4904 4872 Size: 32 4880 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4912 XbaI T'CTAG_A Cuts at: 2248 2248 Size: 4912 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2068 2068 Size: 4912 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4912 XmaI C'CCGG_G Cuts at: 1870 2237 1870 Size: 367 4545 XmnI GAAnn'nnTTC Cuts at: 3041 3041 Size: 4912 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2720 276 Size: 53 83 186 936 1186 2468 Fragments arranged by size: 2468 1186 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI