(Circular) (Six-base) MAPSORT of: pFN21AE0240.seq Check: 5827 from: 1 to: 6175 pFN21AE0240 6175 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3985 278 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3518 1937 Size: 1581 4594 Acc65I G'GTAC_C Cuts at: 2045 3496 2045 Size: 1451 4724 AclI AA'CG_TT Cuts at: 1563 4302 4675 1563 Size: 2739 373 3063 Fragments arranged by size: 3063 2739 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3316 4238 5622 5890 1632 Size: 1684 922 1384 268 1917 Fragments arranged by size: 1917 1684 1384 922 268 AfeI AGC'GCT Cuts at: 3035 3035 Size: 6175 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5978 AflIII A'CryG_T Cuts at: 1203 5074 6051 1203 Size: 3871 977 1327 Fragments arranged by size: 3871 1327 977 AgeI A'CCGG_T Cuts at: 5006 5006 Size: 6175 AhdI GACnn_n'nnGTC Cuts at: 4904 4904 Size: 6175 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6175 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6175 AlwNI CAG_nnn'CTG Cuts at: 2444 3134 3918 5490 2444 Size: 690 784 1572 3129 Fragments arranged by size: 3129 1572 784 690 ApaI G_GGCC'C Cuts at: 2727 2796 3003 2727 Size: 69 207 5899 Fragments arranged by size: 5899 207 69 ApaLI G'TGCA_C Cuts at: 4232 5388 4232 Size: 1156 5019 ApoI r'AATT_y Cuts at: 1493 2111 3484 3710 3862 5971 1493 Size: 618 1373 226 152 2109 1697 Fragments arranged by size: 2109 1697 1373 618 226 152 AseI AT'TA_AT Cuts at: 160 1032 4729 160 Size: 872 3697 1606 Fragments arranged by size: 3697 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 AvaI C'yCGr_G Cuts at: 1870 1943 2333 2612 3500 1870 Size: 73 390 279 888 4545 Fragments arranged by size: 4545 888 390 279 73 BamHI G'GATC_C Cuts at: 3505 3872 3505 Size: 367 5808 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 2666 3496 4951 618 Size: 325 217 627 258 621 830 1455 1842 Fragments arranged by size: 1842 1455 830 627 621 325 258 217 BanII G_rGCy'C Cuts at: 1391 2360 2503 2727 2796 3003 3249 3494 1391 Size: 969 143 224 69 207 246 245 4072 Fragments arranged by size: 4072 969 246 245 224 207 143 69 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2889 928 Size: 971 990 4214 Fragments arranged by size: 4214 990 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4366 4400 1903 Size: 34 2429 34 3678 Fragments arranged by size: 3678 2429 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3226 4067 5277 3226 Size: 841 1210 4124 Fragments arranged by size: 4124 1210 841 BclI T'GATC_A Cuts at: 1548 1548 Size: 6175 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2459 4786 136 Size: 107 122 71 631 605 787 2327 1525 Fragments arranged by size: 2327 1525 787 631 605 122 107 71 BglII A'GATC_T Cuts at: 6170 6170 Size: 6175 BlpI GC'TnA_GC Cuts at: 2359 3037 3592 2359 Size: 678 555 4942 Fragments arranged by size: 4942 678 555 Bme1580I G_kGCm'C Cuts at: 1790 2483 2671 2706 2727 2796 3003 3189 4236 Size: 693 188 35 21 69 207 186 1047 Cuts at: 4236 5392 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1047 693 207 188 186 69 35 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6175 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2561 2693 3288 Size: 416 53 83 399 24 1144 132 595 Cuts at: 3288 3906 4864 442 Size: 618 958 1753 Fragments arranged by size: 1753 1144 958 618 595 416 399 132 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6175 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2219 2328 2466 2479 2515 2589 2876 Size: 821 159 109 138 13 36 74 287 Cuts at: 2876 4835 1239 Size: 1959 2579 Fragments arranged by size: 2579 1959 821 287 159 138 109 74 36 13 Bpu10I CC'TnA_GC Cuts at: 2373 2373 Size: 6175 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4300 5165 5463 4300 Size: 865 298 5012 Fragments arranged by size: 5012 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2195 2772 4838 882 Size: 617 696 577 2066 2219 Fragments arranged by size: 2219 2066 696 617 577 BsaAI yAC'GTr Cuts at: 493 1204 3966 493 Size: 711 2762 2702 Fragments arranged by size: 2762 2702 711 BsaBI GATnn'nnATC Cuts at: 6169 6169 Size: 6175 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3982 4364 4999 275 Size: 53 83 186 936 2449 382 635 1451 Fragments arranged by size: 2449 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2133 2178 4608 5006 5280 5427 5838 2133 Size: 45 2430 398 274 147 411 2470 Fragments arranged by size: 2470 2430 411 398 274 147 45 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2644 2674 1178 Size: 30 165 30 1241 30 4679 Fragments arranged by size: 4679 1241 