Restriction map for
pFN21AE0268
(Linear) (Six-base) MAPSORT of: KIEE0268.seq Check: 4161 from: 1 to: 660
KIEE0268.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 0 626 660
Size: 626 34
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 199 660
Size: 199 461
ApaI G_GGCC'C
Cuts at: 0 37 424 660
Size: 37 387 236
Fragments arranged by size:
387 236 37
AvaI C'yCGr_G
Cuts at: 0 229 660
Size: 229 431
BanI G'GyrC_C
Cuts at: 0 26 310 660
Size: 26 284 350
Fragments arranged by size:
350 284 26
BanII G_rGCy'C
Cuts at: 0 37 79 356 424 530 660
Size: 37 42 277 68 106 130
Fragments arranged by size:
277 130 106 68 42 37
BbeI G_GCGC'C
Cuts at: 0 30 660
Size: 30 630
BbsI GAAGACnn'nnnn_
Cuts at: 0 407 660
Size: 407 253
BbvCI CC'TCA_GC
Cuts at: 0 565 660
Size: 565 95
BglI GCCn_nnn'nGGC
Cuts at: 0 544 660
Size: 544 116
BglII A'GATC_T
Cuts at: 0 362 660
Size: 362 298
Bme1580I G_kGCm'C
Cuts at: 0 37 424 660
Size: 37 387 236
Fragments arranged by size:
387 236 37
BmgBI CAC'GTC
Cuts at: 0 600 660
Size: 600 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 577 660
Size: 81 496 83
Fragments arranged by size:
496 83 81
Bpu10I CC'TnA_GC
Cuts at: 0 565 660
Size: 565 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 660
Size: 526 134
BsaI GGTCTCn'nnnn_
Cuts at: 0 269 660
Size: 269 391
BsaHI Gr'CG_yC
Cuts at: 0 27 131 476 660
Size: 27 104 345 184
Fragments arranged by size:
345 184 104 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 247 660
Size: 247 413
BseYI C'CCAG_C
Cuts at: 0 85 91 190 306 552 660
Size: 85 6 99 116 246 108
Fragments arranged by size:
246 116 108 99 85 6
BsiEI CG_ry'CG
Cuts at: 0 216 510 660
Size: 216 294 150
Fragments arranged by size:
294 216 150
BsiHKAI G_wGCw'C
Cuts at: 0 152 356 530 660
Size: 152 204 174 130
Fragments arranged by size:
204 174 152 130
BsmBI CGTCTCn'nnnn_
Cuts at: 0 433 660
Size: 433 227
Bsp1286I G_dGCh'C
Cuts at: 0 37 79 152 356 424 530 660
Size: 37 42 73 204 68 106 130
Fragments arranged by size:
204 130 106 73 68 42 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 240 660
Size: 240 420
BsrDI GCAATG_nn'
Cuts at: 0 194 660
Size: 194 466
BstBI TT'CG_AA
Cuts at: 0 632 660
Size: 632 28
BstXI CCAn_nnnn'nTGG
Cuts at: 0 348 432 660
Size: 348 84 228
Fragments arranged by size:
348 228 84
BstYI r'GATC_y
Cuts at: 0 362 587 660
Size: 362 225 73
Fragments arranged by size:
362 225 73
Bsu36I CC'TnA_GG
Cuts at: 0 38 660
Size: 38 622
BtsI GCAGTG_nn'
Cuts at: 0 58 660
Size: 58 602
DrdI GACnn_nn'nnGTC
Cuts at: 0 622 660
Size: 622 38
EaeI y'GGCC_r
Cuts at: 0 507 660
Size: 507 153
EagI C'GGCC_G
Cuts at: 0 507 660
Size: 507 153
EarI CTCTTCn'nnn_
Cuts at: 0 236 629 660
Size: 236 393 31
Fragments arranged by size:
393 236 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 398 660
Size: 398 262
EcoICRI GAG'CTC
Cuts at: 0 354 528 660
Size: 354 174 132
Fragments arranged by size:
354 174 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 577 660
Size: 81 496 83
Fragments arranged by size:
496 83 81
EcoO109I rG'GnC_Cy
Cuts at: 0 33 34 42 117 166 421 546 660
Size: 33 1 8 75 49 255 125 114
Fragments arranged by size:
255 125 114 75 49 33 8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 63 411 660
Size: 63 348 249
Fragments arranged by size:
348 249 63
HaeII r_GCGC'y
Cuts at: 0 30 564 660
Size: 30 534 96
Fragments arranged by size:
534 96 30
HincII GTy'rAC
Cuts at: 0 627 660
Size: 627 33
KasI G'GCGC_C
Cuts at: 0 26 660
Size: 26 634
MslI CAynn'nnrTG
Cuts at: 0 346 517 660
Size: 346 171 143
Fragments arranged by size:
346 171 143
MspA1I CmG'CkG
Cuts at: 0 573 660
Size: 573 87
NarI GG'CG_CC
Cuts at: 0 27 660
Size: 27 633
NspI r_CATG'y
Cuts at: 0 321 339 660
Size: 321 18 321
Fragments arranged by size:
321 321 18
PasI CC'CwG_GG
Cuts at: 0 327 660
Size: 327 333
PflMI CCAn_nnn'nTGG
Cuts at: 0 600 660
Size: 600 60
PfoI T'CCnGG_A
Cuts at: 0 260 461 482 593 660
Size: 260 201 21 111 67
Fragments arranged by size:
260 201 111 67 21
PpuMI rG'GwC_Cy
Cuts at: 0 42 117 660
Size: 42 75 543
Fragments arranged by size:
543 75 42
PspOMI G'GGCC_C
Cuts at: 0 33 420 660
Size: 33 387 240
Fragments arranged by size:
387 240 33
PstI C_TGCA'G
Cuts at: 0 251 660
Size: 251 409
SacI G_AGCT'C
Cuts at: 0 356 530 660
Size: 356 174 130
Fragments arranged by size:
356 174 130
SalI G'TCGA_C
Cuts at: 0 625 660
Size: 625 35
SanDI GG'GwC_CC
Cuts at: 0 117 660
Size: 117 543
SapI GCTCTTCn'nnn_
Cuts at: 0 236 660
Size: 236 424
SfcI C'TryA_G
Cuts at: 0 247 660
Size: 247 413
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 544 660
Size: 544 116
SfoI GGC'GCC
Cuts at: 0 28 660
Size: 28 632
SmlI C'TyrA_G
Cuts at: 0 541 660
Size: 541 119
SphI G_CATG'C
Cuts at: 0 321 660
Size: 321 339
StyI C'CwwG_G
Cuts at: 0 70 104 660
Size: 70 34 556
Fragments arranged by size:
556 70 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 220 287 298 660
Size: 220 67 11 362
Fragments arranged by size:
362 220 67 11
Enzymes that do cut:
AccI AlfI ApaI AvaI BanI BanII BbeI BbsI
BbvCI BglI BglII Bme1580I BmgBI BpmI Bpu10I BpuEI
BsaI BsaHI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrDI BstBI BstXI BstYI Bsu36I BtsI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI
HaeII HincII KasI MslI MspA1I NarI NspI PasI
PflMI PfoI PpuMI PspOMI PstI SacI SalI SanDI
SapI SfcI SfiI SfoI SmlI SphI StyI TaqII
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BlpI BmrI BmtI BplI BsaAI BsaBI BsaWI
BsaXI BsgI BsiWI BsmI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstZ17I BtgI BtgZI
ClaI CspCI DraI DraIII EcoNI EcoRI EcoRV FseI
FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI
MmeI MscI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacII SbfI
ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI