(Circular) (Six-base) MAPSORT of: pFN21AE0250.seq Check: 5061 from: 1 to: 5293 pFN21AE0250 5293 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3103 278 Size: 53 83 186 936 1567 2468 Fragments arranged by size: 2468 1567 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2636 1937 Size: 699 4594 Acc65I G'GTAC_C Cuts at: 2614 2614 Size: 5293 AclI AA'CG_TT Cuts at: 1563 3420 3793 1563 Size: 1857 373 3063 Fragments arranged by size: 3063 1857 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2609 3356 4740 5008 1632 Size: 977 747 1384 268 1917 Fragments arranged by size: 1917 1384 977 747 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5096 AflIII A'CryG_T Cuts at: 1203 4192 5169 1203 Size: 2989 977 1327 Fragments arranged by size: 2989 1327 977 AgeI A'CCGG_T Cuts at: 2103 4124 2103 Size: 2021 3272 AhdI GACnn_n'nnGTC Cuts at: 4022 4022 Size: 5293 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5293 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5293 AlwNI CAG_nnn'CTG Cuts at: 3036 4608 3036 Size: 1572 3721 ApaLI G'TGCA_C Cuts at: 3350 4506 3350 Size: 1156 4137 ApoI r'AATT_y Cuts at: 1493 2602 2828 2980 5089 1493 Size: 1109 226 152 2109 1697 Fragments arranged by size: 2109 1697 1109 226 152 AseI AT'TA_AT Cuts at: 160 1032 3847 160 Size: 872 2815 1606 Fragments arranged by size: 2815 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5293 AvaI C'yCGr_G Cuts at: 1870 1943 2618 1870 Size: 73 675 4545 Fragments arranged by size: 4545 675 73 BamHI G'GATC_C Cuts at: 2583 2623 2990 2583 Size: 40 367 4886 Fragments arranged by size: 4886 367 40 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2614 4069 618 Size: 325 217 627 827 1455 1842 Fragments arranged by size: 1842 1455 827 627 325 217 BanII G_rGCy'C Cuts at: 1391 2612 1391 Size: 1221 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4322 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3484 3518 1903 Size: 34 1547 34 3678 Fragments arranged by size: 3678 1547 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3185 4395 3185 Size: 1210 4083 BclI T'GATC_A Cuts at: 1548 1548 Size: 5293 BfrBI ATG'CAT Cuts at: 2360 2360 Size: 5293 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3904 136 Size: 107 122 71 631 605 2232 1525 Fragments arranged by size: 2232 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2394 5288 2394 Size: 2894 2399 BlpI GC'TnA_GC Cuts at: 2710 2710 Size: 5293 Bme1580I G_kGCm'C Cuts at: 1790 2116 3354 4510 1790 Size: 326 1238 1156 2573 Fragments arranged by size: 2573 1238 1156 326 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5293 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3024 3982 442 Size: 416 53 83 399 24 1607 958 1753 Fragments arranged by size: 1753 1607 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5293 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2383 2483 3953 1239 Size: 902 242 100 1470 2579 Fragments arranged by size: 2579 1470 902 242 100 Bpu10I CC'TnA_GC Cuts at: 2141 2141 Size: 5293 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3418 4283 4581 3418 Size: 865 298 4130 Fragments arranged by size: 4130 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2057 2187 3956 882 Size: 617 558 130 1769 2219 Fragments arranged by size: 2219 1769 617 558 130 BsaAI yAC'GTr Cuts at: 493 1204 3084 493 Size: 711 1880 2702 Fragments arranged by size: 2702 1880 711 BsaBI GATnn'nnATC Cuts at: 5287 5287 Size: 5293 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3100 3482 4117 275 Size: 53 83 186 936 1567 382 635 1451 Fragments arranged by size: 1567 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2103 2580 3726 4124 4398 4545 4956 2103 Size: 477 1146 398 274 147 411 2440 Fragments arranged by size: 2440 1146 477 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5068 Fragments arranged by size: 5068 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5293 BseYI C'CCAG_C Cuts at: 4496 4992 4496 Size: 496 4797 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 2507 1163 Size: 573 345 426 3949 Fragments arranged by size: 3949 573 426 345 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2743 3504 4532 1540 Size: 275 186 742 761 1028 2301 Fragments arranged by size: 2301 1028 761 742 275 186 BsiHKAI G_wGCw'C Cuts at: 3354 3439 4510 3354 Size: 85 1071 4137 Fragments arranged by size: 4137 1071 85 BsmI GAATG_Cn' Cuts at: 2804 2897 2804 Size: 93 5200 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2116 2612 3354 3439 4510 1391 Size: 399 326 496 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 496 399 326 85 BspEI T'CCGG_A Cuts at: 2580 4956 2580 Size: 2376 2917 BspHI T'CATG_A Cuts at: 3182 4912 3182 Size: 1730 3563 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2407 2648 844 Size: 1563 241 3489 Fragments arranged by size: 3489 1563 241 BsrBI CCG'CTC Cuts at: 1394 1823 3180 1394 Size: 429 1357 3507 Fragments arranged by size: 3507 1357 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3788 3962 65 Size: 1183 2540 174 1396 Fragments arranged by size: 2540 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2066 2103 3937 4124 1700 Size: 110 256 37 1834 187 2869 Fragments arranged by size: 2869 1834 256 187 110 37 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5293 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5293 BssSI C'ACGA_G Cuts at: 3353 4365 3353 Size: 1012 4281 BstAPI GCAn_nnn'nTGC Cuts at: 794 2410 794 Size: 1616 3677 BstBI TT'CG_AA Cuts at: 4097 4097 Size: 5293 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5293 BstYI r'GATC_y Cuts at: 1972 2152 2394 2583 2623 2990 3375 3392 4844 Size: 180 242 189 40 367 385 17 1452 Cuts at: 4844 4930 4942 5288 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 242 189 180 86 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 513 Size: 552 939 24 3778 Fragments arranged by size: 3778 939 552 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2400 2455 487 Size: 683 56 1174 55 3325 Fragments arranged by size: 3325 1174 683 56 55 BtsI GCAGTG_nn' Cuts at: 2809 3603 3623 2809 Size: 794 20 4479 Fragments arranged by size: 4479 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5293 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5258 DraI TTT'AAA Cuts at: 2597 2597 Size: 5293 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4300 809 Size: 507 2984 1802 Fragments arranged by size: 2984 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2740 3629 5258 Size: 54 1425 50 275 18 910 889 1629 Cuts at: 5258 8 Size: 43 Fragments arranged by size: 1629 1425 910 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2740 1537 Size: 275 928 4090 Fragments arranged by size: 4090 928 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2448 3223 1353 Size: 1095 775 3423 Fragments arranged by size: 3423 1095 775 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2081 3880 4254 4400 387 Size: 1064 138 492 1799 374 146 1280 Fragments arranged by size: 1799 1280 1064 492 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2383 2483 2609 3356 3953 4740 Size: 393 509 242 100 126 747 597 787 Cuts at: 4740 5008 1239 Size: 268 1524 Fragments arranged by size: 1524 787 747 597 509 393 268 242 126 100 EcoRI G'AATT_C Cuts at: 2602 2980 2602 Size: 378 4915 FspI TGC'GCA Cuts at: 3799 3799 Size: 5293 HaeII r_GCGC'y Cuts at: 1678 2174 4440 1678 Size: 496 2266 2531 Fragments arranged by size: 2531 2266 496 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3934 3966 4008 4040 1373 Size: 32 135 32 2362 32 42 32 2626 Fragments arranged by size: 2626 2362 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2637 2883 669 Size: 988 281 699 246 3079 Fragments arranged by size: 3079 988 699 281 246 HindIII