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2354 2735 2873 1186 Size: 1168 381 138 4488 Fragments arranged by size: 4488 1168 381 138 BseYI C'CCAG_C Cuts at: 2468 2943 5378 5874 2468 Size: 475 2435 496 2769 Fragments arranged by size: 2769 2435 496 475 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2203 2392 1163 Size: 573 467 189 4946 Fragments arranged by size: 4946 573 467 189 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3625 4386 5414 1540 Size: 275 186 1624 761 1028 2301 Fragments arranged by size: 2301 1624 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2197 3307 4236 4321 5392 2197 Size: 1110 929 85 1071 2980 Fragments arranged by size: 2980 1110 1071 929 85 BsmI GAATG_Cn' Cuts at: 3686 3779 3686 Size: 93 6082 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2197 2360 2483 2503 2671 2706 2727 Size: 399 407 163 123 20 168 35 21 Cuts at: 2727 2796 3003 3189 3249 3307 3494 4236 4321 Size: 69 207 186 60 58 187 742 85 Cuts at: 4321 5392 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 407 399 207 187 186 168 163 123 85 69 60 58 35 21 20 BspEI T'CCGG_A Cuts at: 5838 5838 Size: 6175 BspHI T'CATG_A Cuts at: 4064 5794 4064 Size: 1730 4445 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2816 3187 3530 844 Size: 1972 371 343 3489 Fragments arranged by size: 3489 1972 371 343 BsrBI CCG'CTC Cuts at: 1394 1823 2493 2529 2844 4062 1394 Size: 429 670 36 315 1218 3507 Fragments arranged by size: 3507 1218 670 429 315 36 BsrDI GCAATG_nn' Cuts at: 65 1248 4670 4844 65 Size: 1183 3422 174 1396 Fragments arranged by size: 3422 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2966 4819 5006 1700 Size: 110 1156 1853 187 2869 Fragments arranged by size: 2869 1853 1156 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6175 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6175 BssSI C'ACGA_G Cuts at: 4235 5247 4235 Size: 1012 5163 BstAPI GCAn_nnn'nTGC Cuts at: 794 3153 794 Size: 2359 3816 BstBI TT'CG_AA Cuts at: 4979 4979 Size: 6175 BstEII G'GTnAC_C Cuts at: 1184 2935 3316 1184 Size: 1751 381 4043 Fragments arranged by size: 4043 1751 381 BstXI CCAn_nnnn'nTGG Cuts at: 2306 2644 2306 Size: 338 5837 BstYI r'GATC_y Cuts at: 1972 2136 3171 3505 3872 4257 4274 5726 5812 Size: 164 1035 334 367 385 17 1452 86 Cuts at: 5812 5824 6170 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1035 385 367 346 334 164 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3216 3216 Size: 6175 BtgI C'CryG_G Cuts at: 513 1065 2004 2637 3189 513 Size: 552 939 633 552 3499 Fragments arranged by size: 3499 939 633 552 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5436 Fragments arranged by size: 5436 683 56 BtsI GCAGTG_nn' Cuts at: 3404 3433 3691 4485 4505 3404 Size: 29 258 794 20 5074 Fragments arranged by size: 5074 794 258 29 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6175 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6140 DraI TTT'AAA Cuts at: 3479 3479 Size: 6175 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5182 809 Size: 507 3866 1802 Fragments arranged by size: 3866 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2736 3622 4511 Size: 54 1425 50 275 18 906 886 889 Cuts at: 4511 6140 8 Size: 1629 43 Fragments arranged by size: 1629 1425 906 889 886 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3622 1537 Size: 275 1810 4090 Fragments arranged by size: 4090 1810 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3335 4105 1353 Size: 1982 770 3423 Fragments arranged by size: 3423 1982 770 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3009 4762 5136 5282 387 Size: 1064 138 1420 1753 374 146 1280 Fragments arranged by size: 1753 1420 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2219 2328 2466 2479 2515 2589 Size: 393 428 159 109 138 13 36 74 Cuts at: 2589 2876 3316 4238 4835 5622 5890 1239 Size: 287 440 922 597 787 268 1524 Fragments arranged by size: 1524 922 787 597 440 428 393 287 268 159 138 109 74 36 13 EcoO109I rG'GnC_Cy Cuts at: 2330 2535 2595 2723 2724 2792 2793 2999 3459 Size: 205 60 128 1 68 1 206 460 Cuts at: 3459 2330 Size: 5046 Fragments arranged by size: 5046 460 206 205 128 68 60 1 1 EcoRI G'AATT_C Cuts at: 3484 3862 3484 Size: 378 5797 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2089 2089 Size: 6175 FspI TGC'GCA Cuts at: 4681 4681 Size: 6175 HaeII r_GCGC'y Cuts at: 1678 3037 5322 1678 Size: 1359 2285 2531 Fragments arranged by size: 2531 2285 1359 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4816 4848 4890 4922 1373 Size: 32 135 32 3244 32 42 32 2626 Fragments arranged by size: 3244 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3519 3765 669 Size: 988 281 1581 246 3079 Fragments arranged by size: 3079 1581 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6175 HpaI GTT'AAC Cuts at: 3765 3765 Size: 6175 KpnI G_GTAC'C Cuts at: 2049 3500 2049 Size: 1451 4724 MfeI C'AATT_G Cuts at: 3774 3774 Size: 6175 MluI A'CGCG_T Cuts at: 6051 6051 Size: 6175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5289 5473 1305 Size: 3984 184 2007 Fragments arranged by size: 3984 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2738 10 Size: 54 1425 343 906 3447 Fragments arranged by size: 3447 1425 906 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4133 4492 4651 518 Size: 648 2967 359 159 2042 Fragments arranged by size: 2967 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2854 3041 3167 3591 4268 5416 5661 1606 Size: 1248 187 126 424 677 1148 245 2120 Fragments arranged by size: 2120 1248 1148 677 424 245 187 126 NaeI GCC'GGC Cuts at: 2968 2968 Size: 6175 NcoI C'CATG_G Cuts at: 513 1065 2004 2637 3189 513 Size: 552 939 633 552 3499 Fragments arranged by size: 3499 939 633 552 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6175 NgoMIV G'CCGG_C Cuts at: 2966 2966 Size: 6175 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6175 NotI GC'GGCC_GC Cuts at: 3622 3622 Size: 6175 NspI r_CATG'y Cuts at: 1132 1229 3533 5078 1132 Size: 97 2304 1545 2229 Fragments arranged by size: 2304 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6175 PciI A'CATG_T Cuts at: 5074 5074 Size: 6175 PflMI CCAn_nnn'nTGG Cuts at: 1108 2929 3277 1108 Size: 1821 348 4006 Fragments arranged by size: 4006 1821 348 PmeI GTTT'AAAC Cuts at: 3479 3479 Size: 6175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2235 2267 4244 4276 5757 5789 2235 Size: 32 1977 32 1481 32 2621 Fragments arranged by size: 2621 1977 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2330 2595 3459 2330 Size: 265 864 5046 Fragments arranged by size: 5046 864 265 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6175 PsiI TTA'TAA Cuts at: 3745 3745 Size: 6175 PspOMI G'GGCC_C Cuts at: 2723 2792 2999 2723 Size: 69 207 5899 Fragments arranged by size: 5899 207 69 PstI C_TGCA'G Cuts at: 830 2329 3130 3166 3527 830 Size: 1499 801 36 361 3478 Fragments arranged by size: 3478 1499 801 361 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6175 PvuII CAG'CTG Cuts at: 2854 3167 2854 Size: 313 5862 SalI G'TCGA_C Cuts at: 1936 3517 1936 Size: 1581 4594 SbfI CC_TGCA'GG Cuts at: 3527 3527 Size: 6175 ScaI AGT'ACT Cuts at: 4423 6135 4423 Size: 1712 4463 SfcI C'TryA_G Cuts at: 826 1045 2325 3126 3162 3523 4658 5339 5530 Size: 219 1280 801 36 361 1135 681 191 Cuts at: 5530 826 Size: 1471 Fragments arranged by size: 1471 1280 1135 801 681 361 219 191 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6175 SmaI CCC'GGG Cuts at: 1872 2335 2614 3502 1872 Size: 463 279 888 4545 Fragments arranged by size: 4545 888 463 279 SmlI C'TyrA_G Cuts at: 820 1017 1943 4279 5180 5442 820 Size: 197 926 2336 901 262 1553 Fragments arranged by size: 2336 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3966 493 Size: 3473 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6175 SphI G_CATG'C Cuts at: 1132 3533 1132 Size: 2401 3774 SspI AAT'ATT Cuts at: 5 52 4099 5 Size: 47 4047 2081 Fragments arranged by size: 4047 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6175 StyI C'CwwG_G Cuts at: 513 1065 2004 2637 3189 3373 3614 513 Size: 552 939 633 552 184 241 3074 Fragments arranged by size: 3074 939 633 552 552 241 184 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3040 3302 4202 4219 4372 4557 5002 Size: 1255 1460 262 900 17 153 185 445 Cuts at: 5002 325 Size: 1498 Fragments arranged by size: 1498 1460 1255 900 445 262 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2750 4421 6133 Size: 274 80 33 51 1443 773 1671 1712 Cuts at: 6133 96 Size: 138 Fragments arranged by size: 1712 1671 1443 773 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6135 6167 6135 Size: 32 6143 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6175 XbaI T'CTAG_A Cuts at: 3511 3511 Size: 6175 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6175 XmaI C'CCGG_G Cuts at: 1870 2333 2612 3500 1870 Size: 463 279 888 4545 Fragments arranged by size: 4545 888 463 279 XmnI GAAnn'nnTTC Cuts at: 2873 4304 2873 Size: 1431 4744 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3983 276 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI