Restriction map for
pFN21AE0268
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BcgI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsgI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstEII BstF5I BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DsaI Eam1105I Eco47III EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI HindIII HinfI HpaI
HphI KpnI KspI MaeI MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacII SbfI ScaI
SexAI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 625 GTCGAC GT^[AC][GT]AC
AccII 123 CGCG CG^CG
AlwI 588 GATCC GGATC(4/5)
AocI 37 CCTAAGG CC^T[ACGT]AGG
AvaI 229 CCCGAG C^[CT]CG[GA]G
BbeI 26 GGCGCC GGCGC^C
BbsI 400 GAAGAC GAAGAC(2/6)
BbvCI 564 CCTCAGC CCTCAGC(-5/-2)
BglI 538 GCCCTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 362 AGATCT A^GATCT
BpuAI 400 GAAGAC GAAGAC(2/6)
BsaI 275 GAGACC GGTCTC(1/5)
BseRI 232 GAGGAG GAGGAG(10/8)
BsmBI 439 GAGACG CGTCTC(1/5)
BsoBI 229 CCCGAG C^[CT]CG[GA]G
BspMI 249 GCAGGT ACCTGC(4/8)
BsrDI 195 CATTGC GCAATG(2/0)
BstBI 631 TTCGAA TT^CGAA
BstUI 123 CGCG CG^CG
Bsu36I 37 CCTAAGG CC^T[ACGT]AGG
DrdI 616 GACGGTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 507 CGGCCG [CT]^GGCC[GA]
EagI 507 CGGCCG C^GGCCG
EclXI 507 CGGCCG C^GGCCG
Eco52I 507 CGGCCG C^GGCCG
Eco81I 37 CCTAAGG CC^T[ACGT]AGG
HincII 625 GTCGAC GT[CT]^[GA]AC
HindII 625 GTCGAC GT[CT]^[GA]AC
KasI 26 GGCGCC G^GCGCC
MaeII 599 ACGT A^CGT
MspA1I 571 CAGCGG C[AC]G^C[GT]G
MvnI 123 CGCG CG^CG
NarI 26 GGCGCC GG^CGCC
NspV 631 TTCGAA TT^CGAA
PflMI 594 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 247 CTGCAG CTGCA^G
SalI 625 GTCGAC G^TCGAC
SapI 241 GAAGAGC GCTCTTC(1/4)
SfcI 247 CTGCAG C^T[GA][CT]AG
SfiI 537 GGCCCTCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 26 GGCGCC GGC^GCC
SfuI 631 TTCGAA TT^CGAA
SmlI 541 CTCAAG C^T[CT][GA]AG
SphI 317 GCATGC GCATG^C
Van91I 594 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
ApaI 33 GGGCCC GGGCC^C
420 GGGCCC
BanI 26 GGCGCC G^G[CT][GA]CC
310 GGTGCC
BpmI 96 CTCCAG CTGGAG(16/14)
592 CTCCAG
BsiEI 213 CGACCG CG[GA][CT]^CG
507 CGGCCG
BstXI 341 CCACCGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
425 CCAAGCTGCTGG
BstYI 362 AGATCT [GA]^GATC[CT]
587 AGATCC
DdeI 38 CTAAG C^T[ACGT]AG
565 CTCAG
EarI 241 GAAGAG CTCTTC(1/4)
634 GAAGAG
EcoT14I 70 CCAAGG C^C[AT][AT]GG
104 CCAAGG
FauI 121 CCCGC CCCGC(4/6)
573 GCGGG
HaeII 26 GGCGCC [GA]GCGC^[CT]
560 AGCGCC
HgaI 475 GACGC GACGC(5/10)
131 GCGTC
Ksp632I 241 GAAGAG CTCTTC(1/4)
634 GAAGAG
MflI 362 AGATCT [GA]^GATC[CT]
587 AGATCC
MslI 342 CACCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
513 CATCAGCGTG
NspI 317 GCATGC [GA]CATG^[CT]
335 ACATGC
PpuMI 41 AGGACCC [GA]G^G[AT]CC[CT]
116 GGGACCC
SacI 352 GAGCTC GAGCT^C
526 GAGCTC
SfaNI 176 GCATC GCATC(5/9)
512 GCATC
StyI 70 CCAAGG C^C[AT][AT]GG
104 CCAAGG
TspRI 52 CAGTG CA[GC]TG[ACGT][ACGT]^
378 CAGTG
XhoII 362 AGATCT [GA]^GATC[CT]
587 AGATCC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcyI AluI AspHI AvaII
BanII BbvI BcnI BmyI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstNI Cac8I
CfoI Cfr13I DpnII DraII EcoO109I
EcoRII Fnu4HI HaeIII HapII HhaI
Hin1I HinP1I HpaII ItaI MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TaqI TseI TthHB8I