(Circular) (Six-base) MAPSORT of: pFN21AE0266.seq Check: 7514 from: 1 to: 6055 pFN21AE0266 6055 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2790 2790 Size: 6055 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2682 3865 278 Size: 53 83 186 936 1146 1183 2468 Fragments arranged by size: 2468 1183 1146 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3398 1937 Size: 1461 4594 Acc65I G'GTAC_C Cuts at: 3376 3376 Size: 6055 AclI AA'CG_TT Cuts at: 1563 4182 4555 1563 Size: 2619 373 3063 Fragments arranged by size: 3063 2619 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3141 4118 5502 5770 1632 Size: 1509 977 1384 268 1917 Fragments arranged by size: 1917 1509 1384 977 268 AfeI AGC'GCT Cuts at: 3245 3245 Size: 6055 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5858 AflIII A'CryG_T Cuts at: 1203 2721 4954 5931 1203 Size: 1518 2233 977 1327 Fragments arranged by size: 2233 1518 1327 977 AgeI A'CCGG_T Cuts at: 4886 4886 Size: 6055 AhdI GACnn_n'nnGTC Cuts at: 4784 4784 Size: 6055 AleI CACnn'nnGTG Cuts at: 1166 2402 1166 Size: 1236 4819 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2750 1184 Size: 1566 4489 AlwNI CAG_nnn'CTG Cuts at: 2167 2230 2854 3352 3798 5370 2167 Size: 63 624 498 446 1572 2852 Fragments arranged by size: 2852 1572 624 498 446 63 ApaI G_GGCC'C Cuts at: 3019 3149 3019 Size: 130 5925 ApaLI G'TGCA_C Cuts at: 4112 5268 4112 Size: 1156 4899 ApoI r'AATT_y Cuts at: 1493 3364 3590 3742 5851 1493 Size: 1871 226 152 2109 1697 Fragments arranged by size: 2109 1871 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4609 160 Size: 872 3577 1606 Fragments arranged by size: 3577 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 AvaI C'yCGr_G Cuts at: 1870 1943 3380 1870 Size: 73 1437 4545 Fragments arranged by size: 4545 1437 73 BamHI G'GATC_C Cuts at: 3385 3752 3385 Size: 367 5688 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2423 2541 3376 4831 618 Size: 325 217 627 636 118 835 1455 1842 Fragments arranged by size: 1842 1455 835 636 627 325 217 118 BanII G_rGCy'C Cuts at: 1391 2013 2670 3019 3072 3149 3374 1391 Size: 622 657 349 53 77 225 4072 Fragments arranged by size: 4072 657 622 349 225 77 53 BbeI G_GCGC'C Cuts at: 2427 2427 Size: 6055 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2836 928 Size: 971 937 4147 Fragments arranged by size: 4147 971 937 BbvCI CC'TCA_GC Cuts at: 2027 2079 2027 Size: 52 6003 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3153 3187 4246 4280 1903 Size: 34 1216 34 1059 34 3678 Fragments arranged by size: 3678 1216 1059 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2241 3947 5157 2241 Size: 1706 1210 3139 Fragments arranged by size: 3139 1706 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6055 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2421 2582 2632 Size: 107 122 71 631 605 749 161 50 Cuts at: 2632 3076 4666 136 Size: 444 1590 1525 Fragments arranged by size: 1590 1525 749 631 605 444 161 122 107 71 50 BglII A'GATC_T Cuts at: 6050 6050 Size: 6055 BlpI GC'TnA_GC Cuts at: 3472 3472 Size: 6055 Bme1580I G_kGCm'C Cuts at: 1790 2137 2544 3019 3149 4116 5272 1790 Size: 347 407 475 130 967 1156 2573 Fragments arranged by size: 2573 1156 967 475 407 347 130 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6055 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2979 2990 3106 Size: 416 53 83 399 24 1562 11 116 Cuts at: 3106 3786 4744 442 Size: 680 958 1753 Fragments arranged by size: 1753 1562 958 680 416 399 116 83 53 24 11 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6055 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2201 2367 4715 1239 Size: 962 166 2348 2579 Fragments arranged by size: 2579 2348 962 166 Bpu10I CC'TnA_GC Cuts at: 2027 2079 2027 Size: 52 6003 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2081 4180 5045 5343 2081 Size: 2099 865 298 2793 Fragments arranged by size: 2793 2099 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2134 4718 882 Size: 617 635 2584 2219 Fragments arranged by size: 2584 2219 635 617 BsaAI yAC'GTr Cuts at: 493 1204 2722 3199 3846 493 Size: 711 1518 477 647 2702 Fragments arranged by size: 2702 1518 711 647 477 BsaBI GATnn'nnATC Cuts at: 6049 6049 Size: 6055 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2424 2496 2679 2817 Size: 53 83 186 936 891 72 183 138 Cuts at: 2817 3862 4244 4879 275 Size: 1045 382 635 1451 Fragments arranged by size: 1451 1045 936 891 635 382 186 183 138 83 72 53 BsaWI w'CCGG_w Cuts at: 2013 4488 4886 5160 5307 5718 2013 Size: 2475 398 274 147 411 2350 Fragments arranged by size: 2475 2350 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5830 Fragments arranged by size: 5830 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6055 BseYI C'CCAG_C Cuts at: 2324 2433 2499 2617 2812 3025 3139 3240 5258 Size: 109 66 118 195 213 114 101 2018 Cuts at: 5258 5754 2324 Size: 496 2625 Fragments arranged by size: 2625 2018 496 213 195 118 114 109 101 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3176 3365 1163 Size: 573 1440 189 3853 Fragments arranged by size: 3853 1440 573 189 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2561 2880 3505 4266 5294 1540 Size: 275 186 560 319 625 761 1028 2301 Fragments arranged by size: 2301 1028 761 625 560 319 275 186 BsiHKAI G_wGCw'C Cuts at: 2646 2751 4116 4201 5272 2646 Size: 105 1365 85 1071 3429 Fragments arranged by size: 3429 1365 1071 105 85 BsmI GAATG_Cn' Cuts at: 3566 3659 3566 Size: 93 5962 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2137 2544 2646 2670 2751 3019 Size: 399 223 124 407 102 24 81 268 Cuts at: 3019 3072 3149 3374 4116 4201 5272 1391 Size: 53 77 225 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 407 399 268 225 223 124 102 85 81 77 53 24 BspEI T'CCGG_A Cuts at: 2013 5718 2013 Size: 3705 2350 BspHI T'CATG_A Cuts at: 2961 3324 3944 5674 2961 Size: 363 620 1730 3342 Fragments arranged by size: 3342 1730 620 363 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2739 2790 2976 3410 844 Size: 1895 51 186 434 3489 Fragments arranged by size: 3489 1895 434 186 51 BsrBI CCG'CTC Cuts at: 1394 1823 2770 2893 3942 1394 Size: 429 947 123 1049 3507 Fragments arranged by size: 3507 1049 947 429 123 BsrDI GCAATG_nn' Cuts at: 65 1248 3207 4550 4724 65 Size: 1183 1959 1343 174 1396 Fragments arranged by size: 1959 1396 1343 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 2443 2626 2875 4699 4886 1700 Size: 110 275 358 183 249 1824 187 2869 Fragments arranged by size: 2869 1824 358 275 249 187 183 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6055 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6055 BssSI C'ACGA_G Cuts at: 2461 3155 4115 5127 2461 Size: 694 960 1012 3389 Fragments arranged by size: 3389 1012 960 694 BstAPI GCAn_nnn'nTGC Cuts at: 794 2463 794 Size: 1669 4386 BstBI TT'CG_AA Cuts at: 4859 4859 Size: 6055 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6055 BstXI CCAn_nnnn'nTGG Cuts at: 3319 3319 Size: 6055 BstYI r'GATC_y Cuts at: 1972 3385 3752 4137 4154 5606 5692 5704 6050 Size: 1413 367 385 17 1452 86 12 346 Cuts at: 6050 1972 Size: 1977 Fragments arranged by size: 1977 1452 1413 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2925 3312 513 Size: 552 939 921 387 3256 Fragments arranged by size: 3256 939 921 552 387 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5316 Fragments arranged by size: 5316 683 56 BtsI GCAGTG_nn' Cuts at: 2503 3571 4365 4385 2503 Size: 1068 794 20 4173 Fragments arranged by size: 4173 1068 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6055 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6020 DraI TTT'AAA Cuts at: 3359 3359 Size: 6055 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5062 809 Size: 507 3746 1802 Fragments arranged by size: 3746 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2342 2445 2558 Size: 54 1425 50 275 18 512 103 113 Cuts at: 2558 2859 2877 2913 3297 3502 4391 6020 8 Size: 301 18 36 384 205 889 1629 43 Fragments arranged by size: 1629 1425 889 512 384 301 275 205 113 103 54 50 43 36 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2558 2877 3502 1537 Size: 275 746 319 625 4090 Fragments arranged by size: 4090 746 625 319 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3985 1353 Size: 2632 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2380 2600 2981 4642 5016 5162 Size: 1064 138 791 220 381 1661 374 146 Cuts at: 5162 387 Size: 1280 Fragments arranged by size: 1661 1280 1064 791 381 374 220 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2367 3141 4118 4715 5502 5770 Size: 393 569 166 774 977 597 787 268 Cuts at: 5770 1239 Size: 1524 Fragments arranged by size: 1524 977 787 774 597 569 393 268 166 EcoNI CCTnn'n_nnAGG Cuts at: 3010 3123 3010 Size: 113 5942 EcoO109I rG'GnC_Cy Cuts at: 2284 3015 3016 3043 3146 3349 2284 Size: 731 1 27 103 203 4990 Fragments arranged by size: 4990 731 203 103 27 1 EcoRI G'AATT_C Cuts at: 3364 3742 3364 Size: 378 5677 FspI TGC'GCA Cuts at: 4561 4561 Size: 6055 HaeII r_GCGC'y Cuts at: 1678 2427 2571 2837 3247 3268 5202 1678 Size: 749 144 266 410 21 1934 2531 Fragments arranged by size: 2531 1934 749 410 266 144 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2224 2256 2792 2824 4696 Size: 32 135 32 652 32 536 32 1872 Cuts at: 4696 4728 4770 4802 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1872 652 536 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3399 3645 669 Size: 988 281 1461 246 3079 Fragments arranged by size: 3079 1461 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6055 HpaI GTT'AAC Cuts at: 3645 3645 Size: 6055 KasI G'GCGC_C Cuts at: 2423 2423 Size: 6055 KpnI G_GTAC'C Cuts at: 3380 3380 Size: 6055 MfeI C'AATT_G Cuts at: 3654 3654 Size: 6055 MluI A'CGCG_T Cuts at: 5931 5931 Size: 6055 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5169 5353 1305 Size: 3864 184 2007 Fragments arranged by size: 3864 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2915 3299 10 Size: 54 1425 343 1083 384 2766 Fragments arranged by size: 2766 1425 1083 384 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2207 2402 2976 4013 4372 4531 518 Size: 648 1041 195 574 1037 359 159 2042 Fragments arranged by size: 2042 1041 1037 648 574 359 195 159 MspA1I CmG'CkG Cuts at: 1606 3143 3471 4148 5296 5541 1606 Size: 1537 328 677 1148 245 2120 Fragments arranged by size: 2120 1537 1148 677 328 245 NaeI GCC'GGC Cuts at: 2087 2445 2628 2087 Size: 358 183 5514 Fragments arranged by size: 5514 358 183 NarI GG'CG_CC Cuts at: 2424 2424 Size: 6055 NcoI C'CATG_G Cuts at: 513 1065 2004 2925 3312 513 Size: 552 939 921 387 3256 Fragments arranged by size: 3256 939 921 552 387 NdeI CA'TA_TG Cuts at: 387 387 Size: 6055 NgoMIV G'CCGG_C Cuts at: 2085 2443 2626 2085 Size: 358 183 5514 Fragments arranged by size: 5514 358 183 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6055 NotI GC'GGCC_GC Cuts at: 2558 3502 2558 Size: 944 5111 NspI r_CATG'y Cuts at: 1132 1229 2626 2872 3413 4958 1132 Size: 97 1397 246 541 1545 2229 Fragments arranged by size: 2229 1545 1397 541 246 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6055 PciI A'CATG_T Cuts at: 4954 4954 Size: 6055 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6055 PfoI T'CCnGG_A Cuts at: 2525 2525 Size: 6055 PmeI GTTT'AAAC Cuts at: 3359 3359 Size: 6055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4124 4156 5637 5669 4124 Size: 32 1481 32 4510 Fragments arranged by size: 4510 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2284 2284 Size: 6055 PshAI GACnn'nnGTC Cuts at: 1613 2927 1613 Size: 1314 4741 PsiI TTA'TAA Cuts at: 3625 3625 Size: 6055 PspOMI G'GGCC_C Cuts at: 3015 3145 3015 Size: 130 5925 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2957 2989 2957 Size: 32 6023 PstI C_TGCA'G Cuts at: 830 2065 2575 3407 830 Size: 1235 510 832 3478 Fragments arranged by size: 3478 1235 832 510 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6055 SalI G'TCGA_C Cuts at: 1936 3397 1936 Size: 1461 4594 SanDI GG'GwC_CC Cuts at: 2284 2284 Size: 6055 SbfI CC_TGCA'GG Cuts at: 3407 3407 Size: 6055 ScaI AGT'ACT Cuts at: 2906 4303 6015 2906 Size: 1397 1712 2946 Fragments arranged by size: 2946 1712 1397 SexAI A'CCwGG_T Cuts at: 2223 2223 Size: 6055 SfcI C'TryA_G Cuts at: 826 1045 2061 2571 3403 4538 5219 5410 826 Size: 219 1016 510 832 1135 681 191 1471 Fragments arranged by size: 1471 1135 1016 832 681 510 219 191 SfoI GGC'GCC Cuts at: 2425 2425 Size: 6055 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6055 SmaI CCC'GGG Cuts at: 1872 3382 1872 Size: 1510 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2096 4159 5060 5322 820 Size: 197 926 153 2063 901 262 1553 Fragments arranged by size: 2063 1553 926 901 262 197 153 SnaBI TAC'GTA Cuts at: 493 3846 493 Size: 3353 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6055 SphI G_CATG'C Cuts at: 1132 2626 2872 3413 1132 Size: 1494 246 541 3774 Fragments arranged by size: 3774 1494 541 246 SspI AAT'ATT Cuts at: 5 52 3979 5 Size: 47 3927 2081 Fragments arranged by size: 3927 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6055 StyI C'CwwG_G Cuts at: 513 1065 2004 2925 3312 3494 513 Size: 552 939 921 387 182 3074 Fragments arranged by size: 3074 939 921 552 387 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4082 4099 4252 4437 4882 325 Size: 1255 2502 17 153 185 445 1498 Fragments arranged by size: 2502 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2904 4301 6013 Size: 274 80 33 51 1443 927 1397 1712 Cuts at: 6013 96 Size: 138 Fragments arranged by size: 1712 1443 1397 927 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2937 2969 6015 6047 2937 Size: 32 3046 32 2945 Fragments arranged by size: 3046 2945 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2191 1316 Size: 875 5180 XbaI T'CTAG_A Cuts at: 3391 3391 Size: 6055 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2401 2923 3003 2401 Size: 522 80 5453 Fragments arranged by size: 5453 522 80 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6055 XmaI C'CCGG_G Cuts at: 1870 3380 1870 Size: 1510 4545 XmnI GAAnn'nnTTC Cuts at: 4184 4184 Size: 6055 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2680 3863 276 Size: 53 83 186 936 1146 1183 2468 Fragments arranged by size: 2468 1183 1146 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PvuII RsrII SacI SacII SapI SfiI SrfI SwaI