Restriction map for
pFN21AE0248
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BsgI BsiEI BsiWI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DdeI
DpnII DraI DraII DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HinfI HpaI
HphI KasI KpnI KspI MaeIII
MamI MboI MfeI MflI MluI
MluNI MscI MseI MslI MunI
MvnI NaeI NarI NcoI NdeI
NdeII NgoMIV NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp45I Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 112 GGCGTC G[GA]^CG[CT]C
AfaI 327 GTAC GT^AC
AflIII 164 ACGTGT A^C[GA][CT]GT
AlwNI 10 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 363 GGACC G^G[AT]CC
BanII 58 GAGCTC G[GA]GC[CT]^C
BbrPI 163 CACGTG CAC^GTG
BcnI 109 CCCGG CC^[GC]GG
BfaI 205 CTAG C^TAG
BsaAI 163 CACGTG [CT]AC^GT[GA]
BsaHI 112 GGCGTC G[GA]^CG[CT]C
BsaI 76 GAGACC GGTCTC(1/5)
BsmBI 114 CGTCTC CGTCTC(1/5)
BsrI 288 ACTGG ACTGG(1/-1)
BstNI 255 CCTGG CC^[AT]GG
Cfr13I 363 GGACC G^G[ACGT]CC
DrdI 106 GACCCCGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 17 TGGCCG [CT]^GGCC[GA]
EcoRII 255 CCTGG ^CC[AT]GG
HaeIII 18 GGCC GG^CC
Hin1I 112 GGCGTC G[GA]^CG[CT]C
MaeI 205 CTAG C^TAG
MspA1I 13 CAGCTG C[AC]G^C[GT]G
MvaI 255 CCTGG CC^[AT]GG
NciI 109 CCCGG CC^[GC]GG
NheI 204 GCTAGC G^CTAGC
NlaIV 351 GGAGCC GG[ACGT]^[ACGT]CC
PflMI 9 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 163 CACGTG CAC^GTG
PmlI 163 CACGTG CAC^GTG
PvuII 13 CAGCTG CAG^CTG
RsaI 327 GTAC GT^AC
SacI 58 GAGCTC GAGCT^C
Sau96I 363 GGACC G^G[ACGT]CC
TaqI 56 TCGA T^CGA
Tsp509I 157 AATT ^AATT
TspRI 330 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 56 TCGA T^CGA
Van91I 9 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccIII 135 TCCGGA T^CCGGA
297 TCCGGA
AspHI 39 GTGCTC G[AT]GC[AT]^C
58 GAGCTC
BbsI 237 GAAGAC GAAGAC(2/6)
265 GTCTTC
BmyI 39 GTGCTC G[AGT]GC[ACT]^C
58 GAGCTC
BpuAI 237 GAAGAC GAAGAC(2/6)
265 GTCTTC
BsaJI 71 CCGAGG C^C[ACGT][ACGT]GG
108 CCCCGG
BsaWI 135 TCCGGA [AT]^CCGG[AT]
297 TCCGGA
BseAI 135 TCCGGA T^CCGGA
297 TCCGGA
BsiHKAI 39 GTGCTC G[AT]GC[AT]^C
58 GAGCTC
BsmAI 76 GAGAC GTCTC(1/5)
115 GTCTC
Bsp1286I 39 GTGCTC G[AGT]GC[ACT]^C
58 GAGCTC
BspEI 135 TCCGGA T^CCGGA
297 TCCGGA
BssKI 109 CCCGG ^CC[ACGT]GG
255 CCTGG
BstF5I 89 GGATG GGATG(2/0)
300 GGATG
EarI 322 GAAGAG CTCTTC(1/4)
346 GAAGAG
FokI 89 GGATG GGATG(9/13)
300 GGATG
HgaI 240 GACGC GACGC(5/10)
113 GCGTC
Ksp632I 322 GAAGAG CTCTTC(1/4)
346 GAAGAG
MroI 135 TCCGGA T^CCGGA
297 TCCGGA
ScrFI 109 CCCGG CC^[ACGT]GG
255 CCTGG
SfaNI 301 GATGC GCATC(5/9)
313 GATGC
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# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AciI AluI BbvI BseRI BsiYI
BslI Cac8I Fnu4HI HapII HpaII
ItaI MaeII MboII MnlI MspI
MwoI TseI