(Circular) (Six-base) MAPSORT of: pFN21AE0236.seq Check: 2446 from: 1 to: 5533 pFN21AE0236 5533 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3343 278 Size: 53 83 186 936 1807 2468 Fragments arranged by size: 2468 1807 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2876 1937 Size: 939 4594 Acc65I G'GTAC_C Cuts at: 2854 2854 Size: 5533 AclI AA'CG_TT Cuts at: 1563 2396 3660 4033 1563 Size: 833 1264 373 3063 Fragments arranged by size: 3063 1264 833 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2497 3596 4980 5248 1632 Size: 865 1099 1384 268 1917 Fragments arranged by size: 1917 1384 1099 865 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5336 AflIII A'CryG_T Cuts at: 1203 4432 5409 1203 Size: 3229 977 1327 Fragments arranged by size: 3229 1327 977 AgeI A'CCGG_T Cuts at: 4364 4364 Size: 5533 AhdI GACnn_n'nnGTC Cuts at: 4262 4262 Size: 5533 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5533 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5533 AlwNI CAG_nnn'CTG Cuts at: 3276 4848 3276 Size: 1572 3961 ApaI G_GGCC'C Cuts at: 2570 2570 Size: 5533 ApaLI G'TGCA_C Cuts at: 3590 4746 3590 Size: 1156 4377 ApoI r'AATT_y Cuts at: 1493 2008 2842 3068 3220 5329 1493 Size: 515 834 226 152 2109 1697 Fragments arranged by size: 2109 1697 834 515 226 152 AseI AT'TA_AT Cuts at: 160 1032 4087 160 Size: 872 3055 1606 Fragments arranged by size: 3055 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 AvaI C'yCGr_G Cuts at: 1870 1943 2858 1870 Size: 73 915 4545 Fragments arranged by size: 4545 915 73 BamHI G'GATC_C Cuts at: 2863 3230 2863 Size: 367 5166 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2417 2854 4309 618 Size: 325 217 627 630 437 1455 1842 Fragments arranged by size: 1842 1455 630 627 437 325 217 BanII G_rGCy'C Cuts at: 1391 2382 2570 2852 1391 Size: 991 188 282 4072 Fragments arranged by size: 4072 991 282 188 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2527 928 Size: 971 628 3934 Fragments arranged by size: 3934 971 628 BbvCI CC'TCA_GC Cuts at: 2644 2644 Size: 5533 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3724 3758 1903 Size: 34 1787 34 3678 Fragments arranged by size: 3678 1787 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3425 4635 3425 Size: 1210 4323 BclI T'GATC_A Cuts at: 1548 1548 Size: 5533 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4144 136 Size: 107 122 71 631 605 2472 1525 Fragments arranged by size: 2472 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5528 5528 Size: 5533 BlpI GC'TnA_GC Cuts at: 2950 2950 Size: 5533 Bme1580I G_kGCm'C Cuts at: 1790 2420 2570 3594 4750 1790 Size: 630 150 1024 1156 2573 Fragments arranged by size: 2573 1156 1024 630 150 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5533 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2376 3264 4222 Size: 416 53 83 399 24 959 888 958 Cuts at: 4222 442 Size: 1753 Fragments arranged by size: 1753 959 958 888 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5533 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4193 1239 Size: 2954 2579 Bpu10I CC'TnA_GC Cuts at: 2644 2644 Size: 5533 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3658 4523 4821 3658 Size: 865 298 4370 Fragments arranged by size: 4370 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2552 4196 882 Size: 617 1053 1644 2219 Fragments arranged by size: 2219 1644 1053 617 BsaAI yAC'GTr Cuts at: 493 1204 3324 493 Size: 711 2120 2702 Fragments arranged by size: 2702 2120 711 BsaBI GATnn'nnATC Cuts at: 5527 5527 Size: 5533 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2352 3340 3722 4357 Size: 53 83 186 936 819 988 382 635 Cuts at: 4357 275 Size: 1451 Fragments arranged by size: 1451 988 936 819 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3966 4364 4638 4785 5196 3966 Size: 398 274 147 411 4303 Fragments arranged by size: 4303 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5308 Fragments arranged by size: 5308 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 2647 1186 Size: 1099 362 4072 Fragments arranged by size: 4072 1099 362 BseYI C'CCAG_C Cuts at: 2580 4736 5232 2580 Size: 2156 496 2881 Fragments arranged by size: 2881 2156 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4960 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2983 3744 4772 1540 Size: 275 186 982 761 1028 2301 Fragments arranged by size: 2301 1028 982 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3594 3679 4750 3594 Size: 85 1071 4377 Fragments arranged by size: 4377 1071 85 BsmI GAATG_Cn' Cuts at: 2025 3044 3137 2025 Size: 1019 93 4421 Fragments arranged by size: 4421 1019 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2382 2420 2570 2852 3594 3679 4750 Size: 399 592 38 150 282 742 85 1071 Cuts at: 4750 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 592 399 282 150 85 38 BspEI T'CCGG_A Cuts at: 5196 5196 Size: 5533 BspHI T'CATG_A Cuts at: 2313 2758 3422 5152 2313 Size: 445 664 1730 2694 Fragments arranged by size: 2694 1730 664 445 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2250 2888 844 Size: 1406 638 3489 Fragments arranged by size: 3489 1406 638 BsrBI CCG'CTC Cuts at: 1394 1823 3420 1394 Size: 429 1597 3507 Fragments arranged by size: 3507 1597 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4028 4202 65 Size: 1183 2780 174 1396 Fragments arranged by size: 2780 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4177 4364 1700 Size: 110 2367 187 2869 Fragments arranged by size: 2869 2367 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5533 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5533 BssSI C'ACGA_G Cuts at: 3593 4605 3593 Size: 1012 4521 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5533 BstBI TT'CG_AA Cuts at: 2392 4337 2392 Size: 1945 3588 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5533 BstYI r'GATC_y Cuts at: 1972 2863 3230 3615 3632 5084 5170 5182 5528 Size: 891 367 385 17 1452 86 12 346 Cuts at: 5528 1972 Size: 1977 Fragments arranged by size: 1977 1452 891 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2279 2279 Size: 5533 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4981 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2461 487 Size: 683 56 1235 3559 Fragments arranged by size: 3559 1235 683 56 BtsI GCAGTG_nn' Cuts at: 3049 3843 3863 3049 Size: 794 20 4719 Fragments arranged by size: 4719 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5533 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5498 DraI TTT'AAA Cuts at: 2410 2837 2410 Size: 427 5106 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2195 4540 809 Size: 507 879 2345 1802 Fragments arranged by size: 2345 1802 879 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2241 2980 3869 Size: 54 1425 50 275 18 411 739 889 Cuts at: 3869 5498 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 739 411 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2980 1537 Size: 275 1168 4090 Fragments arranged by size: 4090 1168 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2394 2453 2516 3463 1353 Size: 1041 59 63 947 3423 Fragments arranged by size: 3423 1041 947 63 59 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2236 4120 4494 4640 387 Size: 1064 138 647 1884 374 146 1280 Fragments arranged by size: 1884 1280 1064 647 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2497 3596 4193 4980 5248 1239 Size: 393 865 1099 597 787 268 1524 Fragments arranged by size: 1524 1099 865 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2197 2197 Size: 5533 EcoRI G'AATT_C Cuts at: 2008 2842 3220 2008 Size: 834 378 4321 Fragments arranged by size: 4321 834 378 FspI TGC'GCA Cuts at: 4039 4039 Size: 5533 HaeII r_GCGC'y Cuts at: 1678 4680 1678 Size: 3002 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4174 4206 4248 4280 1373 Size: 32 135 32 2602 32 42 32 2626 Fragments arranged by size: 2626 2602 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2877 3123 669 Size: 988 281 939 246 3079 Fragments arranged by size: 3079 988 939 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5533 HpaI GTT'AAC Cuts at: 3123 3123 Size: 5533 KpnI G_GTAC'C Cuts at: 2858 2858 Size: 5533 MfeI C'AATT_G Cuts at: 3132 3132 Size: 5533 MluI A'CGCG_T Cuts at: 5409 5409 Size: 5533 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4647 4831 1305 Size: 3342 184 2007 Fragments arranged by size: 3342 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3711 Fragments arranged by size: 3711 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2345 3491 3850 4009 518 Size: 648 1179 1146 359 159 2042 Fragments arranged by size: 2042 1179 1146 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2949 3626 4774 5019 1606 Size: 1343 677 1148 245 2120 Fragments arranged by size: 2120 1343 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4981 NdeI CA'TA_TG Cuts at: 387 387 Size: 5533 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5533 NotI GC'GGCC_GC Cuts at: 2980 2980 Size: 5533 NspI r_CATG'y Cuts at: 1132 1229 2456 2891 4436 1132 Size: 97 1227 435 1545 2229 Fragments arranged by size: 2229 1545 1227 435 97 PasI CC'CwG_GG Cuts at: 1348 2563 1348 Size: 1215 4318 PciI A'CATG_T Cuts at: 4432 4432 Size: 5533 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5533 PfoI T'CCnGG_A Cuts at: 2199 2490 2199 Size: 291 5242 PmeI GTTT'AAAC Cuts at: 2837 2837 Size: 5533 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2085 2117 3602 3634 5115 5147 2085 Size: 32 1485 32 1481 32 2471 Fragments arranged by size: 2471 1485 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2197 2197 Size: 5533 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5533 PsiI TTA'TAA Cuts at: 2141 3103 2141 Size: 962 4571 PspOMI G'GGCC_C Cuts at: 2566 2566 Size: 5533 PstI C_TGCA'G Cuts at: 830 2885 830 Size: 2055 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5533 SalI G'TCGA_C Cuts at: 1936 2875 1936 Size: 939 4594 SapI GCTCTTCn'nnn_ Cuts at: 2453 2453 Size: 5533 SbfI CC_TGCA'GG Cuts at: 2885 2885 Size: 5533 ScaI AGT'ACT Cuts at: 3781 5493 3781 Size: 1712 3821 SexAI A'CCwGG_T Cuts at: 2529 2529 Size: 5533 SfcI C'TryA_G Cuts at: 826 1045 2881 4016 4697 4888 826 Size: 219 1836 1135 681 191 1471 Fragments arranged by size: 1836 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5533 SmaI CCC'GGG Cuts at: 1872 2860 1872 Size: 988 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3637 4538 4800 820 Size: 197 926 1694 901 262 1553 Fragments arranged by size: 1694 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3324 493 Size: 2831 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5533 SphI G_CATG'C Cuts at: 1132 2891 1132 Size: 1759 3774 SspI AAT'ATT Cuts at: 5 52 2719 2810 3457 5 Size: 47 2667 91 647 2081 Fragments arranged by size: 2667 2081 647 91 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5533 StyI C'CwwG_G Cuts at: 513 1065 2972 513 Size: 552 1907 3074 Fragments arranged by size: 3074 1907 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2456 3560 3577 3730 3915 4360 325 Size: 1255 876 1104 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1104 876 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3779 5491 96 Size: 274 80 33 51 1443 1802 1712 138 Fragments arranged by size: 1802 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5493 5525 5493 Size: 32 5501 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5533 XbaI T'CTAG_A Cuts at: 2869 2869 Size: 5533 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5533 XmaI C'CCGG_G Cuts at: 1870 2858 1870 Size: 988 4545 XmnI GAAnn'nnTTC Cuts at: 2094 2546 3662 2094 Size: 452 1116 3965 Fragments arranged by size: 3965 1116 452 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3341 276 Size: 53 83 186 936 1807 2468 Fragments arranged by size: 2468 1807 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI