Restriction map for
pFN21AE0275
(Linear) (Six-base) MAPSORT of: KIEE0275.seq Check: 5211 from: 1 to: 522
KIEE0275.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 231 522
Size: 231 291
AfeI AGC'GCT
Cuts at: 0 299 522
Size: 299 223
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 457 522
Size: 457 65
BanII G_rGCy'C
Cuts at: 0 53 416 522
Size: 53 363 106
Fragments arranged by size:
363 106 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 25 522
Size: 25 497
BglI GCCn_nnn'nGGC
Cuts at: 0 395 463 522
Size: 395 68 59
Fragments arranged by size:
395 68 59
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 213 522
Size: 213 309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 385 522
Size: 148 237 137
Fragments arranged by size:
237 148 137
Bpu10I CC'TnA_GC
Cuts at: 0 294 522
Size: 294 228
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 340 370 522
Size: 340 30 152
Fragments arranged by size:
340 152 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 166 187 364 406 522
Size: 166 21 177 42 116
Fragments arranged by size:
177 166 116 42 21
BseYI C'CCAG_C
Cuts at: 0 236 303 453 511 522
Size: 236 67 150 58 11
Fragments arranged by size:
236 150 67 58 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 522
Size: 427 95
BsiEI CG_ry'CG
Cuts at: 0 382 522
Size: 382 140
BsiHKAI G_wGCw'C
Cuts at: 0 53 416 522
Size: 53 363 106
Fragments arranged by size:
363 106 53
Bsp1286I G_dGCh'C
Cuts at: 0 53 416 522
Size: 53 363 106
Fragments arranged by size:
363 106 53
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 231 522
Size: 231 291
BsrBI CCG'CTC
Cuts at: 0 192 522
Size: 192 330
BssHII G'CGCG_C
Cuts at: 0 258 522
Size: 258 264
BstAPI GCAn_nnn'nTGC
Cuts at: 0 431 522
Size: 431 91
BstYI r'GATC_y
Cuts at: 0 368 489 522
Size: 368 121 33
Fragments arranged by size:
368 121 33
BtgI C'CryG_G
Cuts at: 0 160 390 522
Size: 160 230 132
Fragments arranged by size:
230 160 132
BtsI GCAGTG_nn'
Cuts at: 0 468 522
Size: 468 54
EarI CTCTTCn'nnn_
Cuts at: 0 41 424 522
Size: 41 383 98
Fragments arranged by size:
383 98 41
EcoICRI GAG'CTC
Cuts at: 0 51 414 522
Size: 51 363 108
Fragments arranged by size:
363 108 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 385 522
Size: 148 237 137
Fragments arranged by size:
237 148 137
EcoNI CCTnn'n_nnAGG
Cuts at: 0 346 522
Size: 346 176
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 42 522
Size: 42 480
HaeII r_GCGC'y
Cuts at: 0 198 250 301 522
Size: 198 52 51 221
Fragments arranged by size:
221 198 52 51
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 522
Size: 74 448
MspA1I CmG'CkG
Cuts at: 0 126 162 216 269 392 511 522
Size: 126 36 54 53 123 119 11
Fragments arranged by size:
126 123 119 54 53 36 11
PflMI CCAn_nnn'nTGG
Cuts at: 0 243 522
Size: 243 279
PfoI T'CCnGG_A
Cuts at: 0 344 522
Size: 344 178
SacI G_AGCT'C
Cuts at: 0 53 416 522
Size: 53 363 106
Fragments arranged by size:
363 106 53
SacII CC_GC'GG
Cuts at: 0 163 393 522
Size: 163 230 129
Fragments arranged by size:
230 163 129
SapI GCTCTTCn'nnn_
Cuts at: 0 41 522
Size: 41 481
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 250 467 522
Size: 250 217 55
Fragments arranged by size:
250 217 55
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 340 372 522
Size: 340 32 150
Fragments arranged by size:
340 150 32
Enzymes that do cut:
AarI AfeI AlfI BanII BbsI BglI BplI BpmI
Bpu10I BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BssHII BstAPI BstYI BtgI BtsI EarI
EcoICRI Eco57MI EcoNI FalI HaeII MmeI MspA1I PflMI
PfoI SacI SacII SapI TaqII TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI
BsrGI BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoO109I EcoRI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TatI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI