Restriction map for
pFN21AE0250
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I FauI FbaI FseI
FspI HaeIII HgaI Hin1I HincII
HindII HinfI HpaI KasI KpnI
MaeI MamI MfeI MluI MluNI
MscI MseI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NspI NspV PacI PaeR7I
PflFI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccIII 575 TCCGGA T^CCGGA
AfaI 436 GTAC GT^AC
AgeI 98 ACCGGT A^CCGGT
BamHI 578 GGATCC G^GATCC
BcnI 105 CCGGG CC^[GC]GG
BglII 389 AGATCT A^GATCT
BmyI 107 GGGCAC G[AGT]GC[ACT]^C
BseAI 575 TCCGGA T^CCGGA
BsmFI 41 GGGAC GGGAC(10/14)
Bsp1286I 107 GGGCAC G[AGT]GC[ACT]^C
BspEI 575 TCCGGA T^CCGGA
BspMI 411 GCAGGT ACCTGC(4/8)
BsrI 111 ACTGG ACTGG(1/-1)
BstAPI 399 GCATGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DsaI 23 CCGCGG C^C[GA][CT]GG
EarI 448 GAAGAG CTCTTC(1/4)
EcoT22I 353 ATGCAT ATGCA^T
HaeII 165 GGCGCT [GA]GCGC^[CT]
HindIII 204 AAGCTT A^AGCTT
Ksp632I 448 GAAGAG CTCTTC(1/4)
KspI 23 CCGCGG CCGC^GG
MaeII 554 ACGT A^CGT
MaeIII 442 GTGAC ^GT[ACGT]AC
MroI 575 TCCGGA T^CCGGA
NciI 105 CCGGG CC^[GC]GG
NruI 378 TCGCGA TCG^CGA
NsiI 353 ATGCAT ATGCA^T
PflMI 225 CCAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 98 ACCGGT A^CCGGT
PstI 409 CTGCAG CTGCA^G
PvuII 454 CAGCTG CAG^CTG
RsaI 436 GTAC GT^AC
SacII 23 CCGCGG CCGC^GG
SapI 448 GAAGAGC GCTCTTC(1/4)
Tsp45I 442 GTGAC ^GT[GC]AC
Tsp509I 195 AATT ^AATT
TspRI 110 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 225 CCAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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BsaI 58 GAGACC GGTCTC(1/5)
188 GAGACC
BsaWI 98 ACCGGT [AT]^CCGG[AT]
575 TCCGGA
BsgI 91 CTGCAC GTGCAG(16/14)
481 GTGCAG
BsmAI 58 GAGAC GTCTC(1/5)
188 GAGAC
BsrFI 61 ACCGGC [GA]^CCGG[CT]
98 ACCGGT
BstF5I 573 CATCC GGATG(2/0)
504 GGATG
CfoI 9 GCGC GCG^C
166 GCGC
Cfr10I 61 ACCGGC [GA]^CCGG[CT]
98 ACCGGT
DdeI 136 CTGAG C^T[ACGT]AG
540 CTTAG
FokI 573 CATCC GGATG(9/13)
504 GGATG
HhaI 9 GCGC GCG^C
166 GCGC
HinP1I 9 GCGC G^CGC
166 GCGC
HphI 441 GGTGA GGTGA(8/7)
530 TCACC
MspA1I 23 CCGCGG C[AC]G^C[GT]G
454 CAGCTG
NlaIII 144 CATG CATG^
400 CATG
SfaNI 405 GATGC GCATC(5/9)
572 GCATC
SfcI 275 CTGTAG C^T[GA][CT]AG
409 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AccII AciI AluI AlwI BbvI
BpmI BsaJI BsiYI BslI BssKI
BstNI BstUI BstYI Cac8I DpnII
EcoRII Fnu4HI HapII HpaII ItaI
MboI MboII MflI MnlI MspI
MvaI MvnI MwoI NdeII NlaIV
Sau3AI ScrFI TseI XhoII