(Circular) (Six-base) MAPSORT of: pFN21AE0284.seq Check: 1203 from: 1 to: 5716 pFN21AE0284 5716 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3526 278 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2549 3059 1937 Size: 612 510 4594 Fragments arranged by size: 4594 612 510 Acc65I G'GTAC_C Cuts at: 3037 3037 Size: 5716 AclI AA'CG_TT Cuts at: 1563 3843 4216 1563 Size: 2280 373 3063 Fragments arranged by size: 3063 2280 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2154 3779 5163 5431 1632 Size: 522 1625 1384 268 1917 Fragments arranged by size: 1917 1625 1384 522 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5519 AflIII A'CryG_T Cuts at: 1203 2559 4615 5592 1203 Size: 1356 2056 977 1327 Fragments arranged by size: 2056 1356 1327 977 AgeI A'CCGG_T Cuts at: 4547 4547 Size: 5716 AhdI GACnn_n'nnGTC Cuts at: 4445 4445 Size: 5716 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5716 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5716 AlwNI CAG_nnn'CTG Cuts at: 3459 5031 3459 Size: 1572 4144 ApaI G_GGCC'C Cuts at: 2117 2117 Size: 5716 ApaLI G'TGCA_C Cuts at: 3773 4929 3773 Size: 1156 4560 ApoI r'AATT_y Cuts at: 1493 3025 3251 3403 5512 1493 Size: 1532 226 152 2109 1697 Fragments arranged by size: 2109 1697 1532 226 152 AseI AT'TA_AT Cuts at: 160 1032 2823 4270 160 Size: 872 1791 1447 1606 Fragments arranged by size: 1791 1606 1447 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 AvaI C'yCGr_G Cuts at: 1870 1943 2116 2135 3041 1870 Size: 73 173 19 906 4545 Fragments arranged by size: 4545 906 173 73 19 BamHI G'GATC_C Cuts at: 3046 3413 3046 Size: 367 5349 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3037 4492 618 Size: 325 217 627 1250 1455 1842 Fragments arranged by size: 1842 1455 1250 627 325 217 BanII G_rGCy'C Cuts at: 1391 2117 2315 2613 3035 1391 Size: 726 198 298 422 4072 Fragments arranged by size: 4072 726 422 298 198 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2972 928 Size: 971 1073 3672 Fragments arranged by size: 3672 1073 971 BbvCI CC'TCA_GC Cuts at: 2897 2897 Size: 5716 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3907 3941 1903 Size: 34 1970 34 3678 Fragments arranged by size: 3678 1970 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3608 4818 3608 Size: 1210 4506 BclI T'GATC_A Cuts at: 1548 1548 Size: 5716 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4327 136 Size: 107 122 71 631 605 2655 1525 Fragments arranged by size: 2655 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5711 5711 Size: 5716 BlpI GC'TnA_GC Cuts at: 3133 3133 Size: 5716 Bme1580I G_kGCm'C Cuts at: 1790 2117 3777 4933 1790 Size: 327 1660 1156 2573 Fragments arranged by size: 2573 1660 1156 327 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5716 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2755 3447 4405 Size: 416 53 83 399 24 1338 692 958 Cuts at: 4405 442 Size: 1753 Fragments arranged by size: 1753 1338 958 692 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2246 1056 Size: 1190 4526 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2368 2368 Size: 5716 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2280 2687 4376 1239 Size: 1041 407 1689 2579 Fragments arranged by size: 2579 1689 1041 407 Bpu10I CC'TnA_GC Cuts at: 2897 2897 Size: 5716 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3841 4706 5004 3841 Size: 865 298 4553 Fragments arranged by size: 4553 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2071 4379 882 Size: 617 572 2308 2219 Fragments arranged by size: 2308 2219 617 572 BsaAI yAC'GTr Cuts at: 493 1204 2466 3507 493 Size: 711 1262 1041 2702 Fragments arranged by size: 2702 1262 1041 711 BsaBI GATnn'nnATC Cuts at: 5710 5710 Size: 5716 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3523 3905 4540 275 Size: 53 83 186 936 1990 382 635 1451 Fragments arranged by size: 1990 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2341 4149 4547 4821 4968 5379 2341 Size: 1808 398 274 147 411 2678 Fragments arranged by size: 2678 1808 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2002 2032 2049 2079 2531 Size: 30 165 30 599 30 17 30 452 Cuts at: 2531 2561 1178 Size: 30 4333 Fragments arranged by size: 4333 599 452 165 30 30 30 30 30 17 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5716 BseYI C'CCAG_C Cuts at: 2179 2308 4919 5415 2179 Size: 129 2611 496 2480 Fragments arranged by size: 2611 2480 496 129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5143 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3166 3927 4955 1540 Size: 275 186 1165 761 1028 2301 Fragments arranged by size: 2301 1165 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2613 3777 3862 4933 2613 Size: 1164 85 1071 3396 Fragments arranged by size: 3396 1164 1071 85 BsmI GAATG_Cn' Cuts at: 3227 3320 3227 Size: 93 5623 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2117 2315 2613 3035 3777 3862 4933 Size: 399 327 198 298 422 742 85 1071 Cuts at: 4933 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 422 399 327 298 198 85 BspEI T'CCGG_A Cuts at: 2341 5379 2341 Size: 3038 2678 BspHI T'CATG_A Cuts at: 2961 3605 5335 2961 Size: 644 1730 3342 Fragments arranged by size: 3342 1730 644 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3071 844 Size: 2227 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3603 1394 Size: 429 1780 3507 Fragments arranged by size: 3507 1780 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2412 2949 4211 4385 65 Size: 1183 1164 537 1262 174 1396 Fragments arranged by size: 1396 1262 1183 1164 537 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2186 2916 4360 4547 1700 Size: 110 376 730 1444 187 2869 Fragments arranged by size: 2869 1444 730 376 187 110 BsrGI T'GTAC_A Cuts at: 96 2603 96 Size: 2507 3209 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5716 BssSI C'ACGA_G Cuts at: 3776 4788 3776 Size: 1012 4704 BstAPI GCAn_nnn'nTGC Cuts at: 794 2662 794 Size: 1868 3848 BstBI TT'CG_AA Cuts at: 2202 4520 2202 Size: 2318 3398 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5716 BstXI CCAn_nnnn'nTGG Cuts at: 2371 2387 2371 Size: 16 5700 BstYI r'GATC_y Cuts at: 1972 2707 3046 3413 3798 3815 5267 5353 5365 Size: 735 339 367 385 17 1452 86 12 Cuts at: 5365 5711 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 735 385 367 346 339 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2490 2490 Size: 5716 BtgI C'CryG_G Cuts at: 513 1065 2004 2380 2397 2535 2631 2850 513 Size: 552 939 376 17 138 96 219 3379 Fragments arranged by size: 3379 939 552 376 219 138 96 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4977 Fragments arranged by size: 4977 683 56 BtsI GCAGTG_nn' Cuts at: 2822 3232 4026 4046 2822 Size: 410 794 20 4492 Fragments arranged by size: 4492 794 410 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5716 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5681 DraI TTT'AAA Cuts at: 3020 3020 Size: 5716 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2756 4723 809 Size: 507 1440 1967 1802 Fragments arranged by size: 1967 1802 1440 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2527 3163 4052 Size: 54 1425 50 275 18 697 636 889 Cuts at: 4052 5681 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 697 636 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3163 1537 Size: 275 1351 4090 Fragments arranged by size: 4090 1351 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3646 1353 Size: 2293 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2816 4303 4677 4823 387 Size: 1064 138 1227 1487 374 146 1280 Fragments arranged by size: 1487 1280 1227 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2611 2611 Size: 5716 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2154 2280 2687 3779 4376 5163 5431 Size: 393 522 126 407 1092 597 787 268 Cuts at: 5431 1239 Size: 1524 Fragments arranged by size: 1524 1092 787 597 522 407 393 268 126 EcoO109I rG'GnC_Cy Cuts at: 2893 2893 Size: 5716 EcoRI G'AATT_C Cuts at: 3025 3403 3025 Size: 378 5338 FseI GG_CCGG'CC Cuts at: 2920 2920 Size: 5716 FspI TGC'GCA Cuts at: 4222 4222 Size: 5716 HaeII r_GCGC'y Cuts at: 1678 4863 1678 Size: 3185 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2002 2034 2049 2081 2473 Size: 32 135 32 430 32 15 32 392 Cuts at: 2473 2505 2531 2563 2973 3005 4357 4389 4431 Size: 32 26 32 410 32 1352 32 42 Cuts at: 4431 4463 1373 Size: 32 2626 Fragments arranged by size: 2626 1352 430 410 392 135 42 32 32 32 32 32 32 32 32 32 26 15 HincII GTy'rAC Cuts at: 669 1657 1938 3060 3306 669 Size: 988 281 1122 246 3079 Fragments arranged by size: 3079 1122 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5716 HpaI GTT'AAC Cuts at: 3306 3306 Size: 5716 KpnI G_GTAC'C Cuts at: 3041 3041 Size: 5716 MfeI C'AATT_G Cuts at: 3315 3315 Size: 5716 MluI A'CGCG_T Cuts at: 5592 5592 Size: 5716 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4830 5014 1305 Size: 3525 184 2007 Fragments arranged by size: 3525 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3894 Fragments arranged by size: 3894 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2562 3674 4033 4192 518 Size: 648 1396 1112 359 159 2042 Fragments arranged by size: 2042 1396 1112 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2024 2183 2928 3132 3809 4957 5202 1606 Size: 418 159 745 204 677 1148 245 2120 Fragments arranged by size: 2120 1148 745 677 418 245 204 159 NaeI GCC'GGC Cuts at: 2918 2918 Size: 5716 NcoI C'CATG_G Cuts at: 513 1065 2004 2397 2535 2631 2850 513 Size: 552 939 393 138 96 219 3379 Fragments arranged by size: 3379 939 552 393 219 138 96 NdeI CA'TA_TG Cuts at: 387 387 Size: 5716 NgoMIV G'CCGG_C Cuts at: 2916 2916 Size: 5716 NheI G'CTAG_C Cuts at: 1052 2242 1052 Size: 1190 4526 NotI GC'GGCC_GC Cuts at: 3163 3163 Size: 5716 NspI r_CATG'y Cuts at: 1132 1229 2563 3074 4619 1132 Size: 97 1334 511 1545 2229 Fragments arranged by size: 2229 1545 1334 511 97 PasI CC'CwG_GG Cuts at: 1348 2049 1348 Size: 701 5015 PciI A'CATG_T Cuts at: 2559 4615 2559 Size: 2056 3660 PflMI CCAn_nnn'nTGG Cuts at: 1108 2785 1108 Size: 1677 4039 PmeI GTTT'AAAC Cuts at: 3020 3020 Size: 5716 PmlI CAC'GTG Cuts at: 2466 2466 Size: 5716 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3785 3817 5298 5330 3785 Size: 32 1481 32 4171 Fragments arranged by size: 4171 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5716 PsiI TTA'TAA Cuts at: 3286 3286 Size: 5716 PspOMI G'GGCC_C Cuts at: 2113 2113 Size: 5716 PstI C_TGCA'G Cuts at: 830 2658 3068 830 Size: 1828 410 3478 Fragments arranged by size: 3478 1828 410 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5716 PvuII CAG'CTG Cuts at: 2183 2183 Size: 5716 SacI G_AGCT'C Cuts at: 2613 2613 Size: 5716 SalI G'TCGA_C Cuts at: 1936 3058 1936 Size: 1122 4594 SbfI CC_TGCA'GG Cuts at: 3068 3068 Size: 5716 ScaI AGT'ACT Cuts at: 2783 3964 5676 2783 Size: 1181 1712 2823 Fragments arranged by size: 2823 1712 1181 SfcI C'TryA_G Cuts at: 826 1045 2574 2654 3064 4199 4880 5071 826 Size: 219 1529 80 410 1135 681 191 1471 Fragments arranged by size: 1529 1471 1135 681 410 219 191 80 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5716 SmaI CCC'GGG Cuts at: 1872 2137 3043 1872 Size: 265 906 4545 Fragments arranged by size: 4545 906 265 SmlI C'TyrA_G Cuts at: 820 1017 1943 3820 4721 4983 820 Size: 197 926 1877 901 262 1553 Fragments arranged by size: 1877 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3507 493 Size: 3014 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5716 SphI G_CATG'C Cuts at: 1132 3074 1132 Size: 1942 3774 SspI AAT'ATT Cuts at: 5 52 3640 5 Size: 47 3588 2081 Fragments arranged by size: 3588 2081 47 StuI AGG'CCT Cuts at: 1517 2700 1517 Size: 1183 4533 StyI C'CwwG_G Cuts at: 513 1065 2004 2397 2535 2631 2850 3155 513 Size: 552 939 393 138 96 219 305 3074 Fragments arranged by size: 3074 939 552 393 305 219 138 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3743 3760 3913 4098 4543 325 Size: 1255 2163 17 153 185 445 1498 Fragments arranged by size: 2163 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2603 2781 3962 Size: 274 80 33 51 1443 626 178 1181 Cuts at: 3962 5674 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1181 626 274 178 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5676 5708 5676 Size: 32 5684 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5716 XbaI T'CTAG_A Cuts at: 2878 3052 2878 Size: 174 5542 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2368 2404 2368 Size: 36 5680 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5716 XmaI C'CCGG_G Cuts at: 1870 2135 3041 1870 Size: 265 906 4545 Fragments arranged by size: 4545 906 265 XmnI GAAnn'nnTTC Cuts at: 3011 3845 3011 Size: 834 4882 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3524 276 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI