Restriction map for
pFN21AE0284
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI ApoI AscI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI BspDI BspMI BsrBI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstZ17I CelII ClaI CpoI DraI
DraIII EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MluNI MscI
MunI MvnI NarI NdeI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 75 enzymes) #
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AccI 543 GTCTAC GT^[AC][GT]AC
AccIII 336 TCCGGA T^CCGGA
AflIII 554 ACATGT A^C[GA][CT]GT
AlwI 703 GATCC GGATC(4/5)
AocI 484 CCTCAGG CC^T[ACGT]AGG
ApaI 108 GGGCCC GGGCC^C
AseI 817 ATTAAT AT^TAAT
AsnI 817 ATTAAT AT^TAAT
Asp700I 1002 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 604 GAGCTC G[AT]GC[AT]^C
AvaII 527 GGACC G^G[AT]CC
BbrPI 459 CACGTG CAC^GTG
BbsI 960 GAAGAC GAAGAC(2/6)
BbvCI 891 CCTCAGC CCTCAGC(-5/-2)
BpuAI 960 GAAGAC GAAGAC(2/6)
BsaAI 459 CACGTG [CT]AC^GT[GA]
BsaI 60 GGTCTC GGTCTC(1/5)
BsaWI 336 TCCGGA [AT]^CCGG[AT]
BseAI 336 TCCGGA T^CCGGA
BsiHKAI 604 GAGCTC G[AT]GC[AT]^C
BsmFI 743 GGGAC GGGAC(10/14)
Bsp1407I 598 TGTACA T^GTACA
BspEI 336 TCCGGA T^CCGGA
BspHI 956 TCATGA T^CATGA
BspLU11I 554 ACATGT A^CATGT
BsrGI 598 TGTACA T^GTACA
BstAPI 651 GCAGCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 196 TTCGAA TT^CGAA
BstYI 702 AGATCC [GA]^GATC[CT]
Bsu36I 484 CCTCAGG CC^T[ACGT]AGG
CfoI 386 GCGC GCG^C
DpnII 703 GATC ^GATC
DraII 887 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 745 GACTTAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 522 TGGCCG [CT]^GGCC[GA]
Eco81I 484 CCTCAGG CC^T[ACGT]AGG
EcoO109I 887 AGGGCCT [GA]G^G[ACGT]CC[CT]
FseI 910 GGCCGGCC GGCCGG^CC
HhaI 386 GCGC GCG^C
HinP1I 386 GCGC G^CGC
MaeIII 727 GTCAC ^GT[ACGT]AC
MboI 703 GATC ^GATC
MflI 702 AGATCC [GA]^GATC[CT]
MroI 336 TCCGGA T^CCGGA
MslI 553 CACATGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 911 GCCGGC GCC^GGC
NdeII 703 GATC ^GATC
NgoMIV 911 GCCGGC G^CCGGC
NheI 237 GCTAGC G^CTAGC
NspI 554 ACATGT [GA]CATG^[CT]
NspV 196 TTCGAA TT^CGAA
PflMI 774 CCAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 541 GAGTC GAGTC(4/5)
PmaCI 459 CACGTG CAC^GTG
PmlI 459 CACGTG CAC^GTG
PshBI 817 ATTAAT AT^TAAT
PstI 649 CTGCAG CTGCA^G
PvuII 176 CAGCTG CAG^CTG
RcaI 956 TCATGA T^CATGA
SacI 604 GAGCTC GAGCT^C
Sau3AI 703 GATC ^GATC
ScaI 776 AGTACT AGT^ACT
SfuI 196 TTCGAA TT^CGAA
SmaI 130 CCCGGG CCC^GGG
SspBI 598 TGTACA T^GTACA
StuI 693 AGGCCT AGG^CCT
TfiI 477 GATTC G^A[AT]TC
Tsp45I 727 GTCAC ^GT[GC]AC
Tsp509I 720 AATT ^AATT
Van91I 774 CCAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 873 TCTAGA T^CTAGA
XhoII 702 AGATCC [GA]^GATC[CT]
XmaCI 130 CCCGGG C^CCGGG
XmaI 130 CCCGGG C^CCGGG
XmnI 1002 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 599 GTAC GT^AC
777 GTAC
AvaI 111 CCCGAG C^[CT]CG[GA]G
130 CCCGGG
BfaI 238 CTAG C^TAG
874 CTAG
BpmI 254 CTGGAG CTGGAG(16/14)
661 CTGGAG
BsmAI 737 GAGAC GTCTC(1/5)
61 GTCTC
BsoBI 111 CCCGAG C^[CT]CG[GA]G
130 CCCGGG
BsrDI 945 CATTGC GCAATG(2/0)
400 GCAATG
BsrFI 181 GCCGGT [GA]^CCGG[CT]
911 GCCGGC
BstXI 359 CCAGAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
375 CCACGGCTTTGG
Cfr10I 181 GCCGGT [GA]^CCGG[CT]
911 GCCGGC
HinfI 477 GATTC G^A[ACGT]TC
541 GAGTC
HphI 608 TCACC GGTGA(8/7)
728 TCACC
MaeI 238 CTAG C^TAG
874 CTAG
MaeII 460 ACGT A^CGT
710 ACGT
MseI 748 TTAA T^TAA
818 TTAA
RsaI 599 GTAC GT^AC
777 GTAC
SfcI 569 CTGTAG C^T[GA][CT]AG
649 CTGCAG
Tru9I 748 TTAA T^TAA
818 TTAA
XcmI 356 CCACCAGAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
392 CCATGGCGGCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DsaI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HpaII ItaI MboII MnlI MspA1I
MspI MvaI MwoI NciI NcoI
NlaIII NlaIV Sau96I ScrFI SfaNI
StyI TaqI TseI TspRI TthHB8I