(Circular) (Six-base) MAPSORT of: pFN21AE0247.seq Check: 3113 from: 1 to: 6556 pFN21AE0247 6556 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3488 3488 Size: 6556 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4366 278 Size: 53 83 186 936 2830 2468 Fragments arranged by size: 2830 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3899 1937 Size: 1962 4594 Acc65I G'GTAC_C Cuts at: 3877 3877 Size: 6556 AclI AA'CG_TT Cuts at: 1563 4683 5056 1563 Size: 3120 373 3063 Fragments arranged by size: 3120 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2007 2037 2094 2163 2622 2697 3099 3150 Size: 375 30 57 69 459 75 402 51 Cuts at: 3150 3325 4619 6003 6271 1632 Size: 175 1294 1384 268 1917 Fragments arranged by size: 1917 1384 1294 459 402 375 268 175 75 69 57 51 30 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6359 AflIII A'CryG_T Cuts at: 1203 5455 6432 1203 Size: 4252 977 1327 Fragments arranged by size: 4252 1327 977 AgeI A'CCGG_T Cuts at: 5387 5387 Size: 6556 AhdI GACnn_n'nnGTC Cuts at: 5285 5285 Size: 6556 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6556 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6556 AlwNI CAG_nnn'CTG Cuts at: 2479 2727 4299 5871 2479 Size: 248 1572 1572 3164 Fragments arranged by size: 3164 1572 1572 248 ApaLI G'TGCA_C Cuts at: 4613 5769 4613 Size: 1156 5400 ApoI r'AATT_y Cuts at: 1493 2186 2299 2369 2734 2901 3477 3592 3865 Size: 693 113 70 365 167 576 115 273 Cuts at: 3865 4091 4243 6352 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 693 576 365 273 226 167 152 115 113 70 AseI AT'TA_AT Cuts at: 160 1032 5110 160 Size: 872 4078 1606 Fragments arranged by size: 4078 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6556 AvaI C'yCGr_G Cuts at: 1870 1943 3881 1870 Size: 73 1938 4545 Fragments arranged by size: 4545 1938 73 BamHI G'GATC_C Cuts at: 2680 3886 4253 2680 Size: 1206 367 4983 Fragments arranged by size: 4983 1206 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3877 5332 618 Size: 325 217 627 2090 1455 1842 Fragments arranged by size: 2090 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2667 3875 1391 Size: 1276 1208 4072 Fragments arranged by size: 4072 1276 1208 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5585 BbvCI CC'TCA_GC Cuts at: 2251 2499 3162 3433 2251 Size: 248 663 271 5374 Fragments arranged by size: 5374 663 271 248 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4747 4781 1903 Size: 34 2810 34 3678 Fragments arranged by size: 3678 2810 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4448 5658 4448 Size: 1210 5346 BclI T'GATC_A Cuts at: 1548 2239 1548 Size: 691 5865 BfrBI ATG'CAT Cuts at: 2425 2425 Size: 6556 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5167 136 Size: 107 122 71 631 605 3495 1525 Fragments arranged by size: 3495 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2230 6551 2230 Size: 4321 2235 BlpI GC'TnA_GC Cuts at: 3973 3973 Size: 6556 Bme1580I G_kGCm'C Cuts at: 1790 4617 5773 1790 Size: 2827 1156 2573 Fragments arranged by size: 2827 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6556 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2124 4287 5245 Size: 416 53 83 399 24 707 2163 958 Cuts at: 5245 442 Size: 1753 Fragments arranged by size: 2163 1753 958 707 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6556 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2109 2514 2109 Size: 405 6151 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2354 5216 1239 Size: 1115 2862 2579 Fragments arranged by size: 2862 2579 1115 Bpu10I CC'TnA_GC Cuts at: 2251 2499 3162 3433 2251 Size: 248 663 271 5374 Fragments arranged by size: 5374 663 271 248 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2612 4681 5546 5844 2612 Size: 2069 865 298 3324 Fragments arranged by size: 3324 2069 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5219 882 Size: 617 3720 2219 Fragments arranged by size: 3720 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4347 493 Size: 711 3143 2702 Fragments arranged by size: 3143 2702 711 BsaBI GATnn'nnATC Cuts at: 2460 6550 2460 Size: 4090 2466 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4363 4745 5380 275 Size: 53 83 186 936 2830 382 635 1451 Fragments arranged by size: 2830 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4989 5387 5661 5808 6219 4989 Size: 398 274 147 411 5326 Fragments arranged by size: 5326 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3337 3367 1178 Size: 30 165 30 1934 30 4367 Fragments arranged by size: 4367 1934 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2983 3518 1186 Size: 1797 535 4224 Fragments arranged by size: 4224 1797 535 BseYI C'CCAG_C Cuts at: 5759 6255 5759 Size: 496 6060 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3445 1163 Size: 573 1709 4274 Fragments arranged by size: 4274 1709 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4006 4767 5795 1540 Size: 275 186 2005 761 1028 2301 Fragments arranged by size: 2301 2005 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4617 4702 5773 4617 Size: 85 1071 5400 Fragments arranged by size: 5400 1071 85 BsmI GAATG_Cn' Cuts at: 2723 2815 3741 3808 4067 4160 2723 Size: 92 926 67 259 93 5119 Fragments arranged by size: 5119 926 259 93 92 67 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2667 3875 4617 4702 5773 1391 Size: 399 877 1208 742 85 1071 2174 Fragments arranged by size: 2174 1208 1071 877 742 399 85 BspEI T'CCGG_A Cuts at: 6219 6219 Size: 6556 BspHI T'CATG_A Cuts at: 3082 4445 6175 3082 Size: 1363 1730 3463 Fragments arranged by size: 3463 1730 1363 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2315 3488 3911 844 Size: 1471 1173 423 3489 Fragments arranged by size: 3489 1471 1173 423 BsrBI CCG'CTC Cuts at: 1394 1823 4443 1394 Size: 429 2620 3507 Fragments arranged by size: 3507 2620 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5051 5225 65 Size: 1183 3803 174 1396 Fragments arranged by size: 3803 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5200 5387 1700 Size: 110 3390 187 2869 Fragments arranged by size: 3390 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6556 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6556 BssSI C'ACGA_G Cuts at: 4616 5628 4616 Size: 1012 5544 BstAPI GCAn_nnn'nTGC Cuts at: 794 2261 2727 3812 794 Size: 1467 466 1085 3538 Fragments arranged by size: 3538 1467 1085 466 BstBI TT'CG_AA Cuts at: 5360 5360 Size: 6556 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6556 BstYI r'GATC_y Cuts at: 1972 2230 2680 2839 3886 4253 4638 4655 6107 Size: 258 450 159 1047 367 385 17 1452 Cuts at: 6107 6193 6205 6551 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1047 450 385 367 346 258 159 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3466 3466 Size: 6556 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6004 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5817 Fragments arranged by size: 5817 683 56 BtsI GCAGTG_nn' Cuts at: 2728 3457 4072 4866 4886 2728 Size: 729 615 794 20 4398 Fragments arranged by size: 4398 794 729 615 20 ClaI AT'CG_AT Cuts at: 1554 2757 1554 Size: 1203 5353 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3715 3750 529 Size: 35 3151 35 3335 Fragments arranged by size: 3335 3151 35 35 DraI TTT'AAA Cuts at: 3860 3860 Size: 6556 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5563 809 Size: 507 4247 1802 Fragments arranged by size: 4247 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2983 4003 4892 Size: 54 1425 50 275 18 1153 1020 889 Cuts at: 4892 6521 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1153 1020 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4003 1537 Size: 275 2191 4090 Fragments arranged by size: 4090 2191 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3310 4486 1353 Size: 1957 1176 3423 Fragments arranged by size: 3423 1957 1176 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5143 5517 5663 387 Size: 1064 138 3554 374 146 1280 Fragments arranged by size: 3554 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2007 2037 2094 2163 2354 2622 2697 Size: 393 375 30 57 69 191 268 75 Cuts at: 2697 3099 3150 3325 4619 5216 6003 6271 1239 Size: 402 51 175 1294 597 787 268 1524 Fragments arranged by size: 1524 1294 787 597 402 393 375 268 268 191 175 75 69 57 51 30 EcoO109I rG'GnC_Cy Cuts at: 2362 2587 2362 Size: 225 6331 EcoRI G'AATT_C Cuts at: 2186 2299 3592 3865 4243 2186 Size: 113 1293 273 378 4499 Fragments arranged by size: 4499 1293 378 273 113 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2234 2588 2234 Size: 354 6202 FspI TGC'GCA Cuts at: 5062 5062 Size: 6556 HaeII r_GCGC'y Cuts at: 1678 5703 1678 Size: 4025 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3347 3379 3612 3644 5197 Size: 32 135 32 1775 32 233 32 1553 Cuts at: 5197 5229 5271 5303 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1775 1553 233 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3366 3900 4146 669 Size: 988 281 1428 534 246 3079 Fragments arranged by size: 3079 1428 988 534 281 246 HindIII A'AGCT_T Cuts at: 748 2550 748 Size: 1802 4754 HpaI GTT'AAC Cuts at: 4146 4146 Size: 6556 KpnI G_GTAC'C Cuts at: 3881 3881 Size: 6556 MfeI C'AATT_G Cuts at: 4155 4155 Size: 6556 MluI A'CGCG_T Cuts at: 6432 6432 Size: 6556 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2291 5670 5854 1305 Size: 986 3379 184 2007 Fragments arranged by size: 3379 2007 986 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2985 10 Size: 54 1425 343 1153 3581 Fragments arranged by size: 3581 1425 1153 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2881 4514 4873 5032 518 Size: 648 1715 1633 359 159 2042 Fragments arranged by size: 2042 1715 1633 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2183 3972 4649 5797 6042 1606 Size: 577 1789 677 1148 245 2120 Fragments arranged by size: 2120 1789 1148 677 577 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6004 NdeI CA'TA_TG Cuts at: 387 387 Size: 6556 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6556 NotI GC'GGCC_GC Cuts at: 4003 4003 Size: 6556 NsiI A_TGCA'T Cuts at: 2427 2427 Size: 6556 NspI r_CATG'y Cuts at: 1132 1229 3914 5459 1132 Size: 97 2685 1545 2229 Fragments arranged by size: 2685 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2359 1348 Size: 1011 5545 PciI A'CATG_T Cuts at: 5455 5455 Size: 6556 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6556 PmeI GTTT'AAAC Cuts at: 3860 3860 Size: 6556 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4625 4657 6138 6170 4625 Size: 32 1481 32 5011 Fragments arranged by size: 5011 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2587 2587 Size: 6556 PshAI GACnn'nnGTC Cuts at: 1613 2277 3576 1613 Size: 664 1299 4593 Fragments arranged by size: 4593 1299 664 PsiI TTA'TAA Cuts at: 4126 4126 Size: 6556 PstI C_TGCA'G Cuts at: 830 3908 830 Size: 3078 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6556 PvuII CAG'CTG Cuts at: 2183 2183 Size: 6556 SalI G'TCGA_C Cuts at: 1936 3898 1936 Size: 1962 4594 SbfI CC_TGCA'GG Cuts at: 3908 3908 Size: 6556 ScaI AGT'ACT Cuts at: 4804 6516 4804 Size: 1712 4844 SfcI C'TryA_G Cuts at: 826 1045 3324 3852 3904 5039 5720 5911 826 Size: 219 2279 528 52 1135 681 191 1471 Fragments arranged by size: 2279 1471 1135 681 528 219 191 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6556 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6556 SmaI CCC'GGG Cuts at: 1872 3883 1872 Size: 2011 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2591 4660 5561 5823 820 Size: 197 926 648 2069 901 262 1553 Fragments arranged by size: 2069 1553 926 901 648 262 197 SnaBI TAC'GTA Cuts at: 493 4347 493 Size: 3854 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6556 SphI G_CATG'C Cuts at: 1132 3914 1132 Size: 2782 3774 SspI AAT'ATT Cuts at: 5 52 4480 5 Size: 47 4428 2081 Fragments arranged by size: 4428 2081 47 StuI AGG'CCT Cuts at: 1517 3610 1517 Size: 2093 4463 StyI C'CwwG_G Cuts at: 513 1065 3995 513 Size: 552 2930 3074 Fragments arranged by size: 3074 2930 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2372 3499 4583 4600 4753 4938 5383 Size: 1255 792 1127 1084 17 153 185 445 Cuts at: 5383 325 Size: 1498 Fragments arranged by size: 1498 1255 1127 1084 792 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4802 6514 96 Size: 274 80 33 51 1443 2825 1712 138 Fragments arranged by size: 2825 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3337 3369 6516 6548 3337 Size: 32 3147 32 3345 Fragments arranged by size: 3345 3147 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2061 1316 Size: 745 5811 XbaI T'CTAG_A Cuts at: 3892 3892 Size: 6556 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3559 3559 Size: 6556 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6556 XmaI C'CCGG_G Cuts at: 1870 3881 1870 Size: 2011 4545 XmnI GAAnn'nnTTC Cuts at: 4685 4685 Size: 6556 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4364 276 Size: 53 83 186 936 2830 2468 Fragments arranged by size: 2830 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI