################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsoBI Bsp1286I BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AclI 452 AACGTT AA^CGTT AluI 569 AGCT AG^CT AlwI 388 GATCC GGATC(4/5) BbvI 51 GCTGC GCAGC(8/12) BpmI 656 CTCCAG CTGGAG(16/14) BsmFI 638 GTCCC GGGAC(10/14) Bsp1407I 310 TGTACA T^GTACA BspDI 275 ATCGAT AT^CGAT BspHI 197 TCATGA T^CATGA BsrDI 347 CATTGC GCAATG(2/0) BsrGI 310 TGTACA T^GTACA BsrI 501 ACTGG ACTGG(1/-1) BstYI 387 AGATCC [GA]^GATC[CT] Cac8I 47 GCCTGC GC[ACGT]^[ACGT]GC ClaI 275 ATCGAT AT^CGAT DpnII 388 GATC ^GATC EarI 292 GAAGAG CTCTTC(1/4) EcoT14I 608 CCAAGG C^C[AT][AT]GG EcoT22I 148 ATGCAT ATGCA^T Ksp632I 292 GAAGAG CTCTTC(1/4) MaeII 453 ACGT A^CGT MboI 388 GATC ^GATC MflI 387 AGATCC [GA]^GATC[CT] NdeI 884 CATATG CA^TATG NdeII 388 GATC ^GATC NsiI 148 ATGCAT ATGCA^T PleI 594 GACTC GAGTC(4/5) Psp1406I 452 AACGTT AA^CGTT RcaI 197 TCATGA T^CATGA Sau3AI 388 GATC ^GATC SexAI 415 ACCAGGT A^CC[AT]GGT SfaNI 470 GATGC GCATC(5/9) SspBI 310 TGTACA T^GTACA SspI 217 AATATT AAT^ATT StuI 834 AGGCCT AGG^CCT StyI 608 CCAAGG C^C[AT][AT]GG TseI 51 GCTGC G^C[AT]GC XhoII 387 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### Asp700I 537 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 690 GAACAGATTC AvaII 532 GGTCC G^G[AT]CC 637 GGTCC BsaJI 474 CCTGGG C^C[ACGT][ACGT]GG 608 CCAAGG BsmAI 496 GAGAC GTCTC(1/5) 650 GTCTC DdeI 89 CTGAG C^T[ACGT]AG 790 CTGAG Fnu4HI 51 GCTGC GC^[ACGT]GC 725 GCCGC HaeIII 181 GGCC GG^CC 835 GGCC ItaI 51 GCTGC GC^[ACGT]GC 725 GCCGC MwoI 28 GCACTTTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 163 GCATTCAAGGC NspI 24 ACATGC [GA]CATG^[CT] 796 GCATGT XmnI 537 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 690 GAACAGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AciI AcsI AfaI ApoI BsmI BssKI BstF5I BstNI Cfr13I EcoRII FokI HinfI MboII MnlI MseI MvaI NlaIII NlaIV RsaI Sau96I ScrFI TaqI TfiI Tru9I Tsp509I TspRI TthHB8I