(Circular) (Six-base) MAPSORT of: pFN21AE0241.seq Check: 3386 from: 1 to: 5986 pFN21AE0241 5986 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3796 278 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3329 1937 Size: 1392 4594 Acc65I G'GTAC_C Cuts at: 2205 3307 2205 Size: 1102 4884 AclI AA'CG_TT Cuts at: 1563 4113 4486 1563 Size: 2550 373 3063 Fragments arranged by size: 3063 2550 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2028 2572 2739 4049 5433 5701 1632 Size: 396 544 167 1310 1384 268 1917 Fragments arranged by size: 1917 1384 1310 544 396 268 167 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5789 AflIII A'CryG_T Cuts at: 1203 2228 4885 5862 1203 Size: 1025 2657 977 1327 Fragments arranged by size: 2657 1327 1025 977 AgeI A'CCGG_T Cuts at: 4817 4817 Size: 5986 AhdI GACnn_n'nnGTC Cuts at: 4715 4715 Size: 5986 AleI CACnn'nnGTG Cuts at: 1166 2231 1166 Size: 1065 4921 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5986 AlwNI CAG_nnn'CTG Cuts at: 3729 5301 3729 Size: 1572 4414 ApaLI G'TGCA_C Cuts at: 4043 5199 4043 Size: 1156 4830 ApoI r'AATT_y Cuts at: 1493 3118 3295 3521 3673 5782 1493 Size: 1625 177 226 152 2109 1697 Fragments arranged by size: 2109 1697 1625 226 177 152 AseI AT'TA_AT Cuts at: 160 1032 4540 160 Size: 872 3508 1606 Fragments arranged by size: 3508 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 AvaI C'yCGr_G Cuts at: 1870 1943 3311 1870 Size: 73 1368 4545 Fragments arranged by size: 4545 1368 73 BamHI G'GATC_C Cuts at: 3316 3683 3316 Size: 367 5619 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2205 3307 4762 618 Size: 325 217 627 418 1102 1455 1842 Fragments arranged by size: 1842 1455 1102 627 418 325 217 BanII G_rGCy'C Cuts at: 1391 3258 3305 1391 Size: 1867 47 4072 Fragments arranged by size: 4072 1867 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2058 2578 2835 3171 928 Size: 971 159 520 257 336 3743 Fragments arranged by size: 3743 971 520 336 257 159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2112 2146 4177 4211 1903 Size: 34 175 34 2031 34 3678 Fragments arranged by size: 3678 2031 175 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3878 5088 3878 Size: 1210 4776 BclI T'GATC_A Cuts at: 1548 1548 Size: 5986 BfrBI ATG'CAT Cuts at: 2348 2348 Size: 5986 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2075 2552 3268 Size: 107 122 71 631 605 403 477 716 Cuts at: 3268 4597 136 Size: 1329 1525 Fragments arranged by size: 1525 1329 716 631 605 477 403 122 107 71 BglII A'GATC_T Cuts at: 5981 5981 Size: 5986 BlpI GC'TnA_GC Cuts at: 3403 3403 Size: 5986 Bme1580I G_kGCm'C Cuts at: 1790 4047 5203 1790 Size: 2257 1156 2573 Fragments arranged by size: 2573 2257 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5986 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3717 4675 442 Size: 416 53 83 399 24 2300 958 1753 Fragments arranged by size: 2300 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5986 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2711 2711 Size: 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4646 1239 Size: 3407 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4111 4976 5274 4111 Size: 865 298 4823 Fragments arranged by size: 4823 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2411 4649 882 Size: 617 912 2238 2219 Fragments arranged by size: 2238 2219 912 617 BsaAI yAC'GTr Cuts at: 493 1204 2229 3777 493 Size: 711 1025 1548 2702 Fragments arranged by size: 2702 1548 1025 711 BsaBI GATnn'nnATC Cuts at: 5980 5980 Size: 5986 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3793 4175 4810 275 Size: 53 83 186 936 2260 382 635 1451 Fragments arranged by size: 2260 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4419 4817 5091 5238 5649 4419 Size: 398 274 147 411 4756 Fragments arranged by size: 4756 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5761 Fragments arranged by size: 5761 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2687 1186 Size: 1501 4485 BseYI C'CCAG_C Cuts at: 5189 5685 5189 Size: 496 5490 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2152 1163 Size: 573 416 4997 Fragments arranged by size: 4997 573 416 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2782 3436 4197 5225 1540 Size: 275 186 781 654 761 1028 2301 Fragments arranged by size: 2301 1028 781 761 654 275 186 BsiHKAI G_wGCw'C Cuts at: 2692 2994 4047 4132 5203 2692 Size: 302 1053 85 1071 3475 Fragments arranged by size: 3475 1071 1053 302 85 BsmI GAATG_Cn' Cuts at: 2468 3497 3590 2468 Size: 1029 93 4864 Fragments arranged by size: 4864 1029 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2044 2610 2044 Size: 566 5420 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2692 2994 3258 3305 4047 4132 5203 Size: 399 902 302 264 47 742 85 1071 Cuts at: 5203 1391 Size: 2174 Fragments arranged by size: 2174 1071 902 742 399 302 264 85 47 BspEI T'CCGG_A Cuts at: 5649 5649 Size: 5986 BspHI T'CATG_A Cuts at: 3875 5605 3875 Size: 1730 4256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3341 844 Size: 2497 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3873 1394 Size: 429 2050 3507 Fragments arranged by size: 3507 2050 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4481 4655 65 Size: 1183 3233 174 1396 Fragments arranged by size: 3233 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4630 4817 1700 Size: 110 2820 187 2869 Fragments arranged by size: 2869 2820 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5986 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5986 BssSI C'ACGA_G Cuts at: 4046 5058 4046 Size: 1012 4974 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5986 BstBI TT'CG_AA Cuts at: 4790 4790 Size: 5986 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5986 BstXI CCAn_nnnn'nTGG Cuts at: 2233 2233 Size: 5986 BstYI r'GATC_y Cuts at: 1972 3316 3683 4068 4085 5537 5623 5635 5981 Size: 1344 367 385 17 1452 86 12 346 Cuts at: 5981 1972 Size: 1977 Fragments arranged by size: 1977 1452 1344 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2073 2332 513 Size: 552 939 69 259 4167 Fragments arranged by size: 4167 939 552 259 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5247 Fragments arranged by size: 5247 683 56 BtsI GCAGTG_nn' Cuts at: 2017 2916 3502 4296 4316 2017 Size: 899 586 794 20 3687 Fragments arranged by size: 3687 899 794 586 20 ClaI AT'CG_AT Cuts at: 1554 2517 2778 1554 Size: 963 261 4762 Fragments arranged by size: 4762 963 261 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5951 DraI TTT'AAA Cuts at: 3290 3290 Size: 5986 DraIII CAC_nnn'GTG Cuts at: 2229 2671 2229 Size: 442 5544 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4993 809 Size: 507 3677 1802 Fragments arranged by size: 3677 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2158 2544 Size: 54 1425 50 275 18 237 91 386 Cuts at: 2544 3433 4322 5951 8 Size: 889 889 1629 43 Fragments arranged by size: 1629 1425 889 889 386 275 237 91 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3433 1537 Size: 275 1621 4090 Fragments arranged by size: 4090 1621 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2047 2099 2411 2561 2994 3916 1353 Size: 694 52 312 150 433 922 3423 Fragments arranged by size: 3423 922 694 433 312 150 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4573 4947 5093 387 Size: 1064 138 2984 374 146 1280 Fragments arranged by size: 2984 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2028 2572 2739 4049 4646 5433 5701 Size: 393 396 544 167 1310 597 787 268 Cuts at: 5701 1239 Size: 1524 Fragments arranged by size: 1524 1310 787 597 544 396 393 268 167 EcoO109I rG'GnC_Cy Cuts at: 2338 3261 2338 Size: 923 5063 EcoRI G'AATT_C Cuts at: 3295 3673 3295 Size: 378 5608 FspI TGC'GCA Cuts at: 4492 4492 Size: 5986 HaeII r_GCGC'y Cuts at: 1678 5133 1678 Size: 3455 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4627 4659 4701 4733 1373 Size: 32 135 32 3055 32 42 32 2626 Fragments arranged by size: 3055 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2383 3330 3576 669 Size: 988 281 445 947 246 3079 Fragments arranged by size: 3079 988 947 445 281 246 HindIII A'AGCT_T Cuts at: 748 2364 748 Size: 1616 4370 HpaI GTT'AAC Cuts at: 3576 3576 Size: 5986 KpnI G_GTAC'C Cuts at: 2209 3311 2209 Size: 1102 4884 MfeI C'AATT_G Cuts at: 2469 3585 2469 Size: 1116 4870 MluI A'CGCG_T Cuts at: 5862 5862 Size: 5986 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3258 5100 5284 1305 Size: 1953 1842 184 2007 Fragments arranged by size: 2007 1953 1842 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2069 2160 2546 10 Size: 54 1425 343 237 91 386 3450 Fragments arranged by size: 3450 1425 386 343 237 91 54 MslI CAynn'nnrTG Cuts at: 518 1166 2166 2183 2231 2486 2921 2979 3944 Size: 648 1000 17 48 255 435 58 965 Cuts at: 3944 4303 4462 518 Size: 359 159 2042 Fragments arranged by size: 2042 1000 965 648 435 359 255 159 58 48 17 MspA1I CmG'CkG Cuts at: 1606 3402 4079 5227 5472 1606 Size: 1796 677 1148 245 2120 Fragments arranged by size: 2120 1796 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2073 2332 513 Size: 552 939 69 259 4167 Fragments arranged by size: 4167 939 552 259 69 NdeI CA'TA_TG Cuts at: 387 387 Size: 5986 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5986 NotI GC'GGCC_GC Cuts at: 3433 3433 Size: 5986 NsiI A_TGCA'T Cuts at: 2350 2350 Size: 5986 NspI r_CATG'y Cuts at: 1132 1229 3344 4889 1132 Size: 97 2115 1545 2229 Fragments arranged by size: 2229 2115 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5986 PciI A'CATG_T Cuts at: 4885 4885 Size: 5986 PflMI CCAn_nnn'nTGG Cuts at: 1108 2671 1108 Size: 1563 4423 PmeI GTTT'AAAC Cuts at: 3290 3290 Size: 5986 PmlI CAC'GTG Cuts at: 2229 2229 Size: 5986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4055 4087 5568 5600 4055 Size: 32 1481 32 4441 Fragments arranged by size: 4441 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2338 2338 Size: 5986 PshAI GACnn'nnGTC Cuts at: 1613 2582 1613 Size: 969 5017 PsiI TTA'TAA Cuts at: 3556 3556 Size: 5986 PstI C_TGCA'G Cuts at: 830 3338 830 Size: 2508 3478 PvuI CG_AT'CG Cuts at: 2001 2782 2001 Size: 781 5205 SalI G'TCGA_C Cuts at: 1936 3328 1936 Size: 1392 4594 SbfI CC_TGCA'GG Cuts at: 3338 3338 Size: 5986 ScaI AGT'ACT Cuts at: 4234 5946 4234 Size: 1712 4274 SfcI C'TryA_G Cuts at: 826 1045 2029 2808 3334 4469 5150 5341 826 Size: 219 984 779 526 1135 681 191 1471 Fragments arranged by size: 1471 1135 984 779 681 526 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5986 SmaI CCC'GGG Cuts at: 1872 3313 1872 Size: 1441 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4090 4991 5253 820 Size: 197 926 2147 901 262 1553 Fragments arranged by size: 2147 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3777 493 Size: 3284 2702 SpeI A'CTAG_T Cuts at: 152 3197 152 Size: 3045 2941 SphI G_CATG'C Cuts at: 1132 3344 1132 Size: 2212 3774 SspI AAT'ATT Cuts at: 5 52 3910 5 Size: 47 3858 2081 Fragments arranged by size: 3858 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5986 StyI C'CwwG_G Cuts at: 513 1065 2004 2073 2332 3425 513 Size: 552 939 69 259 1093 3074 Fragments arranged by size: 3074 1093 939 552 259 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2177 4013 4030 4183 4368 4813 325 Size: 1255 597 1836 17 153 185 445 1498 Fragments arranged by size: 1836 1498 1255 597 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4232 5944 96 Size: 274 80 33 51 1443 2255 1712 138 Fragments arranged by size: 2255 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5946 5978 5946 Size: 32 5954 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5986 XbaI T'CTAG_A Cuts at: 3250 3322 3250 Size: 72 5914 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5986 XmaI C'CCGG_G Cuts at: 1870 3311 1870 Size: 1441 4545 XmnI GAAnn'nnTTC Cuts at: 2713 4115 2713 Size: 1402 4584 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3794 276 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI