(Circular) (Six-base) MAPSORT of: pFN21AE0269.seq Check: 3424 from: 1 to: 6004 pFN21AE0269 6004 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3060 3060 Size: 6004 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3814 278 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3347 1937 Size: 1410 4594 Acc65I G'GTAC_C Cuts at: 3325 3325 Size: 6004 AclI AA'CG_TT Cuts at: 1563 4131 4504 1563 Size: 2568 373 3063 Fragments arranged by size: 3063 2568 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4067 5451 5719 1632 Size: 2435 1384 268 1917 Fragments arranged by size: 2435 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5807 AflIII A'CryG_T Cuts at: 1203 4903 5880 1203 Size: 3700 977 1327 Fragments arranged by size: 3700 1327 977 AgeI A'CCGG_T Cuts at: 4835 4835 Size: 6004 AhdI GACnn_n'nnGTC Cuts at: 4733 4733 Size: 6004 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6004 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2522 2951 1184 Size: 1338 429 4237 Fragments arranged by size: 4237 1338 429 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2127 2159 2127 Size: 32 5972 AlwNI CAG_nnn'CTG Cuts at: 2138 2519 2543 3022 3221 3747 5319 2138 Size: 381 24 479 199 526 1572 2823 Fragments arranged by size: 2823 1572 526 479 381 199 24 ApaI G_GGCC'C Cuts at: 3185 3185 Size: 6004 ApaLI G'TGCA_C Cuts at: 4061 5217 4061 Size: 1156 4848 ApoI r'AATT_y Cuts at: 1493 2026 2785 3313 3539 3691 5800 1493 Size: 533 759 528 226 152 2109 1697 Fragments arranged by size: 2109 1697 759 533 528 226 152 AseI AT'TA_AT Cuts at: 160 1032 4558 160 Size: 872 3526 1606 Fragments arranged by size: 3526 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 AvaI C'yCGr_G Cuts at: 1870 1943 3087 3286 3329 1870 Size: 73 1144 199 43 4545 Fragments arranged by size: 4545 1144 199 73 43 AvrII C'CTAG_G Cuts at: 3173 3173 Size: 6004 BamHI G'GATC_C Cuts at: 3107 3334 3701 3107 Size: 227 367 5410 Fragments arranged by size: 5410 367 227 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2873 3048 3325 4780 618 Size: 325 217 627 1086 175 277 1455 1842 Fragments arranged by size: 1842 1455 1086 627 325 277 217 175 BanII G_rGCy'C Cuts at: 1391 3185 3323 1391 Size: 1794 138 4072 Fragments arranged by size: 4072 1794 138 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3298 928 Size: 971 1399 3634 Fragments arranged by size: 3634 1399 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4195 4229 1903 Size: 34 50 34 2174 34 3678 Fragments arranged by size: 3678 2174 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3896 5106 3896 Size: 1210 4794 BclI T'GATC_A Cuts at: 1548 1548 Size: 6004 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4615 136 Size: 107 122 71 631 605 2943 1525 Fragments arranged by size: 2943 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5999 5999 Size: 6004 BlpI GC'TnA_GC Cuts at: 3421 3421 Size: 6004 Bme1580I G_kGCm'C Cuts at: 1790 2852 3185 4065 5221 1790 Size: 1062 333 880 1156 2573 Fragments arranged by size: 2573 1156 1062 880 333 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6004 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2529 2627 3735 Size: 416 53 83 399 24 1112 98 1108 Cuts at: 3735 4693 442 Size: 958 1753 Fragments arranged by size: 1753 1112 1108 958 416 399 98 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6004 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2164 2519 2164 Size: 355 5649 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2975 3032 4664 1239 Size: 1736 57 1632 2579 Fragments arranged by size: 2579 1736 1632 57 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4129 4994 5292 4129 Size: 865 298 4841 Fragments arranged by size: 4841 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3138 4667 882 Size: 617 1639 1529 2219 Fragments arranged by size: 2219 1639 1529 617 BsaAI yAC'GTr Cuts at: 493 1204 3795 493 Size: 711 2591 2702 Fragments arranged by size: 2702 2591 711 BsaBI GATnn'nnATC Cuts at: 2283 5998 2283 Size: 3715 2289 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3811 4193 4828 275 Size: 53 83 186 936 2278 382 635 1451 Fragments arranged by size: 2278 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4437 4835 5109 5256 5667 4437 Size: 398 274 147 411 4774 Fragments arranged by size: 4774 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2100 2130 1178 Size: 30 165 30 697 30 5052 Fragments arranged by size: 5052 697 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2309 1186 Size: 1123 4881 BseYI C'CCAG_C Cuts at: 2894 3036 3178 5207 5703 2894 Size: 142 142 2029 496 3195 Fragments arranged by size: 3195 2029 496 142 142 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2852 1163 Size: 573 1116 4315 Fragments arranged by size: 4315 1116 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3454 4215 5243 1540 Size: 275 186 1453 761 1028 2301 Fragments arranged by size: 2301 1453 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2998 3168 4065 4150 5221 2998 Size: 170 897 85 1071 3781 Fragments arranged by size: 3781 1071 897 170 85 BsmI GAATG_Cn' Cuts at: 3515 3608 3515 Size: 93 5911 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2852 2998 3168 3185 3323 4065 4150 Size: 399 1062 146 170 17 138 742 85 Cuts at: 4150 5221 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1062 742 399 170 146 138 85 17 BspEI T'CCGG_A Cuts at: 5667 5667 Size: 6004 BspHI T'CATG_A Cuts at: 2454 3893 5623 2454 Size: 1439 1730 2835 Fragments arranged by size: 2835 1730 1439 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3060 3359 844 Size: 2216 299 3489 Fragments arranged by size: 3489 2216 299 BsrBI CCG'CTC Cuts at: 1394 1823 3891 1394 Size: 429 2068 3507 Fragments arranged by size: 3507 2068 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4499 4673 65 Size: 1183 3251 174 1396 Fragments arranged by size: 3251 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3027 3045 4648 4835 1700 Size: 110 1217 18 1603 187 2869 Fragments arranged by size: 2869 1603 1217 187 110 18 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6004 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6004 BssSI C'ACGA_G Cuts at: 2153 2396 4064 5076 2153 Size: 243 1668 1012 3081 Fragments arranged by size: 3081 1668 1012 243 BstAPI GCAn_nnn'nTGC Cuts at: 794 2543 794 Size: 1749 4255 BstBI TT'CG_AA Cuts at: 2092 4808 2092 Size: 2716 3288 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6004 BstXI CCAn_nnnn'nTGG Cuts at: 2422 2617 2422 Size: 195 5809 BstYI r'GATC_y Cuts at: 1972 2728 3107 3334 3701 4086 4103 5555 5641 Size: 756 379 227 367 385 17 1452 86 Cuts at: 5641 5653 5999 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 756 385 379 367 346 227 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5452 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2269 487 Size: 683 56 1043 4222 Fragments arranged by size: 4222 1043 683 56 BtsI GCAGTG_nn' Cuts at: 3520 4314 4334 3520 Size: 794 20 5190 Fragments arranged by size: 5190 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6004 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5969 DraI TTT'AAA Cuts at: 3308 3308 Size: 6004 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5011 809 Size: 507 3695 1802 Fragments arranged by size: 3695 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2424 2748 2793 Size: 54 1425 50 275 18 594 324 45 Cuts at: 2793 2859 3238 3451 4340 5969 8 Size: 66 379 213 889 1629 43 Fragments arranged by size: 1629 1425 889 594 379 324 275 213 66 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3451 1537 Size: 275 1639 4090 Fragments arranged by size: 4090 1639 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2238 2437 3934 1353 Size: 885 199 1497 3423 Fragments arranged by size: 3423 1497 885 199 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4591 4965 5111 387 Size: 1064 138 3002 374 146 1280 Fragments arranged by size: 3002 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2975 3032 4067 4664 5451 5719 1239 Size: 393 1343 57 1035 597 787 268 1524 Fragments arranged by size: 1524 1343 1035 787 597 393 268 57 EcoO109I rG'GnC_Cy Cuts at: 2621 3182 3283 2621 Size: 561 101 5342 Fragments arranged by size: 5342 561 101 EcoRI G'AATT_C Cuts at: 3313 3691 3313 Size: 378 5626 FspI TGC'GCA Cuts at: 4510 4510 Size: 6004 HaeII r_GCGC'y Cuts at: 1678 5151 1678 Size: 3473 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2100 2132 4645 4677 4719 Size: 32 135 32 528 32 2513 32 42 Cuts at: 4719 4751 1373 Size: 32 2626 Fragments arranged by size: 2626 2513 528 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2196 2386 3348 3594 669 Size: 988 281 258 190 962 246 3079 Fragments arranged by size: 3079 988 962 281 258 246 190 HindIII A'AGCT_T Cuts at: 748 748 Size: 6004 HpaI GTT'AAC Cuts at: 3594 3594 Size: 6004 KpnI G_GTAC'C Cuts at: 3329 3329 Size: 6004 MfeI C'AATT_G Cuts at: 3603 3603 Size: 6004 MluI A'CGCG_T Cuts at: 5880 5880 Size: 6004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2153 2781 5118 5302 1305 Size: 848 628 2337 184 2007 Fragments arranged by size: 2337 2007 848 628 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2426 2750 2795 2861 3240 Size: 54 1425 343 594 324 45 66 379 Cuts at: 3240 10 Size: 2774 Fragments arranged by size: 2774 1425 594 379 343 324 66 54 45 MslI CAynn'nnrTG Cuts at: 518 1166 2208 2774 3962 4321 4480 518 Size: 648 1042 566 1188 359 159 2042 Fragments arranged by size: 2042 1188 1042 648 566 359 159 MspA1I CmG'CkG Cuts at: 1606 2543 3420 4097 5245 5490 1606 Size: 937 877 677 1148 245 2120 Fragments arranged by size: 2120 1148 937 877 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5452 NdeI CA'TA_TG Cuts at: 387 387 Size: 6004 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6004 NotI GC'GGCC_GC Cuts at: 3451 3451 Size: 6004 NspI r_CATG'y Cuts at: 1132 1229 3362 4907 1132 Size: 97 2133 1545 2229 Fragments arranged by size: 2229 2133 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6004 PciI A'CATG_T Cuts at: 4903 4903 Size: 6004 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6004 PmeI GTTT'AAAC Cuts at: 3308 3308 Size: 6004 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4073 4105 5586 5618 4073 Size: 32 1481 32 4459 Fragments arranged by size: 4459 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6004 PsiI TTA'TAA Cuts at: 3574 3574 Size: 6004 PspOMI G'GGCC_C Cuts at: 3181 3181 Size: 6004 PstI C_TGCA'G Cuts at: 830 2940 3356 830 Size: 2110 416 3478 Fragments arranged by size: 3478 2110 416 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6004 PvuII CAG'CTG Cuts at: 2543 2543 Size: 6004 SalI G'TCGA_C Cuts at: 1936 3346 1936 Size: 1410 4594 SapI GCTCTTCn'nnn_ Cuts at: 2238 2437 2238 Size: 199 5805 SbfI CC_TGCA'GG Cuts at: 3356 3356 Size: 6004 ScaI AGT'ACT Cuts at: 4252 5964 4252 Size: 1712 4292 SfcI C'TryA_G Cuts at: 826 1045 2936 3352 4487 5168 5359 826 Size: 219 1891 416 1135 681 191 1471 Fragments arranged by size: 1891 1471 1135 681 416 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6004 SmaI CCC'GGG Cuts at: 1872 3089 3288 3331 1872 Size: 1217 199 43 4545 Fragments arranged by size: 4545 1217 199 43 SmlI C'TyrA_G Cuts at: 820 1017 1943 4108 5009 5271 820 Size: 197 926 2165 901 262 1553 Fragments arranged by size: 2165 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3795 493 Size: 3302 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6004 SphI G_CATG'C Cuts at: 1132 3362 1132 Size: 2230 3774 SspI AAT'ATT Cuts at: 5 52 3928 5 Size: 47 3876 2081 Fragments arranged by size: 3876 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6004 StyI C'CwwG_G Cuts at: 513 1065 2046 3173 3227 3443 513 Size: 552 981 1127 54 216 3074 Fragments arranged by size: 3074 1127 981 552 216 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2869 2876 3050 4031 4048 4201 4386 Size: 1255 1289 7 174 981 17 153 185 Cuts at: 4386 4831 325 Size: 445 1498 Fragments arranged by size: 1498 1289 1255 981 445 185 174 153 17 7 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4250 5962 96 Size: 274 80 33 51 1443 2273 1712 138 Fragments arranged by size: 2273 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3099 3131 5964 5996 3099 Size: 32 2833 32 3107 Fragments arranged by size: 3107 2833 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6004 XbaI T'CTAG_A Cuts at: 3340 3340 Size: 6004 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2416 2416 Size: 6004 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6004 XmaI C'CCGG_G Cuts at: 1870 3087 3286 3329 1870 Size: 1217 199 43 4545 Fragments arranged by size: 4545 1217 199 43 XmnI GAAnn'nnTTC Cuts at: 2587 4133 2587 Size: 1546 4458 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3812 276 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI