(Circular) (Six-base) MAPSORT of: pFN21AE0281.seq Check: 8637 from: 1 to: 5407 pFN21AE0281 5407 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2159 2375 2552 2159 Size: 216 177 5014 Fragments arranged by size: 5014 216 177 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3217 278 Size: 53 83 186 936 1681 2468 Fragments arranged by size: 2468 1681 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2750 1937 Size: 813 4594 Acc65I G'GTAC_C Cuts at: 2728 2728 Size: 5407 AclI AA'CG_TT Cuts at: 1563 3534 3907 1563 Size: 1971 373 3063 Fragments arranged by size: 3063 1971 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2284 3470 4854 5122 1632 Size: 652 1186 1384 268 1917 Fragments arranged by size: 1917 1384 1186 652 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5210 AflIII A'CryG_T Cuts at: 1203 4306 5283 1203 Size: 3103 977 1327 Fragments arranged by size: 3103 1327 977 AgeI A'CCGG_T Cuts at: 4238 4238 Size: 5407 AhdI GACnn_n'nnGTC Cuts at: 4136 4136 Size: 5407 AleI CACnn'nnGTG Cuts at: 1166 2404 1166 Size: 1238 4169 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2228 2560 1184 Size: 1044 332 4031 Fragments arranged by size: 4031 1044 332 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2129 2161 2129 Size: 32 5375 AlwNI CAG_nnn'CTG Cuts at: 3150 4722 3150 Size: 1572 3835 ApaLI G'TGCA_C Cuts at: 3464 4620 3464 Size: 1156 4251 ApoI r'AATT_y Cuts at: 1493 2716 2942 3094 5203 1493 Size: 1223 226 152 2109 1697 Fragments arranged by size: 2109 1697 1223 226 152 AseI AT'TA_AT Cuts at: 160 1032 3961 160 Size: 872 2929 1606 Fragments arranged by size: 2929 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 AvaI C'yCGr_G Cuts at: 1870 1943 2732 1870 Size: 73 789 4545 Fragments arranged by size: 4545 789 73 BamHI G'GATC_C Cuts at: 2737 3104 2737 Size: 367 5040 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2637 2728 4183 618 Size: 325 217 627 224 626 91 1455 1842 Fragments arranged by size: 1842 1455 627 626 325 224 217 91 BanII G_rGCy'C Cuts at: 1391 2450 2609 2726 1391 Size: 1059 159 117 4072 Fragments arranged by size: 4072 1059 159 117 BbeI G_GCGC'C Cuts at: 2015 2641 2015 Size: 626 4781 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4436 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3598 3632 1903 Size: 34 50 34 1577 34 3678 Fragments arranged by size: 3678 1577 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3299 4509 3299 Size: 1210 4197 BclI T'GATC_A Cuts at: 1548 1548 Size: 5407 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2100 2187 Size: 107 122 71 631 605 337 91 87 Cuts at: 2187 2202 2376 2694 4018 136 Size: 15 174 318 1324 1525 Fragments arranged by size: 1525 1324 631 605 337 318 174 122 107 91 87 71 15 BglII A'GATC_T Cuts at: 5402 5402 Size: 5407 BlpI GC'TnA_GC Cuts at: 2824 2824 Size: 5407 Bme1580I G_kGCm'C Cuts at: 1790 2090 2381 3468 4624 1790 Size: 300 291 1087 1156 2573 Fragments arranged by size: 2573 1156 1087 300 291 BmgBI CAC'GTC Cuts at: 1318 2677 1318 Size: 1359 4048 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2435 3138 4096 Size: 416 53 83 399 24 1018 703 958 Cuts at: 4096 442 Size: 1753 Fragments arranged by size: 1753 1018 958 703 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5407 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2464 2513 4067 1239 Size: 1225 49 1554 2579 Fragments arranged by size: 2579 1554 1225 49 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3532 4397 4695 3532 Size: 865 298 4244 Fragments arranged by size: 4244 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4070 882 Size: 617 2571 2219 Fragments arranged by size: 2571 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3198 493 Size: 711 1994 2702 Fragments arranged by size: 2702 1994 711 BsaBI GATnn'nnATC Cuts at: 5401 5401 Size: 5407 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2638 3214 3596 Size: 53 83 186 936 479 626 576 382 Cuts at: 3596 4231 275 Size: 635 1451 Fragments arranged by size: 1451 936 635 626 576 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2319 3840 4238 4512 4659 5070 2319 Size: 1521 398 274 147 411 2656 Fragments arranged by size: 2656 1521 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5182 Fragments arranged by size: 5182 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2147 2546 2617 1186 Size: 961 399 71 3976 Fragments arranged by size: 3976 961 399 71 BseYI C'CCAG_C Cuts at: 2522 4610 5106 2522 Size: 2088 496 2823 Fragments arranged by size: 2823 2088 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2092 2198 2437 1163 Size: 573 356 106 239 4133 Fragments arranged by size: 4133 573 356 239 106 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2857 3618 4646 1540 Size: 275 186 856 761 1028 2301 Fragments arranged by size: 2301 1028 856 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3468 3553 4624 3468 Size: 85 1071 4251 Fragments arranged by size: 4251 1071 85 BsmI GAATG_Cn' Cuts at: 2918 3011 2918 Size: 93 5314 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2381 2450 2609 2726 3468 3553 Size: 399 300 291 69 159 117 742 85 Cuts at: 3553 4624 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 300 291 159 117 85 69 BspEI T'CCGG_A Cuts at: 5070 5070 Size: 5407 BspHI T'CATG_A Cuts at: 3296 5026 3296 Size: 1730 3677 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2159 2375 2480 2552 2762 844 Size: 1315 216 105 72 210 3489 Fragments arranged by size: 3489 1315 216 210 105 72 BsrBI CCG'CTC Cuts at: 1394 1823 3294 1394 Size: 429 1471 3507 Fragments arranged by size: 3507 1471 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3902 4076 65 Size: 1183 2654 174 1396 Fragments arranged by size: 2654 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2634 4051 4238 1700 Size: 110 824 1417 187 2869 Fragments arranged by size: 2869 1417 824 187 110 BsrGI T'GTAC_A Cuts at: 96 2343 96 Size: 2247 3160 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5407 BssSI C'ACGA_G Cuts at: 3467 4479 3467 Size: 1012 4395 BstAPI GCAn_nnn'nTGC Cuts at: 794 2322 794 Size: 1528 3879 BstBI TT'CG_AA Cuts at: 4211 4211 Size: 5407 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5407 BstYI r'GATC_y Cuts at: 1972 2737 3104 3489 3506 4958 5044 5056 5402 Size: 765 367 385 17 1452 86 12 346 Cuts at: 5402 1972 Size: 1977 Fragments arranged by size: 1977 1452 765 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2046 2245 513 Size: 552 981 199 3675 Fragments arranged by size: 3675 981 552 199 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4668 Fragments arranged by size: 4668 683 56 BtsI GCAGTG_nn' Cuts at: 2923 3717 3737 2923 Size: 794 20 4593 Fragments arranged by size: 4593 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5407 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2257 2292 529 Size: 35 1693 35 3644 Fragments arranged by size: 3644 1693 35 35 DraI TTT'AAA Cuts at: 2711 2711 Size: 5407 DraIII CAC_nnn'GTG Cuts at: 2047 2047 Size: 5407 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4414 809 Size: 507 3098 1802 Fragments arranged by size: 3098 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2248 2515 2611 Size: 54 1425 50 275 18 418 267 96 Cuts at: 2611 2854 3743 5372 8 Size: 243 889 1629 43 Fragments arranged by size: 1629 1425 889 418 275 267 243 96 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2854 1537 Size: 275 1042 4090 Fragments arranged by size: 4090 1042 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2171 3337 1353 Size: 818 1166 3423 Fragments arranged by size: 3423 1166 818 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2168 2183 3994 4368 4514 387 Size: 1064 138 579 15 1811 374 146 1280 Fragments arranged by size: 1811 1280 1064 579 374 146 138 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2284 2464 2513 3470 4067 4854 5122 Size: 393 652 180 49 957 597 787 268 Cuts at: 5122 1239 Size: 1524 Fragments arranged by size: 1524 957 787 652 597 393 268 180 49 EcoO109I rG'GnC_Cy Cuts at: 2429 2488 2586 2429 Size: 59 98 5250 Fragments arranged by size: 5250 98 59 EcoRI G'AATT_C Cuts at: 2716 3094 2716 Size: 378 5029 FspI TGC'GCA Cuts at: 3913 3913 Size: 5407 HaeII r_GCGC'y Cuts at: 1678 2015 2641 4554 1678 Size: 337 626 1913 2531 Fragments arranged by size: 2531 1913 626 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4048 4080 4122 4154 1373 Size: 32 135 32 2476 32 42 32 2626 Fragments arranged by size: 2626 2476 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2260 2751 2997 669 Size: 988 281 322 491 246 3079 Fragments arranged by size: 3079 988 491 322 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5407 HpaI GTT'AAC Cuts at: 2997 2997 Size: 5407 KasI G'GCGC_C Cuts at: 2011 2637 2011 Size: 626 4781 KpnI G_GTAC'C Cuts at: 2732 2732 Size: 5407 MfeI C'AATT_G Cuts at: 3006 3006 Size: 5407 MluI A'CGCG_T Cuts at: 5283 5283 Size: 5407 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4521 4705 1305 Size: 3216 184 2007 Fragments arranged by size: 3216 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3585 Fragments arranged by size: 3585 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2404 3365 3724 3883 518 Size: 648 1238 961 359 159 2042 Fragments arranged by size: 2042 1238 961 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2247 2823 3500 4648 4893 1606 Size: 641 576 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 641 576 245 NarI GG'CG_CC Cuts at: 2012 2638 2012 Size: 626 4781 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4855 NdeI CA'TA_TG Cuts at: 387 387 Size: 5407 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5407 NotI GC'GGCC_GC Cuts at: 2854 2854 Size: 5407 NspI r_CATG'y Cuts at: 1132 1229 2056 2765 4310 1132 Size: 97 827 709 1545 2229 Fragments arranged by size: 2229 1545 827 709 97 PasI CC'CwG_GG Cuts at: 1348 2228 2433 1348 Size: 880 205 4322 Fragments arranged by size: 4322 880 205 PciI A'CATG_T Cuts at: 4306 4306 Size: 5407 PflMI CCAn_nnn'nTGG Cuts at: 1108 2047 1108 Size: 939 4468 PmeI GTTT'AAAC Cuts at: 2711 2711 Size: 5407 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3476 3508 4989 5021 3476 Size: 32 1481 32 3862 Fragments arranged by size: 3862 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2429 2429 Size: 5407 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5407 PsiI TTA'TAA Cuts at: 2977 2977 Size: 5407 PstI C_TGCA'G Cuts at: 830 2703 2759 830 Size: 1873 56 3478 Fragments arranged by size: 3478 1873 56 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5407 SacII CC_GC'GG Cuts at: 2248 2248 Size: 5407 SalI G'TCGA_C Cuts at: 1936 2749 1936 Size: 813 4594 SanDI GG'GwC_CC Cuts at: 2429 2429 Size: 5407 SapI GCTCTTCn'nnn_ Cuts at: 2171 2171 Size: 5407 SbfI CC_TGCA'GG Cuts at: 2759 2759 Size: 5407 ScaI AGT'ACT Cuts at: 3655 5367 3655 Size: 1712 3695 SfcI C'TryA_G Cuts at: 826 1045 2699 2755 3890 4571 4762 826 Size: 219 1654 56 1135 681 191 1471 Fragments arranged by size: 1654 1471 1135 681 219 191 56 SfoI GGC'GCC Cuts at: 2013 2639 2013 Size: 626 4781 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5407 SmaI CCC'GGG Cuts at: 1872 2734 1872 Size: 862 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3511 4412 4674 820 Size: 197 926 1568 901 262 1553 Fragments arranged by size: 1568 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3198 493 Size: 2705 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5407 SphI G_CATG'C Cuts at: 1132 2765 1132 Size: 1633 3774 SspI AAT'ATT Cuts at: 5 52 3331 5 Size: 47 3279 2081 Fragments arranged by size: 3279 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5407 StyI C'CwwG_G Cuts at: 513 1065 2846 513 Size: 552 1781 3074 Fragments arranged by size: 3074 1781 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3434 3451 3604 3789 4234 325 Size: 1255 1854 17 153 185 445 1498 Fragments arranged by size: 1854 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2343 3653 5365 Size: 274 80 33 51 1443 366 1310 1712 Cuts at: 5365 96 Size: 138 Fragments arranged by size: 1712 1443 1310 366 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5367 5399 5367 Size: 32 5375 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5407 XbaI T'CTAG_A Cuts at: 2743 2743 Size: 5407 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5407 XmaI C'CCGG_G Cuts at: 1870 2732 1870 Size: 862 4545 XmnI GAAnn'nnTTC Cuts at: 3536 3536 Size: 5407 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3215 276 Size: 53 83 186 936 1681 2468 Fragments arranged by size: 2468 1681 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SexAI SfiI SrfI SwaI XcmI