Restriction map for
pFN21AE0284
(Linear) (Six-base) MAPSORT of: KIEE0284.seq Check: 1790 from: 1 to: 1011
KIEE0284.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 0 544 1011
Size: 544 467
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 1011
Size: 149 862
AflIII A'CryG_T
Cuts at: 0 554 1011
Size: 554 457
ApaI G_GGCC'C
Cuts at: 0 112 1011
Size: 112 899
AseI AT'TA_AT
Cuts at: 0 818 1011
Size: 818 193
AvaI C'yCGr_G
Cuts at: 0 111 130 1011
Size: 111 19 881
Fragments arranged by size:
881 111 19
BanII G_rGCy'C
Cuts at: 0 112 310 608 1011
Size: 112 198 298 403
Fragments arranged by size:
403 298 198 112
BbsI GAAGACnn'nnnn_
Cuts at: 0 967 1011
Size: 967 44
BbvCI CC'TCA_GC
Cuts at: 0 892 1011
Size: 892 119
Bme1580I G_kGCm'C
Cuts at: 0 112 1011
Size: 112 899
BmrI ACTGGGnnnn_n'
Cuts at: 0 750 1011
Size: 750 261
BmtI G_CTAG'C
Cuts at: 0 241 1011
Size: 241 770
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 363 1011
Size: 363 648
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 682 1011
Size: 275 407 329
Fragments arranged by size:
407 329 275
Bpu10I CC'TnA_GC
Cuts at: 0 892 1011
Size: 892 119
BsaI GGTCTCn'nnnn_
Cuts at: 0 66 1011
Size: 66 945
BsaAI yAC'GTr
Cuts at: 0 461 1011
Size: 461 550
BsaWI w'CCGG_w
Cuts at: 0 336 1011
Size: 336 675
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 27 44 74 526 556 1011
Size: 27 17 30 452 30 455
Fragments arranged by size:
455 452 30 30 27 17
BseYI C'CCAG_C
Cuts at: 0 174 303 1011
Size: 174 129 708
Fragments arranged by size:
708 174 129
BsiHKAI G_wGCw'C
Cuts at: 0 608 1011
Size: 608 403
Bsp1286I G_dGCh'C
Cuts at: 0 112 310 608 1011
Size: 112 198 298 403
Fragments arranged by size:
403 298 198 112
BspEI T'CCGG_A
Cuts at: 0 336 1011
Size: 336 675
BspHI T'CATG_A
Cuts at: 0 956 1011
Size: 956 55
BsrDI GCAATG_nn'
Cuts at: 0 407 944 1011
Size: 407 537 67
Fragments arranged by size:
537 407 67
BsrFI r'CCGG_y
Cuts at: 0 181 911 1011
Size: 181 730 100
Fragments arranged by size:
730 181 100
BsrGI T'GTAC_A
Cuts at: 0 598 1011
Size: 598 413
BstAPI GCAn_nnn'nTGC
Cuts at: 0 657 1011
Size: 657 354
BstBI TT'CG_AA
Cuts at: 0 197 1011
Size: 197 814
BstXI CCAn_nnnn'nTGG
Cuts at: 0 366 382 1011
Size: 366 16 629
Fragments arranged by size:
629 366 16
BstYI r'GATC_y
Cuts at: 0 702 1011
Size: 702 309
Bsu36I CC'TnA_GG
Cuts at: 0 485 1011
Size: 485 526
BtgI C'CryG_G
Cuts at: 0 375 392 530 626 845 1011
Size: 375 17 138 96 219 166
Fragments arranged by size:
375 219 166 138 96 17
BtsI GCAGTG_nn'
Cuts at: 0 817 1011
Size: 817 194
DrdI GACnn_nn'nnGTC
Cuts at: 0 751 1011
Size: 751 260
EaeI y'GGCC_r
Cuts at: 0 522 1011
Size: 522 489
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 811 1011
Size: 811 200
EcoICRI GAG'CTC
Cuts at: 0 606 1011
Size: 606 405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 275 682 1011
Size: 149 126 407 329
Fragments arranged by size:
407 329 149 126
EcoO109I rG'GnC_Cy
Cuts at: 0 888 1011
Size: 888 123
FseI GG_CCGG'CC
Cuts at: 0 915 1011
Size: 915 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 44 76 468 500 526 558 968
Size: 29 15 32 392 32 26 32 410
Cuts at: 968 1000 1011
Size: 32 11
Fragments arranged by size:
410 392 32 32 32 32 29 26
15 11
MslI CAynn'nnrTG
Cuts at: 0 557 1011
Size: 557 454
MspA1I CmG'CkG
Cuts at: 0 19 178 923 1011
Size: 19 159 745 88
Fragments arranged by size:
745 159 88 19
NaeI GCC'GGC
Cuts at: 0 913 1011
Size: 913 98
NcoI C'CATG_G
Cuts at: 0 392 530 626 845 1011
Size: 392 138 96 219 166
Fragments arranged by size:
392 219 166 138 96
NgoMIV G'CCGG_C
Cuts at: 0 911 1011
Size: 911 100
NheI G'CTAG_C
Cuts at: 0 237 1011
Size: 237 774
NspI r_CATG'y
Cuts at: 0 558 1011
Size: 558 453
PasI CC'CwG_GG
Cuts at: 0 44 1011
Size: 44 967
PciI A'CATG_T
Cuts at: 0 554 1011
Size: 554 457
PflMI CCAn_nnn'nTGG
Cuts at: 0 780 1011
Size: 780 231
PmlI CAC'GTG
Cuts at: 0 461 1011
Size: 461 550
PspOMI G'GGCC_C
Cuts at: 0 108 1011
Size: 108 903
PstI C_TGCA'G
Cuts at: 0 653 1011
Size: 653 358
PvuII CAG'CTG
Cuts at: 0 178 1011
Size: 178 833
SacI G_AGCT'C
Cuts at: 0 608 1011
Size: 608 403
ScaI AGT'ACT
Cuts at: 0 778 1011
Size: 778 233
SfcI C'TryA_G
Cuts at: 0 569 649 1011
Size: 569 80 362
Fragments arranged by size:
569 362 80
SmaI CCC'GGG
Cuts at: 0 132 1011
Size: 132 879
StuI AGG'CCT
Cuts at: 0 695 1011
Size: 695 316
StyI C'CwwG_G
Cuts at: 0 392 530 626 845 1011
Size: 392 138 96 219 166
Fragments arranged by size:
392 219 166 138 96
TatI w'GTAC_w
Cuts at: 0 598 776 1011
Size: 598 178 235
Fragments arranged by size:
598 235 178
XbaI T'CTAG_A
Cuts at: 0 873 1011
Size: 873 138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 363 399 1011
Size: 363 36 612
Fragments arranged by size:
612 363 36
XmaI C'CCGG_G
Cuts at: 0 130 1011
Size: 130 881
XmnI GAAnn'nnTTC
Cuts at: 0 1006 1011
Size: 1006 5
Enzymes that do cut:
AccI AcuI AflIII ApaI AseI AvaI BanII BbsI
BbvCI Bme1580I BmrI BmtI BplI BpmI Bpu10I BsaI
BsaAI BsaWI BsaXI BseYI BsiHKAI Bsp1286I BspEI BspHI
BsrDI BsrFI BsrGI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtsI DrdI EaeI EciI EcoICRI Eco57MI EcoO109I
FseI Hin4I MslI MspA1I NaeI NcoI NgoMIV NheI
NspI PasI PciI PflMI PmlI PspOMI PstI PvuII
SacI ScaI SfcI SmaI StuI StyI TatI XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AsiSI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BpuEI BsaBI BsaHI
BseRI BsgI BsiEI BsiWI BsmI BsmBI BspMI BsrBI
BssHII BssSI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII EagI EarI EcoNI EcoRI EcoRV FalI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NarI NdeI NotI NruI NsiI
PacI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TstI Tth111I XhoI
ZraI