A'AGCT_T Cuts at: 748 2209 748 Size: 1461 3832 HpaI GTT'AAC Cuts at: 2883 2883 Size: 5293 KpnI G_GTAC'C Cuts at: 2618 2618 Size: 5293 MfeI C'AATT_G Cuts at: 2892 2892 Size: 5293 MluI A'CGCG_T Cuts at: 5169 5169 Size: 5293 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4407 4591 1305 Size: 3102 184 2007 Fragments arranged by size: 3102 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3471 Fragments arranged by size: 3471 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3251 3610 3769 518 Size: 648 2085 359 159 2042 Fragments arranged by size: 2085 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 2461 2709 3386 4534 4779 1606 Size: 424 431 248 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 431 424 248 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3802 Fragments arranged by size: 3802 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5293 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5293 NotI GC'GGCC_GC Cuts at: 2740 2740 Size: 5293 NruI TCG'CGA Cuts at: 2385 2385 Size: 5293 NsiI A_TGCA'T Cuts at: 2362 2362 Size: 5293 NspI r_CATG'y Cuts at: 1132 1229 2651 4196 1132 Size: 97 1422 1545 2229 Fragments arranged by size: 2229 1545 1422 97 PasI CC'CwG_GG Cuts at: 1348 2188 1348 Size: 840 4453 PciI A'CATG_T Cuts at: 4192 4192 Size: 5293 PflMI CCAn_nnn'nTGG Cuts at: 1108 2236 1108 Size: 1128 4165 PmeI GTTT'AAAC Cuts at: 2597 2597 Size: 5293 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3362 3394 4875 4907 3362 Size: 32 1481 32 3748 Fragments arranged by size: 3748 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5293 PsiI TTA'TAA Cuts at: 2863 2863 Size: 5293 PstI C_TGCA'G Cuts at: 830 2418 2645 830 Size: 1588 227 3478 Fragments arranged by size: 3478 1588 227 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5293 PvuII CAG'CTG Cuts at: 2461 2461 Size: 5293 SacII CC_GC'GG Cuts at: 2031 2031 Size: 5293 SalI G'TCGA_C Cuts at: 1936 2635 1936 Size: 699 4594 SapI GCTCTTCn'nnn_ Cuts at: 2448 2448 Size: 5293 SbfI CC_TGCA'GG Cuts at: 2645 2645 Size: 5293 ScaI AGT'ACT Cuts at: 3541 5253 3541 Size: 1712 3581 SfcI C'TryA_G Cuts at: 826 1045 2280 2414 2641 3776 4457 4648 826 Size: 219 1235 134 227 1135 681 191 1471 Fragments arranged by size: 1471 1235 1135 681 227 219 191 134 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5293 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5293 SmaI CCC'GGG Cuts at: 1872 2620 1872 Size: 748 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3397 4298 4560 820 Size: 197 926 1454 901 262 1553 Fragments arranged by size: 1553 1454 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3084 493 Size: 2591 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5293 SphI G_CATG'C Cuts at: 1132 2651 1132 Size: 1519 3774 SspI AAT'ATT Cuts at: 5 52 3217 5 Size: 47 3165 2081 Fragments arranged by size: 3165 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5293 StyI C'CwwG_G Cuts at: 513 1065 2004 2732 513 Size: 552 939 728 3074 Fragments arranged by size: 3074 939 728 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2211 3320 3337 3490 3675 4120 325 Size: 1255 631 1109 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1109 631 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3539 5251 96 Size: 274 80 33 51 1443 1562 1712 138 Fragments arranged by size: 1712 1562 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5253 5285 5253 Size: 32 5261 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5293 XbaI T'CTAG_A Cuts at: 2629 2629 Size: 5293 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5293 XmaI C'CCGG_G Cuts at: 1870 2618 1870 Size: 748 4545 XmnI GAAnn'nnTTC Cuts at: 3422 3422 Size: 5293 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3101 276 Size: 53 83 186 936 1567 2468 Fragments arranged by size: 2468 1567 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI