Restriction map for
pFN21AE0240
(Linear) (Six-base) MAPSORT of: KIEE0240.seq Check: 5488 from: 1 to: 1470
KIEE0240.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
Acc65I G'GTAC_C
Cuts at: 0 40 1470
Size: 40 1430
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1311 1470
Size: 1311 159
AfeI AGC'GCT
Cuts at: 0 1030 1470
Size: 1030 440
AlwNI CAG_nnn'CTG
Cuts at: 0 439 1129 1470
Size: 439 690 341
Fragments arranged by size:
690 439 341
ApaI G_GGCC'C
Cuts at: 0 722 791 998 1470
Size: 722 69 207 472
Fragments arranged by size:
722 472 207 69
ApoI r'AATT_y
Cuts at: 0 106 1470
Size: 106 1364
AvaI C'yCGr_G
Cuts at: 0 328 607 1470
Size: 328 279 863
Fragments arranged by size:
863 328 279
BanI G'GyrC_C
Cuts at: 0 40 661 1470
Size: 40 621 809
Fragments arranged by size:
809 621 40
BanII G_rGCy'C
Cuts at: 0 355 498 722 791 998 1244 1470
Size: 355 143 224 69 207 246 226
Fragments arranged by size:
355 246 226 224 207 143 69
BbsI GAAGACnn'nnnn_
Cuts at: 0 884 1470
Size: 884 586
BciVI GTATCCnnnnn_n'
Cuts at: 0 1221 1470
Size: 1221 249
BglI GCCn_nnn'nGGC
Cuts at: 0 454 1470
Size: 454 1016
BlpI GC'TnA_GC
Cuts at: 0 354 1032 1470
Size: 354 678 438
Fragments arranged by size:
678 438 354
Bme1580I G_kGCm'C
Cuts at: 0 478 666 701 722 791 998 1184 1470
Size: 478 188 35 21 69 207 186 286
Fragments arranged by size:
478 286 207 188 186 69 35 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 556 688 1283 1470
Size: 556 132 595 187
Fragments arranged by size:
595 556 187 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 214 323 461 474 510 584 871
Size: 55 159 109 138 13 36 74 287
Cuts at: 871 1470
Size: 599
Fragments arranged by size:
599 287 159 138 109 74 55 36
13
Bpu10I CC'TnA_GC
Cuts at: 0 368 1470
Size: 368 1102
BsaI GGTCTCn'nnnn_
Cuts at: 0 190 767 1470
Size: 190 577 703
Fragments arranged by size:
703 577 190
BsaWI w'CCGG_w
Cuts at: 0 128 173 1470
Size: 128 45 1297
Fragments arranged by size:
1297 128 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 639 669 1470
Size: 639 30 801
Fragments arranged by size:
801 639 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 349 730 868 1470
Size: 349 381 138 602
Fragments arranged by size:
602 381 349 138
BseYI C'CCAG_C
Cuts at: 0 463 938 1470
Size: 463 475 532
Fragments arranged by size:
532 475 463
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 387 1470
Size: 198 189 1083
Fragments arranged by size:
1083 198 189
BsiHKAI G_wGCw'C
Cuts at: 0 192 1302 1470
Size: 192 1110 168
Fragments arranged by size:
1110 192 168
Bsp1286I G_dGCh'C
Cuts at: 0 192 355 478 498 666 701 722 791
Size: 192 163 123 20 168 35 21 69
Cuts at: 791 998 1184 1244 1302 1470
Size: 207 186 60 58 168
Fragments arranged by size:
207 192 186 168 168 163 123 69
60 58 35 21 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 811 1182 1470
Size: 811 371 288
Fragments arranged by size:
811 371 288
BsrBI CCG'CTC
Cuts at: 0 488 524 839 1470
Size: 488 36 315 631
Fragments arranged by size:
631 488 315 36
BsrFI r'CCGG_y
Cuts at: 0 961 1470
Size: 961 509
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1148 1470
Size: 1148 322
BstEII G'GTnAC_C
Cuts at: 0 930 1311 1470
Size: 930 381 159
Fragments arranged by size:
930 381 159
BstXI CCAn_nnnn'nTGG
Cuts at: 0 301 639 1470
Size: 301 338 831
Fragments arranged by size:
831 338 301
BstYI r'GATC_y
Cuts at: 0 131 1166 1470
Size: 131 1035 304
Fragments arranged by size:
1035 304 131
Bsu36I CC'TnA_GG
Cuts at: 0 1211 1470
Size: 1211 259
BtgI C'CryG_G
Cuts at: 0 632 1184 1470
Size: 632 552 286
Fragments arranged by size:
632 552 286
BtsI GCAGTG_nn'
Cuts at: 0 1399 1428 1470
Size: 1399 29 42
Fragments arranged by size:
1399 42 29
EaeI y'GGCC_r
Cuts at: 0 731 1470
Size: 731 739
EarI CTCTTCn'nnn_
Cuts at: 0 1330 1470
Size: 1330 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1004 1470
Size: 1004 466
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 214 323 461 474 510 584 871
Size: 55 159 109 138 13 36 74 287
Cuts at: 871 1311 1470
Size: 440 159
Fragments arranged by size:
440 287 159 159 138 109 74 55
36 13
EcoO109I rG'GnC_Cy
Cuts at: 0 325 530 590 718 719 787 788 994
Size: 325 205 60 128 1 68 1 206
Cuts at: 994 1454 1470
Size: 460 16
Fragments arranged by size:
460 325 206 205 128 68 60 16
1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 84 1470
Size: 84 1386
HaeII r_GCGC'y
Cuts at: 0 1032 1470
Size: 1032 438
KpnI G_GTAC'C
Cuts at: 0 44 1470
Size: 44 1426
MscI TGG'CCA
Cuts at: 0 733 1470
Size: 733 737
MspA1I CmG'CkG
Cuts at: 0 849 1036 1162 1470
Size: 849 187 126 308
Fragments arranged by size:
849 308 187 126
NaeI GCC'GGC
Cuts at: 0 963 1470
Size: 963 507
NcoI C'CATG_G
Cuts at: 0 632 1184 1470
Size: 632 552 286
Fragments arranged by size:
632 552 286
NgoMIV G'CCGG_C
Cuts at: 0 961 1470
Size: 961 509
PflMI CCAn_nnn'nTGG
Cuts at: 0 924 1272 1470
Size: 924 348 198
Fragments arranged by size:
924 348 198
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 230 262 1470
Size: 230 32 1208
Fragments arranged by size:
1208 230 32
PpuMI rG'GwC_Cy
Cuts at: 0 325 590 1454 1470
Size: 325 265 864 16
Fragments arranged by size:
864 325 265 16
PspOMI G'GGCC_C
Cuts at: 0 718 787 994 1470
Size: 718 69 207 476
Fragments arranged by size:
718 476 207 69
PstI C_TGCA'G
Cuts at: 0 324 1125 1161 1470
Size: 324 801 36 309
Fragments arranged by size:
801 324 309 36
PvuII CAG'CTG
Cuts at: 0 849 1162 1470
Size: 849 313 308
Fragments arranged by size:
849 313 308
SfcI C'TryA_G
Cuts at: 0 320 1121 1157 1470
Size: 320 801 36 313
Fragments arranged by size:
801 320 313 36
SmaI CCC'GGG
Cuts at: 0 330 609 1470
Size: 330 279 861
Fragments arranged by size:
861 330 279
StyI C'CwwG_G
Cuts at: 0 632 1184 1368 1470
Size: 632 552 184 102
Fragments arranged by size:
632 552 184 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1035 1297 1470
Size: 1035 262 173
Fragments arranged by size:
1035 262 173
TatI w'GTAC_w
Cuts at: 0 745 1470
Size: 745 725
XmaI C'CCGG_G
Cuts at: 0 328 607 1470
Size: 328 279 863
Fragments arranged by size:
863 328 279
XmnI GAAnn'nnTTC
Cuts at: 0 868 1470
Size: 868 602
Enzymes that do cut:
Acc65I AcuI AfeI AlwNI ApaI ApoI AvaI BanI
BanII BbsI BciVI BglI BlpI Bme1580I BmrI BpmI
Bpu10I BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI EaeI EarI EciI Eco57MI EcoO109I
FalI HaeII KpnI MscI MspA1I NaeI NcoI NgoMIV
PflMI PpiI PpuMI PspOMI PstI PvuII SfcI SmaI
StyI TaqII TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BclI BfrBI BglII
BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI MfeI MluI
MmeI MslI NarI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XcmI XhoI ZraI