Restriction map for
pFN21AE0263
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# NO CUT ENZYMES ( 205 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaJI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HaeIII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MslI MspA1I MunI MvnI
MwoI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI TaqI
Tru9I Tsp45I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AspHI 246 GAGCAC G[AT]GC[AT]^C
BanII 7 GAGCCC G[GA]GC[CT]^C
BbvI 85 GCAGC GCAGC(8/12)
BcnI 89 CCCGG CC^[GC]GG
BglII 185 AGATCT A^GATCT
BpmI 36 CTCCAG CTGGAG(16/14)
BsaI 33 GGTCTC GGTCTC(1/5)
BsaWI 233 ACCGGA [AT]^CCGG[AT]
BsiHKAI 246 GAGCAC G[AT]GC[AT]^C
BstF5I 330 GGATG GGATG(2/0)
BstYI 185 AGATCT [GA]^GATC[CT]
DdeI 182 CTAAG C^T[ACGT]AG
DpnII 186 GATC ^GATC
Fnu4HI 85 GCAGC GC^[ACGT]GC
FokI 330 GGATG GGATG(9/13)
ItaI 85 GCAGC GC^[ACGT]GC
MaeII 281 ACGT A^CGT
MaeIII 121 GTAAC ^GT[ACGT]AC
MboI 186 GATC ^GATC
MboII 205 GAAGA GAAGA(8/7)
MflI 185 AGATCT [GA]^GATC[CT]
MnlI 52 GAGG CCTC(7/6)
NciI 89 CCCGG CC^[GC]GG
NdeII 186 GATC ^GATC
NlaIV 19 GGAACC GG[ACGT]^[ACGT]CC
Sau3AI 186 GATC ^GATC
SfcI 221 CTACAG C^T[GA][CT]AG
SspI 364 AATATT AAT^ATT
TseI 85 GCAGC G^C[AT]GC
TspRI 39 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 285 CCAAAAATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 185 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcsI 78 GAATTT [GA]^AATT[CT]
208 GAATTT
AluI 29 AGCT AG^CT
197 AGCT
ApoI 78 GAATTT [GA]^AATT[CT]
208 GAATTT
BmyI 7 GAGCCC G[AGT]GC[ACT]^C
246 GAGCAC
BsiYI 10 CCCTTCTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
90 CCGGAACATGG
BslI 10 CCCTTCTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
90 CCGGAACATGG
BsmAI 304 GAGAC GTCTC(1/5)
34 GTCTC
Bsp1286I 7 GAGCCC G[AGT]GC[ACT]^C
246 GAGCAC
BsrI 69 ACTGG ACTGG(1/-1)
38 CCAGT
BstNI 23 CCTGG CC^[AT]GG
174 CCAGG
EcoRII 23 CCTGG ^CC[AT]GG
174 CCAGG
HapII 90 CCGG C^CGG
234 CCGG
HinfI 276 GATTC G^A[ACGT]TC
380 GAATC
HpaII 90 CCGG C^CGG
234 CCGG
MspI 90 CCGG C^CGG
234 CCGG
MvaI 23 CCTGG CC^[AT]GG
174 CCAGG
NlaIII 60 CATG CATG^
96 CATG
TfiI 276 GATTC G^A[AT]TC
380 GAATC
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# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
#########################################
BssKI ScrFI Tsp509I