(Linear) (Six-base) MAPSORT of: KIEE0250.seq  Check: 4233  from: 1  to: 588



KIEE0250.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AgeI A'CCGG_T

Cuts at:      0      98     588 
   Size:         98     490
 
BamHI G'GATC_C

Cuts at:      0     578     588 
   Size:        578      10
 
BfrBI ATG'CAT

Cuts at:      0     355     588 
   Size:        355     233
 
BglII A'GATC_T

Cuts at:      0     389     588 
   Size:        389     199
 
Bme1580I G_kGCm'C

Cuts at:      0     111     588 
   Size:        111     477
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     378     478     588 
   Size:        136     242     100     110

  Fragments arranged by size: 

                 242     136     110     100
 
Bpu10I CC'TnA_GC

Cuts at:      0     136     588 
   Size:        136     452
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      52     182     588 
   Size:         52     130     406

  Fragments arranged by size: 

                 406     130      52
 
BsaWI w'CCGG_w

Cuts at:      0      98     575     588 
   Size:         98     477      13

  Fragments arranged by size: 

                 477      98      13
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     502     588 
   Size:         76     426      86

  Fragments arranged by size: 

                 426      86      76
 
Bsp1286I G_dGCh'C

Cuts at:      0     111     588 
   Size:        111     477
 
BspEI T'CCGG_A

Cuts at:      0     575     588 
   Size:        575      13
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     402     588 
   Size:        402     186
 
BsrFI r'CCGG_y

Cuts at:      0      61      98     588 
   Size:         61      37     490

  Fragments arranged by size: 

                 490      61      37
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     405     588 
   Size:        405     183
 
BstYI r'GATC_y

Cuts at:      0     147     389     578     588 
   Size:        147     242     189      10

  Fragments arranged by size: 

                 242     189     147      10
 
BtgI C'CryG_G

Cuts at:      0      23     588 
   Size:         23     565
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     395     450     588 
   Size:        395      55     138

  Fragments arranged by size: 

                 395     138      55
 
EarI CTCTTCn'nnn_

Cuts at:      0     443     588 
   Size:        443     145
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      76     588 
   Size:         76     512
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     378     478     588 
   Size:        136     242     100     110

  Fragments arranged by size: 

                 242     136     110     100
 
HaeII r_GCGC'y

Cuts at:      0     169     588 
   Size:        169     419
 
HindIII A'AGCT_T

Cuts at:      0     204     588 
   Size:        204     384
 
MspA1I CmG'CkG

Cuts at:      0      25     456     588 
   Size:         25     431     132

  Fragments arranged by size: 

                 431     132      25
 
NruI TCG'CGA

Cuts at:      0     380     588 
   Size:        380     208
 
NsiI A_TGCA'T

Cuts at:      0     357     588 
   Size:        357     231
 
PasI CC'CwG_GG

Cuts at:      0     183     588 
   Size:        183     405
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     231     588 
   Size:        231     357
 
PstI C_TGCA'G

Cuts at:      0     413     588 
   Size:        413     175
 
PvuII CAG'CTG

Cuts at:      0     456     588 
   Size:        456     132
 
SacII CC_GC'GG

Cuts at:      0      26     588 
   Size:         26     562
 
SapI GCTCTTCn'nnn_

Cuts at:      0     443     588 
   Size:        443     145
 
SfcI C'TryA_G

Cuts at:      0     275     409     588 
   Size:        275     134     179

  Fragments arranged by size: 

                 275     179     134
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     206     588 
   Size:        206     382
 

 Enzymes that do cut:

     AgeI    BamHI    BfrBI    BglII Bme1580I     BpmI   Bpu10I     BsaI
    BsaWI     BsgI Bsp1286I    BspEI    BspMI    BsrFI   BstAPI    BstYI
     BtgI    BtgZI     EarI     EciI  Eco57MI    HaeII  HindIII   MspA1I
     NruI     NsiI     PasI    PflMI     PstI    PvuII    SacII     SapI
     SfcI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI
     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NspI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI