Restriction map for
pFN21AE0264
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BspDI BspEI
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HgaI HindIII HpaI
KpnI KspI MaeII MamI MflI
MluI MroI NcoI NdeI NheI
NotI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 902 GTAGAC GT^[AC][GT]AC
AcsI 1035 AAATTT [GA]^AATT[CT]
AcyI 21 GGCGCC G[GA]^CG[CT]C
AflII 759 CTTAAG C^TTAAG
AflIII 94 ACATGT A^C[GA][CT]GT
AlwI 450 GGATC GGATC(4/5)
ApoI 1035 AAATTT [GA]^AATT[CT]
Asp700I 139 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 238 CTCGAG C^[CT]CG[GA]G
BanI 21 GGCGCC G^G[CT][GA]CC
BanII 338 GAGCCC G[GA]GC[CT]^C
BbeI 21 GGCGCC GGCGC^C
BcnI 516 CCGGG CC^[GC]GG
BfaI 671 CTAG C^TAG
BfrI 759 CTTAAG C^TTAAG
BsaHI 21 GGCGCC G[GA]^CG[CT]C
BsaI 81 GAGACC GGTCTC(1/5)
BseRI 1105 CTCCTC GAGGAG(10/8)
BsmI 104 GCATTC GAATGC(1/-1)
BsoBI 238 CTCGAG C^[CT]CG[GA]G
Bsp1407I 278 TGTACA T^GTACA
BspHI 1187 TCATGA T^CATGA
BspLU11I 94 ACATGT A^CATGT
BspMI 705 ACCTGC ACCTGC(4/8)
BsrFI 955 GCCGGC [GA]^CCGG[CT]
BsrGI 278 TGTACA T^GTACA
BstAPI 1091 GCAAGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 955 GCCGGC [GA]^CCGG[CT]
Cfr13I 1137 GGGCC G^G[ACGT]CC
EarI 1112 GAAGAG CTCTTC(1/4)
HaeII 21 GGCGCC [GA]GCGC^[CT]
Hin1I 21 GGCGCC G[GA]^CG[CT]C
KasI 21 GGCGCC G^GCGCC
Ksp632I 1112 GAAGAG CTCTTC(1/4)
MaeI 671 CTAG C^TAG
MfeI 713 CAATTG C^AATTG
MunI 713 CAATTG C^AATTG
NaeI 955 GCCGGC GCC^GGC
NarI 21 GGCGCC GG^CGCC
NciI 516 CCGGG CC^[GC]GG
NgoMIV 955 GCCGGC G^CCGGC
NruI 981 TCGCGA TCG^CGA
NspI 94 ACATGT [GA]CATG^[CT]
PaeR7I 238 CTCGAG C^TCGAG
PshAI 1080 GACTGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 395 CAGCTG CAG^CTG
RcaI 1187 TCATGA T^CATGA
Sau96I 1137 GGGCC G^G[ACGT]CC
SbfI 526 CCTGCAGG CCTGCA^GG
SfoI 21 GGCGCC GGC^GCC
Sse8387I 526 CCTGCAGG CCTGCA^GG
SspBI 278 TGTACA T^GTACA
XhoI 238 CTCGAG C^TCGAG
XmnI 139 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
BmyI 338 GAGCCC G[AGT]GC[ACT]^C
1098 GTGCCC
BsgI 707 CTGCAC GTGCAG(16/14)
370 GTGCAG
BsiYI 33 CCTGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
500 CCCCCAACTGG
BslI 33 CCTGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
500 CCCCCAACTGG
BsmAI 81 GAGAC GTCTC(1/5)
1006 GTCTC
BsmFI 913 GGGAC GGGAC(10/14)
72 GTCCC
Bsp1286I 338 GAGCCC G[AGT]GC[ACT]^C
1098 GTGCCC
BsrDI 401 GCAATG GCAATG(2/0)
1239 GCAATG
BstF5I 273 GGATG GGATG(2/0)
1221 GGATG
BstXI 288 CCATGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
300 CCAGCGCATTGG
DdeI 722 CTGAG C^T[ACGT]AG
1108 CTCAG
DpnII 451 GATC ^GATC
1011 GATC
DraI 493 TTTAAA TTT^AAA
867 TTTAAA
DsaI 860 CCACGG C^C[GA][CT]GG
878 CCGTGG
FauI 653 GCGGG CCCGC(4/6)
1054 GCGGG
FokI 273 GGATG GGATG(9/13)
1221 GGATG
HincII 661 GTCAAC GT[CT]^[GA]AC
890 GTCAAC
HindII 661 GTCAAC GT[CT]^[GA]AC
890 GTCAAC
MboI 451 GATC ^GATC
1011 GATC
MspA1I 395 CAGCTG C[AC]G^C[GT]G
425 CCGCTG
NdeII 451 GATC ^GATC
1011 GATC
NlaIV 21 GGCGCC GG[ACGT]^[ACGT]CC
337 GGAGCC
PstI 527 CTGCAG CTGCA^G
770 CTGCAG
Sau3AI 451 GATC ^GATC
1011 GATC
SmlI 238 CTCGAG C^T[CT][GA]AG
759 CTTAAG
TaqI 239 TCGA T^CGA
1009 TCGA
TthHB8I 239 TCGA T^CGA
1009 TCGA
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AfaI AluI BalI
BbvI BpmI BsaJI BsrI BssKI
BstNI BstUI Cac8I CfoI EaeI
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboII MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NlaIII PleI RsaI ScrFI
SfaNI SfcI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI