(Circular) (Six-base) MAPSORT of: pFN21AE0268.seq Check: 753 from: 1 to: 5365 pFN21AE0268 5365 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3175 278 Size: 53 83 186 936 1639 2468 Fragments arranged by size: 2468 1639 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2631 2708 1937 Size: 694 77 4594 Fragments arranged by size: 4594 694 77 Acc65I G'GTAC_C Cuts at: 2686 2686 Size: 5365 AclI AA'CG_TT Cuts at: 1563 3492 3865 1563 Size: 1929 373 3063 Fragments arranged by size: 3063 1929 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3428 4812 5080 1632 Size: 1796 1384 268 1917 Fragments arranged by size: 1917 1796 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5168 AflIII A'CryG_T Cuts at: 1203 4264 5241 1203 Size: 3061 977 1327 Fragments arranged by size: 3061 1327 977 AgeI A'CCGG_T Cuts at: 4196 4196 Size: 5365 AhdI GACnn_n'nnGTC Cuts at: 4094 4094 Size: 5365 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5365 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2204 1184 Size: 1020 4345 AlwNI CAG_nnn'CTG Cuts at: 3108 4680 3108 Size: 1572 3793 ApaI G_GGCC'C Cuts at: 2042 2429 2042 Size: 387 4978 ApaLI G'TGCA_C Cuts at: 3422 4578 3422 Size: 1156 4209 ApoI r'AATT_y Cuts at: 1493 2674 2900 3052 5161 1493 Size: 1181 226 152 2109 1697 Fragments arranged by size: 2109 1697 1181 226 152 AseI AT'TA_AT Cuts at: 160 1032 3919 160 Size: 872 2887 1606 Fragments arranged by size: 2887 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5365 AvaI C'yCGr_G Cuts at: 1870 1943 2234 2661 2690 1870 Size: 73 291 427 29 4545 Fragments arranged by size: 4545 427 291 73 29 BamHI G'GATC_C Cuts at: 2695 3062 2695 Size: 367 4998 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2031 2315 2686 4141 618 Size: 325 217 627 244 284 371 1455 1842 Fragments arranged by size: 1842 1455 627 371 325 284 244 217 BanII G_rGCy'C Cuts at: 1391 2042 2084 2361 2429 2535 2684 1391 Size: 651 42 277 68 106 149 4072 Fragments arranged by size: 4072 651 277 149 106 68 42 BbeI G_GCGC'C Cuts at: 2035 2035 Size: 5365 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2412 928 Size: 971 513 3881 Fragments arranged by size: 3881 971 513 BbvCI CC'TCA_GC Cuts at: 2570 2570 Size: 5365 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3556 3590 1903 Size: 34 1619 34 3678 Fragments arranged by size: 3678 1619 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3257 4467 3257 Size: 1210 4155 BclI T'GATC_A Cuts at: 1548 1548 Size: 5365 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2549 3976 136 Size: 107 122 71 631 605 877 1427 1525 Fragments arranged by size: 1525 1427 877 631 605 122 107 71 BglII A'GATC_T Cuts at: 2367 5360 2367 Size: 2993 2372 BlpI GC'TnA_GC Cuts at: 2782 2782 Size: 5365 Bme1580I G_kGCm'C Cuts at: 1790 2042 2429 3426 4582 1790 Size: 252 387 997 1156 2573 Fragments arranged by size: 2573 1156 997 387 252 BmgBI CAC'GTC Cuts at: 1318 2605 1318 Size: 1287 4078 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3096 4054 442 Size: 416 53 83 399 24 1679 958 1753 Fragments arranged by size: 1753 1679 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5365 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2086 2582 4025 1239 Size: 847 496 1443 2579 Fragments arranged by size: 2579 1443 847 496 Bpu10I CC'TnA_GC Cuts at: 2570 2570 Size: 5365 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2531 3490 4355 4653 2531 Size: 959 865 298 3243 Fragments arranged by size: 3243 959 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2274 4028 882 Size: 617 775 1754 2219 Fragments arranged by size: 2219 1754 775 617 BsaAI yAC'GTr Cuts at: 493 1204 3156 493 Size: 711 1952 2702 Fragments arranged by size: 2702 1952 711 BsaBI GATnn'nnATC Cuts at: 5359 5359 Size: 5365 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2032 2136 2481 3172 Size: 53 83 186 936 499 104 345 691 Cuts at: 3172 3554 4189 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 691 635 499 382 345 186 104 83 53 BsaWI w'CCGG_w Cuts at: 3798 4196 4470 4617 5028 3798 Size: 398 274 147 411 4135 Fragments arranged by size: 4135 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5140 Fragments arranged by size: 5140 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2252 1186 Size: 1066 4299 BseYI C'CCAG_C Cuts at: 2090 2096 2195 2311 2557 4568 5064 2090 Size: 6 99 116 246 2011 496 2391 Fragments arranged by size: 2391 2011 496 246 116 99 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4792 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2221 2515 2815 3576 4604 1540 Size: 275 186 220 294 300 761 1028 2301 Fragments arranged by size: 2301 1028 761 300 294 275 220 186 BsiHKAI G_wGCw'C Cuts at: 2157 2361 2535 3426 3511 4582 2157 Size: 204 174 891 85 1071 2940 Fragments arranged by size: 2940 1071 891 204 174 85 BsmI GAATG_Cn' Cuts at: 2876 2969 2876 Size: 93 5272 BsmBI CGTCTCn'nnnn_ Cuts at: 2438 2438 Size: 5365 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2084 2157 2361 2429 2535 2684 Size: 399 252 42 73 204 68 106 149 Cuts at: 2684 3426 3511 4582 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 252 204 149 106 85 73 68 42 BspEI T'CCGG_A Cuts at: 5028 5028 Size: 5365 BspHI T'CATG_A Cuts at: 3254 4984 3254 Size: 1730 3635 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2245 2720 844 Size: 1401 475 3489 Fragments arranged by size: 3489 1401 475 BsrBI CCG'CTC Cuts at: 1394 1823 3252 1394 Size: 429 1429 3507 Fragments arranged by size: 3507 1429 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2199 3860 4034 65 Size: 1183 951 1661 174 1396 Fragments arranged by size: 1661 1396 1183 951 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4009 4196 1700 Size: 110 2199 187 2869 Fragments arranged by size: 2869 2199 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5365 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5365 BssSI C'ACGA_G Cuts at: 3425 4437 3425 Size: 1012 4353 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5365 BstBI TT'CG_AA Cuts at: 2637 4169 2637 Size: 1532 3833 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5365 BstXI CCAn_nnnn'nTGG Cuts at: 2353 2437 2353 Size: 84 5281 BstYI r'GATC_y Cuts at: 1972 2367 2592 2695 3062 3447 3464 4916 5002 Size: 395 225 103 367 385 17 1452 86 Cuts at: 5002 5014 5360 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 395 385 367 346 225 103 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2043 2043 Size: 5365 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3874 Fragments arranged by size: 3874 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4626 Fragments arranged by size: 4626 683 56 BtsI GCAGTG_nn' Cuts at: 2063 2881 3675 3695 2063 Size: 818 794 20 3733 Fragments arranged by size: 3733 818 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5365 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5330 DraI TTT'AAA Cuts at: 2669 2669 Size: 5365 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2627 4372 809 Size: 507 1311 1745 1802 Fragments arranged by size: 1802 1745 1311 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2512 2812 3701 Size: 54 1425 50 275 18 682 300 889 Cuts at: 3701 5330 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 682 300 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2512 2812 1537 Size: 275 700 300 4090 Fragments arranged by size: 4090 700 300 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2241 2634 3295 1353 Size: 888 393 661 3423 Fragments arranged by size: 3423 888 661 393 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2403 3952 4326 4472 387 Size: 1064 138 814 1549 374 146 1280 Fragments arranged by size: 1549 1280 1064 814 374 146 138 EcoICRI GAG'CTC Cuts at: 2359 2533 2359 Size: 174 5191 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2086 2582 3428 4025 4812 5080 1239 Size: 393 454 496 846 597 787 268 1524 Fragments arranged by size: 1524 846 787 597 496 454 393 268 EcoO109I rG'GnC_Cy Cuts at: 2038 2039 2047 2122 2171 2426 2551 2038 Size: 1 8 75 49 255 125 4852 Fragments arranged by size: 4852 255 125 75 49 8 1 EcoRI G'AATT_C Cuts at: 2674 3052 2674 Size: 378 4987 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2068 2416 2068 Size: 348 5017 FspI TGC'GCA Cuts at: 3871 3871 Size: 5365 HaeII r_GCGC'y Cuts at: 1678 2035 2569 4512 1678 Size: 357 534 1943 2531 Fragments arranged by size: 2531 1943 534 357 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4006 4038 4080 4112 1373 Size: 32 135 32 2434 32 42 32 2626 Fragments arranged by size: 2626 2434 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2632 2709 2955 669 Size: 988 281 694 77 246 3079 Fragments arranged by size: 3079 988 694 281 246 77 HindIII A'AGCT_T Cuts at: 748 748 Size: 5365 HpaI GTT'AAC Cuts at: 2955 2955 Size: 5365 KasI G'GCGC_C Cuts at: 2031 2031 Size: 5365 KpnI G_GTAC'C Cuts at: 2690 2690 Size: 5365 MfeI C'AATT_G Cuts at: 2964 2964 Size: 5365 MluI A'CGCG_T Cuts at: 5241 5241 Size: 5365 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4479 4663 1305 Size: 3174 184 2007 Fragments arranged by size: 3174 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3543 Fragments arranged by size: 3543 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2351 2522 3323 3682 3841 518 Size: 648 1185 171 801 359 159 2042 Fragments arranged by size: 2042 1185 801 648 359 171 159 MspA1I CmG'CkG Cuts at: 1606 2578 2781 3458 4606 4851 1606 Size: 972 203 677 1148 245 2120 Fragments arranged by size: 2120 1148 972 677 245 203 NarI GG'CG_CC Cuts at: 2032 2032 Size: 5365 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3874 Fragments arranged by size: 3874 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5365 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5365 NotI GC'GGCC_GC Cuts at: 2812 2812 Size: 5365 NspI r_CATG'y Cuts at: 1132 1229 2326 2344 2723 4268 1132 Size: 97 1097 18 379 1545 2229 Fragments arranged by size: 2229 1545 1097 379 97 18 PasI CC'CwG_GG Cuts at: 1348 2332 1348 Size: 984 4381 PciI A'CATG_T Cuts at: 4264 4264 Size: 5365 PflMI CCAn_nnn'nTGG Cuts at: 1108 2605 1108 Size: 1497 3868 PfoI T'CCnGG_A Cuts at: 2265 2466 2487 2598 2265 Size: 201 21 111 5032 Fragments arranged by size: 5032 201 111 21 PmeI GTTT'AAAC Cuts at: 2669 2669 Size: 5365 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3434 3466 4947 4979 3434 Size: 32 1481 32 3820 Fragments arranged by size: 3820 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2047 2122 2047 Size: 75 5290 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5365 PsiI TTA'TAA Cuts at: 2935 2935 Size: 5365 PspOMI G'GGCC_C Cuts at: 2038 2425 2038 Size: 387 4978 PstI C_TGCA'G Cuts at: 830 2256 2717 830 Size: 1426 461 3478 Fragments arranged by size: 3478 1426 461 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5365 SacI G_AGCT'C Cuts at: 2361 2535 2361 Size: 174 5191 SalI G'TCGA_C Cuts at: 1936 2630 2707 1936 Size: 694 77 4594 Fragments arranged by size: 4594 694 77 SanDI GG'GwC_CC Cuts at: 2122 2122 Size: 5365 SapI GCTCTTCn'nnn_ Cuts at: 2241 2241 Size: 5365 SbfI CC_TGCA'GG Cuts at: 2717 2717 Size: 5365 ScaI AGT'ACT Cuts at: 3613 5325 3613 Size: 1712 3653 SfcI C'TryA_G Cuts at: 826 1045 2252 2713 3848 4529 4720 826 Size: 219 1207 461 1135 681 191 1471 Fragments arranged by size: 1471 1207 1135 681 461 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2549 2549 Size: 5365 SfoI GGC'GCC Cuts at: 2033 2033 Size: 5365 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5365 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5365 SmaI CCC'GGG Cuts at: 1872 2692 1872 Size: 820 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2546 3469 4370 4632 820 Size: 197 926 603 923 901 262 1553 Fragments arranged by size: 1553 926 923 901 603 262 197 SnaBI TAC'GTA Cuts at: 493 3156 493 Size: 2663 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5365 SphI G_CATG'C Cuts at: 1132 2326 2723 1132 Size: 1194 397 3774 Fragments arranged by size: 3774 1194 397 SspI AAT'ATT Cuts at: 5 52 3289 5 Size: 47 3237 2081 Fragments arranged by size: 3237 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5365 StyI C'CwwG_G Cuts at: 513 1065 2004 2075 2109 2804 513 Size: 552 939 71 34 695 3074 Fragments arranged by size: 3074 939 695 552 71 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2225 2292 2303 3392 3409 3562 3747 Size: 1255 645 67 11 1089 17 153 185 Cuts at: 3747 4192 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1089 645 445 185 153 67 17 11 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3611 5323 96 Size: 274 80 33 51 1443 1634 1712 138 Fragments arranged by size: 1712 1634 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5325 5357 5325 Size: 32 5333 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5365 XbaI T'CTAG_A Cuts at: 2701 2701 Size: 5365 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5365 XmaI C'CCGG_G Cuts at: 1870 2690 1870 Size: 820 4545 XmnI GAAnn'nnTTC Cuts at: 3494 3494 Size: 5365 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3173 276 Size: 53 83 186 936 1639 2468 Fragments arranged by size: 2468 1639 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SexAI SrfI SwaI XcmI