(Circular) (Six-base) MAPSORT of: pFN21AE0264.seq Check: 1276 from: 1 to: 5950 pFN21AE0264 5950 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2719 2719 Size: 5950 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3760 278 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2908 3293 1937 Size: 971 385 4594 Fragments arranged by size: 4594 971 385 Acc65I G'GTAC_C Cuts at: 3271 3271 Size: 5950 AclI AA'CG_TT Cuts at: 1563 4077 4450 1563 Size: 2514 373 3063 Fragments arranged by size: 3063 2514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4013 5397 5665 1632 Size: 2381 1384 268 1917 Fragments arranged by size: 2381 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2764 820 Size: 197 1747 4006 Fragments arranged by size: 4006 1747 197 AflIII A'CryG_T Cuts at: 1203 2099 4849 5826 1203 Size: 896 2750 977 1327 Fragments arranged by size: 2750 1327 977 896 AgeI A'CCGG_T Cuts at: 4781 4781 Size: 5950 AhdI GACnn_n'nnGTC Cuts at: 4679 4679 Size: 5950 AleI CACnn'nnGTG Cuts at: 1166 2546 1166 Size: 1380 4570 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3191 1184 Size: 2007 3943 AlwNI CAG_nnn'CTG Cuts at: 3693 5265 3693 Size: 1572 4378 ApaLI G'TGCA_C Cuts at: 4007 5163 4007 Size: 1156 4794 ApoI r'AATT_y Cuts at: 1493 3040 3259 3485 3637 5746 1493 Size: 1547 219 226 152 2109 1697 Fragments arranged by size: 2109 1697 1547 226 219 152 AseI AT'TA_AT Cuts at: 160 1032 4504 160 Size: 872 3472 1606 Fragments arranged by size: 3472 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 AvaI C'yCGr_G Cuts at: 1870 1943 2243 3275 1870 Size: 73 300 1032 4545 Fragments arranged by size: 4545 1032 300 73 BamHI G'GATC_C Cuts at: 3280 3647 3280 Size: 367 5583 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 3271 4726 618 Size: 325 217 627 239 1245 1455 1842 Fragments arranged by size: 1842 1455 1245 627 325 239 217 BanII G_rGCy'C Cuts at: 1391 2347 3269 1391 Size: 956 922 4072 Fragments arranged by size: 4072 956 922 BbeI G_GCGC'C Cuts at: 2030 2030 Size: 5950 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4979 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4141 4175 1903 Size: 34 2204 34 3678 Fragments arranged by size: 3678 2204 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3842 5052 3842 Size: 1210 4740 BclI T'GATC_A Cuts at: 1548 1548 Size: 5950 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4561 136 Size: 107 122 71 631 605 2889 1525 Fragments arranged by size: 2889 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5945 5945 Size: 5950 BlpI GC'TnA_GC Cuts at: 3367 3367 Size: 5950 Bme1580I G_kGCm'C Cuts at: 1790 3107 4011 5167 1790 Size: 1317 904 1156 2573 Fragments arranged by size: 2573 1317 1156 904 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5950 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3681 4639 442 Size: 416 53 83 399 24 2264 958 1753 Fragments arranged by size: 2264 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2139 2175 2361 2945 4610 1239 Size: 900 36 186 584 1665 2579 Fragments arranged by size: 2579 1665 900 584 186 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4075 4940 5238 4075 Size: 865 298 4787 Fragments arranged by size: 4787 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2080 4613 882 Size: 617 581 2533 2219 Fragments arranged by size: 2533 2219 617 581 BsaAI yAC'GTr Cuts at: 493 1204 3741 493 Size: 711 2537 2702 Fragments arranged by size: 2702 2537 711 BsaBI GATnn'nnATC Cuts at: 5944 5944 Size: 5950 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2027 3757 4139 4774 Size: 53 83 186 936 494 1730 382 635 Cuts at: 4774 275 Size: 1451 Fragments arranged by size: 1730 1451 936 635 494 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4383 4781 5055 5202 5613 4383 Size: 398 274 147 411 4720 Fragments arranged by size: 4720 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2334 2364 1178 Size: 30 165 30 931 30 4764 Fragments arranged by size: 4764 931 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3101 1186 Size: 1915 4035 BseYI C'CCAG_C Cuts at: 2058 5153 5649 2058 Size: 3095 496 2359 Fragments arranged by size: 3095 2359 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2396 2697 1163 Size: 573 660 301 4416 Fragments arranged by size: 4416 660 573 301 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3400 4161 5189 1540 Size: 275 186 1399 761 1028 2301 Fragments arranged by size: 2301 1399 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4011 4096 5167 4011 Size: 85 1071 4794 Fragments arranged by size: 4794 1071 85 BsmI GAATG_Cn' Cuts at: 2109 3461 3554 2109 Size: 1352 93 4505 Fragments arranged by size: 4505 1352 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2347 3107 3269 4011 4096 5167 1391 Size: 399 557 760 162 742 85 1071 2174 Fragments arranged by size: 2174 1071 760 742 557 399 162 85 BspEI T'CCGG_A Cuts at: 5613 5613 Size: 5950 BspHI T'CATG_A Cuts at: 3192 3839 5569 3192 Size: 647 1730 3573 Fragments arranged by size: 3573 1730 647 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2719 3305 844 Size: 1875 586 3489 Fragments arranged by size: 3489 1875 586 BsrBI CCG'CTC Cuts at: 1394 1823 3837 1394 Size: 429 2014 3507 Fragments arranged by size: 3507 2014 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2413 3251 4445 4619 65 Size: 1183 1165 838 1194 174 1396 Fragments arranged by size: 1396 1194 1183 1165 838 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2960 4594 4781 1700 Size: 110 1150 1634 187 2869 Fragments arranged by size: 2869 1634 1150 187 110 BsrGI T'GTAC_A Cuts at: 96 2283 96 Size: 2187 3763 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5950 BssSI C'ACGA_G Cuts at: 4010 5022 4010 Size: 1012 4938 BstAPI GCAn_nnn'nTGC Cuts at: 794 3102 794 Size: 2308 3642 BstBI TT'CG_AA Cuts at: 4754 4754 Size: 5950 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5950 BstXI CCAn_nnnn'nTGG Cuts at: 2300 2312 2300 Size: 12 5938 BstYI r'GATC_y Cuts at: 1972 3280 3647 4032 4049 5501 5587 5599 5945 Size: 1308 367 385 17 1452 86 12 346 Cuts at: 5945 1972 Size: 1977 Fragments arranged by size: 1977 1452 1308 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2865 2883 513 Size: 552 1800 18 3580 Fragments arranged by size: 3580 1800 552 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3003 487 Size: 683 56 1777 3434 Fragments arranged by size: 3434 1777 683 56 BtsI GCAGTG_nn' Cuts at: 2146 3107 3466 4260 4280 2146 Size: 961 359 794 20 3816 Fragments arranged by size: 3816 961 794 359 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5950 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5915 DraI TTT'AAA Cuts at: 2500 2874 3254 2500 Size: 374 380 5196 Fragments arranged by size: 5196 380 374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4957 809 Size: 507 3641 1802 Fragments arranged by size: 3641 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2290 2302 2314 Size: 54 1425 50 275 18 460 12 12 Cuts at: 2314 2418 2513 3397 4286 5915 8 Size: 104 95 884 889 1629 43 Fragments arranged by size: 1629 1425 889 884 460 275 104 95 54 50 43 18 12 12 EagI C'GGCC_G Cuts at: 1537 1812 3397 1537 Size: 275 1585 4090 Fragments arranged by size: 4090 1585 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3112 3880 1353 Size: 1759 768 3423 Fragments arranged by size: 3423 1759 768 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4537 4911 5057 387 Size: 1064 138 2948 374 146 1280 Fragments arranged by size: 2948 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2139 2175 2361 2945 4013 4610 5397 Size: 393 507 36 186 584 1068 597 787 Cuts at: 5397 5665 1239 Size: 268 1524 Fragments arranged by size: 1524 1068 787 597 584 507 393 268 186 36 EcoRI G'AATT_C Cuts at: 3259 3637 3259 Size: 378 5572 FspI TGC'GCA Cuts at: 4456 4456 Size: 5950 HaeII r_GCGC'y Cuts at: 1678 2030 5097 1678 Size: 352 3067 2531 Fragments arranged by size: 3067 2531 352 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2905 2937 3156 3188 3196 Size: 32 135 32 1333 32 219 32 8 Cuts at: 3196 3228 4591 4623 4665 4697 1373 Size: 32 1363 32 42 32 2626 Fragments arranged by size: 2626 1363 1333 219 135 42 32 32 32 32 32 32 32 8 HincII GTy'rAC Cuts at: 669 1657 1938 2668 2897 3294 3540 669 Size: 988 281 730 229 397 246 3079 Fragments arranged by size: 3079 988 730 397 281 246 229 HindIII A'AGCT_T Cuts at: 748 748 Size: 5950 HpaI GTT'AAC Cuts at: 3540 3540 Size: 5950 KasI G'GCGC_C Cuts at: 2026 2026 Size: 5950 KpnI G_GTAC'C Cuts at: 3275 3275 Size: 5950 MfeI C'AATT_G Cuts at: 2718 3549 2718 Size: 831 5119 MluI A'CGCG_T Cuts at: 5826 5826 Size: 5950 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5064 5248 1305 Size: 3759 184 2007 Fragments arranged by size: 3759 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2292 2304 2420 2515 10 Size: 54 1425 343 460 12 116 95 3445 Fragments arranged by size: 3445 1425 460 343 116 95 54 12 MslI CAynn'nnrTG Cuts at: 518 1166 2298 2420 2546 3137 3908 4267 4426 Size: 648 1132 122 126 591 771 359 159 Cuts at: 4426 518 Size: 2042 Fragments arranged by size: 2042 1132 771 648 591 359 159 126 122 MspA1I CmG'CkG Cuts at: 1606 2402 2432 3366 4043 5191 5436 1606 Size: 796 30 934 677 1148 245 2120 Fragments arranged by size: 2120 1148 934 796 677 245 30 NaeI GCC'GGC Cuts at: 2962 2962 Size: 5950 NarI GG'CG_CC Cuts at: 2027 2027 Size: 5950 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5398 NdeI CA'TA_TG Cuts at: 387 387 Size: 5950 NgoMIV G'CCGG_C Cuts at: 2960 2960 Size: 5950 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5950 NotI GC'GGCC_GC Cuts at: 3397 3397 Size: 5950 NruI TCG'CGA Cuts at: 2988 2988 Size: 5950 NspI r_CATG'y Cuts at: 1132 1229 2103 3308 4853 1132 Size: 97 874 1205 1545 2229 Fragments arranged by size: 2229 1545 1205 874 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5950 PciI A'CATG_T Cuts at: 2099 4849 2099 Size: 2750 3200 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5950 PfoI T'CCnGG_A Cuts at: 2152 3222 2152 Size: 1070 4880 PmeI GTTT'AAAC Cuts at: 3254 3254 Size: 5950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4019 4051 5532 5564 4019 Size: 32 1481 32 4405 Fragments arranged by size: 4405 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3089 1613 Size: 1476 4474 PsiI TTA'TAA Cuts at: 3520 3520 Size: 5950 PstI C_TGCA'G Cuts at: 830 2536 2779 3302 830 Size: 1706 243 523 3478 Fragments arranged by size: 3478 1706 523 243 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5950 PvuII CAG'CTG Cuts at: 2402 2402 Size: 5950 SalI G'TCGA_C Cuts at: 1936 3292 1936 Size: 1356 4594 SbfI CC_TGCA'GG Cuts at: 2536 3302 2536 Size: 766 5184 ScaI AGT'ACT Cuts at: 4198 5910 4198 Size: 1712 4238 SfcI C'TryA_G Cuts at: 826 1045 2532 2775 2829 3298 4433 5114 5305 Size: 219 1487 243 54 469 1135 681 191 Cuts at: 5305 826 Size: 1471 Fragments arranged by size: 1487 1471 1135 681 469 243 219 191 54 SfoI GGC'GCC Cuts at: 2028 2028 Size: 5950 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5950 SmaI CCC'GGG Cuts at: 1872 3277 1872 Size: 1405 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2243 2764 4054 4955 5217 820 Size: 197 926 300 521 1290 901 262 1553 Fragments arranged by size: 1553 1290 926 901 521 300 262 197 SnaBI TAC'GTA Cuts at: 493 3741 493 Size: 3248 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5950 SphI G_CATG'C Cuts at: 1132 3308 1132 Size: 2176 3774 SspI AAT'ATT Cuts at: 5 52 3874 5 Size: 47 3822 2081 Fragments arranged by size: 3822 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5950 StyI C'CwwG_G Cuts at: 513 1065 3389 513 Size: 552 2324 3074 Fragments arranged by size: 3074 2324 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2072 3977 3994 4147 4332 4777 325 Size: 1255 492 1905 17 153 185 445 1498 Fragments arranged by size: 1905 1498 1255 492 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2180 2283 4196 Size: 274 80 33 51 1443 203 103 1913 Cuts at: 4196 5908 96 Size: 1712 138 Fragments arranged by size: 1913 1712 1443 274 203 138 103 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2334 2366 5910 5942 2334 Size: 32 3544 32 2342 Fragments arranged by size: 3544 2342 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5950 XbaI T'CTAG_A Cuts at: 3286 3286 Size: 5950 XhoI C'TCGA_G Cuts at: 1943 2243 1943 Size: 300 5650 XmaI C'CCGG_G Cuts at: 1870 3275 1870 Size: 1405 4545 XmnI GAAnn'nnTTC Cuts at: 2148 4079 2148 Size: 1931 4019 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3758 276 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI