Restriction map for
pFN21AE0240
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglII BlnI BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KspI MaeII MamI
MfeI MluI MroI MseI MslI
MunI MvnI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 31 enzymes) #
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Acc65I 40 GGTACC G^GTACC
AcsI 106 GAATTT [GA]^AATT[CT]
AocI 1210 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1028 AGCGCT AGC^GCT
ApoI 106 GAATTT [GA]^AATT[CT]
Asp700I 864 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 40 GGTACC G^GTACC
BalI 731 TGGCCA TGG^CCA
BbsI 877 GAAGAC GAAGAC(2/6)
BciVI 1227 GGATAC GTATCC(6/5)
BglI 448 GCCACCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 877 GAAGAC GAAGAC(2/6)
BsrFI 961 GCCGGC [GA]^CCGG[CT]
BstAPI 1142 GCAGTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1210 CCTCAGG CC^T[ACGT]AGG
Cfr10I 961 GCCGGC [GA]^CCGG[CT]
EaeI 731 TGGCCA [CT]^GGCC[GA]
EarI 1324 CTCTTC CTCTTC(1/4)
Eco47III 1028 AGCGCT AGC^GCT
Eco81I 1210 CCTCAGG CC^T[ACGT]AGG
HaeII 1028 AGCGCT [GA]GCGC^[CT]
KpnI 40 GGTACC GGTAC^C
Ksp632I 1324 CTCTTC CTCTTC(1/4)
MluNI 731 TGGCCA TGG^CCA
MscI 731 TGGCCA TGG^CCA
NaeI 961 GCCGGC GCC^GGC
NgoMIV 961 GCCGGC G^CCGGC
TaqI 1013 TCGA T^CGA
TfiI 1059 GAATC G^A[AT]TC
TthHB8I 1013 TCGA T^CGA
XmnI 864 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwNI 434 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1124 CAGCCCCTG
AspHI 188 GTGCTC G[AT]GC[AT]^C
1298 GTGCTC
AvaI 328 CCCGGG C^[CT]CG[GA]G
607 CCCGGG
BanI 40 GGTACC G^G[CT][GA]CC
661 GGTGCC
BfaI 517 CTAG C^TAG
859 CTAG
BlpI 353 GCTCAGC GC^T[ACGT]AGC
1031 GCTCAGC
Bpu1102I 353 GCTCAGC GC^T[ACGT]AGC
1031 GCTCAGC
BsaI 196 GAGACC GGTCTC(1/5)
773 GAGACC
BsaWI 128 ACCGGA [AT]^CCGG[AT]
173 TCCGGT
BsgI 402 CTGCAC GTGCAG(16/14)
177 GTGCAG
BsiHKAI 188 GTGCTC G[AT]GC[AT]^C
1298 GTGCTC
BsoBI 328 CCCGGG C^[CT]CG[GA]G
607 CCCGGG
BspMI 802 ACCTGC ACCTGC(4/8)
1173 ACCTGC
BstEII 930 GGTGACC G^GT[ACGT]ACC
1311 GGTGACC
BstPI 930 GGTGACC G^GT[ACGT]ACC
1311 GGTGACC
BstXI 294 CCAGAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
632 CCATGGGTCTGG
BstYI 131 GGATCT [GA]^GATC[CT]
1166 AGATCC
CelII 353 GCTCAGC GC^T[ACGT]AGC
1031 GCTCAGC
DsaI 632 CCATGG C^C[GA][CT]GG
1184 CCATGG
EcoO65I 930 GGTGACC G^GT[ACGT]ACC
1311 GGTGACC
MaeI 517 CTAG C^TAG
859 CTAG
MflI 131 GGATCT [GA]^GATC[CT]
1166 AGATCC
NcoI 632 CCATGG C^CATGG
1184 CCATGG
PflMI 918 CCAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1266 CCAGACTTTGG
PvuII 847 CAGCTG CAG^CTG
1160 CAGCTG
SmaI 328 CCCGGG CCC^GGG
607 CCCGGG
Van91I 918 CCAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1266 CCAGACTTTGG
XhoII 131 GGATCT [GA]^GATC[CT]
1166 AGATCC
XmaCI 328 CCCGGG C^CCGGG
607 CCCGGG
XmaI 328 CCCGGG C^CCGGG
607 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I Tsp509I TspRI