Restriction map for
pFN21AE0247
(Linear) (Six-base) MAPSORT of: KIEE0247.seq Check: 9679 from: 1 to: 1851
KIEE0247.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1483 1851
Size: 1483 368
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 32 89 158 617 692 1094 1145
Size: 2 30 57 69 459 75 402 51
Cuts at: 1145 1320 1851
Size: 175 531
Fragments arranged by size:
531 459 402 175 75 69 57 51
30 2
AlwNI CAG_nnn'CTG
Cuts at: 0 474 722 1851
Size: 474 248 1129
Fragments arranged by size:
1129 474 248
ApoI r'AATT_y
Cuts at: 0 181 294 364 729 896 1472 1587 1851
Size: 181 113 70 365 167 576 115 264
Fragments arranged by size:
576 365 264 181 167 115 113 70
BamHI G'GATC_C
Cuts at: 0 675 1851
Size: 675 1176
BanII G_rGCy'C
Cuts at: 0 662 1851
Size: 662 1189
BbvCI CC'TCA_GC
Cuts at: 0 246 494 1157 1428 1851
Size: 246 248 663 271 423
Fragments arranged by size:
663 423 271 248 246
BclI T'GATC_A
Cuts at: 0 234 1851
Size: 234 1617
BfrBI ATG'CAT
Cuts at: 0 420 1851
Size: 420 1431
BglII A'GATC_T
Cuts at: 0 225 1851
Size: 225 1626
BmrI ACTGGGnnnn_n'
Cuts at: 0 119 1851
Size: 119 1732
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 104 509 1851
Size: 104 405 1342
Fragments arranged by size:
1342 405 104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 1851
Size: 349 1502
Bpu10I CC'TnA_GC
Cuts at: 0 246 494 1157 1428 1851
Size: 246 248 663 271 423
Fragments arranged by size:
663 423 271 248 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1851
Size: 607 1244
BsaBI GATnn'nnATC
Cuts at: 0 455 1851
Size: 455 1396
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1332 1362 1851
Size: 1332 30 489
Fragments arranged by size:
1332 489 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 978 1513 1851
Size: 978 535 338
Fragments arranged by size:
978 535 338
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1440 1851
Size: 1440 411
BsmI GAATG_Cn'
Cuts at: 0 718 810 1736 1803 1851
Size: 718 92 926 67 48
Fragments arranged by size:
926 718 92 67 48
Bsp1286I G_dGCh'C
Cuts at: 0 662 1851
Size: 662 1189
BspHI T'CATG_A
Cuts at: 0 1077 1851
Size: 1077 774
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 310 1483 1851
Size: 310 1173 368
Fragments arranged by size:
1173 368 310
BstAPI GCAn_nnn'nTGC
Cuts at: 0 256 722 1807 1851
Size: 256 466 1085 44
Fragments arranged by size:
1085 466 256 44
BstYI r'GATC_y
Cuts at: 0 225 675 834 1851
Size: 225 450 159 1017
Fragments arranged by size:
1017 450 225 159
Bsu36I CC'TnA_GG
Cuts at: 0 1461 1851
Size: 1461 390
BtsI GCAGTG_nn'
Cuts at: 0 723 1452 1851
Size: 723 729 399
Fragments arranged by size:
729 723 399
ClaI AT'CG_AT
Cuts at: 0 752 1851
Size: 752 1099
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1710 1745 1851
Size: 1710 35 106
Fragments arranged by size:
1710 106 35
EaeI y'GGCC_r
Cuts at: 0 978 1851
Size: 978 873
EarI CTCTTCn'nnn_
Cuts at: 0 1305 1851
Size: 1305 546
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 32 89 158 349 617 692 1094
Size: 2 30 57 69 191 268 75 402
Cuts at: 1094 1145 1320 1851
Size: 51 175 531
Fragments arranged by size:
531 402 268 191 175 75 69 57
51 30 2
EcoO109I rG'GnC_Cy
Cuts at: 0 357 582 1851
Size: 357 225 1269
Fragments arranged by size:
1269 357 225
EcoRI G'AATT_C
Cuts at: 0 181 294 1587 1851
Size: 181 113 1293 264
Fragments arranged by size:
1293 264 181 113
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 229 583 1851
Size: 229 354 1268
Fragments arranged by size:
1268 354 229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1342 1374 1607 1639 1851
Size: 1342 32 233 32 212
Fragments arranged by size:
1342 233 212 32 32
HincII GTy'rAC
Cuts at: 0 1361 1851
Size: 1361 490
HindIII A'AGCT_T
Cuts at: 0 545 1851
Size: 545 1306
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 1851
Size: 286 1565
MscI TGG'CCA
Cuts at: 0 980 1851
Size: 980 871
MslI CAynn'nnrTG
Cuts at: 0 876 1851
Size: 876 975
MspA1I CmG'CkG
Cuts at: 0 178 1851
Size: 178 1673
NsiI A_TGCA'T
Cuts at: 0 422 1851
Size: 422 1429
PasI CC'CwG_GG
Cuts at: 0 354 1851
Size: 354 1497
PpuMI rG'GwC_Cy
Cuts at: 0 582 1851
Size: 582 1269
PshAI GACnn'nnGTC
Cuts at: 0 272 1571 1851
Size: 272 1299 280
Fragments arranged by size:
1299 280 272
PvuII CAG'CTG
Cuts at: 0 178 1851
Size: 178 1673
SfcI C'TryA_G
Cuts at: 0 1319 1851
Size: 1319 532
SmlI C'TyrA_G
Cuts at: 0 586 1851
Size: 586 1265
StuI AGG'CCT
Cuts at: 0 1605 1851
Size: 1605 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 367 1494 1851
Size: 367 1127 357
Fragments arranged by size:
1127 367 357
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1332 1364 1851
Size: 1332 32 487
Fragments arranged by size:
1332 487 32
Tth111I GACn'n_nGTC
Cuts at: 0 56 1851
Size: 56 1795
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1554 1851
Size: 1554 297
Enzymes that do cut:
AarI AcuI AlwNI ApoI BamHI BanII BbvCI BclI
BfrBI BglII BmrI BplI BpmI Bpu10I BpuEI BsaBI
BsaXI BseRI BsgI BsmI Bsp1286I BspHI BspMI BstAPI
BstYI Bsu36I BtsI ClaI CspCI EaeI EarI Eco57MI
EcoO109I EcoRI FalI Hin4I HincII HindIII MmeI MscI
MslI MspA1I NsiI PasI PpuMI PshAI PvuII SfcI
SmlI StuI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbsI BcgI
BciVI BglI BlpI Bme1580I BmgBI BmtI BsaI BsaAI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmBI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I BtgI BtgZI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI
TatI XbaI XhoI XmaI XmnI ZraI